Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody.
Identifieur interne : 001467 ( PubMed/Corpus ); précédent : 001466; suivant : 001468Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody.
Auteurs : Tianlei Ying ; Ponraj Prabakaran ; Lanying Du ; Wei Shi ; Yang Feng ; Yanping Wang ; Lingshu Wang ; Wei Li ; Shibo Jiang ; Dimiter S. Dimitrov ; Tongqing ZhouSource :
- Nature communications [ 2041-1723 ] ; 2015.
English descriptors
- KwdEn :
- Alleles, Antibodies, Neutralizing (genetics), Antibodies, Neutralizing (immunology), Antibodies, Viral (genetics), Antibodies, Viral (immunology), Crystallization, Crystallography, X-Ray, Epitopes, Genes, Immunoglobulin Heavy Chain (genetics), Genes, Immunoglobulin Heavy Chain (immunology), Humans, Immunoglobulin G (genetics), Immunoglobulin G (immunology), Middle East Respiratory Syndrome Coronavirus (immunology), Somatic Hypermutation, Immunoglobulin (genetics), Somatic Hypermutation, Immunoglobulin (immunology), V(D)J Recombination (genetics), V(D)J Recombination (immunology).
- MESH :
- chemical , genetics : Antibodies, Neutralizing, Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral, Immunoglobulin G.
- genetics : Genes, Immunoglobulin Heavy Chain, Somatic Hypermutation, Immunoglobulin, V(D)J Recombination.
- immunology : Genes, Immunoglobulin Heavy Chain, Middle East Respiratory Syndrome Coronavirus, Somatic Hypermutation, Immunoglobulin, V(D)J Recombination.
- Alleles, Crystallization, Crystallography, X-Ray, Epitopes, Humans.
Abstract
The MERS-CoV is an emerging virus, which already infected more than 1,300 humans with high (∼36%) mortality. Here, we show that m336, an exceptionally potent human anti-MERS-CoV antibody, is almost germline with only one somatic mutation in the heavy chain. The structure of Fab m336 in complex with the MERS-CoV receptor-binding domain reveals that its IGHV1-69-derived heavy chain provides more than 85% binding surface and that its epitope almost completely overlaps with the receptor-binding site. Analysis of antibodies from 69 healthy humans suggests an important role of the V(D)J recombination-generated junctional and allele-specific residues for achieving high affinity of binding at such low levels of somatic hypermutation. Our results also have important implications for development of vaccine immunogens based on the newly identified m336 epitope as well as for elucidation of mechanisms of neutralization by m336-like antibodies and their elicitation in vivo.
DOI: 10.1038/ncomms9223
PubMed: 26370782
Links to Exploration step
pubmed:26370782Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody.</title><author><name sortKey="Ying, Tianlei" sort="Ying, Tianlei" uniqKey="Ying T" first="Tianlei" last="Ying">Tianlei Ying</name><affiliation><nlm:affiliation>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Prabakaran, Ponraj" sort="Prabakaran, Ponraj" uniqKey="Prabakaran P" first="Ponraj" last="Prabakaran">Ponraj Prabakaran</name><affiliation><nlm:affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name><affiliation><nlm:affiliation>Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York 10065, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Wei" sort="Shi, Wei" uniqKey="Shi W" first="Wei" last="Shi">Wei Shi</name><affiliation><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Feng, Yang" sort="Feng, Yang" uniqKey="Feng Y" first="Yang" last="Feng">Yang Feng</name><affiliation><nlm:affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Yanping" sort="Wang, Yanping" uniqKey="Wang Y" first="Yanping" last="Wang">Yanping Wang</name><affiliation><nlm:affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Lingshu" sort="Wang, Lingshu" uniqKey="Wang L" first="Lingshu" last="Wang">Lingshu Wang</name><affiliation><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name><affiliation><nlm:affiliation>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><affiliation><nlm:affiliation>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Dimitrov, Dimiter S" sort="Dimitrov, Dimiter S" uniqKey="Dimitrov D" first="Dimiter S" last="Dimitrov">Dimiter S. Dimitrov</name><affiliation><nlm:affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Tongqing" sort="Zhou, Tongqing" uniqKey="Zhou T" first="Tongqing" last="Zhou">Tongqing Zhou</name><affiliation><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26370782</idno><idno type="pmid">26370782</idno><idno type="doi">10.1038/ncomms9223</idno><idno type="wicri:Area/PubMed/Corpus">001467</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001467</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody.