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Real time metagenomics: using k-mers to annotate metagenomes.

Identifieur interne : 001C41 ( PubMed/Checkpoint ); précédent : 001C40; suivant : 001C42

Real time metagenomics: using k-mers to annotate metagenomes.

Auteurs : Robert A. Edwards [États-Unis] ; Robert Olson ; Terry Disz ; Gordon D. Pusch ; Veronika Vonstein ; Rick Stevens ; Ross Overbeek

Source :

RBID : pubmed:23047562

Descripteurs français

English descriptors

Abstract

Annotation of metagenomes involves comparing the individual sequence reads with a database of known sequences and assigning a unique function to each read. This is a time-consuming task that is computationally intensive (though not computationally complex). Here we present a novel approach to annotate metagenomes using unique k-mer oligopeptide sequences from 7 to 12 amino acids long. We demonstrate that k-mer-based annotations are faster and approach the sensitivity and precision of blastx-based annotations without loosing accuracy. A last-common ancestor approach was also developed to describe the members of the community.

DOI: 10.1093/bioinformatics/bts599
PubMed: 23047562


Affiliations:


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pubmed:23047562

Le document en format XML

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<Citation>Nucleic Acids Res. 2009 Nov;37(20):6643-54</Citation>
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