Prophylactic and therapeutic efficacy of mAb treatment against MERS-CoV in common marmosets
Identifieur interne : 000491 ( Pmc/Checkpoint ); précédent : 000490; suivant : 000492Prophylactic and therapeutic efficacy of mAb treatment against MERS-CoV in common marmosets
Auteurs : Emmie De Wit [États-Unis] ; Friederike Feldmann [États-Unis] ; Atsushi Okumura [États-Unis] ; Eva Horne [États-Unis] ; Elaine Haddock [États-Unis] ; Greg Saturday [États-Unis] ; Dana Scott [États-Unis] ; Karl J. Erlandson [États-Unis] ; Neil Stahl [États-Unis] ; Leah Lipsich [États-Unis] ; Christos A. Kyratsous [États-Unis] ; Heinz Feldmann [États-Unis]Source :
- Antiviral Research [ 0166-3542 ] ; 2018.
Abstract
The high case-fatality rate of confirmed MERS-CoV infections underlines the urgent need for an effective treatment to reduce the disease severity and mortality. REGN3051 and REGN3048 are two fully human neutralizing monoclonal antibodies (mAb) against MERS-CoV that reduced virus replication in mice expressing human DPP4 upon prophylactic and therapeutic treatment. Here, we evaluated the prophylactic and therapeutic efficacy of REGN3048 and REGN3051 in the common marmoset model of MERS-CoV infection. Intravenous administration of mAb resulted in high levels of MERS-CoV-neutralizing activity in circulating blood. When animals were treated with mAbs one day before challenge, respiratory disease was less severe and, in animals treated with both REGN3048 and REGN3051, viral loads in the lungs were reduced. However, therapeutic treatment on day one after challenge was less efficacious as it did not prevent the development of severe respiratory disease and all treated animals developed bronchointerstitial pneumonia of similar severity as the control animals. Thus, mAb administration may be more effective in a prophylactic treatment regimen rather than treatment of MERS.
Url:
DOI: 10.1016/j.antiviral.2018.06.006
PubMed: 29885377
PubMed Central: 7113689
Affiliations:
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last="De Wit">Emmie De Wit</name><affiliation wicri:level="2"><nlm:aff id="aff1">Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name><affiliation wicri:level="2"><nlm:aff id="aff2">Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name><affiliation wicri:level="4"><nlm:aff id="aff3">Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">New York</settlement></placeName><orgName type="university">Université Columbia</orgName></affiliation></author><author><name sortKey="Horne, Eva" sort="Horne, Eva" uniqKey="Horne E" first="Eva" last="Horne">Eva Horne</name><affiliation wicri:level="2"><nlm:aff id="aff1">Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name><affiliation wicri:level="2"><nlm:aff id="aff1">Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Saturday, Greg" sort="Saturday, Greg" uniqKey="Saturday G" first="Greg" last="Saturday">Greg Saturday</name><affiliation wicri:level="2"><nlm:aff id="aff2">Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name><affiliation wicri:level="2"><nlm:aff id="aff2">Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Erlandson, Karl J" sort="Erlandson, Karl J" uniqKey="Erlandson K" first="Karl J." last="Erlandson">Karl J. Erlandson</name><affiliation wicri:level="2"><nlm:aff id="aff4">Biomedical Advanced Research and Development Authority, Washington, DC, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biomedical Advanced Research and Development Authority, Washington, DC</wicri:regionArea><placeName><region type="state">District de Columbia</region></placeName></affiliation></author><author><name sortKey="Stahl, Neil" sort="Stahl, Neil" uniqKey="Stahl N" first="Neil" last="Stahl">Neil Stahl</name><affiliation wicri:level="2"><nlm:aff id="aff5">Regeneron Pharmaceuticals, Inc., Tarrytown, NY, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Regeneron Pharmaceuticals, Inc., Tarrytown, NY</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Lipsich, Leah" sort="Lipsich, Leah" uniqKey="Lipsich L" first="Leah" last="Lipsich">Leah Lipsich</name><affiliation wicri:level="2"><nlm:aff id="aff5">Regeneron Pharmaceuticals, Inc., Tarrytown, NY, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Regeneron Pharmaceuticals, Inc., Tarrytown, NY</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Kyratsous, Christos A" sort="Kyratsous, Christos A" uniqKey="Kyratsous C" first="Christos A." last="Kyratsous">Christos A. Kyratsous</name><affiliation wicri:level="2"><nlm:aff id="aff5">Regeneron Pharmaceuticals, Inc., Tarrytown, NY, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Regeneron Pharmaceuticals, Inc., Tarrytown, NY</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><affiliation wicri:level="2"><nlm:aff id="aff1">Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author></analytic><series><title level="j">Antiviral Research</title><idno type="ISSN">0166-3542</idno><idno type="eISSN">1872-9096</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The high case-fatality rate of confirmed MERS-CoV infections underlines the urgent need for an effective treatment to reduce the disease severity and mortality. REGN3051 and REGN3048 are two fully human neutralizing monoclonal antibodies (mAb) against MERS-CoV that reduced virus replication in mice expressing human DPP4 upon prophylactic and therapeutic treatment. Here, we evaluated the prophylactic and therapeutic efficacy of REGN3048 and REGN3051 in the common marmoset model of MERS-CoV infection. Intravenous administration of mAb resulted in high levels of MERS-CoV-neutralizing activity in circulating blood. When animals were treated with mAbs one day before challenge, respiratory disease was less severe and, in animals treated with both REGN3048 and REGN3051, viral loads in the lungs were reduced. However, therapeutic treatment on day one after challenge was less efficacious as it did not prevent the development of severe respiratory disease and all treated animals developed bronchointerstitial pneumonia of similar severity as the control animals. Thus, mAb administration may be more effective in a prophylactic treatment regimen rather than treatment of MERS.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Agrawal, A S" uniqKey="Agrawal A">A.S. Agrawal</name></author><author><name sortKey="Ying, T" uniqKey="Ying T">T. Ying</name></author><author><name sortKey="Tao, X" uniqKey="Tao X">X. Tao</name></author><author><name sortKey="Garron, T" uniqKey="Garron T">T. Garron</name></author><author><name sortKey="Algaissi, A" uniqKey="Algaissi A">A. Algaissi</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Peng, B H" uniqKey="Peng B">B.H. Peng</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name></author><author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name></author><author><name sortKey="Tseng, C T" uniqKey="Tseng C">C.T. Tseng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="American Academy Of Pediatrics Committee On Infectious D" uniqKey="American Academy Of Pediatrics Committee On Infectious D">American Academy of Pediatrics Committee on Infectious, D</name></author><author><name sortKey="American Academy Of Pediatrics Bronchiolitis Guidelines C" uniqKey="American Academy Of Pediatrics Bronchiolitis Guidelines C">American Academy of Pediatrics Bronchiolitis Guidelines, C</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arabi, Y M" uniqKey="Arabi Y">Y.M. Arabi</name></author><author><name sortKey="Hajeer, A H" uniqKey="Hajeer A">A.H. Hajeer</name></author><author><name sortKey="Luke, T" uniqKey="Luke T">T. Luke</name></author><author><name sortKey="Raviprakash, K" uniqKey="Raviprakash K">K. Raviprakash</name></author><author><name sortKey="Balkhy, H" uniqKey="Balkhy H">H. Balkhy</name></author><author><name sortKey="Johani, S" uniqKey="Johani S">S. Johani</name></author><author><name sortKey="Al Dawood, A" uniqKey="Al Dawood A">A. Al-Dawood</name></author><author><name sortKey="Al Qahtani, S" uniqKey="Al Qahtani S">S. Al-Qahtani</name></author><author><name sortKey="Al Omari, A" uniqKey="Al Omari A">A. Al-Omari</name></author><author><name sortKey="Al Hameed, F" uniqKey="Al Hameed F">F. Al-Hameed</name></author><author><name sortKey="Hayden, F G" uniqKey="Hayden F">F.G. Hayden</name></author><author><name sortKey="Fowler, R" uniqKey="Fowler R">R. Fowler</name></author><author><name sortKey="Bouchama, A" uniqKey="Bouchama A">A. Bouchama</name></author><author><name sortKey="Shindo, N" uniqKey="Shindo N">N. Shindo</name></author><author><name sortKey="Al Khairy, K" uniqKey="Al Khairy K">K. Al-Khairy</name></author><author><name sortKey="Carson, G" uniqKey="Carson G">G. Carson</name></author><author><name sortKey="Taha, Y" uniqKey="Taha Y">Y. Taha</name></author><author><name sortKey="Sadat, M" uniqKey="Sadat M">M. Sadat</name></author><author><name sortKey="Alahmadi, M" uniqKey="Alahmadi M">M. Alahmadi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baseler, L J" uniqKey="Baseler L">L.J. Baseler</name></author><author><name sortKey="Falzarano, D" uniqKey="Falzarano D">D. Falzarano</name></author><author><name sortKey="Scott, D P" uniqKey="Scott D">D.P. Scott</name></author><author><name