Distribution and absence of generalized lesions in mice following single dose intramuscular inoculation of the vaccine candidate MVA-MERS-S
Identifieur interne : 000662 ( Pmc/Curation ); précédent : 000661; suivant : 000663Distribution and absence of generalized lesions in mice following single dose intramuscular inoculation of the vaccine candidate MVA-MERS-S
Auteurs : Martin C. Langenmayer [Allemagne] ; Anna-Theresa Lülf-Averhoff [Allemagne] ; Silvia Adam-Neumair [Allemagne] ; Robert Fux [Allemagne] ; Gerd Sutter [Allemagne] ; Asisa Volz [Allemagne]Source :
- Biologicals [ 1045-1056 ] ; 2018.
Abstract
Modified Vaccinia Virus Ankara (MVA) is a highly attenuated and replication-deficient virus serving as vaccine against infectious diseases. Here, we assessed the
Url:
DOI: 10.1016/j.biologicals.2018.05.004
PubMed: 29759890
PubMed Central: 7128986
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Langenmayer</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for Infectious Diseases and Zoonoses, LMU Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Infectious Diseases and Zoonoses, LMU Munich</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">German Center for Infection Research (DZIF), Munich Partner Site, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Munich Partner Site</wicri:regionArea></affiliation></author><author><name sortKey="Lulf Averhoff, Anna Theresa" sort="Lulf Averhoff, Anna Theresa" uniqKey="Lulf Averhoff A" first="Anna-Theresa" last="Lülf-Averhoff">Anna-Theresa Lülf-Averhoff</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for Infectious Diseases and Zoonoses, LMU Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Infectious Diseases and Zoonoses, LMU Munich</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">German Center for Infection Research (DZIF), Munich Partner Site, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Munich Partner Site</wicri:regionArea></affiliation></author><author><name sortKey="Adam Neumair, Silvia" sort="Adam Neumair, Silvia" uniqKey="Adam Neumair S" first="Silvia" last="Adam-Neumair">Silvia Adam-Neumair</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for Infectious Diseases and Zoonoses, LMU Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Infectious Diseases and Zoonoses, LMU Munich</wicri:regionArea></affiliation></author><author><name sortKey="Fux, Robert" sort="Fux, Robert" uniqKey="Fux R" first="Robert" last="Fux">Robert Fux</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for Infectious Diseases and Zoonoses, LMU Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Infectious Diseases and Zoonoses, LMU Munich</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">German Center for Infection Research (DZIF), Munich Partner Site, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Munich Partner Site</wicri:regionArea></affiliation></author><author><name sortKey="Sutter, Gerd" sort="Sutter, Gerd" uniqKey="Sutter G" first="Gerd" last="Sutter">Gerd Sutter</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for Infectious Diseases and Zoonoses, LMU Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Infectious Diseases and Zoonoses, LMU Munich</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">German Center for Infection Research (DZIF), Munich Partner Site, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Munich Partner Site</wicri:regionArea></affiliation></author><author><name sortKey="Volz, Asisa" sort="Volz, Asisa" uniqKey="Volz A" first="Asisa" last="Volz">Asisa Volz</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for Infectious Diseases and Zoonoses, LMU Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Infectious Diseases and Zoonoses, LMU Munich</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">German Center for Infection Research (DZIF), Munich Partner Site, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Munich Partner Site</wicri:regionArea></affiliation></author></analytic><series><title level="j">Biologicals</title><idno type="ISSN">1045-1056</idno><idno type="eISSN">1095-8320</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Modified Vaccinia Virus Ankara (MVA) is a highly attenuated and replication-deficient virus serving as vaccine against infectious diseases. Here, we assessed the <italic>in vivo</italic> distribution of a recombinant MVA candidate vaccine against the Middle Eastern Respiratory Syndrome (MVA-MERS-S) in mice. Intramuscularly inoculated mice were necropsied at different time points and examined by histology, immunohistochemistry and real-time PCR. We detected inflammation and myonecrosis at the parenteral site and hyperplasia of the draining lymph nodes. MVA-MERS-S did not result in detectable lesions in tissues peripheral to the parenteral site and draining lymph nodes. Real-time PCR analysis of >240 tissue samples detected MVA-DNA predominantly at the injection site and in the draining lymph nodes, and suggested continuous clearance of the candidate vaccine during the observation period. Levels of parenteral site inflammation and hyperplasia of draining lymph nodes were considered in line with immunological responses to vaccine inoculation.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Mayr, A" uniqKey="Mayr A">A. Mayr</name></author><author><name sortKey="Hochstein Mintzel, V" uniqKey="Hochstein Mintzel V">V. Hochstein-Mintzel</name></author><author><name sortKey="Stickl, H" uniqKey="Stickl H">H. Stickl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Volz, A" uniqKey="Volz A">A. Volz</name></author><author><name sortKey="Sutter, G" uniqKey="Sutter G">G. Sutter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Verheust, C" uniqKey="Verheust C">C. Verheust</name></author><author><name sortKey="Goossens, M" uniqKey="Goossens M">M. Goossens</name></author><author><name sortKey="Pauwels, K" uniqKey="Pauwels K">K. Pauwels</name></author><author><name sortKey="Breyer, D" uniqKey="Breyer D">D. Breyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sutter, G" uniqKey="Sutter G">G. Sutter</name></author><author><name sortKey="Moss, B" uniqKey="Moss B">B. Moss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hanke, T" uniqKey="Hanke T">T. Hanke</name></author><author><name sortKey="Mcmichael, A J" uniqKey="Mcmichael A">A.J. McMichael</name></author><author><name sortKey="Dennis, M J" uniqKey="Dennis M">M.J. Dennis</name></author><author><name sortKey="Sharpe, S A" uniqKey="Sharpe S">S.A. Sharpe</name></author><author><name sortKey="Powell, L A" uniqKey="Powell L">L.A. Powell</name></author><author><name sortKey="Mcloughlin, L" uniqKey="Mcloughlin L">L. McLoughlin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hanke, T" uniqKey="Hanke T">T. Hanke</name></author><author><name sortKey="Mcmichael, A J" uniqKey="Mcmichael A">A.J. McMichael</name></author><author><name sortKey="Samuel, R V" uniqKey="Samuel R">R.V. Samuel</name></author><author><name sortKey="Powell, L A" uniqKey="Powell L">L.A. Powell</name></author><author><name sortKey="Mcloughlin, L" uniqKey="Mcloughlin L">L. McLoughlin</name></author><author><name sortKey="Crome, S J" uniqKey="Crome S">S.J. Crome</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ondondo, B" uniqKey="Ondondo B">B. Ondondo</name></author><author><name sortKey="Brennan, C" uniqKey="Brennan C">C. Brennan</name></author><author><name sortKey="Nicosia, A" uniqKey="Nicosia A">A. Nicosia</name></author><author><name sortKey="Crome, S J" uniqKey="Crome S">S.J. Crome</name></author><author><name sortKey="Hanke, T" uniqKey="Hanke T">T. Hanke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stittelaar, K J" uniqKey="Stittelaar K">K.J. Stittelaar</name></author><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T. Kuiken</name></author><author><name sortKey="De Swart, R L" uniqKey="De Swart R">R.L. de Swart</name></author><author><name sortKey="Van Amerongen, G" uniqKey="Van Amerongen G">G. van Amerongen</name></author><author><name sortKey="Vos, H W" uniqKey="Vos H">H.W. Vos</name></author><author><name sortKey="Niesters, H G" uniqKey="Niesters H">H.G. Niesters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sanchez Sampedro, L" uniqKey="Sanchez Sampedro L">L. Sanchez-Sampedro</name></author><author><name sortKey="Perdiguero, B" uniqKey="Perdiguero B">B. Perdiguero</name></author><author><name sortKey="Mejias Perez, E" uniqKey="Mejias Perez E">E. Mejias-Perez</name></author><author><name sortKey="Garcia Arriaza, J" uniqKey="Garcia Arriaza J">J. Garcia-Arriaza</name></author><author><name sortKey="Di Pilato, M" uniqKey="Di Pilato M">M. Di Pilato</name></author><author><name sortKey="Esteban, M" uniqKey="Esteban M">M. Esteban</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Volz, A" uniqKey="Volz A">A. Volz</name></author><author><name sortKey="Kupke, A" uniqKey="Kupke A">A. Kupke</name></author><author><name sortKey="Song, F" uniqKey="Song F">F. Song</name></author><author><name sortKey="Jany, S" uniqKey="Jany S">S. Jany</name></author><author><name sortKey="Fux, R" uniqKey="Fux R">R. Fux</name></author><author><name sortKey="Shams Eldin, H" uniqKey="Shams Eldin H">H. Shams-Eldin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Van Den Brand, J M" uniqKey="Van Den Brand J">J.M. van den Brand</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Volz, A" uniqKey="Volz A">A. Volz</name></author><author><name sortKey="Wohlsein, P" uniqKey="Wohlsein P">P. Wohlsein</name></author><author><name sortKey="Smits, S L" uniqKey="Smits S">S.L. Smits</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lulf, A T" uniqKey="Lulf A">A.T. Lülf</name></author><author><name sortKey="Freudenstein, A" uniqKey="Freudenstein A">A. Freudenstein</name></author><author><name sortKey="Marr, L" uniqKey="Marr L">L. Marr</name></author><author><name sortKey="Sutter, G" uniqKey="Sutter G">G. Sutter</name></author><author><name sortKey="Volz, A" uniqKey="Volz A">A. Volz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schroeder, K" uniqKey="Schroeder K">K. Schroeder</name></author><author><name sortKey="Nitsche, A" uniqKey="Nitsche