SPRi-Based Strategy to Identify Specific Biomarkers in Systemic Lupus Erythematosus, Rheumatoid Arthritis and Autoimmune Hepatitis
Identifieur interne : 001180 ( Pmc/Checkpoint ); précédent : 001179; suivant : 001181SPRi-Based Strategy to Identify Specific Biomarkers in Systemic Lupus Erythematosus, Rheumatoid Arthritis and Autoimmune Hepatitis
Auteurs : Elvire Beleoken [France] ; Hervé Leh [France] ; Armelle Arnoux [France] ; Béatrice Ducot [France] ; Claude Nogues [France] ; Eleonora De Martin [France] ; Catherine Johanet [France] ; Didier Samuel [France] ; Mohammad Zahid Mustafa [France] ; Jean-Charles Duclos-Vallée [France] ; Malcolm Buckle [France] ; Eric Ballot [France]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is a target for antinuclear autoantibodies in systemic Lupus erythematosus (SLE), rheumatoid arthritis (RA), and autoimmune hepatitis (AIH).
To monitor molecular interactions between peptides spanning the entire sequence of hnRNP A2/B1 and sera from patients and healthy controls.
Sera from 8 patients from each pathology and controls were passed across a surface plasmon resonance Imagery (SPRi) surface containing 39 overlapping peptides of 17 mers covering the human hnRNP B1. Interactions involving the immobilised peptides were followed in real time and dissociation rate constants koff for each interaction were calculated.
Several significant interactions were observed: i) high stability (lower koff values) between P55-70 and the AIH sera compared to controls (p= 0.003); ii) lower stability (higher koff values) between P118-133 and P262-277 and SLE sera, P145-160 and RA sera compared to controls (p=0.006, p=0.002, p=0.007). The binding curves and koff values observed after the formation of complexes with anti-IgM and anti-IgG antibodies and after nuclease treatment of the serum indicate that i) IgM isotypes are prevalent and ii) nucleic acids participate in the interaction between anti-hnRNAP B1 and P55-70 and also between controls and the peptides studied.
These results indicate that P55-70 of hnRNP B1 is a potential biomarker for AIH in immunological tests and suggest the role of circulating nucleic acids, (eg miRNA), present or absent according to the autoimmune disorders and involved in antigen-antibody stability.
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DOI: 10.1371/journal.pone.0084600
PubMed: 24376828
PubMed Central: 3869893
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Mohammad Zahid" sort="Mustafa, Mohammad Zahid" uniqKey="Mustafa M" first="Mohammad Zahid" last="Mustafa">Mohammad Zahid Mustafa</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Research Unit 785, Inserm, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculte de Medecine, University Paris-Sud, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation></author><author><name sortKey="Duclos Vallee, Jean Charles" sort="Duclos Vallee, Jean Charles" uniqKey="Duclos Vallee J" first="Jean-Charles" last="Duclos-Vallée">Jean-Charles Duclos-Vallée</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Research Unit 785, Inserm, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculte de Medecine, University Paris-Sud, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Centre Hépato-Biliaire, AP-HP Hôpital Paul Brousse, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Centre Hépato-Biliaire, AP-HP Hôpital 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Arthritis and Autoimmune Hepatitis</title><author><name sortKey="Beleoken, Elvire" sort="Beleoken, Elvire" uniqKey="Beleoken E" first="Elvire" last="Beleoken">Elvire Beleoken</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Research Unit 785, Inserm, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculte de Medecine, University Paris-Sud, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation></author><author><name sortKey="Leh, Herve" sort="Leh, Herve" uniqKey="Leh H" first="Hervé" last="Leh">Hervé Leh</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Laboratoire de Biologie et de Pharmacologie Appliquée, ENS Cachan - CNRS, Cachan, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie et de Pharmacologie Appliquée, ENS Cachan - CNRS, Cachan</wicri:regionArea><wicri:noRegion>Cachan</wicri:noRegion><wicri:noRegion>Cachan</wicri:noRegion></affiliation></author><author><name sortKey="Arnoux, Armelle" sort="Arnoux, Armelle" uniqKey="Arnoux A" first="Armelle" last="Arnoux">Armelle Arnoux</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Unité de Recherche Clinique, AP-HP Hopital Bicetre, Le Kremlin-Bicetre, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Unité de Recherche Clinique, AP-HP Hopital Bicetre, Le Kremlin-Bicetre</wicri:regionArea><wicri:noRegion>Le Kremlin-Bicetre</wicri:noRegion><wicri:noRegion>Le Kremlin-Bicetre</wicri:noRegion></affiliation></author><author><name sortKey="Ducot, Beatrice" sort="Ducot, Beatrice" uniqKey="Ducot B" first="Béatrice" last="Ducot">Béatrice Ducot</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculte de Medecine, University Paris-Sud, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Unit 1018 - Centre for research in Epidemiology and Population Health, INSERM, Le Kremlin-Bicetre, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Unit 1018 - Centre for research in Epidemiology and Population Health, INSERM, Le Kremlin-Bicetre</wicri:regionArea><wicri:noRegion>Le Kremlin-Bicetre</wicri:noRegion><wicri:noRegion>Le Kremlin-Bicetre</wicri:noRegion></affiliation></author><author><name sortKey="Nogues, Claude" sort="Nogues, Claude" uniqKey="Nogues C" first="Claude" last="Nogues">Claude Nogues</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Laboratoire de Biologie et de Pharmacologie Appliquée, ENS Cachan - CNRS, Cachan, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie et de Pharmacologie Appliquée, ENS Cachan - CNRS, Cachan</wicri:regionArea><wicri:noRegion>Cachan</wicri:noRegion><wicri:noRegion>Cachan</wicri:noRegion></affiliation></author><author><name sortKey="De Martin, Eleonora" sort="De Martin, Eleonora" uniqKey="De Martin E" first="Eleonora" last="De Martin">Eleonora De Martin</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Research Unit 785, Inserm, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculte de Medecine, University Paris-Sud, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff6"><addr-line>Laboratoire Immunologie, AP-HP Hopital Saint-Antoine, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Immunologie, AP-HP Hopital Saint-Antoine, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Johanet, Catherine" sort="Johanet, Catherine" uniqKey="Johanet C" first="Catherine" last="Johanet">Catherine Johanet</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Research Unit 785, Inserm, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff6"><addr-line>Laboratoire Immunologie, AP-HP Hopital Saint-Antoine, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Immunologie, AP-HP Hopital Saint-Antoine, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff7"><addr-line>Faculté de Médecine UFR 967, Université Pierre et Marie Curie, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculté de Médecine UFR 967, Université Pierre et Marie Curie, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName><orgName type="university">Université Pierre-et-Marie-Curie</orgName></affiliation></author><author><name sortKey="Samuel, Didier" sort="Samuel, Didier" uniqKey="Samuel D" first="Didier" last="Samuel">Didier Samuel</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Research Unit 785, Inserm, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculte de Medecine, University Paris-Sud, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Centre Hépato-Biliaire, AP-HP Hôpital Paul Brousse, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Centre Hépato-Biliaire, AP-HP Hôpital Paul Brousse, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation></author><author><name sortKey="Mustafa, Mohammad Zahid" sort="Mustafa, Mohammad Zahid" uniqKey="Mustafa M" first="Mohammad Zahid" last="Mustafa">Mohammad Zahid Mustafa</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Research Unit 785, Inserm, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculte de Medecine, University Paris-Sud, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation></author><author><name sortKey="Duclos Vallee, Jean Charles" sort="Duclos Vallee, Jean Charles" uniqKey="Duclos Vallee J" first="Jean-Charles" last="Duclos-Vallée">Jean-Charles Duclos-Vallée</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Research Unit 785, Inserm, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculte de Medecine, University Paris-Sud, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Centre Hépato-Biliaire, AP-HP Hôpital Paul Brousse, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Centre Hépato-Biliaire, AP-HP Hôpital Paul Brousse, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation></author><author><name sortKey="Buckle, Malcolm" sort="Buckle, Malcolm" uniqKey="Buckle M" first="Malcolm" last="Buckle">Malcolm Buckle</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Laboratoire de Biologie et de Pharmacologie Appliquée, ENS Cachan - CNRS, Cachan, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie et de Pharmacologie Appliquée, ENS Cachan - CNRS, Cachan</wicri:regionArea><wicri:noRegion>Cachan</wicri:noRegion><wicri:noRegion>Cachan</wicri:noRegion></affiliation></author><author><name sortKey="Ballot, Eric" sort="Ballot, Eric" uniqKey="Ballot E" first="Eric" last="Ballot">Eric Ballot</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Research Unit 785, Inserm, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Faculte de Medecine, University Paris-Sud, Villejuif</wicri:regionArea><wicri:noRegion>Villejuif</wicri:noRegion><wicri:noRegion>Villejuif</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff6"><addr-line>Laboratoire Immunologie, AP-HP Hopital Saint-Antoine, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Immunologie, AP-HP Hopital Saint-Antoine, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec sec-type="headed"><title>Background</title><p>Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is a target for antinuclear autoantibodies in systemic Lupus erythematosus (SLE), rheumatoid arthritis (RA), and autoimmune hepatitis (AIH). </p></sec><sec sec-type="headed"><title>Aim</title><p>To monitor molecular interactions between peptides spanning the entire sequence of hnRNP A2/B1 and sera from patients and healthy controls.</p></sec><sec sec-type="headed"><title>Methods</title><p>Sera from 8 patients from each pathology and controls were passed across a surface plasmon resonance Imagery (SPRi) surface containing 39 overlapping peptides of 17 mers covering the human hnRNP B1. Interactions involving the immobilised peptides were followed in real time and dissociation rate constants k<sub>off</sub> for each interaction were calculated.</p></sec><sec sec-type="headed"><title>Results</title><p>Several significant interactions were observed: i) high stability (lower k<sub>off</sub> values) between P<sub>55-70</sub> and the AIH sera compared to controls (p= 0.003); ii) lower stability (higher k<sub>off</sub> values) between P<sub>118-133</sub> and P<sub>262-277</sub> and SLE sera, P<sub>145-160</sub> and RA sera compared to controls (p=0.006, p=0.002, p=0.007). The binding curves and k<sub>off</sub> values observed after the formation of complexes with anti-IgM and anti-IgG antibodies and after nuclease treatment of the serum indicate that i) IgM isotypes are prevalent and ii) nucleic acids participate in the interaction between anti-hnRNAP B1 and P<sub>55-70</sub> and also between controls and the peptides studied.</p></sec><sec sec-type="headed"><title>Conclusions</title><p>These results indicate that P<sub>55-70</sub> of hnRNP B1 is a potential biomarker for AIH in immunological tests and suggest the role of circulating nucleic acids, (eg miRNA), present or absent according to the autoimmune disorders and involved in antigen-antibody stability.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Siapka, S" uniqKey="Siapka S">S Siapka</name></author><author><name sortKey="Patrinou Georgoula, M" uniqKey="Patrinou Georgoula M">M Patrinou-Georgoula</name></author><author><name sortKey="Vlachoyiannopoulos, Pg" uniqKey="Vlachoyiannopoulos P">PG Vlachoyiannopoulos</name></author><author><name sortKey="Guialis, A" uniqKey="Guialis A">A Guialis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steiner, G" uniqKey="Steiner G">G Steiner</name></author><author><name sortKey="Skriner, K" uniqKey="Skriner K">K Skriner</name></author><author><name sortKey="Hassfeld, W" uniqKey="Hassfeld W">W Hassfeld</name></author><author><name sortKey="Smolen, Js" uniqKey="Smolen J">JS Smolen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huguet, S" uniqKey="Huguet S">S Huguet</name></author><author><name sortKey="Labas, V" uniqKey="Labas V">V Labas</name></author><author><name sortKey="Duclos Vallee, Jc" uniqKey="Duclos Vallee J">JC Duclos-Vallee</name></author><author><name sortKey="Bruneel, A" uniqKey="Bruneel A">A Bruneel</name></author><author><name sortKey="Vinh, J" uniqKey="Vinh J">J Vinh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Venables, Jp" uniqKey="Venables J">JP Venables</name></author><author><name sortKey="Koh, Cs" uniqKey="Koh C">CS Koh</name></author><author><name sortKey="Froehlich, U" uniqKey="Froehlich U">U Froehlich</name></author><author><name sortKey="Lapointe, E" uniqKey="Lapointe E">E Lapointe</name></author><author><name sortKey="Couture, S" uniqKey="Couture S">S Couture</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Burd, Cg" uniqKey="Burd C">CG Burd</name></author><author><name sortKey="Swanson, Ms" uniqKey="Swanson M">MS Swanson</name></author><author><name sortKey="Gorlach, M" uniqKey="Gorlach M">M Görlach</name></author><author><name sortKey="Dreyfuss, G" uniqKey="Dreyfuss G">G Dreyfuss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kozu, T" uniqKey="Kozu T">T Kozu</name></author><author><name sortKey="Henrich, B" uniqKey="Henrich B">B Henrich</name></author><author><name sortKey="Sch Fer, Kp" uniqKey="Sch Fer K">KP Schäfer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Landsberg, Mj" uniqKey="Landsberg M">MJ Landsberg</name></author><author><name sortKey="Moran Jones, K" uniqKey="Moran Jones K">K Moran-Jones</name></author><author><name sortKey="Smith, R" uniqKey="Smith R">R Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, Rm" uniqKey="Xu R">RM Xu</name></author><author><name sortKey="Jokhan, L" uniqKey="Jokhan L">L Jokhan</name></author><author><name sortKey="Cheng, X" uniqKey="Cheng X">X Cheng</name></author><author><name sortKey="Mayeda, A" uniqKey="Mayeda A">A Mayeda</name></author><author><name sortKey="Krainer, Ar" uniqKey="Krainer A">AR Krainer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schett, G" uniqKey="Schett G">G Schett</name></author><author><name sortKey="Dumortier" uniqKey="Dumortier">Dumortier</name></author><author><name sortKey="Hoefler, E" uniqKey="Hoefler E">E Hoefler</name></author><author><name sortKey="Muller, S" uniqKey="Muller S">S Muller</name></author><author><name sortKey="Steiner, G" uniqKey="Steiner G">G Steiner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Murray, Js" uniqKey="Murray J">JS Murray</name></author><author><name sortKey="Brown, Jc" uniqKey="Brown J">JC Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Engvall, E" uniqKey="Engvall E">E Engvall</name></author><author><name sortKey="Perlmann, P" uniqKey="Perlmann P">P Perlmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bouffartigues, E" uniqKey="Bouffartigues E">E Bouffartigues</name></author><author><name sortKey="Leh, H" uniqKey="Leh H">H Leh</name></author><author><name sortKey="Anger Leroy, M" uniqKey="Anger Leroy M">M Anger-Leroy</name></author><author><name sortKey="Rimsky, S" uniqKey="Rimsky S">S Rimsky</name></author><author><name sortKey="Buckle, M" uniqKey="Buckle M">M Buckle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nogues, C" uniqKey="Nogues C">C Nogues</name></author><author><name sortKey="Leh, H" uniqKey="Leh H">H Leh</name></author><author><name sortKey="Langendorf, Cg" uniqKey="Langendorf C">CG Langendorf</name></author><author><name sortKey="Law, Rh" uniqKey="Law R">RH Law</name></author><author><name sortKey="Buckle, Am" uniqKey="Buckle A">AM Buckle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nogues, C" uniqKey="Nogues C">C Nogues</name></author><author><name sortKey="Leh, H" uniqKey="Leh H">H Leh</name></author><author><name sortKey="Lautru, J" uniqKey="Lautru J">J Lautru</name></author><author><name sortKey="Delelis, O" uniqKey="Delelis O">O Delelis</name></author><author><name sortKey="Buckle, M" uniqKey="Buckle M">M Buckle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hennes, Em" uniqKey="Hennes E">EM Hennes</name></author><author><name sortKey="Zeniya, M" uniqKey="Zeniya M">M Zeniya</name></author><author><name sortKey="Czaja, Aj" uniqKey="Czaja A">AJ Czaja</name></author><author><name sortKey="Pares, A" uniqKey="Pares A">A Parés</name></author><author><name sortKey="Dalekos, Gn" uniqKey="Dalekos G">GN Dalekos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dreyfuss, G" uniqKey="Dreyfuss G">G Dreyfuss</name></author><author><name sortKey="Kim, Vn" uniqKey="Kim V">VN Kim</name></author><author><name sortKey="Kataoka, N" uniqKey="Kataoka N">N Kataoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moran Jones, K" uniqKey="Moran Jones K">K Moran-Jones</name></author><author><name sortKey="Wayman, L" uniqKey="Wayman L">L Wayman</name></author><author><name sortKey="Kennedy, Dd" uniqKey="Kennedy D">DD Kennedy</name></author><author><name sortKey="Reddel, Rr" uniqKey="Reddel R">RR Reddel</name></author><author><name sortKey="Sara, S" uniqKey="Sara S">S Sara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cooper, Ta" uniqKey="Cooper T">TA Cooper</name></author><author><name sortKey="Wan, L" uniqKey="Wan L">L Wan</name></author><author><name sortKey="Dreyfuss, G" uniqKey="Dreyfuss G">G Dreyfuss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Backes, C" uniqKey="Backes C">C Backes</name></author><author><name sortKey="Ludwig, N" uniqKey="Ludwig N">N Ludwig</name></author><author><name sortKey="Leidinger, P" uniqKey="Leidinger P">P Leidinger</name></author><author><name sortKey="Harz, C" uniqKey="Harz C">C Harz</name></author><author><name sortKey="Hoffmann, J" uniqKey="Hoffmann J">J Hoffmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Skriner, K" uniqKey="Skriner K">K Skriner</name></author><author><name sortKey="Sommergruber, Wh" uniqKey="Sommergruber W">WH Sommergruber</name></author><author><name sortKey="Tremmel, V" uniqKey="Tremmel V">V Tremmel</name></author><author><name sortKey="Fischer, I" uniqKey="Fischer I">I Fischer</name></author><author><name sortKey="Barta, A" uniqKey="Barta A">A Barta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sun, Kh" uniqKey="Sun K">KH Sun</name></author><author><name sortKey="Tang, Sj" uniqKey="Tang S">SJ Tang</name></author><author><name sortKey="Wang, Ys" uniqKey="Wang Y">YS Wang</name></author><author><name sortKey="Lin, Wj" uniqKey="Lin W">WJ Lin</name></author><author><name sortKey="You, Ri" uniqKey="You R">RI You</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Avrameas, S" uniqKey="Avrameas S">S Avrameas</name></author><author><name sortKey="Ternynck, T" uniqKey="Ternynck T">T Ternynck</name></author><author><name sortKey="Tsonis, Ia" uniqKey="Tsonis I">IA Tsonis</name></author><author><name sortKey="Lymberi, P" uniqKey="Lymberi P">P Lymberi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hoffmann, Mh" uniqKey="Hoffmann M">MH Hoffmann</name></author><author><name sortKey="Skriner, K" uniqKey="Skriner K">K Skriner</name></author><author><name sortKey="Herman, S" uniqKey="Herman S">S Herman</name></author><author><name sortKey="Baumann, C" uniqKey="Baumann C">C Baumann</name></author><author><name sortKey="Steiner, Cw" uniqKey="Steiner C">CW Steiner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Avalos Am" uniqKey="Avalos Am">Avalos am</name></author><author><name sortKey="Marshak Rothstein, A" uniqKey="Marshak Rothstein A">A Marshak-Rothstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jakymiw, A" uniqKey="Jakymiw A">A Jakymiw</name></author><author><name sortKey="Ikeda, K" uniqKey="Ikeda K">K Ikeda</name></author><author><name sortKey="Fritzler, Mj" uniqKey="Fritzler M">MJ Fritzler</name></author><author><name sortKey="Reeves, Wh" uniqKey="Reeves W">WH Reeves</name></author><author><name sortKey="Satoh, M" uniqKey="Satoh M">M Satoh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pauley, Km" uniqKey="Pauley K">KM Pauley</name></author><author><name sortKey="Cha, S" uniqKey="Cha S">S Cha</name></author><author><name sortKey="Chan, Ek" uniqKey="Chan E">EK Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ceribelli, A" uniqKey="Ceribelli A">A Ceribelli</name></author><author><name sortKey="Yao, B" uniqKey="Yao B">B Yao</name></author><author><name sortKey="Dominguez Gutierrez, Pr" uniqKey="Dominguez Gutierrez P">PR Dominguez-Gutierrez</name></author><author><name sortKey="Nahid, Ma" uniqKey="Nahid M">MA Nahid</name></author><author><name sortKey="Satoh, M" uniqKey="Satoh M">M Satoh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belver, L" uniqKey="Belver L">L Belver</name></author><author><name sortKey="De Yebenes, Vg" uniqKey="De Yebenes V">VG de Yébenes</name></author><author><name sortKey="Ramiro, Ar" uniqKey="Ramiro A">AR Ramiro</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24376828</article-id><article-id pub-id-type="pmc">3869893</article-id><article-id pub-id-type="publisher-id">PONE-D-13-28035</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0084600</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>SPRi-Based Strategy to Identify Specific Biomarkers in Systemic Lupus Erythematosus, Rheumatoid Arthritis and Autoimmune Hepatitis</article-title><alt-title alt-title-type="running-head">hnRNP B1 Reactivity in Autoimmune Diseases by SPRi</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Beleoken</surname><given-names>Elvire</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Leh</surname><given-names>Hervé</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Arnoux</surname><given-names>Armelle</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Ducot</surname><given-names>Béatrice</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Nogues</surname><given-names>Claude</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>De Martin</surname><given-names>Eleonora</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Johanet</surname><given-names>Catherine</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff6"><sup>6</sup></xref><xref ref-type="aff" rid="aff7"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>Samuel</surname><given-names>Didier</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff8"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Mustafa</surname><given-names>Mohammad Zahid</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Duclos-Vallée</surname><given-names>Jean-Charles</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff8"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Buckle</surname><given-names>Malcolm</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ballot</surname><given-names>Eric</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff6"><sup>6</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Research Unit 785, Inserm, Villejuif, France</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Faculte de Medecine, University Paris-Sud, Villejuif, France</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Laboratoire de Biologie et de Pharmacologie Appliquée, ENS Cachan - CNRS, Cachan, France</addr-line></aff><aff id="aff4"><label>4</label><addr-line>Unit 1018 - Centre for research in Epidemiology and Population Health, INSERM, Le Kremlin-Bicetre, France</addr-line></aff><aff id="aff5"><label>5</label><addr-line>Unité de Recherche Clinique, AP-HP Hopital Bicetre, Le Kremlin-Bicetre, France</addr-line></aff><aff id="aff6"><label>6</label><addr-line>Laboratoire Immunologie, AP-HP Hopital Saint-Antoine, Paris, France</addr-line></aff><aff id="aff7"><label>7</label><addr-line>Faculté de Médecine UFR 967, Université Pierre et Marie Curie, Paris, France</addr-line></aff><aff id="aff8"><label>8</label><addr-line>Centre Hépato-Biliaire, AP-HP Hôpital Paul Brousse, Villejuif, France</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Bobé</surname><given-names>Pierre</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>INSERM-Université Paris-Sud, France</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>eric.ballot@sat.aphp.fr</email></corresp><fn fn-type="COI-statement"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: E. Beleoken HL CN EDM JCDV MB E. Ballot. Performed the experiments: E. Beleoken HL CN EDM. Analyzed the data: E. Beleoken HL EDM AA BD CJ DS MZM JCDV MB E. Ballot. Contributed reagents/materials/analysis tools: HL AA BD CN CJ DS MB. Wrote the manuscript: E. Beleoken HL JCDV MB E. Ballot. Read and critically revised the manuscript : AA, BD, CN, EdM, CJ, DS, MZM, JCDV, MB, EBa. </p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>20</day><month>12</month><year>2013</year></pub-date><volume>8</volume><issue>12</issue><elocation-id>e84600</elocation-id><history><date date-type="received"><day>8</day><month>7</month><year>2013</year></date><date date-type="accepted"><day>16</day><month>11</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 Beleoken et al</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>Beleoken et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.</license-p></license></permissions><abstract><sec sec-type="headed"><title>Background</title><p>Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is a target for antinuclear autoantibodies in systemic Lupus erythematosus (SLE), rheumatoid arthritis (RA), and autoimmune hepatitis (AIH). </p></sec><sec sec-type="headed"><title>Aim</title><p>To monitor molecular interactions between peptides spanning the entire sequence of hnRNP A2/B1 and sera from patients and healthy controls.</p></sec><sec sec-type="headed"><title>Methods</title><p>Sera from 8 patients from each pathology and controls were passed across a surface plasmon resonance Imagery (SPRi) surface containing 39 overlapping peptides of 17 mers covering the human hnRNP B1. Interactions involving the immobilised peptides were followed in real time and dissociation rate constants k<sub>off</sub> for each interaction were calculated.</p></sec><sec sec-type="headed"><title>Results</title><p>Several significant interactions were observed: i) high stability (lower k<sub>off</sub> values) between P<sub>55-70</sub> and the AIH sera compared to controls (p= 0.003); ii) lower stability (higher k<sub>off</sub> values) between P<sub>118-133</sub> and P<sub>262-277</sub> and SLE sera, P<sub>145-160</sub> and RA sera compared to controls (p=0.006, p=0.002, p=0.007). The binding curves and k<sub>off</sub> values observed after the formation of complexes with anti-IgM and anti-IgG antibodies and after nuclease treatment of the serum indicate that i) IgM isotypes are prevalent and ii) nucleic acids participate in the interaction between anti-hnRNAP B1 and P<sub>55-70</sub> and also between controls and the peptides studied.</p></sec><sec sec-type="headed"><title>Conclusions</title><p>These results indicate that P<sub>55-70</sub> of hnRNP B1 is a potential biomarker for AIH in immunological tests and suggest the role of circulating nucleic acids, (eg miRNA), present or absent according to the autoimmune disorders and involved in antigen-antibody stability.