sortKey="Rosenke, R" uniqKey="Rosenke R">R. Rosenke</name></author><author><name sortKey="Thomas, T" uniqKey="Thomas T">T. Thomas</name></author><author><name sortKey="Munster, V J" uniqKey="Munster V">V.J. Munster</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E. de Wit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, J F" uniqKey="Chan J">J.F. Chan</name></author><author><name sortKey="Yao, Y" uniqKey="Yao Y">Y. Yao</name></author><author><name sortKey="Yeung, M L" uniqKey="Yeung M">M.L. Yeung</name></author><author><name sortKey="Deng, W" uniqKey="Deng W">W. Deng</name></author><author><name sortKey="Bao, L" uniqKey="Bao L">L. Bao</name></author><author><name sortKey="Jia, L" uniqKey="Jia L">L. Jia</name></author><author><name sortKey="Li, F" uniqKey="Li F">F. Li</name></author><author><name sortKey="Xiao, C" uniqKey="Xiao C">C. Xiao</name></author><author><name sortKey="Gao, H" uniqKey="Gao H">H. Gao</name></author><author><name sortKey="Yu, P" uniqKey="Yu P">P. Yu</name></author><author><name sortKey="Cai, J P" uniqKey="Cai J">J.P. Cai</name></author><author><name sortKey="Chu, H" uniqKey="Chu H">H. Chu</name></author><author><name sortKey="Zhou, J" uniqKey="Zhou J">J. Zhou</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name></author><author><name sortKey="Qin, C" uniqKey="Qin C">C. Qin</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Bao, L" uniqKey="Bao L">L. Bao</name></author><author><name sortKey="Chen, C" uniqKey="Chen C">C. Chen</name></author><author><name sortKey="Zou, T" uniqKey="Zou T">T. Zou</name></author><author><name sortKey="Xue, Y" uniqKey="Xue Y">Y. Xue</name></author><author><name sortKey="Li, F" uniqKey="Li F">F. Li</name></author><author><name sortKey="Lv, Q" uniqKey="Lv Q">Q. Lv</name></author><author><name sortKey="Gu, S" uniqKey="Gu S">S. Gu</name></author><author><name sortKey="Gao, X" uniqKey="Gao X">X. Gao</name></author><author><name sortKey="Cui, S" uniqKey="Cui S">S. Cui</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Qin, C" uniqKey="Qin C">C. Qin</name></author><author><name sortKey="Jin, Q" uniqKey="Jin Q">Q. Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Consortium I S A R E I" uniqKey="Consortium I S A R E I">Consortium, I.S.A.R.E.I.</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Eckerle, I" uniqKey="Eckerle I">I. Eckerle</name></author><author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T. Bleicker</name></author><author><name sortKey="Zaki, A" uniqKey="Zaki A">A. Zaki</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O. Landt</name></author><author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M. Eschbach-Bludau</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="Gopal, R" uniqKey="Gopal R">R. Gopal</name></author><author><name sortKey="Ballhause, M" uniqKey="Ballhause M">M. Ballhause</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Muth, D" uniqKey="Muth D">D. Muth</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Drexler, J F" uniqKey="Drexler J">J.F. Drexler</name></author><author><name sortKey="Zambon, M" uniqKey="Zambon M">M. Zambon</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Fouchier, R M" uniqKey="Fouchier R">R.M. Fouchier</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corti, D" uniqKey="Corti D">D. Corti</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author><author><name sortKey="Pedotti, M" uniqKey="Pedotti M">M. Pedotti</name></author><author><name sortKey="Simonelli, L" uniqKey="Simonelli L">L. Simonelli</name></author><author><name sortKey="Agnihothram, S" uniqKey="Agnihothram S">S. Agnihothram</name></author><author><name sortKey="Fett, C" uniqKey="Fett C">C. Fett</name></author><author><name sortKey="Fernandez Rodriguez, B" uniqKey="Fernandez Rodriguez B">B. Fernandez-Rodriguez</name></author><author><name sortKey="Foglierini, M" uniqKey="Foglierini M">M. Foglierini</name></author><author><name sortKey="Agatic, G" uniqKey="Agatic G">G. Agatic</name></author><author><name sortKey="Vanzetta, F" uniqKey="Vanzetta F">F. Vanzetta</name></author><author><name sortKey="Gopal, R" uniqKey="Gopal R">R. Gopal</name></author><author><name sortKey="Langrish, C J" uniqKey="Langrish C">C.J. Langrish</name></author><author><name sortKey="Barrett, N A" uniqKey="Barrett N">N.A. Barrett</name></author><author><name sortKey="Sallusto, F" uniqKey="Sallusto F">F. Sallusto</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Varani, L" uniqKey="Varani L">L. Varani</name></author><author><name sortKey="Zambon, M" uniqKey="Zambon M">M. Zambon</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author><author><name sortKey="Lanzavecchia, A" uniqKey="Lanzavecchia A">A. Lanzavecchia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dall Acqua, W F" uniqKey="Dall Acqua W">W.F. Dall'Acqua</name></author><author><name sortKey="Kiener, P A" uniqKey="Kiener P">P.A. Kiener</name></author><author><name sortKey="Wu, H" uniqKey="Wu H">H. Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Falzarano, D" uniqKey="Falzarano D">D. Falzarano</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E. de Wit</name></author><author><name sortKey="Feldmann, F" uniqKey="Feldmann F">F. Feldmann</name></author><author><name sortKey="Rasmussen, A L" uniqKey="Rasmussen A">A.L. Rasmussen</name></author><author><name sortKey="Okumura, A" uniqKey="Okumura A">A. Okumura</name></author><author><name sortKey="Peng, X" uniqKey="Peng X">X. Peng</name></author><author><name sortKey="Thomas, M J" uniqKey="Thomas M">M.J. Thomas</name></author><author><name sortKey="Van Doremalen, N" uniqKey="Van Doremalen N">N. van Doremalen</name></author><author><name sortKey="Haddock, E" uniqKey="Haddock E">E. Haddock</name></author><author><name sortKey="Nagy, L" uniqKey="Nagy L">L. Nagy</name></author><author><name sortKey="Lacasse, R" uniqKey="Lacasse R">R. LaCasse</name></author><author><name sortKey="Liu, T" uniqKey="Liu T">T. Liu</name></author><author><name sortKey="Zhu, J" uniqKey="Zhu J">J. Zhu</name></author><author><name sortKey="Mclellan, J S" uniqKey="Mclellan J">J.S. McLellan</name></author><author><name sortKey="Scott, D P" uniqKey="Scott D">D.P. Scott</name></author><author><name sortKey="Katze, M G" uniqKey="Katze M">M.G. Katze</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name></author><author><name sortKey="Munster, V J" uniqKey="Munster V">V.J. Munster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hart, T K" uniqKey="Hart T">T.K. Hart</name></author><author><name sortKey="Cook, R M" uniqKey="Cook R">R.M. Cook</name></author><author><name sortKey="Zia Amirhosseini, P" uniqKey="Zia Amirhosseini P">P. Zia-Amirhosseini</name></author><author><name sortKey="Minthorn, E" uniqKey="Minthorn E">E. Minthorn</name></author><author><name sortKey="Sellers, T S" uniqKey="Sellers T">T.S. Sellers</name></author><author><name sortKey="Maleeff, B E" uniqKey="Maleeff B">B.E. Maleeff</name></author><author><name sortKey="Eustis, S" uniqKey="Eustis S">S. Eustis</name></author><author><name sortKey="Schwartz, L W" uniqKey="Schwartz L">L.W. Schwartz</name></author><author><name sortKey="Tsui, P" uniqKey="Tsui P">P. Tsui</name></author><author><name sortKey="Appelbaum, E R" uniqKey="Appelbaum E">E.R. Appelbaum</name></author><author><name sortKey="Martin, E C" uniqKey="Martin E">E.C. Martin</name></author><author><name sortKey="Bugelski, P J" uniqKey="Bugelski P">P.J. Bugelski</name></author><author><name sortKey="Herzyk, D J" uniqKey="Herzyk D">D.J. Herzyk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Houser, K V" uniqKey="Houser K">K.V. Houser</name></author><author><name sortKey="Gretebeck, L" uniqKey="Gretebeck L">L. Gretebeck</name></author><author><name sortKey="Ying, T" uniqKey="Ying T">T. Ying</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Vogel, L" uniqKey="Vogel L">L. Vogel</name></author><author><name sortKey="Lamirande, E W" uniqKey="Lamirande E">E.W. Lamirande</name></author><author><name sortKey="Bock, K W" uniqKey="Bock K">K.W. Bock</name></author><author><name sortKey="Moore, I N" uniqKey="Moore I">I.N. Moore</name></author><author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K. Subbarao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Johnson, R F" uniqKey="Johnson R">R.F. Johnson</name></author><author><name sortKey="Bagci, U" uniqKey="Bagci U">U. Bagci</name></author><author><name sortKey="Keith, L" uniqKey="Keith L">L. Keith</name></author><author><name sortKey="Tang, X" uniqKey="Tang X">X. Tang</name></author><author><name sortKey="Mollura, D J" uniqKey="Mollura D">D.J. Mollura</name></author><author><name sortKey="Zeitlin, L" uniqKey="Zeitlin L">L. Zeitlin</name></author><author><name sortKey="Qin, J" uniqKey="Qin J">J. Qin</name></author><author><name sortKey="Huzella, L" uniqKey="Huzella L">L. Huzella</name></author><author><name sortKey="Bartos, C J" uniqKey="Bartos C">C.J. Bartos</name></author><author><name sortKey="Bohorova, N" uniqKey="Bohorova N">N. Bohorova</name></author><author><name sortKey="Bohorov, O" uniqKey="Bohorov O">O. Bohorov</name></author><author><name sortKey="Goodman, C" uniqKey="Goodman C">C. Goodman</name></author><author><name sortKey="Kim Do, H" uniqKey="Kim Do H">H. Kim do</name></author><author><name sortKey="Paulty, M H" uniqKey="Paulty M">M.H. Paulty</name></author><author><name sortKey="Velasco, J" uniqKey="Velasco J">J. Velasco</name></author><author><name sortKey="Whaley, K J" uniqKey="Whaley K">K.J. Whaley</name></author><author><name sortKey="Johnson, J C" uniqKey="Johnson J">J.C. Johnson</name></author><author><name sortKey="Pettitt, J" uniqKey="Pettitt J">J. Pettitt</name></author><author><name sortKey="Ork, B L" uniqKey="Ork B">B.L. Ork</name></author><author><name sortKey="Solomon, J" uniqKey="Solomon J">J. Solomon</name></author><author><name sortKey="Oberlander, N" uniqKey="Oberlander N">N. Oberlander</name></author><author><name sortKey="Zhu, Q" uniqKey="Zhu Q">Q. Zhu</name></author><author><name sortKey="Sun, J" uniqKey="Sun J">J. Sun</name></author><author><name sortKey="Holbrook, M R" uniqKey="Holbrook M">M.R. Holbrook</name></author><author><name sortKey="Olinger, G G" uniqKey="Olinger G">G.G. Olinger</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Hensley, L E" uniqKey="Hensley L">L.E. Hensley</name></author><author><name sortKey="Jahrling, P B" uniqKey="Jahrling P">P.B. Jahrling</name></author><author><name sortKey="Marasco, W A" uniqKey="Marasco W">W.A. Marasco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Wan, Y" uniqKey="Wan Y">Y. Wan</name></author><author><name sortKey="Liu, P" uniqKey="Liu P">P. Liu</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author><author><name sortKey="Lu, G" uniqKey="Lu G">G. Lu</name></author><author><name sortKey="Qi, J" uniqKey="Qi J">J. Qi</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X. Lu</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y. Wu</name></author><author><name sortKey="Liu, W" uniqKey="Liu W">W. Liu</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B. Zhang</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author><author><name sortKey="Gao, G F" uniqKey="Gao G">G.F. Gao</name></author><author><name sortKey="Yan, J" uniqKey="Yan J">J. Yan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luke, T" uniqKey="Luke T">T. Luke</name></author><author><name sortKey="Wu, H" uniqKey="Wu H">H. Wu</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author><author><name sortKey="Channappanavar, R" uniqKey="Channappanavar R">R. Channappanavar</name></author><author><name sortKey="Coleman, C M" uniqKey="Coleman C">C.M. Coleman</name></author><author><name sortKey="Jiao, J A" uniqKey="Jiao J">J.A. Jiao</name></author><author><name sortKey="Matsushita, H" uniqKey="Matsushita H">H. Matsushita</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. Liu</name></author><author><name sortKey="Postnikova, E N" uniqKey="Postnikova E">E.N. Postnikova</name></author><author><name sortKey="Ork, B L" uniqKey="Ork B">B.L. Ork</name></author><author><name sortKey="Glenn, G" uniqKey="Glenn G">G. Glenn</name></author><author><name sortKey="Flyer, D" uniqKey="Flyer D">D. Flyer</name></author><author><name sortKey="Defang, G" uniqKey="Defang G">G. Defang</name></author><author><name sortKey="Raviprakash, K" uniqKey="Raviprakash K">K. Raviprakash</name></author><author><name sortKey="Kochel, T" uniqKey="Kochel T">T. Kochel</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Nie, W" uniqKey="Nie W">W. Nie</name></author><author><name sortKey="Smith, G" uniqKey="Smith G">G. Smith</name></author><author><name sortKey="Hensley, L E" uniqKey="Hensley L">L.E. Hensley</name></author><author><name sortKey="Olinger, G G" uniqKey="Olinger G">G.G. Olinger</name></author><author><name sortKey="Kuhn, J H" uniqKey="Kuhn J">J.H. Kuhn</name></author><author><name sortKey="Holbrook, M R" uniqKey="Holbrook M">M.R. Holbrook</name></author><author><name sortKey="Johnson, R F" uniqKey="Johnson R">R.F. Johnson</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author><author><name sortKey="Sullivan, E" uniqKey="Sullivan E">E. Sullivan</name></author><author><name sortKey="Frieman, M B" uniqKey="Frieman M">M.B. Frieman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mair Jenkins, J" uniqKey="Mair Jenkins J">J. Mair-Jenkins</name></author><author><name sortKey="Saavedra Campos, M" uniqKey="Saavedra Campos M">M. Saavedra-Campos</name></author><author><name sortKey="Baillie, J K" uniqKey="Baillie J">J.K. Baillie</name></author><author><name sortKey="Cleary, P" uniqKey="Cleary P">P. Cleary</name></author><author><name sortKey="Khaw, F M" uniqKey="Khaw F">F.M. Khaw</name></author><author><name sortKey="Lim, W S" uniqKey="Lim W">W.S. Lim</name></author><author><name sortKey="Makki, S" uniqKey="Makki S">S. Makki</name></author><author><name sortKey="Rooney, K D" uniqKey="Rooney K">K.D. Rooney</name></author><author><name sortKey="Beck, C R" uniqKey="Beck C">C.R. Beck</name></author><author><name sortKey="Convalescent Plasma Study, G" uniqKey="Convalescent Plasma Study G">G. Convalescent Plasma Study</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pascal, K E" uniqKey="Pascal K">K.E. Pascal</name></author><author><name sortKey="Coleman, C M" uniqKey="Coleman C">C.M. Coleman</name></author><author><name sortKey="Mujica, A O" uniqKey="Mujica A">A.O. Mujica</name></author><author><name sortKey="Kamat, V" uniqKey="Kamat V">V. Kamat</name></author><author><name sortKey="Badithe, A" uniqKey="Badithe A">A. Badithe</name></author><author><name sortKey="Fairhurst, J" uniqKey="Fairhurst J">J. Fairhurst</name></author><author><name sortKey="Hunt, C" uniqKey="Hunt C">C. Hunt</name></author><author><name sortKey="Strein, J" uniqKey="Strein J">J. Strein</name></author><author><name sortKey="Berrebi, A" uniqKey="Berrebi A">A. Berrebi</name></author><author><name sortKey="Sisk, J M" uniqKey="Sisk J">J.M. Sisk</name></author><author><name sortKey="Matthews, K L" uniqKey="Matthews K">K.L. Matthews</name></author><author><name sortKey="Babb, R" uniqKey="Babb R">R. Babb</name></author><author><name sortKey="Chen, G" uniqKey="Chen G">G. Chen</name></author><author><name sortKey="Lai, K M" uniqKey="Lai K">K.M. Lai</name></author><author><name sortKey="Huang, T T" uniqKey="Huang T">T.T. Huang</name></author><author><name sortKey="Olson, W" uniqKey="Olson W">W. Olson</name></author><author><name sortKey="Yancopoulos, G D" uniqKey="Yancopoulos G">G.D. Yancopoulos</name></author><author><name sortKey="Stahl, N" uniqKey="Stahl N">N. Stahl</name></author><author><name sortKey="Frieman, M B" uniqKey="Frieman M">M.B. Frieman</name></author><author><name sortKey="Kyratsous, C A" uniqKey="Kyratsous C">C.A. Kyratsous</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Prescott, J" uniqKey="Prescott J">J. Prescott</name></author><author><name sortKey="Falzarano, D" uniqKey="Falzarano D">D. Falzarano</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E. de Wit</name></author><author><name sortKey="Hardcastle, K" uniqKey="Hardcastle K">K. Hardcastle</name></author><author><name sortKey="Feldmann, F" uniqKey="Feldmann F">F. Feldmann</name></author><author><name sortKey="Haddock, E" uniqKey="Haddock E">E. Haddock</name></author><author><name sortKey="Scott, D" uniqKey="Scott D">D. Scott</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name></author><author><name sortKey="Munster, V J" uniqKey="Munster V">V.J. Munster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, H" uniqKey="Qiu H">H. Qiu</name></author><author><name sortKey="Sun, S" uniqKey="Sun S">S. Sun</name></author><author><name sortKey="Xiao, H" uniqKey="Xiao H">H. Xiao</name></author><author><name sortKey="Feng, J" uniqKey="Feng J">J. Feng</name></author><author><name sortKey="Guo, Y" uniqKey="Guo Y">Y. Guo</name></author><author><name sortKey="Tai, W" uniqKey="Tai W">W. Tai</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Du, L" uniqKey="Du L">L. Du</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G. Zhao</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y. Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Respaud, R" uniqKey="Respaud R">R. Respaud</name></author><author><name sortKey="Vecellio, L" uniqKey="Vecellio L">L. Vecellio</name></author><author><name sortKey="Diot, P" uniqKey="Diot P">P. Diot</name></author><author><name sortKey="Heuze Vourc H, N" uniqKey="Heuze Vourc H N">N. Heuze-Vourc'h</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Doremalen, N" uniqKey="Van Doremalen N">N. van Doremalen</name></author><author><name sortKey="Falzarano, D" uniqKey="Falzarano D">D. Falzarano</name></author><author><name sortKey="Ying, T" uniqKey="Ying T">T. Ying</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E. de Wit</name></author><author><name sortKey="Bushmaker, T" uniqKey="Bushmaker T">T. Bushmaker</name></author><author><name sortKey="Feldmann, F" uniqKey="Feldmann F">F. Feldmann</name></author><author><name sortKey="Okumura, A" uniqKey="Okumura A">A. Okumura</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Scott, D P" uniqKey="Scott D">D.P. Scott</name></author><author><name sortKey="Hanley, P W" uniqKey="Hanley P">P.W. Hanley</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name></author><author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name></author><author><name sortKey="Munster, V J" uniqKey="Munster V">V.J. Munster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Who" uniqKey="Who">WHO</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author><author><name sortKey="Perera, R A" uniqKey="Perera R">R.A. Perera</name></author><author><name sortKey="Kayali, G" uniqKey="Kayali G">G. Kayali</name></author><author><name sortKey="Meyerholz, D" uniqKey="Meyerholz D">D. Meyerholz</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author><author><name sortKey="Peiris, M" uniqKey="Peiris M">M. Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, Y" uniqKey="Zhao Y">Y. Zhao</name></author><author><name sortKey="Wang, C" uniqKey="Wang C">C. Wang</name></author><author><name sortKey="Qiu, B" uniqKey="Qiu B">B. Qiu</name></author><author><name sortKey="Li, C" uniqKey="Li C">C. Li</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author><author><name sortKey="Gai, W" uniqKey="Gai W">W. Gai</name></author><author><name sortKey="Zheng, X" uniqKey="Zheng X">X. Zheng</name></author><author><name sortKey="Wang, T" uniqKey="Wang T">T. Wang</name></author><author><name sortKey="Sun, W" uniqKey="Sun W">W. Sun</name></author><author><name sortKey="Yan, F" uniqKey="Yan F">F. Yan</name></author><author><name sortKey="Gao, Y" uniqKey="Gao Y">Y. Gao</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Yan, J" uniqKey="Yan J">J. Yan</name></author><author><name sortKey="Chen, L" uniqKey="Chen L">L. Chen</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author><author><name sortKey="Zhong, N" uniqKey="Zhong N">N. Zhong</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author><author><name sortKey="Yang, S" uniqKey="Yang S">S. Yang</name></author><author><name sortKey="Xia, X" uniqKey="Xia X">X. Xia</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Antiviral Res</journal-id><journal-id journal-id-type="iso-abbrev">Antiviral Res</journal-id><journal-title-group><journal-title>Antiviral Research</journal-title></journal-title-group><issn pub-type="ppub">0166-3542</issn><issn pub-type="epub">1872-9096</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">29885377</article-id><article-id pub-id-type="pmc">7113689</article-id><article-id pub-id-type="publisher-id">S0166-3542(18)30184-0</article-id><article-id pub-id-type="doi">10.1016/j.antiviral.2018.06.006</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Prophylactic and therapeutic efficacy of mAb treatment against MERS-CoV in common marmosets</article-title></title-group><contrib-group><contrib contrib-type="author" id="au1"><name><surname>de Wit</surname><given-names>Emmie</given-names></name><email>emmie.dewit@nih.gov</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">∗</xref></contrib><contrib contrib-type="author" id="au2"><name><surname>Feldmann</surname><given-names>Friederike</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au3"><name><surname>Okumura</surname><given-names>Atsushi</given-names></name><xref rid="aff3" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="au4"><name><surname>Horne</surname><given-names>Eva</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au5"><name><surname>Haddock</surname><given-names>Elaine</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au6"><name><surname>Saturday</surname><given-names>Greg</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au7"><name><surname>Scott</surname><given-names>Dana</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au8"><name><surname>Erlandson</surname><given-names>Karl J.</given-names></name><xref rid="aff4" ref-type="aff">d</xref></contrib><contrib contrib-type="author" id="au9"><name><surname>Stahl</surname><given-names>Neil</given-names></name><xref rid="aff5" ref-type="aff">e</xref></contrib><contrib contrib-type="author" id="au10"><name><surname>Lipsich</surname><given-names>Leah</given-names></name><xref rid="aff5" ref-type="aff">e</xref></contrib><contrib contrib-type="author" id="au11"><name><surname>Kyratsous</surname><given-names>Christos A.