</title><author><name sortKey="Ying, Tianlei" sort="Ying, Tianlei" uniqKey="Ying T" first="Tianlei" last="Ying">Tianlei Ying</name><affiliation><nlm:affiliation>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Prabakaran, Ponraj" sort="Prabakaran, Ponraj" uniqKey="Prabakaran P" first="Ponraj" last="Prabakaran">Ponraj Prabakaran</name><affiliation><nlm:affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name><affiliation><nlm:affiliation>Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York 10065, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Wei" sort="Shi, Wei" uniqKey="Shi W" first="Wei" last="Shi">Wei Shi</name><affiliation><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Feng, Yang" sort="Feng, Yang" uniqKey="Feng Y" first="Yang" last="Feng">Yang Feng</name><affiliation><nlm:affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Yanping" sort="Wang, Yanping" uniqKey="Wang Y" first="Yanping" last="Wang">Yanping Wang</name><affiliation><nlm:affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Lingshu" sort="Wang, Lingshu" uniqKey="Wang L" first="Lingshu" last="Wang">Lingshu Wang</name><affiliation><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name><affiliation><nlm:affiliation>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><affiliation><nlm:affiliation>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.</nlm:affiliation></affiliation></author><author><name sortKey="Dimitrov, Dimiter S" sort="Dimitrov, Dimiter S" uniqKey="Dimitrov D" first="Dimiter S" last="Dimitrov">Dimiter S. Dimitrov</name><affiliation><nlm:affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Tongqing" sort="Zhou, Tongqing" uniqKey="Zhou T" first="Tongqing" last="Zhou">Tongqing Zhou</name><affiliation><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Nature communications</title><idno type="eISSN">2041-1723</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alleles</term><term>Antibodies, Neutralizing (genetics)</term><term>Antibodies, Neutralizing (immunology)</term><term>Antibodies, Viral (genetics)</term><term>Antibodies, Viral (immunology)</term><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Epitopes</term><term>Genes, Immunoglobulin Heavy Chain (genetics)</term><term>Genes, Immunoglobulin Heavy Chain (immunology)</term><term>Humans</term><term>Immunoglobulin G (genetics)</term><term>Immunoglobulin G (immunology)</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Somatic Hypermutation, Immunoglobulin (genetics)</term><term>Somatic Hypermutation, Immunoglobulin (immunology)</term><term>V(D)J Recombination (genetics)</term><term>V(D)J Recombination (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Immunoglobulin Heavy Chain</term><term>Somatic Hypermutation, Immunoglobulin</term><term>V(D)J Recombination</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Genes, Immunoglobulin Heavy Chain</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Somatic Hypermutation, Immunoglobulin</term><term>V(D)J Recombination</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alleles</term><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Epitopes</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The MERS-CoV is an emerging virus, which already infected more than 1,300 humans with high (∼36%) mortality. Here, we show that m336, an exceptionally potent human anti-MERS-CoV antibody, is almost germline with only one somatic mutation in the heavy chain. The structure of Fab m336 in complex with the MERS-CoV receptor-binding domain reveals that its IGHV1-69-derived heavy chain provides more than 85% binding surface and that its epitope almost completely overlaps with the receptor-binding site. Analysis of antibodies from 69 healthy humans suggests an important role of the V(D)J recombination-generated junctional and allele-specific residues for achieving high affinity of binding at such low levels of somatic hypermutation. Our results also have important implications for development of vaccine immunogens based on the newly identified m336 epitope as well as for elucidation of mechanisms of neutralization by m336-like antibodies and their elicitation in vivo. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26370782</PMID><DateCompleted><Year>2016</Year><Month>04</Month><Day>26</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>08</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2041-1723</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><PubDate><Year>2015</Year><Month>Sep</Month><Day>15</Day></PubDate></JournalIssue><Title>Nature communications</Title><ISOAbbreviation>Nat Commun</ISOAbbreviation></Journal><ArticleTitle>Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody.</ArticleTitle><Pagination><MedlinePgn>8223</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/ncomms9223</ELocationID><Abstract><AbstractText>The MERS-CoV is an emerging virus, which already infected more than 1,300 humans with high (∼36%) mortality. Here, we show that m336, an exceptionally potent human anti-MERS-CoV antibody, is almost germline with only one somatic mutation in the heavy chain. The structure of Fab m336 in complex with the MERS-CoV receptor-binding domain reveals that its IGHV1-69-derived heavy chain provides more than 85% binding surface and that its epitope almost completely overlaps with the receptor-binding site. Analysis of antibodies from 69 healthy humans suggests an important role of the V(D)J recombination-generated junctional and allele-specific residues for achieving high affinity of binding at such low levels of somatic hypermutation. Our results also have important implications for development of vaccine immunogens based on the newly identified m336 epitope as well as for elucidation of mechanisms of neutralization by m336-like antibodies and their elicitation in vivo. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ying</LastName><ForeName>Tianlei</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Prabakaran</LastName><ForeName>Ponraj</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Lanying</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York 10065, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Yang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yanping</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Lingshu</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Shibo</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York 10065, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dimitrov</LastName><ForeName>Dimiter S</ForeName><Initials>DS</Initials><AffiliationInfo><Affiliation>Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Tongqing</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>PDB</DataBankName><AccessionNumberList><AccessionNumber>4XAK</AccessionNumber></AccessionNumberList></DataBank></DataBankList><GrantList CompleteYN="Y"><Grant><GrantID>Z99 CA999999</GrantID><Agency>Intramural NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052060">Research Support, N.I.H., Intramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>09</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nat Commun</MedlineTA><NlmUniqueID>101528555</NlmUniqueID><ISSNLinking>2041-1723</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D057134">Antibodies, Neutralizing</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000939">Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000483" MajorTopicYN="N">Alleles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D057134" MajorTopicYN="N">Antibodies, Neutralizing</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003460" MajorTopicYN="N">Crystallization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050438" MajorTopicYN="N">Genes, Immunoglobulin Heavy Chain</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027041" MajorTopicYN="N">Somatic Hypermutation, Immunoglobulin</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060152" MajorTopicYN="N">V(D)J Recombination</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>04</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>07</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>9</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>9</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>4</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26370782</ArticleId><ArticleId IdType="pii">ncomms9223</ArticleId><ArticleId IdType="doi">10.1038/ncomms9223</ArticleId><ArticleId IdType="pmc">PMC4571279</ArticleId><ArticleId IdType="mid">NIHMS712496</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Immunity. 1999 Sep;11(3):329-38</Citation><ArticleIdList><ArticleId IdType="pubmed">10514011</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2002 Nov;58(Pt 11):1948-54</Citation><ArticleIdList><ArticleId IdType="pubmed">12393927</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2706-11</Citation><ArticleIdList><ArticleId IdType="pubmed">14981267</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):760-3</Citation><ArticleIdList><ArticleId IdType="pubmed">15299374</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32</Citation><ArticleIdList><ArticleId IdType="pubmed">15572765</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Immunol. 2005 Jul;35(7):2131-45</Citation><ArticleIdList><ArticleId IdType="pubmed">15971273</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2006 Jun 9;281(23):15829-36</Citation><ArticleIdList><ArticleId IdType="pubmed">16597622</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2007 Jul;35(Web Server issue):W375-83</Citation><ArticleIdList><ArticleId IdType="pubmed">17452350</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12123-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17620608</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W503-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18503082</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Mol Biol. 2009 Mar;16(3):265-73</Citation><ArticleIdList><ArticleId IdType="pubmed">19234466</ArticleId></ArticleIdList></Reference><Reference><Citation>J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674</Citation><ArticleIdList><ArticleId IdType="pubmed">19461840</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2009 Dec 18;390(3):404-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19748484</ArticleId></ArticleIdList></Reference><Reference><Citation>MAbs. 2010 May-Jun;2(3):347-56</Citation><ArticleIdList><ArticleId IdType="pubmed">20400863</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2010 Aug 13;329(5993):811-7</Citation><ArticleIdList><ArticleId IdType="pubmed">20616231</ArticleId></ArticleIdList></Reference><Reference><Citation>Viruses. 2010 Feb;2(2):547-565</Citation><ArticleIdList><ArticleId IdType="pubmed">21755021</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2011 Sep 16;333(6049):1593-602</Citation><ArticleIdList><ArticleId IdType="pubmed">21835983</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunogenetics. 2012 May;64(5):337-50</Citation><ArticleIdList><ArticleId IdType="pubmed">22200891</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2012 May 07;30(5):423-33</Citation><ArticleIdList><ArticleId IdType="pubmed">22565972</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2012 Jul 13;337(6091):183-6</Citation><ArticleIdList><ArticleId IdType="pubmed">22798606</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2012 Aug 02;3:277</Citation><ArticleIdList><ArticleId IdType="pubmed">22876240</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2012 Sep 27;489(7417):566-70</Citation><ArticleIdList><ArticleId IdType="pubmed">22932267</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2012 Nov 20;3(6):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2013 Jan;9(1):e1003106</Citation><ArticleIdList><ArticleId IdType="pubmed">23300456</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation><ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2013 May 10;340(6133):711-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23539181</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Aug;87(15):8638-50</Citation><ArticleIdList><ArticleId IdType="pubmed">23720729</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2013 Aug 1;369(5):407-16</Citation><ArticleIdList><ArticleId IdType="pubmed">23782161</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Res. 2013 Aug;23(8):986-93</Citation><ArticleIdList><ArticleId IdType="pubmed">23835475</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunity. 2013 Aug 22;39(2):245-58</Citation><ArticleIdList><ArticleId IdType="pubmed">23911655</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet Infect Dis. 2013 Oct;13(10):859-66</Citation><ArticleIdList><ArticleId IdType="pubmed">23933067</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2013;4:2333</Citation><ArticleIdList><ArticleId IdType="pubmed">23995877</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2013;9(10):e1003684</Citation><ArticleIdList><ArticleId IdType="pubmed">24130486</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2013 Dec;11(12):836-48</Citation><ArticleIdList><ArticleId IdType="pubmed">24217413</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Transl Med. 2013 Nov 27;5(213):213ra167</Citation><ArticleIdList><ArticleId IdType="pubmed">24285486</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013 Dec 12;18(50):20662</Citation><ArticleIdList><ArticleId IdType="pubmed">24342516</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013 Dec 12;18(50):20659</Citation><ArticleIdList><ArticleId IdType="pubmed">24342517</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2014 May;88(9):4953-61</Citation><ArticleIdList><ArticleId IdType="pubmed">24554656</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 May 13;111(19):E2018-26</Citation><ArticleIdList><ArticleId IdType="pubmed">24778221</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Transl Med. 2014 Apr 30;6(234):234ra59</Citation><ArticleIdList><ArticleId IdType="pubmed">24778414</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2014 Jul;88(14):7796-805</Citation><ArticleIdList><ArticleId IdType="pubmed">24789777</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Immunol. 2014 Apr 22;5:170</Citation><ArticleIdList><ArticleId IdType="pubmed">24795717</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Transl Med. 2014 Jun 25;6(242):242ra82</Citation><ArticleIdList><ArticleId IdType="pubmed">24964990</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12516-21</Citation><ArticleIdList><ArticleId IdType="pubmed">25114257</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2014 Dec 18;516(7531):418-22</Citation><ArticleIdList><ArticleId IdType="pubmed">25296253</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbes Infect. 2015 Feb;17(2):142-8</Citation><ArticleIdList><ArticleId IdType="pubmed">25456101</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2014 Dec 12;346(6215):1380-1383</Citation><ArticleIdList><ArticleId IdType="pubmed">25504724</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2015 Jul 28;6:7712</Citation><ArticleIdList><ArticleId IdType="pubmed">26218507</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 1997;276:307-26</Citation><ArticleIdList><ArticleId IdType="pubmed">27754618</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001467 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 001467 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:26370782
|texte= Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:26370782" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |