Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein.
Identifieur interne : 002984 ( PubMed/Checkpoint ); précédent : 002983; suivant : 002985Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein.
Auteurs : T A Ozharovskaia [Russie] ; O V Zubkova [Russie] ; I V Dolzhikova [Russie] ; A S Gromova [Russie] ; D M Grousova [Russie] ; A I Tukhvatulin [Russie] ; O. Popova [Russie] ; D V Shcheblyakov [Russie] ; D N Scherbinin [Russie] ; A S Dzharullaeva [Russie] ; A S Erokhova [Russie] ; M M Shmarov [Russie] ; S Y Loginova [Russie] ; S V Borisevich [Russie] ; B S Naroditsky [Russie] ; D Y Logunov [Russie] ; A L Gintsburg [Russie]Source :
- Acta naturae [ 2075-8251 ]
Abstract
The Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in 2012 during the first Middle East respiratory syndrome (MERS) outbreaks. MERS-CoV causes an acute lower-respiratory infection in humans, with a fatality rate of ~35.5%. Currently, there are no registered vaccines or means of therapeutic protection against MERS in the world. The MERS-CoV S glycoprotein plays the most important role in the viral life cycle (virus internalization). The S protein is an immunodominant antigen and the main target for neutralizing antibodies. In the present study, the immunogenicities of five different forms of the MERS-CoV S glycoprotein were compared: the full-length S glycoprotein, the full-length S glycoprotein with the transmembrane domain of the G glycoprotein of VSV (S-G), the receptor-binding domain (RBD) of the S glycoprotein, the membrane-fused RBD (the RBD fused with the transmembrane domain of the VSV G glycoprotein (RBD-G)), and the RBD fused with Fc of human IgG1 (RBD-Fc). Recombinant vectors based on human adenoviruses type 5 (rAd5) were used as delivery vehicles. Vaccination with all of the developed rAd5 vectors elicited a balanced Th1/Th2 response in mice. The most robust humoral immune response was induced after the animal had been vaccinated with the membrane-fused RBD (rAd5-RBD-G). Only immunization with membrane forms of the glycoprotein (rAd5-S, rAd5-S-G, and rAd5-RBD-G) elicited neutralizing antibodies among all vaccinated animals. The most significant cellular immune response was induced after vaccination of the animals with the full-length S (rAd5-S). These investigations suggest that the full-length S and the membrane form of the RBD (RBD-G) are the most promising vaccine candidates among all the studied forms of S glycoprotein.
PubMed: 31024747
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F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Logunov, D Y" sort="Logunov, D Y" uniqKey="Logunov D" first="D Y" last="Logunov">D Y Logunov</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Gintsburg, A L" sort="Gintsburg, A L" uniqKey="Gintsburg A" first="A L" last="Gintsburg">A L Gintsburg</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. 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F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Zubkova, O V" sort="Zubkova, O V" uniqKey="Zubkova O" first="O V" last="Zubkova">O V Zubkova</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Dolzhikova, I V" sort="Dolzhikova, I V" uniqKey="Dolzhikova I" first="I V" last="Dolzhikova">I V Dolzhikova</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Gromova, A S" sort="Gromova, A S" uniqKey="Gromova A" first="A S" last="Gromova">A S Gromova</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Grousova, D M" sort="Grousova, D M" uniqKey="Grousova D" first="D M" last="Grousova">D M Grousova</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Tukhvatulin, A I" sort="Tukhvatulin, A I" uniqKey="Tukhvatulin A" first="A I" last="Tukhvatulin">A I Tukhvatulin</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Popova, O" sort="Popova, O" uniqKey="Popova O" first="O" last="Popova">O. Popova</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Shcheblyakov, D V" sort="Shcheblyakov, D V" uniqKey="Shcheblyakov D" first="D V" last="Shcheblyakov">D V Shcheblyakov</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Scherbinin, D N" sort="Scherbinin, D N" uniqKey="Scherbinin D" first="D N" last="Scherbinin">D N Scherbinin</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Dzharullaeva, A S" sort="Dzharullaeva, A S" uniqKey="Dzharullaeva A" first="A S" last="Dzharullaeva">A S Dzharullaeva</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Erokhova, A S" sort="Erokhova, A S" uniqKey="Erokhova A" first="A S" last="Erokhova">A S Erokhova</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Shmarov, M M" sort="Shmarov, M M" uniqKey="Shmarov M" first="M M" last="Shmarov">M M Shmarov</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Loginova, S Y" sort="Loginova, S Y" uniqKey="Loginova S" first="S Y" last="Loginova">S Y Loginova</name><affiliation wicri:level="1"><nlm:affiliation>«48 Central Research Institute», Ministry of Defense of Russian Federation, Oktyabrskaya Str. 11, 141306 , Sergiev Posad, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>«48 Central