Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia
Identifieur interne : 000754 ( Pmc/Curation ); précédent : 000753; suivant : 000755Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia
Auteurs : Thomas Briese ; Nischay Mishra ; Komal Jain ; Iyad S. Zalmout ; Omar J. Jabado ; William B. Karesh ; Peter Daszak ; Osama B. Mohammed ; Abdulaziz N. Alagaili ; W. Ian LipkinSource :
- mBio [ 2150-7511 ] ; 2014.
Abstract
Complete Middle East respiratory syndrome coronavirus (MERS-CoV) genome sequences were obtained from nasal swabs of dromedary camels sampled in the Kingdom of Saudi Arabia through direct analysis of nucleic acid extracts or following virus isolation in cell culture. Consensus dromedary MERS-CoV genome sequences were the same with either template source and identical to published human MERS-CoV sequences. However, in contrast to individual human cases, where only clonal genomic sequences are reported, detailed population analyses revealed the presence of more than one genomic variant in individual dromedaries. If humans are truly infected only with clonal virus populations, we must entertain a model for interspecies transmission of MERS-CoV wherein only specific genotypes are capable of passing bottleneck selection.
Url:
DOI: 10.1128/mBio.01146-14
PubMed: 24781747
PubMed Central: 4010836
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000754
Links to Exploration step
PMC:4010836Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia</title>
<author><name sortKey="Briese, Thomas" sort="Briese, Thomas" uniqKey="Briese T" first="Thomas" last="Briese">Thomas Briese</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mishra, Nischay" sort="Mishra, Nischay" uniqKey="Mishra N" first="Nischay" last="Mishra">Nischay Mishra</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jain, Komal" sort="Jain, Komal" uniqKey="Jain K" first="Komal" last="Jain">Komal Jain</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zalmout, Iyad S" sort="Zalmout, Iyad S" uniqKey="Zalmout I" first="Iyad S." last="Zalmout">Iyad S. Zalmout</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jabado, Omar J" sort="Jabado, Omar J" uniqKey="Jabado O" first="Omar J." last="Jabado">Omar J. Jabado</name>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Karesh, William B" sort="Karesh, William B" uniqKey="Karesh W" first="William B." last="Karesh">William B. Karesh</name>
<affiliation><nlm:aff id="aff4"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Daszak, Peter" sort="Daszak, Peter" uniqKey="Daszak P" first="Peter" last="Daszak">Peter Daszak</name>
<affiliation><nlm:aff id="aff4"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mohammed, Osama B" sort="Mohammed, Osama B" uniqKey="Mohammed O" first="Osama B." last="Mohammed">Osama B. Mohammed</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Alagaili, Abdulaziz N" sort="Alagaili, Abdulaziz N" uniqKey="Alagaili A" first="Abdulaziz N." last="Alagaili">Abdulaziz N. Alagaili</name>
</author>
<author><name sortKey="Lipkin, W Ian" sort="Lipkin, W Ian" uniqKey="Lipkin W" first="W. Ian" last="Lipkin">W. Ian Lipkin</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24781747</idno>
<idno type="pmc">4010836</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4010836</idno>
<idno type="RBID">PMC:4010836</idno>
<idno type="doi">10.1128/mBio.01146-14</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000754</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000754</idno>
<idno type="wicri:Area/Pmc/Curation">000754</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000754</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia</title>
<author><name sortKey="Briese, Thomas" sort="Briese, Thomas" uniqKey="Briese T" first="Thomas" last="Briese">Thomas Briese</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mishra, Nischay" sort="Mishra, Nischay" uniqKey="Mishra N" first="Nischay" last="Mishra">Nischay Mishra</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jain, Komal" sort="Jain, Komal" uniqKey="Jain K" first="Komal" last="Jain">Komal Jain</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zalmout, Iyad S" sort="Zalmout, Iyad S" uniqKey="Zalmout I" first="Iyad S." last="Zalmout">Iyad S. Zalmout</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jabado, Omar J" sort="Jabado, Omar J" uniqKey="Jabado O" first="Omar J." last="Jabado">Omar J. Jabado</name>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Karesh, William B" sort="Karesh, William B" uniqKey="Karesh W" first="William B." last="Karesh">William B. Karesh</name>
<affiliation><nlm:aff id="aff4"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Daszak, Peter" sort="Daszak, Peter" uniqKey="Daszak P" first="Peter" last="Daszak">Peter Daszak</name>
<affiliation><nlm:aff id="aff4"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mohammed, Osama B" sort="Mohammed, Osama B" uniqKey="Mohammed O" first="Osama B." last="Mohammed">Osama B. Mohammed</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Alagaili, Abdulaziz N" sort="Alagaili, Abdulaziz N" uniqKey="Alagaili A" first="Abdulaziz N." last="Alagaili">Abdulaziz N. Alagaili</name>
</author>
<author><name sortKey="Lipkin, W Ian" sort="Lipkin, W Ian" uniqKey="Lipkin W" first="W. Ian" last="Lipkin">W. Ian Lipkin</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">mBio</title>
<idno type="eISSN">2150-7511</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>Complete Middle East respiratory syndrome coronavirus (MERS-CoV) genome sequences were obtained from nasal swabs of dromedary camels sampled in the Kingdom of Saudi Arabia through direct analysis of nucleic acid extracts or following virus isolation in cell culture. Consensus dromedary MERS-CoV genome sequences were the same with either template source and identical to published human MERS-CoV sequences. However, in contrast to individual human cases, where only clonal genomic sequences are reported, detailed population analyses revealed the presence of more than one genomic variant in individual dromedaries. If humans are truly infected only with clonal virus populations, we must entertain a model for interspecies transmission of MERS-CoV wherein only specific genotypes are capable of passing bottleneck selection.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name>
</author>
<author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name>
</author>
<author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Müller</name>
</author>
<author><name sortKey="Gutierrez, C" uniqKey="Gutierrez C">C Gutierrez</name>
</author>
<author><name sortKey="Godeke, Gj" uniqKey="Godeke G">GJ Godeke</name>
</author>
<author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name>
</author>
<author><name sortKey="Muth, D" uniqKey="Muth D">D Muth</name>
</author>
<author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name>
</author>
<author><name sortKey="Smits De Vries, L" uniqKey="Smits De Vries L">L Smits-De Vries</name>
</author>
<author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
<author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name>
</author>
<author><name sortKey="Smits, Sl" uniqKey="Smits S">SL Smits</name>
</author>
<author><name sortKey="El Tahir, Ye" uniqKey="El Tahir Y">YE El Tahir</name>
</author>
<author><name sortKey="De Sousa, R" uniqKey="De Sousa R">R De Sousa</name>
</author>
<author><name sortKey="Van Beek, J" uniqKey="Van Beek J">J van Beek</name>
</author>
<author><name sortKey="Nowotny, N" uniqKey="Nowotny N">N Nowotny</name>
</author>
<author><name sortKey="Van Maanen, K" uniqKey="Van Maanen K">K van Maanen</name>
</author>
<author><name sortKey="Hidalgo Hermoso, E" uniqKey="Hidalgo Hermoso E">E Hidalgo-Hermoso</name>
</author>
<author><name sortKey="Bosch, Bj" uniqKey="Bosch B">BJ Bosch</name>
</author>
<author><name sortKey="Rottier, P" uniqKey="Rottier P">P Rottier</name>
</author>
<author><name sortKey="Osterhaus, A" uniqKey="Osterhaus A">A Osterhaus</name>
</author>
<author><name sortKey="Gortazar Schmidt, C" uniqKey="Gortazar Schmidt C">C Gortázar-Schmidt</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Koopmans, Mp" uniqKey="Koopmans M">MP Koopmans</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Perera, Ra" uniqKey="Perera R">RA Perera</name>
</author>
<author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name>
</author>
<author><name sortKey="Gomaa, Mr" uniqKey="Gomaa M">MR Gomaa</name>
</author>
<author><name sortKey="El Shesheny, R" uniqKey="El Shesheny R">R El-Shesheny</name>
</author>
<author><name sortKey="Kandeil, A" uniqKey="Kandeil A">A Kandeil</name>
</author>
<author><name sortKey="Bagato, O" uniqKey="Bagato O">O Bagato</name>
</author>
<author><name sortKey="Siu, Ly" uniqKey="Siu L">LY Siu</name>
</author>
<author><name sortKey="Shehata, Mm" uniqKey="Shehata M">MM Shehata</name>
</author>
<author><name sortKey="Kayed, As" uniqKey="Kayed A">AS Kayed</name>
</author>
<author><name sortKey="Moatasim, Y" uniqKey="Moatasim Y">Y Moatasim</name>
</author>
<author><name sortKey="Li, M" uniqKey="Li M">M Li</name>
</author>
<author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
<author><name sortKey="Webby, Rj" uniqKey="Webby R">RJ Webby</name>
</author>
<author><name sortKey="Ali, Ma" uniqKey="Ali M">MA Ali</name>
</author>
<author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name>
</author>
<author><name sortKey="Kayali, G" uniqKey="Kayali G">G Kayali</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hemida, Mg" uniqKey="Hemida M">MG Hemida</name>
</author>
<author><name sortKey="Perera, Ra" uniqKey="Perera R">RA Perera</name>
</author>
<author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name>
</author>
<author><name sortKey="Alhammadi, Ma" uniqKey="Alhammadi M">MA Alhammadi</name>
</author>
<author><name sortKey="Siu, Ly" uniqKey="Siu L">LY Siu</name>
</author>
<author><name sortKey="Li, M" uniqKey="Li M">M Li</name>
</author>
<author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name>
</author>
<author><name sortKey="Saif, L" uniqKey="Saif L">L Saif</name>
</author>
<author><name sortKey="Alnaeem, A" uniqKey="Alnaeem A">A Alnaeem</name>
</author>
<author><name sortKey="Peiris, M" uniqKey="Peiris M">M Peiris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reusken, C" uniqKey="Reusken C">C Reusken</name>
</author>
<author><name sortKey="Ababneh, M" uniqKey="Ababneh M">M Ababneh</name>
</author>
<author><name sortKey="Raj, V" uniqKey="Raj V">V Raj</name>
</author>
<author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name>
</author>
<author><name sortKey="Eljarah, A" uniqKey="Eljarah A">A Eljarah</name>
</author>
<author><name sortKey="Abutarbush, S" uniqKey="Abutarbush S">S Abutarbush</name>
</author>
<author><name sortKey="Godeke, G" uniqKey="Godeke G">G Godeke</name>
</author>
<author><name sortKey="Bestebroer, T" uniqKey="Bestebroer T">T Bestebroer</name>
</author>
<author><name sortKey="Zutt, I" uniqKey="Zutt I">I Zutt</name>
</author>
<author><name sortKey="Muller, M" uniqKey="Muller M">M Muller</name>
</author>
<author><name sortKey="Bosch, B" uniqKey="Bosch B">B Bosch</name>
</author>
<author><name sortKey="Rottier, P" uniqKey="Rottier P">P Rottier</name>
</author>
<author><name sortKey="Osterhaus, A" uniqKey="Osterhaus A">A Osterhaus</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Haagmans, B" uniqKey="Haagmans B">B Haagmans</name>
</author>
<author><name sortKey="Koopmans, M" uniqKey="Koopmans M">M Koopmans</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name>
</author>
<author><name sortKey="Al Dhahiry, Sh" uniqKey="Al Dhahiry S">SH Al Dhahiry</name>
</author>
<author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name>
</author>
<author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name>
</author>
<author><name sortKey="Galiano, M" uniqKey="Galiano M">M Galiano</name>
</author>
<author><name sortKey="Myers, R" uniqKey="Myers R">R Myers</name>
</author>
<author><name sortKey="Godeke, Gj" uniqKey="Godeke G">GJ Godeke</name>
</author>
<author><name sortKey="Jonges, M" uniqKey="Jonges M">M Jonges</name>
</author>
<author><name sortKey="Farag, E" uniqKey="Farag E">E Farag</name>
</author>
<author><name sortKey="Diab, A" uniqKey="Diab A">A Diab</name>
</author>
<author><name sortKey="Ghobashy, H" uniqKey="Ghobashy H">H Ghobashy</name>
</author>
<author><name sortKey="Alhajri, F" uniqKey="Alhajri F">F Alhajri</name>
</author>
<author><name sortKey="Al Thani, M" uniqKey="Al Thani M">M Al-Thani</name>
</author>
<author><name sortKey="Al Marri, Sa" uniqKey="Al Marri S">SA Al-Marri</name>
</author>
<author><name sortKey="Al Romaihi, He" uniqKey="Al Romaihi H">HE Al Romaihi</name>
</author>
<author><name sortKey="Al Khal, A" uniqKey="Al Khal A">A Al Khal</name>
</author>
<author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A Bermingham</name>
</author>
<author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name>
</author>
<author><name sortKey="Alhajri, Mm" uniqKey="Alhajri M">MM AlHajri</name>
</author>
<author><name sortKey="Koopmans, Mp" uniqKey="Koopmans M">MP Koopmans</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alexandersen, S" uniqKey="Alexandersen S">S Alexandersen</name>
</author>
<author><name sortKey="Kobinger, Gp" uniqKey="Kobinger G">GP Kobinger</name>
</author>
<author><name sortKey="Soule, G" uniqKey="Soule G">G Soule</name>
</author>
<author><name sortKey="Wernery, U" uniqKey="Wernery U">U Wernery</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name>
</author>
<author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Müller</name>
</author>
<author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
<author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name>
</author>
<author><name sortKey="Ritz, D" uniqKey="Ritz D">D Ritz</name>
</author>
<author><name sortKey="Godeke, Gj" uniqKey="Godeke G">GJ Godeke</name>
</author>
<author><name sortKey="Lattwein, E" uniqKey="Lattwein E">E Lattwein</name>
</author>
<author><name sortKey="Kallies, S" uniqKey="Kallies S">S Kallies</name>
</author>
<author><name sortKey="Siemens, A" uniqKey="Siemens A">A Siemens</name>
</author>
<author><name sortKey="Van Beek, J" uniqKey="Van Beek J">J van Beek</name>
</author>
<author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name>
</author>
<author><name sortKey="Muth, D" uniqKey="Muth D">D Muth</name>
</author>
<author><name sortKey="Bosch, Bj" uniqKey="Bosch B">BJ Bosch</name>
</author>
<author><name sortKey="Wernery, U" uniqKey="Wernery U">U Wernery</name>
</author>
<author><name sortKey="Koopmans, Mp" uniqKey="Koopmans M">MP Koopmans</name>
</author>
<author><name sortKey="Wernery, R" uniqKey="Wernery R">R Wernery</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alagaili, An" uniqKey="Alagaili A">AN Alagaili</name>
</author>
<author><name sortKey="Briese, T" uniqKey="Briese T">T Briese</name>
</author>
<author><name sortKey="Mishra, N" uniqKey="Mishra N">N Mishra</name>
</author>
<author><name sortKey="Kapoor, V" uniqKey="Kapoor V">V Kapoor</name>
</author>
<author><name sortKey="Sameroff, Sc" uniqKey="Sameroff S">SC Sameroff</name>
</author>
<author><name sortKey="Burbelo, Pd" uniqKey="Burbelo P">PD Burbelo</name>
</author>
<author><name sortKey="De Wit, E" uniqKey="De Wit E">E de Wit</name>
</author>
<author><name sortKey="Munster, Vj" uniqKey="Munster V">VJ Munster</name>
</author>
<author><name sortKey="Hensley, Le" uniqKey="Hensley L">LE Hensley</name>
</author>
<author><name sortKey="Zalmout, Is" uniqKey="Zalmout I">IS Zalmout</name>
</author>
<author><name sortKey="Kapoor, A" uniqKey="Kapoor A">A Kapoor</name>
</author>
<author><name sortKey="Epstein, Jh" uniqKey="Epstein J">JH Epstein</name>
</author>
<author><name sortKey="Karesh, Wb" uniqKey="Karesh W">WB Karesh</name>
</author>
<author><name sortKey="Daszak, P" uniqKey="Daszak P">P Daszak</name>
</author>
<author><name sortKey="Mohammed, Ob" uniqKey="Mohammed O">OB Mohammed</name>
</author>
<author><name sortKey="Lipkin, Wi" uniqKey="Lipkin W">WI Lipkin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Memish, Za" uniqKey="Memish Z">ZA Memish</name>
</author>
<author><name sortKey="Cotton, M" uniqKey="Cotton M">M Cotton</name>
</author>
<author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name>
</author>
<author><name sortKey="Watson, Sj" uniqKey="Watson S">SJ Watson</name>
</author>
<author><name sortKey="Alsahafi, Aj" uniqKey="Alsahafi A">AJ Alsahafi</name>
</author>
<author><name sortKey="Al Rabeeah, Aa" uniqKey="Al Rabeeah A">AA Al Rabeeah</name>
</author>
<author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
<author><name sortKey="Sieberg, A" uniqKey="Sieberg A">A Sieberg</name>
</author>
<author><name sortKey="Makhdoom, Hq" uniqKey="Makhdoom H">HQ Makhdoom</name>
</author>
<author><name sortKey="Assiri, A" uniqKey="Assiri A">A Assiri</name>
</author>
<author><name sortKey="Al Masri, M" uniqKey="Al Masri M">M Al Masri</name>
</author>
<author><name sortKey="Bosch, Bj" uniqKey="Bosch B">BJ Bosch</name>
</author>
<author><name sortKey="Beer, M" uniqKey="Beer M">M Beer</name>
</author>
<author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Müller</name>
</author>
<author><name sortKey="Kellam, P" uniqKey="Kellam P">P Kellam</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chu, Dkw" uniqKey="Chu D">DKW Chu</name>
</author>
<author><name sortKey="Poon, Llm" uniqKey="Poon L">LLM Poon</name>
</author>
<author><name sortKey="Gomaa, Mm" uniqKey="Gomaa M">MM Gomaa</name>
</author>
<author><name sortKey="Shehata, Mm" uniqKey="Shehata M">MM Shehata</name>
</author>
<author><name sortKey="Perera, Rapm" uniqKey="Perera R">RAPM Perera</name>
</author>
<author><name sortKey="Zeid, Da" uniqKey="Zeid D">DA Zeid</name>
</author>
<author><name sortKey="El Rifay, As" uniqKey="El Rifay A">AS El Rifay</name>
</author>
<author><name sortKey="Siu, Ly" uniqKey="Siu L">LY Siu</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
<author><name sortKey="Webby, Rj" uniqKey="Webby R">RJ Webby</name>
</author>
<author><name sortKey="Ali, Ma" uniqKey="Ali M">MA Ali</name>
</author>
<author><name sortKey="Peiris, M" uniqKey="Peiris M">M Peiris</name>
</author>
<author><name sortKey="Kayali, G" uniqKey="Kayali G">G Kayali</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lauring, As" uniqKey="Lauring A">AS Lauring</name>
</author>
<author><name sortKey="Andino, R" uniqKey="Andino R">R Andino</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vignuzzi, M" uniqKey="Vignuzzi M">M Vignuzzi</name>
</author>
<author><name sortKey="Stone, Jk" uniqKey="Stone J">JK Stone</name>
</author>
<author><name sortKey="Arnold, Jj" uniqKey="Arnold J">JJ Arnold</name>
</author>
<author><name sortKey="Cameron, Ce" uniqKey="Cameron C">CE Cameron</name>
</author>
<author><name sortKey="Andino, R" uniqKey="Andino R">R Andino</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cotten, M" uniqKey="Cotten M">M Cotten</name>
</author>
<author><name sortKey="Lam, Tt" uniqKey="Lam T">TT Lam</name>
</author>
<author><name sortKey="Watson, Sj" uniqKey="Watson S">SJ Watson</name>
</author>
<author><name sortKey="Palser, Al" uniqKey="Palser A">AL Palser</name>
</author>
<author><name sortKey="Petrova, V" uniqKey="Petrova V">V Petrova</name>
</author>
<author><name sortKey="Grant, P" uniqKey="Grant P">P Grant</name>
</author>
<author><name sortKey="Pybus, Og" uniqKey="Pybus O">OG Pybus</name>
</author>
<author><name sortKey="Rambaut, A" uniqKey="Rambaut A">A Rambaut</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
<author><name sortKey="Pillay, D" uniqKey="Pillay D">D Pillay</name>
</author>
<author><name sortKey="Kellam, P" uniqKey="Kellam P">P Kellam</name>
</author>
<author><name sortKey="Nastouli, E" uniqKey="Nastouli E">E Nastouli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Langmead, B" uniqKey="Langmead B">B Langmead</name>
</author>
<author><name sortKey="Salzberg, Sl" uniqKey="Salzberg S">SL Salzberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, H" uniqKey="Li H">H Li</name>
</author>
<author><name sortKey="Handsaker, B" uniqKey="Handsaker B">B Handsaker</name>
</author>
<author><name sortKey="Wysoker, A" uniqKey="Wysoker A">A Wysoker</name>
</author>
<author><name sortKey="Fennell, T" uniqKey="Fennell T">T Fennell</name>
</author>
