Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic human DPP4 mouse model
Identifieur interne : 000741 ( Pmc/Checkpoint ); précédent : 000740; suivant : 000742Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic human DPP4 mouse model
Auteurs : Vincent J. Munster [États-Unis] ; Daniel Wells [Royaume-Uni] ; Teresa Lambe [Royaume-Uni] ; Daniel Wright [Royaume-Uni] ; Robert J. Fischer [États-Unis] ; Trenton Bushmaker [États-Unis] ; Greg Saturday [États-Unis] ; Neeltje Van Doremalen [États-Unis] ; Sarah C. Gilbert [Royaume-Uni] ; Emmie De Wit [États-Unis] ; George M. Warimwe [Royaume-Uni, Kenya]Source :
- NPJ Vaccines [ 2059-0105 ] ; 2017.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel zoonotic virus that causes severe respiratory disease in humans with a case fatality rate close to 40%, but for which no vaccines are available. Here, we evaluated the utility of ChAdOx1, a promising replication-deficient simian adenovirus vaccine vector platform with an established safety profile in humans and dromedary camels, for MERS-CoV vaccine development. Using a transgenic lethal BALB/c MERS-CoV mouse model we showed that single dose intranasal or intramuscular immunisation with ChAdOx1 MERS, encoding full-length MERS-CoV Spike glycoprotein, is highly immunogenic and confers protection against lethal viral challenge. Immunogenicity and efficacy were comparable between immunisation routes. Together these data provide support for further evaluation of ChAdOx1 MERS vaccine in humans and dromedary camels, the animal reservoir of infection.
Url:
DOI: 10.1038/s41541-017-0029-1
PubMed: 29263883
PubMed Central: 5643297
Affiliations:
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PMC:5643297Le document en format XML
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<front><div type="abstract" xml:lang="en"><p id="Par1">Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel zoonotic virus that causes severe respiratory disease in humans with a case fatality rate close to 40%, but for which no vaccines are available. Here, we evaluated the utility of ChAdOx1, a promising replication-deficient simian adenovirus vaccine vector platform with an established safety profile in humans and dromedary camels, for MERS-CoV vaccine development. Using a transgenic lethal BALB/c MERS-CoV mouse model we showed that single dose intranasal or intramuscular immunisation with ChAdOx1 MERS, encoding full-length MERS-CoV Spike glycoprotein, is highly immunogenic and confers protection against lethal viral challenge. Immunogenicity and efficacy were comparable between immunisation routes. Together these data provide support for further evaluation of ChAdOx1 MERS vaccine in humans and dromedary camels, the animal reservoir of infection.</p>
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<biblStruct><analytic><author><name sortKey="De Wit, E" uniqKey="De Wit E">E de Wit</name>
</author>
</analytic>
</biblStruct>
</listBibl>
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</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">NPJ Vaccines</journal-id>
<journal-id journal-id-type="iso-abbrev">NPJ Vaccines</journal-id>
<journal-title-group><journal-title>NPJ Vaccines</journal-title>
</journal-title-group>
<issn pub-type="epub">2059-0105</issn>
<publisher><publisher-name>Nature Publishing Group UK</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">29263883</article-id>
<article-id pub-id-type="pmc">5643297</article-id>
<article-id pub-id-type="publisher-id">29</article-id>
<article-id pub-id-type="doi">10.1038/s41541-017-0029-1</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Brief Communication</subject>
</subj-group>
</article-categories>
<title-group><article-title>Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic human DPP4 mouse model</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" corresp="yes" equal-contrib="yes"><name><surname>Munster</surname>
<given-names>Vincent J.</given-names>
</name>
<address><email>vincent.munster@nih.gov</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-2007-8978</contrib-id>
<name><surname>Wells</surname>
<given-names>Daniel</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lambe</surname>
<given-names>Teresa</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wright</surname>
<given-names>Daniel</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fischer</surname>
<given-names>Robert J.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bushmaker</surname>
<given-names>Trenton</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Saturday</surname>
<given-names>Greg</given-names>
</name>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0003-4368-6359</contrib-id>
<name><surname>van Doremalen</surname>
<given-names>Neeltje</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gilbert</surname>
<given-names>Sarah C.</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-9763-7758</contrib-id>
<name><surname>de Wit</surname>
<given-names>Emmie</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Warimwe</surname>
<given-names>George M.</given-names>
</name>
<address><email>george.warimwe@ndm.ox.ac.uk</email>
</address>
<xref ref-type="aff" rid="Aff2">2</xref>
<xref ref-type="aff" rid="Aff4">4</xref>
<xref ref-type="aff" rid="Aff5">5</xref>
</contrib>
