Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay
Identifieur interne : 000314 ( Pmc/Checkpoint ); précédent : 000313; suivant : 000315Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay
Auteurs :Source :
- MERS Coronavirus ; 2019.
Abstract
The microneutralization (MN) assay is a standard and important technique in virology, immunology, and epidemiology. It is a highly specific and sensitive assay for evaluating virus-specific neutralizing antibodies (nAbs) in human and animal sera. It provides the most precise answer to whether or not an individual or animal has antibodies that can neutralize or inhibit the infectivity of a specific virus strain. However, using live virus-based MN assay might require working under high containment facilities especially when dealing with high-risk pathogens such as the Middle East respiratory syndrome-coronavirus (MERS-CoV). In this chapter, we describe the isolation, amplification, and titration of MERS-CoV, as well as detailed MN assay to measure nAb levels in sera from different mammalian species.
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DOI: 10.1007/978-1-0716-0211-9_9
PubMed: 31883091
PubMed Central: 7121888
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay</title></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31883091</idno><idno type="pmc">7121888</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121888</idno><idno type="RBID">PMC:7121888</idno><idno type="doi">10.1007/978-1-0716-0211-9_9</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000183</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000183</idno><idno type="wicri:Area/Pmc/Curation">000183</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000183</idno><idno type="wicri:Area/Pmc/Checkpoint">000314</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000314</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay</title></analytic><series><title level="j">MERS Coronavirus</title><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">The microneutralization (MN) assay is a standard and important technique in virology, immunology, and epidemiology. It is a highly specific and sensitive assay for evaluating virus-specific neutralizing antibodies (nAbs) in human and animal sera. It provides the most precise answer to whether or not an individual or animal has antibodies that can neutralize or inhibit the infectivity of a specific virus strain. However, using live virus-based MN assay might require working under high containment facilities especially when dealing with high-risk pathogens such as the Middle East respiratory syndrome-coronavirus (MERS-CoV). In this chapter, we describe the isolation, amplification, and titration of MERS-CoV, as well as detailed MN assay to measure nAb levels in sera from different mammalian species.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alagaili, An" uniqKey="Alagaili A">AN Alagaili</name></author><author><name sortKey="Briese, T" uniqKey="Briese T">T Briese</name></author><author><name sortKey="Mishra, N" uniqKey="Mishra N">N Mishra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Azhar, Ei" uniqKey="Azhar E">EI Azhar</name></author><author><name sortKey="El Kafrawy, Sa" uniqKey="El Kafrawy S">SA 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Omrani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author><author><name sortKey="Alshukairi, An" uniqKey="Alshukairi A">AN Alshukairi</name></author><author><name sortKey="Baharoon, Sa" uniqKey="Baharoon S">SA Baharoon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Al Amri, Ss" uniqKey="Al Amri S">SS Al-Amri</name></author><author><name sortKey="Abbas, At" uniqKey="Abbas A">AT Abbas</name></author><author><name sortKey="Siddiq, La" uniqKey="Siddiq L">LA Siddiq</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perera, Ra" uniqKey="Perera R">RA Perera</name></author><author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name></author><author><name sortKey="Gomaa, Mr" uniqKey="Gomaa M">MR Gomaa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, C" uniqKey="Reusken C">C Reusken</name></author><author><name sortKey="Mou, H" uniqKey="Mou H">H Mou</name></author><author><name sortKey="Godeke, Gj" uniqKey="Godeke G">GJ Godeke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Al Abdallat, Mm" uniqKey="Al Abdallat M">MM Al-Abdallat</name></author><author><name sortKey="Payne, Dc" uniqKey="Payne D">DC Payne</name></author><author><name sortKey="Alqasrawi, S" uniqKey="Alqasrawi S">S Alqasrawi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hemida, Mg" uniqKey="Hemida M">MG Hemida</name></author><author><name sortKey="Perera, Ra" uniqKey="Perera R">RA Perera</name></author><author><name sortKey="Al Jassim, Ra" uniqKey="Al Jassim R">RA Al Jassim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kleine Weber, H" uniqKey="Kleine Weber H">H Kleine-Weber</name></author><author><name sortKey="Elzayat, Mt" uniqKey="Elzayat M">MT Elzayat</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Park, Sw" uniqKey="Park S">SW Park</name></author><author><name sortKey="Perera, Ra" uniqKey="Perera R">RA Perera</name></author><author><name sortKey="Choe, Pg" uniqKey="Choe P">PG Choe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fukushi, S" uniqKey="Fukushi S">S Fukushi</name></author><author><name sortKey="Fukuma, A" uniqKey="Fukuma A">A Fukuma</name></author><author><name sortKey="Kurosu, T" uniqKey="Kurosu T">T Kurosu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Trivedi, S" uniqKey="Trivedi S">S