Research Institute», Ministry of Defense of Russian Federation, Oktyabrskaya Str. 11, 141306 , Sergiev Posad</wicri:regionArea><wicri:noRegion>Sergiev Posad</wicri:noRegion></affiliation></author><author><name sortKey="Borisevich, S V" sort="Borisevich, S V" uniqKey="Borisevich S" first="S V" last="Borisevich">S V Borisevich</name><affiliation wicri:level="1"><nlm:affiliation>«48 Central Research Institute», Ministry of Defense of Russian Federation, Oktyabrskaya Str. 11, 141306 , Sergiev Posad, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>«48 Central Research Institute», Ministry of Defense of Russian Federation, Oktyabrskaya Str. 11, 141306 , Sergiev Posad</wicri:regionArea><wicri:noRegion>Sergiev Posad</wicri:noRegion></affiliation></author><author><name sortKey="Naroditsky, B S" sort="Naroditsky, B S" uniqKey="Naroditsky B" first="B S" last="Naroditsky">B S Naroditsky</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Logunov, D Y" sort="Logunov, D Y" uniqKey="Logunov D" first="D Y" last="Logunov">D Y Logunov</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Gintsburg, A L" sort="Gintsburg, A L" uniqKey="Gintsburg A" first="A L" last="Gintsburg">A L Gintsburg</name><affiliation wicri:level="3"><nlm:affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author></analytic><series><title level="j">Acta naturae</title><idno type="ISSN">2075-8251</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in 2012 during the first Middle East respiratory syndrome (MERS) outbreaks. MERS-CoV causes an acute lower-respiratory infection in humans, with a fatality rate of ~35.5%. Currently, there are no registered vaccines or means of therapeutic protection against MERS in the world. The MERS-CoV S glycoprotein plays the most important role in the viral life cycle (virus internalization). The S protein is an immunodominant antigen and the main target for neutralizing antibodies. In the present study, the immunogenicities of five different forms of the MERS-CoV S glycoprotein were compared: the full-length S glycoprotein, the full-length S glycoprotein with the transmembrane domain of the G glycoprotein of VSV (S-G), the receptor-binding domain (RBD) of the S glycoprotein, the membrane-fused RBD (the RBD fused with the transmembrane domain of the VSV G glycoprotein (RBD-G)), and the RBD fused with Fc of human IgG1 (RBD-Fc). Recombinant vectors based on human adenoviruses type 5 (rAd5) were used as delivery vehicles. Vaccination with all of the developed rAd5 vectors elicited a balanced Th1/Th2 response in mice. The most robust humoral immune response was induced after the animal had been vaccinated with the membrane-fused RBD (rAd5-RBD-G). Only immunization with membrane forms of the glycoprotein (rAd5-S, rAd5-S-G, and rAd5-RBD-G) elicited neutralizing antibodies among all vaccinated animals. The most significant cellular immune response was induced after vaccination of the animals with the full-length S (rAd5-S). These investigations suggest that the full-length S and the membrane form of the RBD (RBD-G) are the most promising vaccine candidates among all the studied forms of S glycoprotein.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31024747</PMID><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">2075-8251</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><Issue>1</Issue><PubDate><MedlineDate>2019 Jan-Mar</MedlineDate></PubDate></JournalIssue><Title>Acta naturae</Title><ISOAbbreviation>Acta Naturae</ISOAbbreviation></Journal><ArticleTitle>Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein.</ArticleTitle><Pagination><MedlinePgn>38-47</MedlinePgn></Pagination><Abstract><AbstractText>The Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in 2012 during the first Middle East respiratory syndrome (MERS) outbreaks. MERS-CoV causes an acute lower-respiratory infection in humans, with a fatality rate of ~35.5%. Currently, there are no registered vaccines or means of therapeutic protection against MERS in the world. The MERS-CoV S glycoprotein plays the most important role in the viral life cycle (virus internalization). The S protein is an immunodominant antigen and the main target for neutralizing antibodies. In the present study, the immunogenicities of five different forms of the MERS-CoV S glycoprotein were compared: the full-length S glycoprotein, the full-length S glycoprotein with the transmembrane domain of the G glycoprotein of VSV (S-G), the receptor-binding domain (RBD) of the S glycoprotein, the membrane-fused RBD (the RBD fused with the transmembrane domain of the VSV G glycoprotein (RBD-G)), and the RBD fused with Fc of human IgG1 (RBD-Fc). Recombinant vectors based on human adenoviruses type 5 (rAd5) were used as delivery vehicles. Vaccination with all of the developed rAd5 vectors elicited a balanced Th1/Th2 response in mice. The most robust humoral immune response was induced after the animal had been vaccinated with the membrane-fused RBD (rAd5-RBD-G). Only immunization with membrane forms of the glycoprotein (rAd5-S, rAd5-S-G, and rAd5-RBD-G) elicited neutralizing antibodies among all vaccinated animals. The most significant cellular immune response was induced after vaccination of the animals with the full-length S (rAd5-S). These investigations suggest that the full-length S and the membrane form of the RBD (RBD-G) are the most promising vaccine candidates among all the studied forms of S glycoprotein.