<aff id="Aff1"><label>1</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id>
<institution-id institution-id-type="GRID">grid.419681.3</institution-id>
<institution>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution>
<institution>Rocky Mountain Laboratories,</institution>
</institution-wrap>
Hamilton, MT USA</aff>
<aff id="Aff2"><label>2</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 8948</institution-id>
<institution-id institution-id-type="GRID">grid.4991.5</institution-id>
<institution>The Jenner Institute, University of Oxford,</institution>
</institution-wrap>
Oxford, UK</aff>
<aff id="Aff3"><label>3</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2297 5165</institution-id>
<institution-id institution-id-type="GRID">grid.94365.3d</institution-id>
<institution>Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases,</institution>
<institution>National Institutes of Health,</institution>
</institution-wrap>
Hamilton, MT USA</aff>
<aff id="Aff4"><label>4</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0388 7540</institution-id>
<institution-id institution-id-type="GRID">grid.63622.33</institution-id>
<institution>The Pirbright Institute,</institution>
</institution-wrap>
Woking, UK</aff>
<aff id="Aff5"><label>5</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0155 5938</institution-id>
<institution-id institution-id-type="GRID">grid.33058.3d</institution-id>
<institution>KEMRI-Wellcome Trust Research Programme,</institution>
</institution-wrap>
Kilifi, Kenya</aff>
</contrib-group>
<pub-date pub-type="epub"><day>16</day>
<month>10</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>16</day>
<month>10</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="collection"><year>2017</year>
</pub-date>
<volume>2</volume>
<elocation-id>28</elocation-id>
<history><date date-type="received"><day>20</day>
<month>3</month>
<year>2017</year>
</date>
<date date-type="rev-recd"><day>2</day>
<month>9</month>
<year>2017</year>
</date>
<date date-type="accepted"><day>15</day>
<month>9</month>
<year>2017</year>
</date>
</history>
<permissions><copyright-statement>© The Author(s) 2017</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
.</license-p>
</license>
</permissions>
<abstract id="Abs1"><p id="Par1">Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel zoonotic virus that causes severe respiratory disease in humans with a case fatality rate close to 40%, but for which no vaccines are available. Here, we evaluated the utility of ChAdOx1, a promising replication-deficient simian adenovirus vaccine vector platform with an established safety profile in humans and dromedary camels, for MERS-CoV vaccine development. Using a transgenic lethal BALB/c MERS-CoV mouse model we showed that single dose intranasal or intramuscular immunisation with ChAdOx1 MERS, encoding full-length MERS-CoV Spike glycoprotein, is highly immunogenic and confers protection against lethal viral challenge. Immunogenicity and efficacy were comparable between immunisation routes. Together these data provide support for further evaluation of ChAdOx1 MERS vaccine in humans and dromedary camels, the animal reservoir of infection.</p>
</abstract>
<kwd-group kwd-group-type="npg-subject"><title>Subject terms</title>
<kwd>Vaccines</kwd>
<kwd>Vaccines</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2017</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Kenya</li>
<li>Royaume-Uni</li>
<li>États-Unis</li>
</country>
<region><li>Montana</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Montana"><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J." last="Munster">Vincent J. Munster</name>
</region>
<name sortKey="Bushmaker, Trenton" sort="Bushmaker, Trenton" uniqKey="Bushmaker T" first="Trenton" last="Bushmaker">Trenton Bushmaker</name>
<name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name>
<name sortKey="Fischer, Robert J" sort="Fischer, Robert J" uniqKey="Fischer R" first="Robert J." last="Fischer">Robert J. Fischer</name>
<name sortKey="Saturday, Greg" sort="Saturday, Greg" uniqKey="Saturday G" first="Greg" last="Saturday">Greg Saturday</name>
<name sortKey="Van Doremalen, Neeltje" sort="Van Doremalen, Neeltje" uniqKey="Van Doremalen N" first="Neeltje" last="Van Doremalen">Neeltje Van Doremalen</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Wells, Daniel" sort="Wells, Daniel" uniqKey="Wells D" first="Daniel" last="Wells">Daniel Wells</name>
</noRegion>
<name sortKey="Gilbert, Sarah C" sort="Gilbert, Sarah C" uniqKey="Gilbert S" first="Sarah C." last="Gilbert">Sarah C. Gilbert</name>
<name sortKey="Lambe, Teresa" sort="Lambe, Teresa" uniqKey="Lambe T" first="Teresa" last="Lambe">Teresa Lambe</name>
<name sortKey="Warimwe, George M" sort="Warimwe, George M" uniqKey="Warimwe G" first="George M." last="Warimwe">George M. Warimwe</name>
<name sortKey="Warimwe, George M" sort="Warimwe, George M" uniqKey="Warimwe G" first="George M." last="Warimwe">George M. Warimwe</name>
<name sortKey="Wright, Daniel" sort="Wright, Daniel" uniqKey="Wright D" first="Daniel" last="Wright">Daniel Wright</name>
</country>
<country name="Kenya"><noRegion><name sortKey="Warimwe, George M" sort="Warimwe, George M" uniqKey="Warimwe G" first="George M." last="Warimwe">George M. Warimwe</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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