A">A. Nitsche</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Volz, A" uniqKey="Volz A">A. Volz</name></author><author><name sortKey="Langenmayer, M C" uniqKey="Langenmayer M">M.C. Langenmayer</name></author><author><name sortKey="Jany, S" uniqKey="Jany S">S. Jany</name></author><author><name sortKey="Kalinke, U" uniqKey="Kalinke U">U. Kalinke</name></author><author><name sortKey="Sutter, G" uniqKey="Sutter G">G. Sutter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valli, V E O" uniqKey="Valli V">V.E.O. Valli</name></author><author><name sortKey="Kiupel, M" uniqKey="Kiupel M">M. Kiupel</name></author><author><name sortKey="Bienzle, D" uniqKey="Bienzle D">D. Bienzle</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biologicals</journal-id><journal-id journal-id-type="iso-abbrev">Biologicals</journal-id><journal-title-group><journal-title>Biologicals</journal-title></journal-title-group><issn pub-type="ppub">1045-1056</issn><issn pub-type="epub">1095-8320</issn><publisher><publisher-name>International Alliance for Biological Standardization. Published by Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">29759890</article-id><article-id pub-id-type="pmc">7128986</article-id><article-id pub-id-type="publisher-id">S1045-1056(18)30137-4</article-id><article-id pub-id-type="doi">10.1016/j.biologicals.2018.05.004</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Distribution and absence of generalized lesions in mice following single dose intramuscular inoculation of the vaccine candidate MVA-MERS-S</article-title></title-group><contrib-group><contrib contrib-type="author" id="au1"><name><surname>Langenmayer</surname><given-names>Martin C.</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au2"><name><surname>Lülf-Averhoff</surname><given-names>Anna-Theresa</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au3"><name><surname>Adam-Neumair</surname><given-names>Silvia</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au4"><name><surname>Fux</surname><given-names>Robert</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au5"><name><surname>Sutter</surname><given-names>Gerd</given-names></name><email>gerd.sutter@lmu.de</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref><xref rid="cor1" ref-type="corresp">∗</xref></contrib><contrib contrib-type="author" id="au6"><name><surname>Volz</surname><given-names>Asisa</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Institute for Infectious Diseases and Zoonoses, LMU Munich, Germany</aff><aff id="aff2"><label>b</label>German Center for Infection Research (DZIF), Munich Partner Site, Germany</aff><author-notes><corresp id="cor1"><label>∗</label>Corresponding author. Institute for Infectious Diseases and Zoonoses, Veterinaerstrasse 13, D-80539 Munich, Germany. <email>gerd.sutter@lmu.de</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>6</day><month>7</month><year>2018</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>7</month><year>2018</year></pub-date><pub-date pub-type="epub"><day>6</day><month>7</month><year>2018</year></pub-date><volume>54</volume><fpage>58</fpage><lpage>62</lpage><history><date date-type="received"><day>13</day><month>3</month><year>2017</year></date><date date-type="rev-recd"><day>14</day><month>3</month><year>2018</year></date><date date-type="accepted"><day>4</day><month>5</month><year>2018</year></date></history><permissions><copyright-statement>© 2018 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2018</copyright-year><copyright-holder>International Alliance for Biological Standardization</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract id="abs0010"><p>Modified Vaccinia Virus Ankara (MVA) is a highly attenuated and replication-deficient virus serving as vaccine against infectious diseases. Here, we assessed the <italic>in vivo</italic> distribution of a recombinant MVA candidate vaccine against the Middle Eastern Respiratory Syndrome (MVA-MERS-S) in mice. Intramuscularly inoculated mice were necropsied at different time points and examined by histology, immunohistochemistry and real-time PCR. We detected inflammation and myonecrosis at the parenteral site and hyperplasia of the draining lymph nodes. MVA-MERS-S did not result in detectable lesions in tissues peripheral to the parenteral site and draining lymph nodes. Real-time PCR analysis of >240 tissue samples detected MVA-DNA predominantly at the injection site and in the draining lymph nodes, and suggested continuous clearance of the candidate vaccine during the observation period. Levels of parenteral site inflammation and hyperplasia of draining lymph nodes were considered in line with immunological responses to vaccine inoculation.</p></abstract><kwd-group id="kwrds0010"><title>Keywords</title><kwd>Viral vector</kwd><kwd>Poxvirus vaccine</kwd><kwd>Vaccinia virus MVA</kwd><kwd>Biodistribution</kwd><kwd>MERS</kwd><kwd>Immunohistochemistry</kwd><kwd>PCR</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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