</p></sec></abstract><funding-group><funding-statement>The authors have no support or funding to report.</funding-statement></funding-group></article-meta></front></pmc><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement><orgName><li>Université Pierre-et-Marie-Curie</li></orgName></list><tree><country name="France"><noRegion><name sortKey="Beleoken, Elvire" sort="Beleoken, Elvire" uniqKey="Beleoken E" first="Elvire" last="Beleoken">Elvire Beleoken</name></noRegion><name sortKey="Arnoux, Armelle" sort="Arnoux, Armelle" uniqKey="Arnoux A" first="Armelle" last="Arnoux">Armelle Arnoux</name><name sortKey="Ballot, Eric" sort="Ballot, Eric" uniqKey="Ballot E" first="Eric" last="Ballot">Eric Ballot</name><name sortKey="Ballot, Eric" sort="Ballot, Eric" uniqKey="Ballot E" first="Eric" last="Ballot">Eric Ballot</name><name sortKey="Ballot, Eric" sort="Ballot, Eric" uniqKey="Ballot E" first="Eric" last="Ballot">Eric Ballot</name><name sortKey="Beleoken, Elvire" sort="Beleoken, Elvire" uniqKey="Beleoken E" first="Elvire" last="Beleoken">Elvire Beleoken</name><name sortKey="Buckle, Malcolm" sort="Buckle, Malcolm" uniqKey="Buckle M" first="Malcolm" last="Buckle">Malcolm Buckle</name><name sortKey="De Martin, Eleonora" sort="De Martin, Eleonora" uniqKey="De Martin E" first="Eleonora" last="De Martin">Eleonora De Martin</name><name sortKey="De Martin, Eleonora" sort="De Martin, Eleonora" uniqKey="De Martin E" first="Eleonora" last="De Martin">Eleonora De Martin</name><name sortKey="De Martin, Eleonora" sort="De Martin, Eleonora" uniqKey="De Martin E" first="Eleonora" last="De Martin">Eleonora De Martin</name><name sortKey="Duclos Vallee, Jean Charles" sort="Duclos Vallee, Jean Charles" uniqKey="Duclos Vallee J" first="Jean-Charles" last="Duclos-Vallée">Jean-Charles Duclos-Vallée</name><name sortKey="Duclos Vallee, Jean Charles" sort="Duclos Vallee, Jean Charles" uniqKey="Duclos Vallee J" first="Jean-Charles" last="Duclos-Vallée">Jean-Charles Duclos-Vallée</name><name sortKey="Duclos Vallee, Jean Charles" sort="Duclos Vallee, Jean Charles" uniqKey="Duclos Vallee J" first="Jean-Charles" last="Duclos-Vallée">Jean-Charles Duclos-Vallée</name><name sortKey="Ducot, Beatrice" sort="Ducot, Beatrice" uniqKey="Ducot B" first="Béatrice" last="Ducot">Béatrice Ducot</name><name sortKey="Ducot, Beatrice" sort="Ducot, Beatrice" uniqKey="Ducot B" first="Béatrice" last="Ducot">Béatrice Ducot</name><name sortKey="Johanet, Catherine" sort="Johanet, Catherine" uniqKey="Johanet C" first="Catherine" last="Johanet">Catherine Johanet</name><name sortKey="Johanet, Catherine" sort="Johanet, Catherine" uniqKey="Johanet C" first="Catherine" last="Johanet">Catherine Johanet</name><name sortKey="Johanet, Catherine" sort="Johanet, Catherine" uniqKey="Johanet C" first="Catherine" last="Johanet">Catherine Johanet</name><name sortKey="Leh, Herve" sort="Leh, Herve" uniqKey="Leh H" first="Hervé" last="Leh">Hervé Leh</name><name sortKey="Mustafa, Mohammad Zahid" sort="Mustafa, Mohammad Zahid" uniqKey="Mustafa M" first="Mohammad Zahid" last="Mustafa">Mohammad Zahid Mustafa</name><name sortKey="Mustafa, Mohammad Zahid" sort="Mustafa, Mohammad Zahid" uniqKey="Mustafa M" first="Mohammad Zahid" last="Mustafa">Mohammad Zahid Mustafa</name><name sortKey="Nogues, Claude" sort="Nogues, Claude" uniqKey="Nogues C" first="Claude" last="Nogues">Claude Nogues</name><name sortKey="Samuel, Didier" sort="Samuel, Didier" uniqKey="Samuel D" first="Didier" last="Samuel">Didier Samuel</name><name sortKey="Samuel, Didier" sort="Samuel, Didier" uniqKey="Samuel D" first="Didier" last="Samuel">Didier Samuel</name><name sortKey="Samuel, Didier" sort="Samuel, Didier" uniqKey="Samuel D" first="Didier" last="Samuel">Didier Samuel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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