Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells
Identifieur interne : 000210 ( Pmc/Checkpoint ); précédent : 000209; suivant : 000211Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells
Auteurs : Tatsuya Kato [Japon] ; Yoshihiro Takami [Japon] ; Vipin Kumar Deo [Japon] ; Enoch Y. Park [Japon]Source :
- Journal of Biotechnology [ 0168-1656 ] ; 2019.
Abstract
Virus-like particle mimetic nanovesicles were prepared using insect cells. Surfactant treatment or mechanical extrusion make it possible to display the S protein on VLPs. Mechanical extrusion is more effective way to display the S protein on VLPs. S protein on the surface of nanovesicles were confirmed by immuno-TEM. The purified S protein (SοTM) has the ability to bind a receptor of MERS-CoV.
Url:
DOI: 10.1016/j.jbiotec.2019.10.007
PubMed: 31614169
PubMed Central: 7114102
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells</title><author><name sortKey="Kato, Tatsuya" sort="Kato, Tatsuya" uniqKey="Kato T" first="Tatsuya" last="Kato">Tatsuya Kato</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Takami, Yoshihiro" sort="Takami, Yoshihiro" uniqKey="Takami Y" first="Yoshihiro" last="Takami">Yoshihiro Takami</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Kumar Deo, Vipin" sort="Kumar Deo, Vipin" uniqKey="Kumar Deo V" first="Vipin" last="Kumar Deo">Vipin Kumar Deo</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Park, Enoch Y" sort="Park, Enoch Y" uniqKey="Park E" first="Enoch Y." last="Park">Enoch Y. Park</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31614169</idno><idno type="pmc">7114102</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114102</idno><idno type="RBID">PMC:7114102</idno><idno type="doi">10.1016/j.jbiotec.2019.10.007</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000D87</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D87</idno><idno type="wicri:Area/Pmc/Curation">000D87</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D87</idno><idno type="wicri:Area/Pmc/Checkpoint">000210</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000210</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells</title><author><name sortKey="Kato, Tatsuya" sort="Kato, Tatsuya" uniqKey="Kato T" first="Tatsuya" last="Kato">Tatsuya Kato</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Takami, Yoshihiro" sort="Takami, Yoshihiro" uniqKey="Takami Y" first="Yoshihiro" last="Takami">Yoshihiro Takami</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Kumar Deo, Vipin" sort="Kumar Deo, Vipin" uniqKey="Kumar Deo V" first="Vipin" last="Kumar Deo">Vipin Kumar Deo</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Park, Enoch Y" sort="Park, Enoch Y" uniqKey="Park E" first="Enoch Y." last="Park">Enoch Y. Park</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Biotechnology</title><idno type="ISSN">0168-1656</idno><idno type="eISSN">1873-4863</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Virus-like particle mimetic nanovesicles were prepared using insect cells.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Surfactant treatment or mechanical extrusion make it possible to display the S protein on VLPs.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">Mechanical extrusion is more effective way to display the S protein on VLPs.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">S protein on the surface of nanovesicles were confirmed by immuno-TEM.</p></list-item><list-item id="lsti0025"><label>•</label><p id="par0025">The purified S protein (SοTM) has the ability to bind a receptor of MERS-CoV.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, J F" uniqKey="Chan J">J.F. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Biotechnol</journal-id><journal-id journal-id-type="iso-abbrev">J. Biotechnol</journal-id><journal-title-group><journal-title>Journal of Biotechnology</journal-title></journal-title-group><issn pub-type="ppub">0168-1656</issn><issn pub-type="epub">1873-4863</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31614169</article-id><article-id pub-id-type="pmc">7114102</article-id><article-id pub-id-type="publisher-id">S0168-1656(19)30887-9</article-id><article-id pub-id-type="doi">10.1016/j.jbiotec.2019.10.007</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Kato</surname><given-names>Tatsuya</given-names></name><xref rid="aff0005" ref-type="aff">a</xref><xref rid="aff0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Takami</surname><given-names>Yoshihiro</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Kumar Deo</surname><given-names>Vipin</given-names></name><xref rid="aff0015" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Park</surname><given-names>Enoch Y.</given-names></name><email>park.enoch@shizuoka.ac.jp</email><xref rid="aff0005" ref-type="aff">a</xref><xref rid="aff0010" ref-type="aff">b</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff0005"><label>a</label>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</aff><aff id="aff0010"><label>b</label>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</aff><aff id="aff0015"><label>c</label>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author at: Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan. <email>park.enoch@shizuoka.ac.jp</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>12</day><month>10</month><year>2019</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>20</day><month>12</month><year>2019</year></pub-date><pub-date pub-type="epub"><day>12</day><month>10</month><year>2019</year></pub-date><volume>306</volume><fpage>177</fpage><lpage>184</lpage><history><date date-type="received"><day>12</day><month>6</month><year>2019</year></date><date date-type="rev-recd"><day>26</day><month>9</month><year>2019</year></date><date date-type="accepted"><day>7</day><month>10</month><year>2019</year></date></history><permissions><copyright-statement>© 2019 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2019</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract abstract-type="author-highlights" id="abs0005"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Virus-like particle mimetic nanovesicles were prepared using insect cells.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Surfactant treatment or mechanical extrusion make it possible to display the S protein on VLPs.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">Mechanical extrusion is more effective way to display the S protein on VLPs.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">S protein on the surface of nanovesicles were confirmed by immuno-TEM.</p></list-item><list-item id="lsti0025"><label>•</label><p id="par0025">The purified S protein (SοTM) has the ability to bind a receptor of MERS-CoV.</p></list-item></list></p></abstract><abstract id="abs0010"><p>Middle East respiratory syndrome coronavirus (MERS-CoV) first emerged in 2012, and over 2000 infections and 800 deaths have been confirmed in 27 countries. However, to date, no commercial vaccine is available. In this study, structural proteins of MERS-CoV were expressed in silkworm larvae and Bm5 cells for the development of vaccine candidates against MERS-CoV and diagnostic methods. The spike (S) protein of MERS-CoV lacking its transmembrane and cytoplasmic domains (SΔTM) was secreted into the hemolymph of silkworm larvae using a bombyxin signal peptide and purified using affinity chromatography. The purified SΔTM forms small nanoparticles as well as the full-length S protein and has the ability to bind human dipeptidyl peptidase 4 (DPP4), which is a receptor of MERS-CoV. These results indicate that bioactive SΔTM was expressed in silkworm larvae. To produce MERS-CoV-like particles (MERS-CoV-LPs), the coexpression of spike proteins was performed in Bm5 cells and envelope (E) and membrane (M) proteins secreted E and M proteins extracellularly, suggesting that MERS-CoV-LPs may be formed. However, this S protein was not displayed on virus-like particles (VLPs) even though E and M proteins were secreted into the culture supernatant. By surfactant treatment and mechanical extrusion using S protein- or three structural protein-expressing Bm5 cells, S protein-displaying nanovesicles with diameters of approximately 100-200 nm were prepared and confirmed by immuno-TEM. The mechanical extrusion method is favorable for obtaining uniform recombinant protein-displaying nanovesicles from cultured cells. The purified SΔTM from silkworm larvae and S protein-displaying nanovesicles from Bm5 cells may lead to the development of nanoparticle-based vaccines against MERS-CoV and the diagnostic detection of MERS-CoV.</p></abstract><kwd-group id="kwd0005"><title>Keywords</title><kwd>Virus-like particle</kwd><kwd>Middle East respiratory syndrome</kwd><kwd>Coronavirus</kwd><kwd>Silkworm</kwd><kwd>Vaccine</kwd><kwd>Bm5 cell</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Kato, Tatsuya" sort="Kato, Tatsuya" uniqKey="Kato T" first="Tatsuya" last="Kato">Tatsuya Kato</name></noRegion><name sortKey="Kato, Tatsuya" sort="Kato, Tatsuya" uniqKey="Kato T" first="Tatsuya" last="Kato">Tatsuya Kato</name><name sortKey="Kumar Deo, Vipin" sort="Kumar Deo, Vipin" uniqKey="Kumar Deo V" first="Vipin" last="Kumar Deo">Vipin Kumar Deo</name><name sortKey="Park, Enoch Y" sort="Park, Enoch Y" uniqKey="Park E" first="Enoch Y." last="Park">Enoch Y. Park</name><name sortKey="Park, Enoch Y" sort="Park, Enoch Y" uniqKey="Park E" first="Enoch Y." last="Park">Enoch Y. Park</name><name sortKey="Takami, Yoshihiro" sort="Takami, Yoshihiro" uniqKey="Takami Y" first="Yoshihiro" last="Takami">Yoshihiro Takami</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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