Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC.
Identifieur interne : 000471 ( PubMed/Checkpoint ); précédent : 000470; suivant : 000472Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC.
Auteurs : Kazuya Shirato [Japon] ; Simenew Keskes Melaku [Éthiopie] ; Kengo Kawachi [Japon] ; Naganori Nao [Japon] ; Naoko Iwata-Yoshikawa [Japon] ; Miyuki Kawase [Japon] ; Wataru Kamitani [Japon] ; Shutoku Matsuyama [Japon] ; Tesfaye Sisay Tessema [Éthiopie] ; Hiroshi Sentsui [Japon]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2019.
Abstract
Middle East respiratory syndrome (MERS) is an emerging respiratory disease caused by the MERS coronavirus (MERS-CoV). MERS has been endemic to Saudi Arabia since 2012. The reservoir of MERS-CoV is the dromedary camel, suggesting that MERS is primarily a zoonotic disease. MERS-CoV is common in dromedaries throughout the Middle East, North Africa, and East Africa as evidenced by neutralizing antibodies against MERS-CoV; however, human cases have remained limited to the Middle East. To better understand the cause of this difference, the virological properties of African camel MERS-CoV were analyzed based on the spike (S) protein in Ethiopia. Nasal swabs were collected from 258 young dromedaries (≤ 2 years old) in the Afar region of Ethiopia, of which 39 were positive for MERS-CoV, as confirmed by genetic tests. All positive tests were exclusive to the Amibara woreda region. Using next-generation sequencing, two full-length genomes of Amibara isolates were successfully decoded; both isolates belonged to the C2 clade based on phylogenetic analysis of full-length and S protein sequences. Recombinant EMC isolates of MERS-CoV, in which the S protein is replaced with those of Amibara isolates, were then generated to test the roles of these proteins in viral properties. Amibara S recombinants replicated more slowly in cultured cells than in EMC S recombinants. In neutralizing assays, Amibara S recombinants were neutralized by lower concentrations of sera from both Ethiopian dromedaries and EMC isolate (wild-type)-immunized mouse sera, relative to the EMC S recombinants, indicating that viruses coated in the Amibara S protein were easier to neutralize than the EMC S protein. Neutralization experiments performed using S1/S2 chimeric recombinants of the EMC and Amibara S proteins showed that the neutralization profile was dependent on the S1 region of the S protein. These results suggest that the slower viral replication and the ease of neutralization seen in the Ethiopian MERS-CoV are due to strain-specific differences in the S protein and may account for the absence of human MERS-CoV cases in Ethiopia.
DOI: 10.3389/fmicb.2019.01326
PubMed: 31275264
Affiliations:
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<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome (MERS) is an emerging respiratory disease caused by the MERS coronavirus (MERS-CoV). MERS has been endemic to Saudi Arabia since 2012. The reservoir of MERS-CoV is the dromedary camel, suggesting that MERS is primarily a zoonotic disease. MERS-CoV is common in dromedaries throughout the Middle East, North Africa, and East Africa as evidenced by neutralizing antibodies against MERS-CoV; however, human cases have remained limited to the Middle East. To better understand the cause of this difference, the virological properties of African camel MERS-CoV were analyzed based on the spike (S) protein in Ethiopia. Nasal swabs were collected from 258 young dromedaries (≤ 2 years old) in the Afar region of Ethiopia, of which 39 were positive for MERS-CoV, as confirmed by genetic tests. All positive tests were exclusive to the Amibara woreda region. Using next-generation sequencing, two full-length genomes of Amibara isolates were successfully decoded; both isolates belonged to the C2 clade based on phylogenetic analysis of full-length and S protein sequences. Recombinant EMC isolates of MERS-CoV, in which the S protein is replaced with those of Amibara isolates, were then generated to test the roles of these proteins in viral properties. Amibara S recombinants replicated more slowly in cultured cells than in EMC S recombinants. In neutralizing assays, Amibara S recombinants were neutralized by lower concentrations of sera from both Ethiopian dromedaries and EMC isolate (wild-type)-immunized mouse sera, relative to the EMC S recombinants, indicating that viruses coated in the Amibara S protein were easier to neutralize than the EMC S protein. Neutralization experiments performed using S1/S2 chimeric recombinants of the EMC and Amibara S proteins showed that the neutralization profile was dependent on the S1 region of the S protein. These results suggest that the slower viral replication and the ease of neutralization seen in the Ethiopian MERS-CoV are due to strain-specific differences in the S protein and may account for the absence of human MERS-CoV cases in Ethiopia.</div>
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<Abstract><AbstractText>Middle East respiratory syndrome (MERS) is an emerging respiratory disease caused by the MERS coronavirus (MERS-CoV). MERS has been endemic to Saudi Arabia since 2012. The reservoir of MERS-CoV is the dromedary camel, suggesting that MERS is primarily a zoonotic disease. MERS-CoV is common in dromedaries throughout the Middle East, North Africa, and East Africa as evidenced by neutralizing antibodies against MERS-CoV; however, human cases have remained limited to the Middle East. To better understand the cause of this difference, the virological properties of African camel MERS-CoV were analyzed based on the spike (S) protein in Ethiopia. Nasal swabs were collected from 258 young dromedaries (≤ 2 years old) in the Afar region of Ethiopia, of which 39 were positive for MERS-CoV, as confirmed by genetic tests. All positive tests were exclusive to the Amibara woreda region. Using next-generation sequencing, two full-length genomes of Amibara isolates were successfully decoded; both isolates belonged to the C2 clade based on phylogenetic analysis of full-length and S protein sequences. Recombinant EMC isolates of MERS-CoV, in which the S protein is replaced with those of Amibara isolates, were then generated to test the roles of these proteins in viral properties. Amibara S recombinants replicated more slowly in cultured cells than in EMC S recombinants. In neutralizing assays, Amibara S recombinants were neutralized by lower concentrations of sera from both Ethiopian dromedaries and EMC isolate (wild-type)-immunized mouse sera, relative to the EMC S recombinants, indicating that viruses coated in the Amibara S protein were easier to neutralize than the EMC S protein. Neutralization experiments performed using S1/S2 chimeric recombinants of the EMC and Amibara S proteins showed that the neutralization profile was dependent on the S1 region of the S protein. These results suggest that the slower viral replication and the ease of neutralization seen in the Ethiopian MERS-CoV are due to strain-specific differences in the S protein and may account for the absence of human MERS-CoV cases in Ethiopia.</AbstractText>
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<ReferenceList><Reference><Citation>J Clin Microbiol. 2002 Feb;40(2):372-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11825944</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12543-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16116101</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virus Res. 1991 Mar;18(2-3):99-107</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1645909</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiol Immunol. 2008 Feb;52(2):118-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18380809</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2008 Sep;82(17):8942-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18562527</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2009 Jan;83(2):712-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18971274</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2009 Aug;83(15):7411-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19439480</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biometrika. 1947;34(1-2):28-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20287819</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2012 Jun;86(12):6537-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22496216</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2012 Apr;4(4):557-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22590686</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2012 Sep 27;17(39):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23041020</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2012 Sep 27;17(39):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23041021</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2012 Dec 06;17(49):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23231891</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 May;87(10):5502-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23468491</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Jul;87(14):7790-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23678167</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2013 May;19(5):736-42B</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23693015</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 Aug 1;369(5):407-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23782161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Aug;87(16):9379-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23785207</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Aug 8;500(7461):227-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23831647</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2013 Aug;23(8):986-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23835475</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Oct;87(19):10777-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23903833</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2013 Aug 16;341(6147):702</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23950504</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Dec;87(23):12552-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24027332</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013 Dec 12;18(50):20659</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24342517</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2014 Feb;14(2):140-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24355866</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2014 Feb 18;5(1):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24549846</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2014 Feb 25;5(2):e00884-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24570370</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2014 Jun 26;370(26):2499-505</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24896817</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2014 Jun 12;19(23):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24957744</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2014 Aug;20(8):1370-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25062254</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2014 Dec;20(12):2093-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25425139</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2015 May;15(5):559-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25863564</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2015 Jun;21(6):1019-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25989145</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Jpn J Infect Dis. 2015;68(3):256-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25993975</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2015 Aug 18;5:13133</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26281793</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2015 Nov;25(11):1237-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26391698</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2015;20(37):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26536463</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2016 Jul;33(7):1870-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27004904</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2016 Jun;22(6):1086-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27071076</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2016 Dec 16;91(1):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27733646</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12262-12267</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27791014</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2017 Mar 30;22(13):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28382915</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Microbes Infect. 2017 May 24;6(5):e37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28536429</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2017 Nov;511:95-105</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28843094</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol Methods. 2018 Jan;251:22-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28993122</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):3144-3149</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29507189</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2018 Apr 27;92(10):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29514901</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol Methods. 2018 Aug;258:41-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29763640</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Zoonoses Public Health. 2018 May 31;:</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29855166</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virus Res. 2018 Aug 15;255:14-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29936068</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1985 Dec;56(3):904-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2999443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Rep. 2018 Jul 10;24(2):441-452</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29996104</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2018 Sep 12;92(19):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30021905</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Microbes Infect. 2018 Nov 28;7(1):195</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30482895</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 1988 Dec;69 ( Pt 12):2939-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3058868</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2019 Mar 5;93(6):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30626685</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 1993 Jul;74 ( Pt 7):1421-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7687650</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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