Trivedi</name></author><author><name sortKey="Miao, C" uniqKey="Miao C">C Miao</name></author><author><name sortKey="Al Abdallat, Mm" uniqKey="Al Abdallat M">MM Al-Abdallat</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hashem, Am" uniqKey="Hashem A">AM Hashem</name></author><author><name sortKey="Al Amri, Ss" uniqKey="Al Amri S">SS Al-Amri</name></author><author><name sortKey="Al Subhi, Tl" uniqKey="Al Subhi T">TL Al-Subhi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reed, Lj" uniqKey="Reed L">LJ Reed</name></author><author><name sortKey="Muench, H" uniqKey="Muench H">H Muench</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="chapter-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="publisher-id">978-1-0716-0211-9</journal-id><journal-id journal-id-type="doi">10.1007/978-1-0716-0211-9</journal-id><journal-id journal-id-type="nlm-ta">MERS Coronavirus</journal-id><journal-title-group><journal-title>MERS Coronavirus</journal-title><journal-subtitle>Methods and Protocols </journal-subtitle></journal-title-group><isbn publication-format="print">978-1-0716-0210-2</isbn><isbn publication-format="electronic">978-1-0716-0211-9</isbn></journal-meta><article-meta><article-id pub-id-type="pmid">31883091</article-id><article-id pub-id-type="pmc">7121888</article-id><article-id pub-id-type="publisher-id">9</article-id><article-id pub-id-type="doi">10.1007/978-1-0716-0211-9_9</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay</article-title></title-group><contrib-group content-type="book editors"><contrib contrib-type="editor"><name><surname>Vijay</surname><given-names>Rahul</given-names></name><address><email>rahul-vijay@uiowa.edu</email></address><xref ref-type="aff" rid="Aff2"></xref></contrib><aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.214572.7</institution-id><institution-id institution-id-type="ISNI">0000 0004 1936 8294</institution-id><institution>Department of Microbiology and Immunology,</institution><institution>University of Iowa,</institution></institution-wrap>Iowa City, IA USA</aff></contrib-group><contrib-group><contrib contrib-type="author"><name><surname>Algaissi</surname><given-names>Abdullah</given-names></name><xref ref-type="aff" rid="Aff3">3</xref><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Hashem</surname><given-names>Anwar M.</given-names></name><address><email>amhashem@kau.edu.sa</email></address><xref ref-type="aff" rid="Aff5">5</xref><xref ref-type="aff" rid="Aff6">6</xref></contrib><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="GRID">grid.176731.5</institution-id><institution-id institution-id-type="ISNI">0000 0001 1547 9964</institution-id><institution>Departments of Microbiology and Immunology,</institution><institution>University of Texas Medical Branch,</institution></institution-wrap>Galveston, TX USA</aff><aff id="Aff4"><label>4</label><institution-wrap><institution-id institution-id-type="GRID">grid.411831.e</institution-id><institution-id institution-id-type="ISNI">0000 0004 0398 1027</institution-id><institution>Department of Medical Laboratories Technology,</institution><institution>College of Applied Medical Sciences, Jazan University,</institution></institution-wrap>Jazan, Saudi Arabia</aff><aff id="Aff5"><label>5</label><institution-wrap><institution-id institution-id-type="GRID">grid.412125.1</institution-id><institution-id institution-id-type="ISNI">0000 0001 0619 1117</institution-id><institution>Department of Medical Microbiology and Parasitology, Faculty of Medicine,</institution><institution>King Abdulaziz University,</institution></institution-wrap>Jeddah, Saudi Arabia</aff><aff id="Aff6"><label>6</label><institution-wrap><institution-id institution-id-type="GRID">grid.412125.1</institution-id><institution-id institution-id-type="ISNI">0000 0001 0619 1117</institution-id><institution>Vaccines and Immunotherapy Unit, King Fahd Medical Research Center,</institution><institution>King Abdulaziz University,</institution></institution-wrap>Jeddah, Saudi Arabia</aff></contrib-group><pub-date pub-type="epub"><day>14</day><month>9</month><year>2019</year></pub-date><pub-date pub-type="collection"><year>2020</year></pub-date><volume>2099</volume><fpage>107</fpage><lpage>116</lpage><permissions><copyright-statement>© Springer Science+Business Media, LLC, part of Springer Nature 2020</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p id="Par1">The microneutralization (MN) assay is a standard and important technique in virology, immunology, and epidemiology. It is a highly specific and sensitive assay for evaluating virus-specific neutralizing antibodies (nAbs) in human and animal sera. It provides the most precise answer to whether or not an individual or animal has antibodies that can neutralize or inhibit the infectivity of a specific virus strain. However, using live virus-based MN assay might require working under high containment facilities especially when dealing with high-risk pathogens such as the Middle East respiratory syndrome-coronavirus (MERS-CoV). In this chapter, we describe the isolation, amplification, and titration of MERS-CoV, as well as detailed MN assay to measure nAb levels in sera from different mammalian species.</p></abstract><kwd-group xml:lang="en"><title>Key words</title><kwd>MERS-CoV</kwd><kwd>Neutralizing antibodies</kwd><kwd>Microneutralization</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer Science+Business Media, LLC, part of Springer Nature 2020</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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