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ozharovskaia</LastName><ForeName>T A</ForeName><Initials>TA</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zubkova</LastName><ForeName>O V</ForeName><Initials>OV</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dolzhikova</LastName><ForeName>I V</ForeName><Initials>IV</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gromova</LastName><ForeName>A S</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grousova</LastName><ForeName>D M</ForeName><Initials>DM</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tukhvatulin</LastName><ForeName>A I</ForeName><Initials>AI</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Popova</LastName><ForeName>O</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shcheblyakov</LastName><ForeName>D V</ForeName><Initials>DV</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scherbinin</LastName><ForeName>D N</ForeName><Initials>DN</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dzharullaeva</LastName><ForeName>A S</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Erokhova</LastName><ForeName>A S</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shmarov</LastName><ForeName>M M</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Loginova</LastName><ForeName>S Y</ForeName><Initials>SY</Initials><AffiliationInfo><Affiliation>«48 Central Research Institute», Ministry of Defense of Russian Federation, Oktyabrskaya Str. 11, 141306 , Sergiev Posad, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Borisevich</LastName><ForeName>S V</ForeName><Initials>SV</Initials><AffiliationInfo><Affiliation>«48 Central Research Institute», Ministry of Defense of Russian Federation, Oktyabrskaya Str. 11, 141306 , Sergiev Posad, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Naroditsky</LastName><ForeName>B S</ForeName><Initials>BS</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Logunov</LastName><ForeName>D Y</ForeName><Initials>DY</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gintsburg</LastName><ForeName>A L</ForeName><Initials>AL</Initials><AffiliationInfo><Affiliation>Federal Research Centre of Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, Ministry of Health of Russian Federation, Gamaleya Str. 18, 123098, Moscow, Russia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Russia (Federation)</Country><MedlineTA>Acta Naturae</MedlineTA><NlmUniqueID>101525823</NlmUniqueID><ISSNLinking>2075-8251</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MERS</Keyword><Keyword MajorTopicYN="N">MERS-CoV</Keyword><Keyword MajorTopicYN="N">Middle East respiratory syndrome</Keyword><Keyword MajorTopicYN="N">adenoviral vector</Keyword><Keyword MajorTopicYN="N">glycoprotein</Keyword><Keyword MajorTopicYN="N">immunity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>4</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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S Dzharullaeva</name><name sortKey="Erokhova, A S" sort="Erokhova, A S" uniqKey="Erokhova A" first="A S" last="Erokhova">A S Erokhova</name><name sortKey="Gintsburg, A L" sort="Gintsburg, A L" uniqKey="Gintsburg A" first="A L" last="Gintsburg">A L Gintsburg</name><name sortKey="Gromova, A S" sort="Gromova, A S" uniqKey="Gromova A" first="A S" last="Gromova">A S Gromova</name><name sortKey="Grousova, D M" sort="Grousova, D M" uniqKey="Grousova D" first="D M" last="Grousova">D M Grousova</name><name sortKey="Loginova, S Y" sort="Loginova, S Y" uniqKey="Loginova S" first="S Y" last="Loginova">S Y Loginova</name><name sortKey="Logunov, D Y" sort="Logunov, D Y" uniqKey="Logunov D" first="D Y" last="Logunov">D Y Logunov</name><name sortKey="Naroditsky, B S" sort="Naroditsky, B S" uniqKey="Naroditsky B" first="B S" last="Naroditsky">B S Naroditsky</name><name sortKey="Popova, O" sort="Popova, O" uniqKey="Popova O" first="O" last="Popova">O. Popova</name><name sortKey="Scherbinin, D N" sort="Scherbinin, D N" uniqKey="Scherbinin D" first="D N" last="Scherbinin">D N Scherbinin</name><name sortKey="Shcheblyakov, D V" sort="Shcheblyakov, D V" uniqKey="Shcheblyakov D" first="D V" last="Shcheblyakov">D V Shcheblyakov</name><name sortKey="Shmarov, M M" sort="Shmarov, M M" uniqKey="Shmarov M" first="M M" last="Shmarov">M M Shmarov</name><name sortKey="Tukhvatulin, A I" sort="Tukhvatulin, A I" uniqKey="Tukhvatulin A" first="A I" last="Tukhvatulin">A I Tukhvatulin</name><name sortKey="Zubkova, O V" sort="Zubkova, O V" uniqKey="Zubkova O" first="O V" last="Zubkova">O V Zubkova</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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