Functional Dipeptidyl Peptidase 4 (DPP4) in mink supports entry and replication of Middle Eastern respiratory syndrome coronavirus: American Mink (Neovision vision), a novel in vivo model of MERS-CoV infection
Identifieur interne : 000583 ( Pmc/Corpus ); précédent : 000582; suivant : 000584Functional Dipeptidyl Peptidase 4 (DPP4) in mink supports entry and replication of Middle Eastern respiratory syndrome coronavirus: American Mink (Neovision vision), a novel in vivo model of MERS-CoV infection
Auteurs : S. K. Naveen ; C. Kannadka ; M.-C. Chen ; S.-C. Lin ; G. J. N. Nichols ; M. Patterson ; M. Kappes ; T. G. VossSource :
- International Journal of Infectious Diseases [ 1201-9712 ] ; 2016.
Url:
DOI: 10.1016/j.ijid.2016.02.079
PubMed: NONE
PubMed Central: 7128900
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PMC:7128900Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Functional Dipeptidyl Peptidase 4 (DPP4) in mink supports entry and replication of Middle Eastern respiratory syndrome coronavirus: American Mink (Neovision vision), a novel in vivo model of MERS-CoV infection</title><author><name sortKey="Naveen, S K" sort="Naveen, S K" uniqKey="Naveen S" first="S. K." last="Naveen">S. K. Naveen</name><affiliation><nlm:aff id="aff0005">SRI International, Harrisonburg, Virginia, USA</nlm:aff></affiliation></author><author><name sortKey="Kannadka, C" sort="Kannadka, C" uniqKey="Kannadka C" first="C." last="Kannadka">C. Kannadka</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Chen, M C" sort="Chen, M C" uniqKey="Chen M" first="M.-C." last="Chen">M.-C. Chen</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Lin, S C" sort="Lin, S C" uniqKey="Lin S" first="S.-C." last="Lin">S.-C. Lin</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Nichols, G J N" sort="Nichols, G J N" uniqKey="Nichols G" first="G. J. N." last="Nichols">G. J. N. Nichols</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Patterson, M" sort="Patterson, M" uniqKey="Patterson M" first="M." last="Patterson">M. Patterson</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Kappes, M" sort="Kappes, M" uniqKey="Kappes M" first="M." last="Kappes">M. Kappes</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Voss, T G" sort="Voss, T G" uniqKey="Voss T" first="T. G." last="Voss">T. G. Voss</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7128900</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128900</idno><idno type="RBID">PMC:7128900</idno><idno type="doi">10.1016/j.ijid.2016.02.079</idno><idno type="pmid">NONE</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000583</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000583</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Functional Dipeptidyl Peptidase 4 (DPP4) in mink supports entry and replication of Middle Eastern respiratory syndrome coronavirus: American Mink (Neovision vision), a novel in vivo model of MERS-CoV infection</title><author><name sortKey="Naveen, S K" sort="Naveen, S K" uniqKey="Naveen S" first="S. K." last="Naveen">S. K. Naveen</name><affiliation><nlm:aff id="aff0005">SRI International, Harrisonburg, Virginia, USA</nlm:aff></affiliation></author><author><name sortKey="Kannadka, C" sort="Kannadka, C" uniqKey="Kannadka C" first="C." last="Kannadka">C. Kannadka</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Chen, M C" sort="Chen, M C" uniqKey="Chen M" first="M.-C." last="Chen">M.-C. Chen</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Lin, S C" sort="Lin, S C" uniqKey="Lin S" first="S.-C." last="Lin">S.-C. Lin</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Nichols, G J N" sort="Nichols, G J N" uniqKey="Nichols G" first="G. J. N." last="Nichols">G. J. N. Nichols</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Patterson, M" sort="Patterson, M" uniqKey="Patterson M" first="M." last="Patterson">M. Patterson</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Kappes, M" sort="Kappes, M" uniqKey="Kappes M" first="M." last="Kappes">M. Kappes</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author><author><name sortKey="Voss, T G" sort="Voss, T G" uniqKey="Voss T" first="T. G." last="Voss">T. G. Voss</name><affiliation><nlm:aff id="aff0010">SRI International, Harrisonburg, USA</nlm:aff></affiliation></author></analytic><series><title level="j">International Journal of Infectious Diseases</title><idno type="ISSN">1201-9712</idno><idno type="eISSN">1878-3511</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader></TEI><pmc article-type="abstract"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Int J Infect Dis</journal-id><journal-id journal-id-type="iso-abbrev">Int. J. Infect. Dis</journal-id><journal-title-group><journal-title>International Journal of Infectious Diseases</journal-title></journal-title-group><issn pub-type="ppub">1201-9712</issn><issn pub-type="epub">1878-3511</issn><publisher><publisher-name>Published by Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7128900</article-id><article-id pub-id-type="publisher-id">S1201-9712(16)30058-3</article-id><article-id pub-id-type="doi">10.1016/j.ijid.2016.02.079</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Functional Dipeptidyl Peptidase 4 (DPP4) in mink supports entry and replication of Middle Eastern respiratory syndrome coronavirus: American Mink (Neovision vision), a novel in vivo model of MERS-CoV infection</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Naveen</surname><given-names>S.K.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Kannadka</surname><given-names>C.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Chen</surname><given-names>M.-C.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Lin</surname><given-names>S.-C.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0025"><name><surname>Nichols</surname><given-names>G.J.N.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0030"><name><surname>Patterson</surname><given-names>M.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0035"><name><surname>Kappes</surname><given-names>M.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0040"><name><surname>Voss</surname><given-names>T.G.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib></contrib-group><aff id="aff0005"><label>1</label>SRI International, Harrisonburg, Virginia, USA</aff><aff id="aff0010"><label>2</label>SRI International, Harrisonburg, USA</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author.</corresp></author-notes><pub-date pub-type="pmc-release"><day>29</day><month>3</month><year>2016</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>4</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>29</day><month>3</month><year>2016</year></pub-date><volume>45</volume><fpage>21</fpage><lpage>21</lpage><permissions><copyright-statement>Copyright © 2016 Published by Elsevier Ltd.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder></copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions></article-meta></front><body><p id="par0005"><bold>Background</bold>: A novel coronavirus, named the Middle East Respiratory Syndrome coronavirus (MERS-CoV), was first identified in humans in 2012. MERS infections are characterized by acute respiratory distress with fatal cases often diagnosed with comorbidity factors including diabetes, cardiovascular disease, or obesity. Continued emergence of MERS-CoV, coupled with a lack of understanding of the natural history of MERS highlights the importance of identifying therapeutics for treatment of MERS-CoV infections in humans. Like other coronaviruses, the MERS-CoV virion utilizes a large surface spike (S) glycoprotein for interaction with and entry into the target cell. The host cell protein dipeptidyl peptidase 4 (DPP4, aka CD26) was identified as a cellular receptor for MERS-CoV and the specific interaction of the receptor-binding domain (RBD) of MERS-CoV spike protein and DPP4 was determined recently by crystallography.</p><p id="par0010"><bold>Methods & Materials</bold>: Our laboratory has been working to develop rational <italic>in vitro</italic> and <italic>in vivo</italic> models of MERS-CoV infection to allow better understanding of the pathogenesis and transmission potential of virus, and also to evaluate potential therapeutic and vaccine approaches to treat or prevent MERS in humans. Here we present studies focused on characterization of the MERS-CoV receptor, DPP4 in cells from the American mink (<italic>Neovision vision</italic>) as well evaluation of mink as an animal model of MERS-CoV infection. Using multiple approaches we have shown a cell line derived from mink lung epithelium to be susceptible to infection by MERS-CoV.</p><p id="par0015"><bold>Results</bold>: Western blot and PCR analysis of mink lung epithelium cells demonstrate the presence of DPP4, the receptor for MERS-CoV expressed in mink, suggesting a role for this receptor in viral entry in this species. Characterization of the expression of DPP4 in mink cells reveal multiple isoforms, which show varying patterns of expression in cells transfected with each DPP4 isoform using confocal microscopy.</p><p id="par0020"><bold>Conclusion</bold>: In conclusion, evaluation of known DDP4 inhibitors for antiviral activity against MERS-CoV reveal potential therapeutic approaches to treatment of MERS with existing, licensed compounds. Studies underway using mink as an <italic>in vivo</italic> model of MERS-CoV infection and pathogenesis support it's use in discovery and development of therapeutic and vaccines for MERS-CoV in humans.</p></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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