Heat inactivation of the Middle East respiratory syndrome coronavirus.
Identifieur interne : 001894 ( PubMed/Checkpoint ); précédent : 001893; suivant : 001895Heat inactivation of the Middle East respiratory syndrome coronavirus.
Auteurs : India Leclercq [France] ; Christophe Batéjat ; Ana M. Burguière ; Jean-Claude ManuguerraSource :
- Influenza and other respiratory viruses [ 1750-2659 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemistry : Middle East Respiratory Syndrome Coronavirus.
- methods : Disinfection.
- physiology : Middle East Respiratory Syndrome Coronavirus.
- virology : Coronavirus Infections.
- Hot Temperature, Humans.
Abstract
The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log10 . Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.
DOI: 10.1111/irv.12261
PubMed: 25074677
Affiliations:
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Burguière</name></author><author><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25074677</idno><idno type="pmid">25074677</idno><idno type="doi">10.1111/irv.12261</idno><idno type="wicri:Area/PubMed/Corpus">001896</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001896</idno><idno type="wicri:Area/PubMed/Curation">001896</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001896</idno><idno type="wicri:Area/PubMed/Checkpoint">001894</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001894</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Heat inactivation of the Middle East respiratory syndrome coronavirus.</title><author><name sortKey="Leclercq, India" sort="Leclercq, India" uniqKey="Leclercq I" first="India" last="Leclercq">India Leclercq</name><affiliation wicri:level="3"><nlm:affiliation>Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur, Paris, France; Université Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur, Paris, France; Université Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Batejat, Christophe" sort="Batejat, Christophe" uniqKey="Batejat C" first="Christophe" last="Batéjat">Christophe Batéjat</name></author><author><name sortKey="Burguiere, Ana M" sort="Burguiere, Ana M" uniqKey="Burguiere A" first="Ana M" last="Burguière">Ana M. Burguière</name></author><author><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name></author></analytic><series><title level="j">Influenza and other respiratory viruses</title><idno type="eISSN">1750-2659</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus Infections (virology)</term><term>Disinfection (methods)</term><term>Hot Temperature</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie)</term><term>Désinfection ()</term><term>Humains</term><term>Infections à coronavirus (virologie)</term><term>Température élevée</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Disinfection</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hot Temperature</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Désinfection</term><term>Humains</term><term>Température élevée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log10 . Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25074677</PMID><DateCompleted><Year>2015</Year><Month>05</Month><Day>18</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1750-2659</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>5</Issue><PubDate><Year>2014</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Influenza and other respiratory viruses</Title><ISOAbbreviation>Influenza Other Respir Viruses</ISOAbbreviation></Journal><ArticleTitle>Heat inactivation of the Middle East respiratory syndrome coronavirus.</ArticleTitle><Pagination><MedlinePgn>585-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/irv.12261</ELocationID><Abstract><AbstractText>The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log10 . Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.</AbstractText><CopyrightInformation>© 2014 The Authors. 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Burguière</name><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Leclercq, India" sort="Leclercq, India" uniqKey="Leclercq I" first="India" last="Leclercq">India Leclercq</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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