<author><name sortKey="Ruan, J" uniqKey="Ruan J">J Ruan</name>
</author>
<author><name sortKey="Homer, N" uniqKey="Homer N">N Homer</name>
</author>
<author><name sortKey="Marth, G" uniqKey="Marth G">G Marth</name>
</author>
<author><name sortKey="Abecasis, G" uniqKey="Abecasis G">G Abecasis</name>
</author>
<author><name sortKey="Durbin, R" uniqKey="Durbin R">R Durbin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
<author><name sortKey="Eckerle, I" uniqKey="Eckerle I">I Eckerle</name>
</author>
<author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T Bleicker</name>
</author>
<author><name sortKey="Zaki, A" uniqKey="Zaki A">A Zaki</name>
</author>
<author><name sortKey="Landt, O" uniqKey="Landt O">O Landt</name>
</author>
<author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M Eschbach-Bludau</name>
</author>
<author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name>
</author>
<author><name sortKey="Gopal, R" uniqKey="Gopal R">R Gopal</name>
</author>
<author><name sortKey="Ballhause, M" uniqKey="Ballhause M">M Ballhause</name>
</author>
<author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name>
</author>
<author><name sortKey="Muth, D" uniqKey="Muth D">D Muth</name>
</author>
<author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Muller</name>
</author>
<author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name>
</author>
<author><name sortKey="Zambon, M" uniqKey="Zambon M">M Zambon</name>
</author>
<author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name>
</author>
<author><name sortKey="Fouchier, Rm" uniqKey="Fouchier R">RM Fouchier</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name>
</author>
<author><name sortKey="Stecher, G" uniqKey="Stecher G">G Stecher</name>
</author>
<author><name sortKey="Peterson, D" uniqKey="Peterson D">D Peterson</name>
</author>
<author><name sortKey="Filipski, A" uniqKey="Filipski A">A Filipski</name>
</author>
<author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">mBio</journal-id>
<journal-id journal-id-type="iso-abbrev">MBio</journal-id>
<journal-id journal-id-type="hwp">mbio</journal-id>
<journal-id journal-id-type="pmc">mbio</journal-id>
<journal-id journal-id-type="publisher-id">mBio</journal-id>
<journal-title-group><journal-title>mBio</journal-title>
</journal-title-group>
<issn pub-type="epub">2150-7511</issn>
<publisher><publisher-name>American Society of Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24781747</article-id>
<article-id pub-id-type="pmc">4010836</article-id>
<article-id pub-id-type="publisher-id">mBio01146-14</article-id>
<article-id pub-id-type="doi">10.1128/mBio.01146-14</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Observation</subject>
</subj-group>
</article-categories>
<title-group><article-title>Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia</article-title>
<alt-title>Dromedary MERS-CoV Genomic Sequence</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Briese</surname>
<given-names>Thomas</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mishra</surname>
<given-names>Nischay</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jain</surname>
<given-names>Komal</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zalmout</surname>
<given-names>Iyad S.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jabado</surname>
<given-names>Omar J.</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Karesh</surname>
<given-names>William B.</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>d</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Daszak</surname>
<given-names>Peter</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>d</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mohammed</surname>
<given-names>Osama B.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Alagaili</surname>
<given-names>Abdulaziz N.</given-names>
</name>
<xref ref-type="aff" rid="aff2 aff5"><sup>b,e</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lipkin</surname>
<given-names>W. Ian</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<aff id="aff1"><label><sup>a</sup>
