Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak
Identifieur interne : 000B23 ( Pmc/Checkpoint ); précédent : 000B22; suivant : 000B24Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak
Auteurs : Wan Beom Park [Corée du Sud] ; Nak-Jung Kwon [Corée du Sud] ; Pyoeng Gyun Choe [Corée du Sud] ; Su-Jin Choi [Corée du Sud] ; Hong Sang Oh [Corée du Sud] ; Sang Min Lee [Corée du Sud] ; Hyonyong Chong [Corée du Sud] ; Jong-Il Kim [Corée du Sud] ; Kyoung-Ho Song [Corée du Sud] ; Ji Hwan Bang [Corée du Sud] ; Eu Suk Kim [Corée du Sud] ; Hong-Bin Kim [Corée du Sud] ; Sang Won Park [Corée du Sud] ; Nam Joong Kim [Corée du Sud] ; Myoung-Don Oh [Corée du Sud]Source :
- Journal of Korean Medical Science [ 1011-8934 ] ; 2016.
Abstract
During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.
Url:
DOI: 10.3346/jkms.2016.31.2.315
PubMed: 26839489
PubMed Central: 4729515
Affiliations:
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<author><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung-Don" last="Oh">Myoung-Don Oh</name>
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<front><div type="abstract" xml:lang="en"><p>During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.</p>
</div>
</front>
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<pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Korean Med Sci</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Korean Med. Sci</journal-id>
<journal-id journal-id-type="publisher-id">JKMS</journal-id>
<journal-title-group><journal-title>Journal of Korean Medical Science</journal-title>
</journal-title-group>
<issn pub-type="ppub">1011-8934</issn>
<issn pub-type="epub">1598-6357</issn>
<publisher><publisher-name>The Korean Academy of Medical Sciences</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26839489</article-id>
<article-id pub-id-type="pmc">4729515</article-id>
<article-id pub-id-type="doi">10.3346/jkms.2016.31.2.315</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Brief Communication</subject>
<subj-group subj-group-type="subheading"><subject>Infectious Diseases, Microbiology & Parasitology</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0003-0022-9625</contrib-id>
<name><surname>Park</surname>
<given-names>Wan Beom</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A2">2</xref>
<xref ref-type="author-notes" rid="afn1"><sup>*</sup>
</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0003-0874-4146</contrib-id>
<name><surname>Kwon</surname>
<given-names>Nak-Jung</given-names>
</name>
<xref ref-type="aff" rid="A3">3</xref>
<xref ref-type="author-notes" rid="afn1"><sup>*</sup>
</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-6794-7918</contrib-id>
<name><surname>Choe</surname>
<given-names>Pyoeng Gyun</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-8732-3950</contrib-id>
<name><surname>Choi</surname>
<given-names>Su-Jin</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-4535-6305</contrib-id>
<name><surname>Oh</surname>
<given-names>Hong Sang</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-1388-9318</contrib-id>
<name><surname>Lee</surname>
<given-names>Sang Min</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-6928-8805</contrib-id>
<name><surname>Chong</surname>
<given-names>Hyonyong</given-names>
</name>
<xref ref-type="aff" rid="A3">3</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-7240-3744</contrib-id>
<name><surname>Kim</surname>
<given-names>Jong-Il</given-names>
</name>
<xref ref-type="aff" rid="A4">4</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-4517-3840</contrib-id>
<name><surname>Song</surname>
<given-names>Kyoung-Ho</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-7628-1182</contrib-id>
<name><surname>Bang</surname>
<given-names>Ji Hwan</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-7132-0157</contrib-id>
<name><surname>Kim</surname>
<given-names>Eu Suk</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-6262-372X</contrib-id>
<name><surname>Kim</surname>
<given-names>Hong-Bin</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-0550-1897</contrib-id>
<name><surname>Park</surname>
<given-names>Sang Won</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-6793-9467</contrib-id>
<name><surname>Kim</surname>
<given-names>Nam Joong</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-2344-7695</contrib-id>
<name><surname>Oh</surname>
<given-names>Myoung-don</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</aff>
<aff id="A2"><label>2</label>
Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</aff>
<aff id="A3"><label>3</label>
Macrogen, Seoul, Korea.</aff>
<aff id="A4"><label>4</label>
Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.</aff>
<author-notes><corresp>Address for Correspondence: Myoung-don Oh, MD. Department of Internal Medicine, Seoul National University College of Medicine, 103, Daehak-ro, Jongno-gu, Seoul 03080, Korea. Tel: +82.2-2072-2945, Fax: +82.2-762-9662, <email>mdohmd@snu.ac.kr</email>
</corresp>
<fn id="afn1" fn-type="equal"><p>*Wan Beom Park and Nak-Jung Kwon equally contributed to the study.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>2</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub"><day>14</day>
<month>1</month>
<year>2016</year>
</pub-date>
<volume>31</volume>
<issue>2</issue>
<fpage>315</fpage>
<lpage>320</lpage>
<history><date date-type="received"><day>29</day>
<month>12</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>11</day>
<month>1</month>
<year>2016</year>
</date>
</history>
<permissions><copyright-statement>© 2016 The Korean Academy of Medical Sciences.</copyright-statement>
<copyright-year>2016</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/4.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/4.0/">http://creativecommons.org/licenses/by-nc/4.0/</ext-link>
) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<abstract><p>During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.</p>
</abstract>
<kwd-group kwd-group-type="author"><kwd>Middle East Respiratory Syndrome</kwd>
<kwd>Middle East Respiratory Syndrome Coronavirus</kwd>
<kwd>Microscopy, Electron</kwd>
<kwd>Phylogeny</kwd>
<kwd>Korea</kwd>
</kwd-group>
</article-meta>
</front>
<floats-group><fig id="F1" fig-type="figure" orientation="portrait" position="float"><label>Fig. 1</label>
<caption><title>Cytopathic effects of MERS-CoV in Vero cell cultures and Electron microscopy image of MERS-CoV. Vero cells were inoculated with oropharyngeal swab sample. (<bold>A</bold>
) Vero cell cultures in negative control. (<bold>B</bold>
) Cytopathic effects (rounding and detachment of cells) in Vero cell cultures 3 days after inoculation of the sample. (<bold>C, D</bold>
) Transmission electron microscopy image of Vero cells infected with MERS-CoV. White arrow denotes nuclear membrane. Brown scale bar indicates 100 μm (A and B). Black scale bar indicates 500 nm (<bold>C</bold>
) and white scale bar does 200 nm (<bold>D</bold>
).</title>
</caption>
<graphic xlink:href="jkms-31-315-g001"></graphic>
</fig>
<fig id="F2" fig-type="figure" orientation="portrait" position="float"><label>Fig. 2</label>
<caption><title>Molecular phylogenetic analysis. Phylogenetic tree on complete genome (<bold>A</bold>
), S genes (<bold>B</bold>
), and ORF1ab genes (<bold>C</bold>
) for the 101 gene sequences of MERS-CoV. The evolutionary history was inferred by using the maximum likelihood method based on the Tamura-Nei model (<xref rid="B16" ref-type="bibr">16</xref>
). Evolutionary analyses were conducted in MEGA6 (<xref rid="B17" ref-type="bibr">17</xref>
). Red box indicates our virus isolate.</title>
</caption>
<graphic xlink:href="jkms-31-315-g002"></graphic>
</fig>
<table-wrap id="T1" orientation="portrait" position="float"><label>Table 1</label>
<caption><title>PCR primer pairs used in this study</title>
</caption>
<alternatives><graphic xlink:href="jkms-31-315-i001"></graphic>
<table frame="hsides" rules="rows"><thead><tr><th valign="top" align="left" rowspan="1" colspan="1">No.</th>
<th valign="top" align="center" rowspan="1" colspan="1">Forward</th>
<th valign="top" align="center" rowspan="1" colspan="1">sequence (5'-->3')</th>
<th valign="top" align="center" rowspan="1" colspan="1">Reverse</th>
<th valign="top" align="center" rowspan="1" colspan="1">sequence (5'-->3')</th>
</tr>
</thead>
<tbody><tr><td valign="top" align="right" rowspan="1" colspan="1">1</td>
<td valign="top" align="left" rowspan="1" colspan="1">FEP_1_F</td>
<td valign="top" align="left" rowspan="1" colspan="1">GATTTAAGTGAATAGCTTGGCTATCTC</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_70342_1_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">GGAATATTAGAGACTCCCTGCCG</td>
</tr>
<tr><td valign="top" align="right" rowspan="1" colspan="1">2</td>
<td valign="top" align="left" rowspan="1" colspan="1">2497F</td>
<td valign="top" align="left" rowspan="1" colspan="1">TCCCATCGGGAACCTATTACTGTG</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68466_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">TGTAACCACCATTAGTGCGGAC</td>
</tr>
<tr><td valign="top" align="right" rowspan="1" colspan="1">3</td>
<td valign="top" align="left" rowspan="1" colspan="1">4477F</td>
<td valign="top" align="left" rowspan="1" colspan="1">ACGTTAAGTTAAACCCTTCAGAAG</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_67825_1_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">AATGAAGCCCTAAATAGTAACTTCACT</td>
</tr>
<tr><td valign="top" align="right" rowspan="2" colspan="1">4</td>
<td valign="top" align="left" rowspan="1" colspan="1">new2_15F_6427</td>
<td valign="top" align="left" rowspan="1" colspan="1">TGCTTAGATTGCACACCGTT</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68526_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">AGGTGGTTAACCGGAAAGCTAAA</td>
</tr>
<tr><td valign="top" align="left" rowspan="1" colspan="1">6506F</td>
<td valign="top" align="left" rowspan="1" colspan="1">AGAATTTGCTACCCGCACTTTCACTG</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68526_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">AGGTGGTTAACCGGAAAGCTAAA</td>
</tr>
<tr><td valign="top" align="right" rowspan="2" colspan="1">5</td>
<td valign="top" align="left" rowspan="1" colspan="1">new2_19F_8393</td>
<td valign="top" align="left" rowspan="1" colspan="1">GGATGCACTTAAACGACAGA</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_33984_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">GTGTAACCAACACTACCACAAGAAC</td>
</tr>
<tr><td valign="top" align="left" rowspan="1" colspan="1">8496F</td>
<td valign="top" align="left" rowspan="1" colspan="1">TGGTGCTCCTACATGGTTTAATGCG</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_33984_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">GTGTAACCAACACTACCACAAGAAC</td>
</tr>
<tr><td valign="top" align="right" rowspan="1" colspan="1">6</td>
<td valign="top" align="left" rowspan="1" colspan="1">10477F</td>
<td valign="top" align="left" rowspan="1" colspan="1">ACACCAAGGAGGGTAGTGTGATC</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_67887_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">GAATTACAACGCGAAGTTTATTTGAAG</td>
</tr>
<tr><td valign="top" align="right" rowspan="2" colspan="1">7</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_2977_F</td>
<td valign="top" align="left" rowspan="1" colspan="1">AAGGCTTTGCAGAAGGCTGTTA</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68115_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">GAATTACAACGCGAAGTTTATTTGAAG</td>
</tr>
<tr><td valign="top" align="left" rowspan="1" colspan="1">12488F</td>
<td valign="top" align="left" rowspan="1" colspan="1">AGGTAGTCACATATCCCTCGCTTAAC</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68115_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">TCATCAACTCCTTAAGAGAGAGCCTAT</td>
</tr>
<tr><td valign="top" align="right" rowspan="1" colspan="1">8</td>
<td valign="top" align="left" rowspan="1" colspan="1">14481F</td>
<td valign="top" align="left" rowspan="1" colspan="1">TGGATGTTAGTCTCCATAGACATAG</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_34102_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">TGTAATCACCACTTTCAGTCCAGT</td>
</tr>
<tr><td valign="top" align="right" rowspan="1" colspan="1">9</td>
<td valign="top" align="left" rowspan="1" colspan="1">16476F</td>
<td valign="top" align="left" rowspan="1" colspan="1">TCGGCTTATACAAGAATATGTGCAC</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_981_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">CATGAGCCCAACAAACAAACGTA</td>
</tr>
<tr><td valign="top" align="right" rowspan="1" colspan="1">10</td>
<td valign="top" align="left" rowspan="1" colspan="1">18490F</td>
<td valign="top" align="left" rowspan="1" colspan="1">ACGACGTATAGTGCAAATGTTGTC</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_69145_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">AGCTTTAAATCTATAACAGAACACACC</td>
</tr>
<tr><td valign="top" align="right" rowspan="2" colspan="1">11</td>
<td valign="top" align="left" rowspan="1" colspan="1">new2_42F_20357</td>
<td valign="top" align="left" rowspan="1" colspan="1">AAGAAGCAACAGGAAGGTCA</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_34878_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">TAGAAGGCAGCCCAAGCTTTT</td>
</tr>
<tr><td valign="top" align="left" rowspan="1" colspan="1">20490F</td>
<td valign="top" align="left" rowspan="1" colspan="1">AAGACCTTGGCGTAGTATCCAAGG</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_34878_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">TAGAAGGCAGCCCAAGCTTTT</td>
</tr>
<tr><td valign="top" align="right" rowspan="2" colspan="1">12</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_67088_1_F</td>
<td valign="top" align="left" rowspan="1" colspan="1">CCACCTTGCCTGTTTATGATACTATTA</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_72579_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">CTGTTTGCATAGCTCCCAGAG</td>
</tr>
<tr><td valign="top" align="left" rowspan="1" colspan="1">22481F</td>
<td valign="top" align="left" rowspan="1" colspan="1">TGATTTGTCACAACTCCACTGC</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_72579_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">CTGTTTGCATAGCTCCCAGAG</td>
</tr>
<tr><td valign="top" align="right" rowspan="2" colspan="1">13</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_66781_F</td>
<td valign="top" align="left" rowspan="1" colspan="1">TGGACTGCTGGCTTATCCTC</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_66820_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">GCTTAAATCTATGTATGTTAGCACAGT</td>
</tr>
<tr><td valign="top" align="left" rowspan="1" colspan="1">24512F</td>
<td valign="top" align="left" rowspan="1" colspan="1">TCAGAAGGTTCAGGATGCTGTGAAC</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_66820_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">GCTTAAATCTATGTATGTTAGCACAGT</td>
</tr>
<tr><td valign="top" align="right" rowspan="1" colspan="1">14</td>
<td valign="top" align="left" rowspan="1" colspan="1">26470F</td>
<td valign="top" align="left" rowspan="1" colspan="1">TGAGTTCGCCTTGCTGCGCAAAAC</td>
<td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_69858_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">TGTAATTACCTGCCTTATATCTATGGT</td>
</tr>
<tr><td valign="top" align="right" rowspan="2" colspan="1">15</td>
<td valign="top" align="left" rowspan="1" colspan="1">new2_59F_28427</td>
<td valign="top" align="left" rowspan="1" colspan="1">GGCAAAGCTACGGAACTAAT</td>
<td valign="top" align="left" rowspan="1" colspan="1">FEP_3_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">GCAAATCATCTAATTAGCCTAATCTAATTG</td>
</tr>
<tr><td valign="top" align="left" rowspan="1" colspan="1">28490F</td>
<td valign="top" align="left" rowspan="1" colspan="1">AACTTGCATTGCTTCGAGCTTAGG</td>
<td valign="top" align="left" rowspan="1" colspan="1">FEP_3_R</td>
<td valign="top" align="left" rowspan="1" colspan="1">GCAAATCATCTAATTAGCCTAATCTAATTG</td>
</tr>
</tbody>
</table>
</alternatives>
</table-wrap>
</floats-group>
</pmc>
<affiliations><list><country><li>Corée du Sud</li>
</country>
<region><li>Région capitale de Séoul</li>
</region>
<settlement><li>Séoul</li>
</settlement>
</list>
<tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Park, Wan Beom" sort="Park, Wan Beom" uniqKey="Park W" first="Wan Beom" last="Park">Wan Beom Park</name>
</region>
<name sortKey="Bang, Ji Hwan" sort="Bang, Ji Hwan" uniqKey="Bang J" first="Ji Hwan" last="Bang">Ji Hwan Bang</name>
<name sortKey="Choe, Pyoeng Gyun" sort="Choe, Pyoeng Gyun" uniqKey="Choe P" first="Pyoeng Gyun" last="Choe">Pyoeng Gyun Choe</name>
<name sortKey="Choi, Su Jin" sort="Choi, Su Jin" uniqKey="Choi S" first="Su-Jin" last="Choi">Su-Jin Choi</name>
<name sortKey="Chong, Hyonyong" sort="Chong, Hyonyong" uniqKey="Chong H" first="Hyonyong" last="Chong">Hyonyong Chong</name>
<name sortKey="Kim, Eu Suk" sort="Kim, Eu Suk" uniqKey="Kim E" first="Eu Suk" last="Kim">Eu Suk Kim</name>
<name sortKey="Kim, Hong Bin" sort="Kim, Hong Bin" uniqKey="Kim H" first="Hong-Bin" last="Kim">Hong-Bin Kim</name>
<name sortKey="Kim, Jong Il" sort="Kim, Jong Il" uniqKey="Kim J" first="Jong-Il" last="Kim">Jong-Il Kim</name>
<name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name>
<name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name>
<name sortKey="Kwon, Nak Jung" sort="Kwon, Nak Jung" uniqKey="Kwon N" first="Nak-Jung" last="Kwon">Nak-Jung Kwon</name>
<name sortKey="Lee, Sang Min" sort="Lee, Sang Min" uniqKey="Lee S" first="Sang Min" last="Lee">Sang Min Lee</name>
<name sortKey="Oh, Hong Sang" sort="Oh, Hong Sang" uniqKey="Oh H" first="Hong Sang" last="Oh">Hong Sang Oh</name>
<name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung-Don" last="Oh">Myoung-Don Oh</name>
<name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung-Don" last="Oh">Myoung-Don Oh</name>
<name sortKey="Park, Sang Won" sort="Park, Sang Won" uniqKey="Park S" first="Sang Won" last="Park">Sang Won Park</name>
<name sortKey="Park, Wan Beom" sort="Park, Wan Beom" uniqKey="Park W" first="Wan Beom" last="Park">Wan Beom Park</name>
<name sortKey="Song, Kyoung Ho" sort="Song, Kyoung Ho" uniqKey="Song K" first="Kyoung-Ho" last="Song">Kyoung-Ho Song</name>
</country>
</tree>
</affiliations>
</record>
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