Subunit sequences of the 4 x 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata.
Identifieur interne : 002297 ( PubMed/Checkpoint ); précédent : 002296; suivant : 002298Subunit sequences of the 4 x 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata.
Auteurs : Anne Averdam [Allemagne] ; Jürgen Markl ; Thorsten BurmesterSource :
- European journal of biochemistry [ 0014-2956 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Hemocyanins.
- chemical , genetics : Hemocyanins.
- chemical , metabolism : Hemocyanins.
- chemistry : Spiders.
- genetics : Spiders.
- Amino Acid Sequence, Animals, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Evolution, Molecular, Immunoelectrophoresis, Molecular Sequence Data, Phylogeny.
Abstract
The transport of oxygen in the hemolymph of many arthropod and mollusc species is mediated by large copper-proteins that are referred to as hemocyanins. Arthropod hemocyanins are composed of hexamers and oligomers of hexamers. Arachnid hemocyanins usually form 4 x 6-mers consisting of seven distinct subunit types (termed a-g), although in some spider taxa deviations from this standard scheme have been observed. Applying immunological and electrophoretic methods, six distinct hemocyanin subunits were identified in the red-legged golden orb-web spider Nephila inaurata madagascariensis (Araneae: Tetragnathidae). The complete cDNA sequences of six subunits were obtained that corresponded to a-, b-, d-, e-, f- and g-type subunits. No evidence for a c-type subunit was found in this species. The inclusion of the N. inaurata hemocyanins in a multiple alignment of the arthropod hemocyanins and the application of the Bayesian method of phylogenetic inference allow, for the first time, a solid reconstruction of the intramolecular evolution of the chelicerate hemocyanin subunits. The branch leading to subunit a diverged first, followed by the common branch of the dimer-forming b and c subunits, while subunits d and f, as well as subunits e and g form common branches. Assuming a clock-like evolution of the chelicerate hemocyanins, a timescale for the evolution of the Chelicerata was obtained that agrees with the fossil record.
DOI: 10.1046/j.1432-1033.2003.03730.x
PubMed: 12899700
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:12899700Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Subunit sequences of the 4 x 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata.</title><author><name sortKey="Averdam, Anne" sort="Averdam, Anne" uniqKey="Averdam A" first="Anne" last="Averdam">Anne Averdam</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Zoology, Johannes Gutenberg University, Mainz, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Zoology, Johannes Gutenberg University, Mainz</wicri:regionArea><placeName><region type="land" nuts="2">Rhénanie-Palatinat</region><settlement type="city">Mayence</settlement></placeName></affiliation></author><author><name sortKey="Markl, Jurgen" sort="Markl, Jurgen" uniqKey="Markl J" first="Jürgen" last="Markl">Jürgen Markl</name></author><author><name sortKey="Burmester, Thorsten" sort="Burmester, Thorsten" uniqKey="Burmester T" first="Thorsten" last="Burmester">Thorsten Burmester</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12899700</idno><idno type="pmid">12899700</idno><idno type="doi">10.1046/j.1432-1033.2003.03730.x</idno><idno type="wicri:Area/PubMed/Corpus">002442</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002442</idno><idno type="wicri:Area/PubMed/Curation">002442</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002442</idno><idno type="wicri:Area/PubMed/Checkpoint">002297</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002297</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Subunit sequences of the 4 x 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata.</title><author><name sortKey="Averdam, Anne" sort="Averdam, Anne" uniqKey="Averdam A" first="Anne" last="Averdam">Anne Averdam</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Zoology, Johannes Gutenberg University, Mainz, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Zoology, Johannes Gutenberg University, Mainz</wicri:regionArea><placeName><region type="land" nuts="2">Rhénanie-Palatinat</region><settlement type="city">Mayence</settlement></placeName></affiliation></author><author><name sortKey="Markl, Jurgen" sort="Markl, Jurgen" uniqKey="Markl J" first="Jürgen" last="Markl">Jürgen Markl</name></author><author><name sortKey="Burmester, Thorsten" sort="Burmester, Thorsten" uniqKey="Burmester T" first="Thorsten" last="Burmester">Thorsten Burmester</name></author></analytic><series><title level="j">European journal of biochemistry</title><idno type="ISSN">0014-2956</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Blotting, Western</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Evolution, Molecular</term><term>Hemocyanins (chemistry)</term><term>Hemocyanins (genetics)</term><term>Hemocyanins (metabolism)</term><term>Immunoelectrophoresis</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Spiders (chemistry)</term><term>Spiders (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Araignées ()</term><term>Araignées (génétique)</term><term>Données de séquences moléculaires</term><term>Immunoélectrophorèse</term><term>Phylogénie</term><term>Séquence d'acides aminés</term><term>Technique de Western</term><term>Électrophorèse sur gel de polyacrylamide</term><term>Évolution moléculaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Hemocyanins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemocyanins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hemocyanins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Spiders</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Spiders</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Araignées</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Blotting, Western</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Evolution, Molecular</term><term>Immunoelectrophoresis</term><term>Molecular Sequence Data</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Araignées</term><term>Données de séquences moléculaires</term><term>Immunoélectrophorèse</term><term>Phylogénie</term><term>Séquence d'acides aminés</term><term>Technique de Western</term><term>Électrophorèse sur gel de polyacrylamide</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The transport of oxygen in the hemolymph of many arthropod and mollusc species is mediated by large copper-proteins that are referred to as hemocyanins. Arthropod hemocyanins are composed of hexamers and oligomers of hexamers. Arachnid hemocyanins usually form 4 x 6-mers consisting of seven distinct subunit types (termed a-g), although in some spider taxa deviations from this standard scheme have been observed. Applying immunological and electrophoretic methods, six distinct hemocyanin subunits were identified in the red-legged golden orb-web spider Nephila inaurata madagascariensis (Araneae: Tetragnathidae). The complete cDNA sequences of six subunits were obtained that corresponded to a-, b-, d-, e-, f- and g-type subunits. No evidence for a c-type subunit was found in this species. The inclusion of the N. inaurata hemocyanins in a multiple alignment of the arthropod hemocyanins and the application of the Bayesian method of phylogenetic inference allow, for the first time, a solid reconstruction of the intramolecular evolution of the chelicerate hemocyanin subunits. The branch leading to subunit a diverged first, followed by the common branch of the dimer-forming b and c subunits, while subunits d and f, as well as subunits e and g form common branches. Assuming a clock-like evolution of the chelicerate hemocyanins, a timescale for the evolution of the Chelicerata was obtained that agrees with the fossil record.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12899700</PMID><DateCompleted><Year>2003</Year><Month>10</Month><Day>06</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0014-2956</ISSN><JournalIssue CitedMedium="Print"><Volume>270</Volume><Issue>16</Issue><PubDate><Year>2003</Year><Month>Aug</Month></PubDate></JournalIssue><Title>European journal of biochemistry</Title><ISOAbbreviation>Eur. J. Biochem.</ISOAbbreviation></Journal><ArticleTitle>Subunit sequences of the 4 x 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata.</ArticleTitle><Pagination><MedlinePgn>3432-9</MedlinePgn></Pagination><Abstract><AbstractText>The transport of oxygen in the hemolymph of many arthropod and mollusc species is mediated by large copper-proteins that are referred to as hemocyanins. Arthropod hemocyanins are composed of hexamers and oligomers of hexamers. Arachnid hemocyanins usually form 4 x 6-mers consisting of seven distinct subunit types (termed a-g), although in some spider taxa deviations from this standard scheme have been observed. Applying immunological and electrophoretic methods, six distinct hemocyanin subunits were identified in the red-legged golden orb-web spider Nephila inaurata madagascariensis (Araneae: Tetragnathidae). The complete cDNA sequences of six subunits were obtained that corresponded to a-, b-, d-, e-, f- and g-type subunits. No evidence for a c-type subunit was found in this species. The inclusion of the N. inaurata hemocyanins in a multiple alignment of the arthropod hemocyanins and the application of the Bayesian method of phylogenetic inference allow, for the first time, a solid reconstruction of the intramolecular evolution of the chelicerate hemocyanin subunits. The branch leading to subunit a diverged first, followed by the common branch of the dimer-forming b and c subunits, while subunits d and f, as well as subunits e and g form common branches. Assuming a clock-like evolution of the chelicerate hemocyanins, a timescale for the evolution of the Chelicerata was obtained that agrees with the fossil record.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Averdam</LastName><ForeName>Anne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Zoology, Johannes Gutenberg University, Mainz, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Markl</LastName><ForeName>Jürgen</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Burmester</LastName><ForeName>Thorsten</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Eur J Biochem</MedlineTA><NlmUniqueID>0107600</NlmUniqueID><ISSNLinking>0014-2956</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9013-72-3</RegistryNumber><NameOfSubstance UI="D006433">Hemocyanins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="N">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006433" MajorTopicYN="N">Hemocyanins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007122" MajorTopicYN="N">Immunoelectrophoresis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013112" MajorTopicYN="N">Spiders</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>8</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>10</Month><Day>8</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>8</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12899700</ArticleId><ArticleId IdType="pii">3730</ArticleId><ArticleId IdType="doi">10.1046/j.1432-1033.2003.03730.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Rhénanie-Palatinat</li></region><settlement><li>Mayence</li></settlement></list><tree><noCountry><name sortKey="Burmester, Thorsten" sort="Burmester, Thorsten" uniqKey="Burmester T" first="Thorsten" last="Burmester">Thorsten Burmester</name><name sortKey="Markl, Jurgen" sort="Markl, Jurgen" uniqKey="Markl J" first="Jürgen" last="Markl">Jürgen Markl</name></noCountry><country name="Allemagne"><region name="Rhénanie-Palatinat"><name sortKey="Averdam, Anne" sort="Averdam, Anne" uniqKey="Averdam A" first="Anne" last="Averdam">Anne Averdam</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002297 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002297 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:12899700
|texte= Subunit sequences of the 4 x 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:12899700" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |