Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak
Identifieur interne : 000B23 ( Pmc/Checkpoint ); précédent : 000B22; suivant : 000B24Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak
Auteurs : Wan Beom Park [Corée du Sud] ; Nak-Jung Kwon [Corée du Sud] ; Pyoeng Gyun Choe [Corée du Sud] ; Su-Jin Choi [Corée du Sud] ; Hong Sang Oh [Corée du Sud] ; Sang Min Lee [Corée du Sud] ; Hyonyong Chong [Corée du Sud] ; Jong-Il Kim [Corée du Sud] ; Kyoung-Ho Song [Corée du Sud] ; Ji Hwan Bang [Corée du Sud] ; Eu Suk Kim [Corée du Sud] ; Hong-Bin Kim [Corée du Sud] ; Sang Won Park [Corée du Sud] ; Nam Joong Kim [Corée du Sud] ; Myoung-Don Oh [Corée du Sud]Source :
- Journal of Korean Medical Science [ 1011-8934 ] ; 2016.
Abstract
During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.
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DOI: 10.3346/jkms.2016.31.2.315
PubMed: 26839489
PubMed Central: 4729515
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Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Chong, Hyonyong" sort="Chong, Hyonyong" uniqKey="Chong H" first="Hyonyong" last="Chong">Hyonyong Chong</name><affiliation wicri:level="3"><nlm:aff id="A3">Macrogen, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Macrogen, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Jong Il" sort="Kim, Jong Il" uniqKey="Kim J" first="Jong-Il" last="Kim">Jong-Il Kim</name><affiliation wicri:level="3"><nlm:aff id="A4">Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Song, Kyoung Ho" sort="Song, Kyoung Ho" uniqKey="Song K" first="Kyoung-Ho" last="Song">Kyoung-Ho Song</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Bang, Ji Hwan" sort="Bang, Ji Hwan" uniqKey="Bang J" first="Ji Hwan" last="Bang">Ji Hwan Bang</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Eu Suk" sort="Kim, Eu Suk" uniqKey="Kim E" first="Eu Suk" last="Kim">Eu Suk Kim</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Hong Bin" sort="Kim, Hong Bin" uniqKey="Kim H" first="Hong-Bin" last="Kim">Hong-Bin Kim</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Park, Sang Won" sort="Park, Sang Won" uniqKey="Park S" first="Sang Won" last="Park">Sang Won Park</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement 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Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="A2">Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kwon, Nak Jung" sort="Kwon, Nak Jung" uniqKey="Kwon N" first="Nak-Jung" last="Kwon">Nak-Jung Kwon</name><affiliation wicri:level="3"><nlm:aff id="A3">Macrogen, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Macrogen, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Choe, Pyoeng Gyun" sort="Choe, Pyoeng Gyun" uniqKey="Choe P" first="Pyoeng Gyun" last="Choe">Pyoeng Gyun Choe</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Choi, Su Jin" sort="Choi, Su Jin" uniqKey="Choi S" first="Su-Jin" last="Choi">Su-Jin Choi</name><affiliation wicri:level="3"><nlm:aff id="A2">Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Oh, Hong Sang" sort="Oh, Hong Sang" uniqKey="Oh H" first="Hong Sang" last="Oh">Hong Sang Oh</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Lee, Sang Min" sort="Lee, Sang Min" uniqKey="Lee S" first="Sang Min" last="Lee">Sang Min Lee</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Chong, Hyonyong" sort="Chong, Hyonyong" uniqKey="Chong H" first="Hyonyong" last="Chong">Hyonyong Chong</name><affiliation wicri:level="3"><nlm:aff id="A3">Macrogen, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Macrogen, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Jong Il" sort="Kim, Jong Il" uniqKey="Kim J" first="Jong-Il" last="Kim">Jong-Il Kim</name><affiliation wicri:level="3"><nlm:aff id="A4">Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Song, Kyoung Ho" sort="Song, Kyoung Ho" uniqKey="Song K" first="Kyoung-Ho" last="Song">Kyoung-Ho Song</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Bang, Ji Hwan" sort="Bang, Ji Hwan" uniqKey="Bang J" first="Ji Hwan" last="Bang">Ji Hwan Bang</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Eu Suk" sort="Kim, Eu Suk" uniqKey="Kim E" first="Eu Suk" last="Kim">Eu Suk Kim</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Hong Bin" sort="Kim, Hong Bin" uniqKey="Kim H" first="Hong-Bin" last="Kim">Hong-Bin Kim</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Park, Sang Won" sort="Park, Sang Won" uniqKey="Park S" first="Sang Won" last="Park">Sang Won Park</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="A2">Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung-Don" last="Oh">Myoung-Don Oh</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="A2">Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Journal of Korean Medical Science</title><idno type="ISSN">1011-8934</idno><idno type="eISSN">1598-6357</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="To, Kk" uniqKey="To K">KK To</name></author><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zumla, A" uniqKey="Zumla A">A Zumla</name></author><author><name sortKey="Hui, Ds" uniqKey="Hui D">DS Hui</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Korean Med Sci</journal-id><journal-id journal-id-type="iso-abbrev">J. Korean Med. Sci</journal-id><journal-id journal-id-type="publisher-id">JKMS</journal-id><journal-title-group><journal-title>Journal of Korean Medical Science</journal-title></journal-title-group><issn pub-type="ppub">1011-8934</issn><issn pub-type="epub">1598-6357</issn><publisher><publisher-name>The Korean Academy of Medical Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26839489</article-id><article-id pub-id-type="pmc">4729515</article-id><article-id pub-id-type="doi">10.3346/jkms.2016.31.2.315</article-id><article-categories><subj-group subj-group-type="heading"><subject>Brief Communication</subject><subj-group subj-group-type="subheading"><subject>Infectious Diseases, Microbiology & Parasitology</subject></subj-group></subj-group></article-categories><title-group><article-title>Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak</article-title></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0003-0022-9625</contrib-id><name><surname>Park</surname><given-names>Wan Beom</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A2">2</xref><xref ref-type="author-notes" rid="afn1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0003-0874-4146</contrib-id><name><surname>Kwon</surname><given-names>Nak-Jung</given-names></name><xref ref-type="aff" rid="A3">3</xref><xref ref-type="author-notes" rid="afn1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-6794-7918</contrib-id><name><surname>Choe</surname><given-names>Pyoeng Gyun</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-8732-3950</contrib-id><name><surname>Choi</surname><given-names>Su-Jin</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-4535-6305</contrib-id><name><surname>Oh</surname><given-names>Hong Sang</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-1388-9318</contrib-id><name><surname>Lee</surname><given-names>Sang Min</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-6928-8805</contrib-id><name><surname>Chong</surname><given-names>Hyonyong</given-names></name><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-7240-3744</contrib-id><name><surname>Kim</surname><given-names>Jong-Il</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-4517-3840</contrib-id><name><surname>Song</surname><given-names>Kyoung-Ho</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-7628-1182</contrib-id><name><surname>Bang</surname><given-names>Ji Hwan</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-7132-0157</contrib-id><name><surname>Kim</surname><given-names>Eu Suk</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-6262-372X</contrib-id><name><surname>Kim</surname><given-names>Hong-Bin</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-0550-1897</contrib-id><name><surname>Park</surname><given-names>Sang Won</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0001-6793-9467</contrib-id><name><surname>Kim</surname><given-names>Nam Joong</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid" authenticated="true">http://orcid.org/0000-0002-2344-7695</contrib-id><name><surname>Oh</surname><given-names>Myoung-don</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A2">2</xref></contrib></contrib-group><aff id="A1"><label>1</label>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</aff><aff id="A2"><label>2</label>Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</aff><aff id="A3"><label>3</label>Macrogen, Seoul, Korea.</aff><aff id="A4"><label>4</label>Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.</aff><author-notes><corresp>Address for Correspondence: Myoung-don Oh, MD. Department of Internal Medicine, Seoul National University College of Medicine, 103, Daehak-ro, Jongno-gu, Seoul 03080, Korea. Tel: +82.2-2072-2945, Fax: +82.2-762-9662, <email>mdohmd@snu.ac.kr</email></corresp><fn id="afn1" fn-type="equal"><p>*Wan Beom Park and Nak-Jung Kwon equally contributed to the study.</p></fn></author-notes><pub-date pub-type="ppub"><month>2</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>14</day><month>1</month><year>2016</year></pub-date><volume>31</volume><issue>2</issue><fpage>315</fpage><lpage>320</lpage><history><date date-type="received"><day>29</day><month>12</month><year>2015</year></date><date date-type="accepted"><day>11</day><month>1</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 The Korean Academy of Medical Sciences.</copyright-statement><copyright-year>2016</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/4.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/4.0/">http://creativecommons.org/licenses/by-nc/4.0/</ext-link>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p>During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.</p></abstract><kwd-group kwd-group-type="author"><kwd>Middle East Respiratory Syndrome</kwd><kwd>Middle East Respiratory Syndrome Coronavirus</kwd><kwd>Microscopy, Electron</kwd><kwd>Phylogeny</kwd><kwd>Korea</kwd></kwd-group></article-meta></front><floats-group><fig id="F1" fig-type="figure" orientation="portrait" position="float"><label>Fig. 1</label><caption><title>Cytopathic effects of MERS-CoV in Vero cell cultures and Electron microscopy image of MERS-CoV. Vero cells were inoculated with oropharyngeal swab sample. (<bold>A</bold>) Vero cell cultures in negative control. (<bold>B</bold>) Cytopathic effects (rounding and detachment of cells) in Vero cell cultures 3 days after inoculation of the sample. (<bold>C, D</bold>) Transmission electron microscopy image of Vero cells infected with MERS-CoV. White arrow denotes nuclear membrane. Brown scale bar indicates 100 μm (A and B). Black scale bar indicates 500 nm (<bold>C</bold>) and white scale bar does 200 nm (<bold>D</bold>).</title></caption><graphic xlink:href="jkms-31-315-g001"></graphic></fig><fig id="F2" fig-type="figure" orientation="portrait" position="float"><label>Fig. 2</label><caption><title>Molecular phylogenetic analysis. Phylogenetic tree on complete genome (<bold>A</bold>), S genes (<bold>B</bold>), and ORF1ab genes (<bold>C</bold>) for the 101 gene sequences of MERS-CoV. The evolutionary history was inferred by using the maximum likelihood method based on the Tamura-Nei model (<xref rid="B16" ref-type="bibr">16</xref>). Evolutionary analyses were conducted in MEGA6 (<xref rid="B17" ref-type="bibr">17</xref>). Red box indicates our virus isolate.</title></caption><graphic xlink:href="jkms-31-315-g002"></graphic></fig><table-wrap id="T1" orientation="portrait" position="float"><label>Table 1</label><caption><title>PCR primer pairs used in this study</title></caption><alternatives><graphic xlink:href="jkms-31-315-i001"></graphic><table frame="hsides" rules="rows"><thead><tr><th valign="top" align="left" rowspan="1" colspan="1">No.</th><th valign="top" align="center" rowspan="1" colspan="1">Forward</th><th valign="top" align="center" rowspan="1" colspan="1">sequence (5'-->3')</th><th valign="top" align="center" rowspan="1" colspan="1">Reverse</th><th valign="top" align="center" rowspan="1" colspan="1">sequence (5'-->3')</th></tr></thead><tbody><tr><td valign="top" align="right" rowspan="1" colspan="1">1</td><td valign="top" align="left" rowspan="1" colspan="1">FEP_1_F</td><td valign="top" align="left" rowspan="1" colspan="1">GATTTAAGTGAATAGCTTGGCTATCTC</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_70342_1_R</td><td valign="top" align="left" rowspan="1" colspan="1">GGAATATTAGAGACTCCCTGCCG</td></tr><tr><td valign="top" align="right" rowspan="1" colspan="1">2</td><td valign="top" align="left" rowspan="1" colspan="1">2497F</td><td valign="top" align="left" rowspan="1" colspan="1">TCCCATCGGGAACCTATTACTGTG</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68466_R</td><td valign="top" align="left" rowspan="1" colspan="1">TGTAACCACCATTAGTGCGGAC</td></tr><tr><td valign="top" align="right" rowspan="1" colspan="1">3</td><td valign="top" align="left" rowspan="1" colspan="1">4477F</td><td valign="top" align="left" rowspan="1" colspan="1">ACGTTAAGTTAAACCCTTCAGAAG</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_67825_1_R</td><td valign="top" align="left" rowspan="1" colspan="1">AATGAAGCCCTAAATAGTAACTTCACT</td></tr><tr><td valign="top" align="right" rowspan="2" colspan="1">4</td><td valign="top" align="left" rowspan="1" colspan="1">new2_15F_6427</td><td valign="top" align="left" rowspan="1" colspan="1">TGCTTAGATTGCACACCGTT</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68526_R</td><td valign="top" align="left" rowspan="1" colspan="1">AGGTGGTTAACCGGAAAGCTAAA</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">6506F</td><td valign="top" align="left" rowspan="1" colspan="1">AGAATTTGCTACCCGCACTTTCACTG</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68526_R</td><td valign="top" align="left" rowspan="1" colspan="1">AGGTGGTTAACCGGAAAGCTAAA</td></tr><tr><td valign="top" align="right" rowspan="2" colspan="1">5</td><td valign="top" align="left" rowspan="1" colspan="1">new2_19F_8393</td><td valign="top" align="left" rowspan="1" colspan="1">GGATGCACTTAAACGACAGA</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_33984_R</td><td valign="top" align="left" rowspan="1" colspan="1">GTGTAACCAACACTACCACAAGAAC</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">8496F</td><td valign="top" align="left" rowspan="1" colspan="1">TGGTGCTCCTACATGGTTTAATGCG</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_33984_R</td><td valign="top" align="left" rowspan="1" colspan="1">GTGTAACCAACACTACCACAAGAAC</td></tr><tr><td valign="top" align="right" rowspan="1" colspan="1">6</td><td valign="top" align="left" rowspan="1" colspan="1">10477F</td><td valign="top" align="left" rowspan="1" colspan="1">ACACCAAGGAGGGTAGTGTGATC</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_67887_R</td><td valign="top" align="left" rowspan="1" colspan="1">GAATTACAACGCGAAGTTTATTTGAAG</td></tr><tr><td valign="top" align="right" rowspan="2" colspan="1">7</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_2977_F</td><td valign="top" align="left" rowspan="1" colspan="1">AAGGCTTTGCAGAAGGCTGTTA</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68115_R</td><td valign="top" align="left" rowspan="1" colspan="1">GAATTACAACGCGAAGTTTATTTGAAG</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">12488F</td><td valign="top" align="left" rowspan="1" colspan="1">AGGTAGTCACATATCCCTCGCTTAAC</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_68115_R</td><td valign="top" align="left" rowspan="1" colspan="1">TCATCAACTCCTTAAGAGAGAGCCTAT</td></tr><tr><td valign="top" align="right" rowspan="1" colspan="1">8</td><td valign="top" align="left" rowspan="1" colspan="1">14481F</td><td valign="top" align="left" rowspan="1" colspan="1">TGGATGTTAGTCTCCATAGACATAG</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_34102_R</td><td valign="top" align="left" rowspan="1" colspan="1">TGTAATCACCACTTTCAGTCCAGT</td></tr><tr><td valign="top" align="right" rowspan="1" colspan="1">9</td><td valign="top" align="left" rowspan="1" colspan="1">16476F</td><td valign="top" align="left" rowspan="1" colspan="1">TCGGCTTATACAAGAATATGTGCAC</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_981_R</td><td valign="top" align="left" rowspan="1" colspan="1">CATGAGCCCAACAAACAAACGTA</td></tr><tr><td valign="top" align="right" rowspan="1" colspan="1">10</td><td valign="top" align="left" rowspan="1" colspan="1">18490F</td><td valign="top" align="left" rowspan="1" colspan="1">ACGACGTATAGTGCAAATGTTGTC</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_69145_R</td><td valign="top" align="left" rowspan="1" colspan="1">AGCTTTAAATCTATAACAGAACACACC</td></tr><tr><td valign="top" align="right" rowspan="2" colspan="1">11</td><td valign="top" align="left" rowspan="1" colspan="1">new2_42F_20357</td><td valign="top" align="left" rowspan="1" colspan="1">AAGAAGCAACAGGAAGGTCA</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_34878_R</td><td valign="top" align="left" rowspan="1" colspan="1">TAGAAGGCAGCCCAAGCTTTT</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">20490F</td><td valign="top" align="left" rowspan="1" colspan="1">AAGACCTTGGCGTAGTATCCAAGG</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_34878_R</td><td valign="top" align="left" rowspan="1" colspan="1">TAGAAGGCAGCCCAAGCTTTT</td></tr><tr><td valign="top" align="right" rowspan="2" colspan="1">12</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_67088_1_F</td><td valign="top" align="left" rowspan="1" colspan="1">CCACCTTGCCTGTTTATGATACTATTA</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_72579_R</td><td valign="top" align="left" rowspan="1" colspan="1">CTGTTTGCATAGCTCCCAGAG</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">22481F</td><td valign="top" align="left" rowspan="1" colspan="1">TGATTTGTCACAACTCCACTGC</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_72579_R</td><td valign="top" align="left" rowspan="1" colspan="1">CTGTTTGCATAGCTCCCAGAG</td></tr><tr><td valign="top" align="right" rowspan="2" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_66781_F</td><td valign="top" align="left" rowspan="1" colspan="1">TGGACTGCTGGCTTATCCTC</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_66820_R</td><td valign="top" align="left" rowspan="1" colspan="1">GCTTAAATCTATGTATGTTAGCACAGT</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">24512F</td><td valign="top" align="left" rowspan="1" colspan="1">TCAGAAGGTTCAGGATGCTGTGAAC</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_66820_R</td><td valign="top" align="left" rowspan="1" colspan="1">GCTTAAATCTATGTATGTTAGCACAGT</td></tr><tr><td valign="top" align="right" rowspan="1" colspan="1">14</td><td valign="top" align="left" rowspan="1" colspan="1">26470F</td><td valign="top" align="left" rowspan="1" colspan="1">TGAGTTCGCCTTGCTGCGCAAAAC</td><td valign="top" align="left" rowspan="1" colspan="1">FCO_TM58_69858_R</td><td valign="top" align="left" rowspan="1" colspan="1">TGTAATTACCTGCCTTATATCTATGGT</td></tr><tr><td valign="top" align="right" rowspan="2" colspan="1">15</td><td valign="top" align="left" rowspan="1" colspan="1">new2_59F_28427</td><td valign="top" align="left" rowspan="1" colspan="1">GGCAAAGCTACGGAACTAAT</td><td valign="top" align="left" rowspan="1" colspan="1">FEP_3_R</td><td valign="top" align="left" rowspan="1" colspan="1">GCAAATCATCTAATTAGCCTAATCTAATTG</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">28490F</td><td valign="top" align="left" rowspan="1" colspan="1">AACTTGCATTGCTTCGAGCTTAGG</td><td valign="top" align="left" rowspan="1" colspan="1">FEP_3_R</td><td valign="top" align="left" rowspan="1" colspan="1">GCAAATCATCTAATTAGCCTAATCTAATTG</td></tr></tbody></table></alternatives></table-wrap></floats-group></pmc><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Park, Wan Beom" sort="Park, Wan Beom" uniqKey="Park W" first="Wan Beom" last="Park">Wan Beom Park</name></region><name sortKey="Bang, Ji Hwan" sort="Bang, Ji Hwan" uniqKey="Bang J" first="Ji Hwan" last="Bang">Ji Hwan Bang</name><name sortKey="Choe, Pyoeng Gyun" sort="Choe, Pyoeng Gyun" uniqKey="Choe P" first="Pyoeng Gyun" last="Choe">Pyoeng Gyun Choe</name><name sortKey="Choi, Su Jin" sort="Choi, Su Jin" uniqKey="Choi S" first="Su-Jin" last="Choi">Su-Jin Choi</name><name sortKey="Chong, Hyonyong" sort="Chong, Hyonyong" uniqKey="Chong H" first="Hyonyong" last="Chong">Hyonyong Chong</name><name sortKey="Kim, Eu Suk" sort="Kim, Eu Suk" uniqKey="Kim E" first="Eu Suk" last="Kim">Eu Suk Kim</name><name sortKey="Kim, Hong Bin" sort="Kim, Hong Bin" uniqKey="Kim H" first="Hong-Bin" last="Kim">Hong-Bin Kim</name><name sortKey="Kim, Jong Il" sort="Kim, Jong Il" uniqKey="Kim J" first="Jong-Il" last="Kim">Jong-Il Kim</name><name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name><name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name><name sortKey="Kwon, Nak Jung" sort="Kwon, Nak Jung" uniqKey="Kwon N" first="Nak-Jung" last="Kwon">Nak-Jung Kwon</name><name sortKey="Lee, Sang Min" sort="Lee, Sang Min" uniqKey="Lee S" first="Sang Min" last="Lee">Sang Min Lee</name><name sortKey="Oh, Hong Sang" sort="Oh, Hong Sang" uniqKey="Oh H" first="Hong Sang" last="Oh">Hong Sang Oh</name><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung-Don" last="Oh">Myoung-Don Oh</name><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung-Don" last="Oh">Myoung-Don Oh</name><name sortKey="Park, Sang Won" sort="Park, Sang Won" uniqKey="Park S" first="Sang Won" last="Park">Sang Won Park</name><name sortKey="Park, Wan Beom" sort="Park, Wan Beom" uniqKey="Park W" first="Wan Beom" last="Park">Wan Beom Park</name><name sortKey="Song, Kyoung Ho" sort="Song, Kyoung Ho" uniqKey="Song K" first="Kyoung-Ho" last="Song">Kyoung-Ho Song</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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