</label>
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA</aff>
<aff id="aff2"><label><sup>b</sup>
</label>
KSU Mammals Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia</aff>
<aff id="aff3"><label><sup>c</sup>
</label>
Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, New York, USA</aff>
<aff id="aff4"><label><sup>d</sup>
</label>
EcoHealth Alliance, New York, New York, USA</aff>
<aff id="aff5"><label><sup>e</sup>
</label>
Saudi Wildlife Authority, Riyadh, Saudi Arabia</aff>
</contrib-group>
<author-notes><corresp id="cor1">Address correspondence to Thomas Briese, <email>tb2047@cumc.columbia.edu</email>
, or Abdulaziz N. Alagaili, <email>aziz99@gmail.com</email>
.</corresp>
<fn fn-type="equal"><p>T.B. and N.M. contributed equally to this article.</p>
</fn>
<fn fn-type="edited-by"><p><bold>Editor</bold>
Michael Katze, University of Washington</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>29</day>
<month>4</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="collection"><season>May-Jun</season>
<year>2014</year>
</pub-date>
<volume>5</volume>
<issue>3</issue>
<elocation-id>e01146-14</elocation-id>
<history><date date-type="received"><day>9</day>
<month>4</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>16</day>
<month>4</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014 Briese et al.</copyright-statement>
<copyright-year>2014</copyright-year>
<copyright-holder>Briese et al.</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/"><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/">Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license</ext-link>
, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
</license>
</permissions>
<abstract><title>ABSTRACT</title>
<p>Complete Middle East respiratory syndrome coronavirus (MERS-CoV) genome sequences were obtained from nasal swabs of dromedary camels sampled in the Kingdom of Saudi Arabia through direct analysis of nucleic acid extracts or following virus isolation in cell culture. Consensus dromedary MERS-CoV genome sequences were the same with either template source and identical to published human MERS-CoV sequences. However, in contrast to individual human cases, where only clonal genomic sequences are reported, detailed population analyses revealed the presence of more than one genomic variant in individual dromedaries. If humans are truly infected only with clonal virus populations, we must entertain a model for interspecies transmission of MERS-CoV wherein only specific genotypes are capable of passing bottleneck selection.</p>
</abstract>
<abstract abstract-type="executive-summary"><title>IMPORTANCE</title>
<p>In most cases of Middle East respiratory syndrome (MERS), the route for human infection with the causative agent, MERS coronavirus (MERS-CoV), is unknown. Antibodies to and viral nucleic acids of MERS-CoV have been found in dromedaries, suggesting the possibility that they may serve as a reservoir or vector for human infection. However, neither whole viral genomic sequence nor infectious virus has been isolated from dromedaries or other animals in Saudi Arabia. Here, we report recovery of MERS-CoV from nasal swabs of dromedaries, demonstrate that MERS-CoV whole-genome consensus sequences from dromedaries and humans are indistinguishable, and show that dromedaries can be simultaneously infected with more than one MERS-CoV. Together with data indicating widespread dromedary infection in the Kingdom of Saudi Arabia, these findings support the plausibility of a role for dromedaries in human infection.</p>
</abstract>
<counts><page-count count="5"></page-count>
</counts>
<custom-meta-group><custom-meta><meta-name>cover-date</meta-name>
<meta-value>May/June 2014</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000754 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000754 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:4010836 |texte= Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:24781747" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |