Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.
Identifieur interne : 001577 ( PubMed/Checkpoint ); précédent : 001576; suivant : 001578Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.
Auteurs : Daesub Song [Corée du Sud] ; Gunwoo Ha [Corée du Sud] ; Wissam Serhan [Émirats arabes unis] ; Yassir Eltahir [Émirats arabes unis] ; Mohammed Yusof [Émirats arabes unis] ; Farouq Hashem [Émirats arabes unis] ; Elsaeid Elsayed [Émirats arabes unis] ; Bahaaeldin Marzoug [Émirats arabes unis] ; Assem Abdelazim [Émirats arabes unis] ; Salama Al Muhairi [Émirats arabes unis]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Antigènes viraux (analyse), Chameaux (virologie), Chromatographie d'affinité (), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Fosse nasale (virologie), Infections à coronavirus (diagnostic), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (virologie), Reproductibilité des résultats, Sensibilité et spécificité, Virologie.
- MESH :
- analyse : Antigènes viraux.
- diagnostic : Infections à coronavirus.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- médecine vétérinaire : Infections à coronavirus.
- virologie : Chameaux, Fosse nasale, Infections à coronavirus.
- Animaux, Chromatographie d'affinité, Reproductibilité des résultats, Sensibilité et spécificité, Virologie.
English descriptors
- KwdEn :
- Animals, Antigens, Viral (analysis), Camelus (virology), Chromatography, Affinity (methods), Coronavirus Infections (diagnosis), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Nasal Cavity (virology), Reproducibility of Results, Sensitivity and Specificity, Virology.
- MESH :
- chemical , analysis : Antigens, Viral.
- diagnosis : Coronavirus Infections.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Chromatography, Affinity.
- veterinary : Coronavirus Infections.
- virology : Camelus, Coronavirus Infections, Nasal Cavity.
- Animals, Reproducibility of Results, Sensitivity and Specificity, Virology.
Abstract
We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.
DOI: 10.1128/JCM.03096-14
PubMed: 25631809
Affiliations:
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pubmed:25631809Le document en format XML
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<front><div type="abstract" xml:lang="en">We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels. </div>
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<Abstract><AbstractText>We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels. </AbstractText>
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