</given-names></name><xref rid="aff5" ref-type="aff">e</xref></contrib><contrib contrib-type="author" id="au12"><name><surname>Feldmann</surname><given-names>Heinz</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA</aff><aff id="aff2"><label>b</label>Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA</aff><aff id="aff3"><label>c</label>Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA</aff><aff id="aff4"><label>d</label>Biomedical Advanced Research and Development Authority, Washington, DC, USA</aff><aff id="aff5"><label>e</label>Regeneron Pharmaceuticals, Inc., Tarrytown, NY, USA</aff><author-notes><corresp id="cor1"><label>∗</label>Corresponding author. <email>emmie.dewit@nih.gov</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>7</day><month>6</month><year>2018</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>8</month><year>2018</year></pub-date><pub-date pub-type="epub"><day>7</day><month>6</month><year>2018</year></pub-date><volume>156</volume><fpage>64</fpage><lpage>71</lpage><history><date date-type="received"><day>2</day><month>4</month><year>2018</year></date><date date-type="rev-recd"><day>4</day><month>6</month><year>2018</year></date><date date-type="accepted"><day>5</day><month>6</month><year>2018</year></date></history><permissions><copyright-statement>© 2018 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2018</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract id="abs0010"><p>The high case-fatality rate of confirmed MERS-CoV infections underlines the urgent need for an effective treatment to reduce the disease severity and mortality. REGN3051 and REGN3048 are two fully human neutralizing monoclonal antibodies (mAb) against MERS-CoV that reduced virus replication in mice expressing human DPP4 upon prophylactic and therapeutic treatment. Here, we evaluated the prophylactic and therapeutic efficacy of REGN3048 and REGN3051 in the common marmoset model of MERS-CoV infection. Intravenous administration of mAb resulted in high levels of MERS-CoV-neutralizing activity in circulating blood. When animals were treated with mAbs one day before challenge, respiratory disease was less severe and, in animals treated with both REGN3048 and REGN3051, viral loads in the lungs were reduced. However, therapeutic treatment on day one after challenge was less efficacious as it did not prevent the development of severe respiratory disease and all treated animals developed bronchointerstitial pneumonia of similar severity as the control animals. Thus, mAb administration may be more effective in a prophylactic treatment regimen rather than treatment of MERS.</p></abstract><abstract abstract-type="author-highlights" id="abs0015"><title>Highlights</title><p><list list-type="simple" id="ulist0010"><list-item id="u0010"><label>•</label><p id="p0010">REGN3051 and REGN3048 are two human MERS-CoV-neutralizing monoclonal antibodies that reduced virus replication in mice.</p></list-item><list-item id="u0015"><label>•</label><p id="p0015">We evaluated the prophylactic and therapeutic efficacy of these mAbs in the common marmoset model of MERS-CoV infection.</p></list-item><list-item id="u0020"><label>•</label><p id="p0020">Prophylactic treatment with REGN3048 and REGN3051 resulted in less severe respiratory disease and reduced viral lung loads.</p></list-item><list-item id="u0025"><label>•</label><p id="p0025">Therapeutic treatment was less efficacious as it did not prevent the development of severe respiratory disease.</p></list-item></list></p></abstract><kwd-group id="kwrds0010"><title>Keywords</title><kwd>MERS-CoV</kwd><kwd>Monoclonal antibody treatment</kwd><kwd>Neutralizing antibody</kwd><kwd>Animal model</kwd><kwd>Common marmoset</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country><region><li>District de Columbia</li><li>Montana</li><li>État de New York</li></region><settlement><li>New York</li></settlement><orgName><li>Université Columbia</li></orgName></list><tree><country name="États-Unis"><region name="Montana"><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name></region><name sortKey="Erlandson, Karl J" sort="Erlandson, Karl J" uniqKey="Erlandson K" first="Karl J." last="Erlandson">Karl J. Erlandson</name><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name><name sortKey="Horne, Eva" sort="Horne, Eva" uniqKey="Horne E" first="Eva" last="Horne">Eva Horne</name><name sortKey="Kyratsous, Christos A" sort="Kyratsous, Christos A" uniqKey="Kyratsous C" first="Christos A." last="Kyratsous">Christos A. Kyratsous</name><name sortKey="Lipsich, Leah" sort="Lipsich, Leah" uniqKey="Lipsich L" first="Leah" last="Lipsich">Leah Lipsich</name><name sortKey="Okumura, Atsushi" sort="Okumura, Atsushi" uniqKey="Okumura A" first="Atsushi" last="Okumura">Atsushi Okumura</name><name sortKey="Saturday, Greg" sort="Saturday, Greg" uniqKey="Saturday G" first="Greg" last="Saturday">Greg Saturday</name><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name><name sortKey="Stahl, Neil" sort="Stahl, Neil" uniqKey="Stahl N" first="Neil" last="Stahl">Neil Stahl</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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