Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.
Identifieur interne : 001714 ( PubMed/Curation ); précédent : 001713; suivant : 001715Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.
Auteurs : Daesub Song [Corée du Sud] ; Gunwoo Ha [Corée du Sud] ; Wissam Serhan [Émirats arabes unis] ; Yassir Eltahir [Émirats arabes unis] ; Mohammed Yusof [Émirats arabes unis] ; Farouq Hashem [Émirats arabes unis] ; Elsaeid Elsayed [Émirats arabes unis] ; Bahaaeldin Marzoug [Émirats arabes unis] ; Assem Abdelazim [Émirats arabes unis] ; Salama Al Muhairi [Émirats arabes unis]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Antigènes viraux (analyse), Chameaux (virologie), Chromatographie d'affinité (), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Fosse nasale (virologie), Infections à coronavirus (diagnostic), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (virologie), Reproductibilité des résultats, Sensibilité et spécificité, Virologie.
- MESH :
- analyse : Antigènes viraux.
- diagnostic : Infections à coronavirus.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- médecine vétérinaire : Infections à coronavirus.
- virologie : Chameaux, Fosse nasale, Infections à coronavirus.
- Animaux, Chromatographie d'affinité, Reproductibilité des résultats, Sensibilité et spécificité, Virologie.
English descriptors
- KwdEn :
- Animals, Antigens, Viral (analysis), Camelus (virology), Chromatography, Affinity (methods), Coronavirus Infections (diagnosis), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Nasal Cavity (virology), Reproducibility of Results, Sensitivity and Specificity, Virology.
- MESH :
- chemical , analysis : Antigens, Viral.
- diagnosis : Coronavirus Infections.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Chromatography, Affinity.
- veterinary : Coronavirus Infections.
- virology : Camelus, Coronavirus Infections, Nasal Cavity.
- Animals, Reproducibility of Results, Sensitivity and Specificity, Virology.
Abstract
We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.
DOI: 10.1128/JCM.03096-14
PubMed: 25631809
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first="Bahaaeldin" last="Marzoug">Bahaaeldin Marzoug</name><affiliation wicri:level="1"><nlm:affiliation>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</nlm:affiliation><country xml:lang="fr">Émirats arabes unis</country><wicri:regionArea>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi</wicri:regionArea></affiliation></author><author><name sortKey="Abdelazim, Assem" sort="Abdelazim, Assem" uniqKey="Abdelazim A" first="Assem" last="Abdelazim">Assem Abdelazim</name><affiliation wicri:level="1"><nlm:affiliation>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</nlm:affiliation><country xml:lang="fr">Émirats arabes unis</country><wicri:regionArea>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi</wicri:regionArea></affiliation></author><author><name sortKey="Al Muhairi, Salama" sort="Al Muhairi, Salama" uniqKey="Al Muhairi S" first="Salama" last="Al Muhairi">Salama Al Muhairi</name><affiliation wicri:level="1"><nlm:affiliation>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates salama.almuhairi@adfca.ae.</nlm:affiliation><country wicri:rule="url">Émirats arabes unis</country><wicri:regionArea>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Viral (analysis)</term><term>Camelus (virology)</term><term>Chromatography, Affinity (methods)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (veterinary)</term><term>Coronavirus Infections (virology)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Nasal Cavity (virology)</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Virology</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigènes viraux (analyse)</term><term>Chameaux (virologie)</term><term>Chromatographie d'affinité ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Fosse nasale (virologie)</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Infections à coronavirus (virologie)</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Virologie</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Affinity</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chameaux</term><term>Fosse nasale</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Camelus</term><term>Coronavirus Infections</term><term>Nasal Cavity</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Virology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chromatographie d'affinité</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Virologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25631809</PMID><DateCompleted><Year>2016</Year><Month>02</Month><Day>10</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-660X</ISSN><JournalIssue CitedMedium="Internet"><Volume>53</Volume><Issue>4</Issue><PubDate><Year>2015</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.</ArticleTitle><Pagination><MedlinePgn>1178-82</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/JCM.03096-14</ELocationID><Abstract><AbstractText>We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels. </AbstractText><CopyrightInformation>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Daesub</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Yuseong-gu, Daejeon, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ha</LastName><ForeName>Gunwoo</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>BioNote, Inc., Hwaseong-si, Gyeonggi-do, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Serhan</LastName><ForeName>Wissam</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eltahir</LastName><ForeName>Yassir</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Epidemiology Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yusof</LastName><ForeName>Mohammed</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hashem</LastName><ForeName>Farouq</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Elsayed</LastName><ForeName>Elsaeid</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marzoug</LastName><ForeName>Bahaaeldin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abdelazim</LastName><ForeName>Assem</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Al Muhairi</LastName><ForeName>Salama</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates salama.almuhairi@adfca.ae.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>01</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002162" MajorTopicYN="N">Camelus</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002846" MajorTopicYN="N">Chromatography, Affinity</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009296" 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