Identification of Coronavirus Isolated from a Patient in Korea with COVID-19
Identifieur interne : 000075 ( Pmc/Checkpoint ); précédent : 000074; suivant : 000076Identification of Coronavirus Isolated from a Patient in Korea with COVID-19
Auteurs : Jeong-Min Kim [Corée du Sud] ; Yoon-Seok Chung [Corée du Sud] ; Hye Jun Jo [Corée du Sud] ; Nam-Joo Lee [Corée du Sud] ; Mi Seon Kim [Corée du Sud] ; Sang Hee Woo [Corée du Sud] ; Sehee Park [Corée du Sud] ; Jee Woong Kim [Corée du Sud] ; Heui Man Kim [Corée du Sud] ; Myung-Guk Han [Corée du Sud]Source :
- Osong Public Health and Research Perspectives [ 2210-9099 ] ; 2020.
Abstract
Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.
Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.
The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.
SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
Url:
DOI: 10.24171/j.phrp.2020.11.1.02
PubMed: 32149036
PubMed Central: 7045880
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:7045880Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of Coronavirus Isolated from a Patient in Korea with COVID-19</title><author><name sortKey="Kim, Jeong Min" sort="Kim, Jeong Min" uniqKey="Kim J" first="Jeong-Min" last="Kim">Jeong-Min Kim</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Chung, Yoon Seok" sort="Chung, Yoon Seok" uniqKey="Chung Y" first="Yoon-Seok" last="Chung">Yoon-Seok Chung</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Jo, Hye Jun" sort="Jo, Hye Jun" uniqKey="Jo H" first="Hye Jun" last="Jo">Hye Jun Jo</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Nam Joo" sort="Lee, Nam Joo" uniqKey="Lee N" first="Nam-Joo" last="Lee">Nam-Joo Lee</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Mi Seon" sort="Kim, Mi Seon" uniqKey="Kim M" first="Mi Seon" last="Kim">Mi Seon Kim</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Woo, Sang Hee" sort="Woo, Sang Hee" uniqKey="Woo S" first="Sang Hee" last="Woo">Sang Hee Woo</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Park, Sehee" sort="Park, Sehee" uniqKey="Park S" first="Sehee" last="Park">Sehee Park</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Jee Woong" sort="Kim, Jee Woong" uniqKey="Kim J" first="Jee Woong" last="Kim">Jee Woong Kim</name><affiliation wicri:level="1"><nlm:aff id="af2-ophrp-11-3">Division of Biosafety Evaluation and Control, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biosafety Evaluation and Control, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Heui Man" sort="Kim, Heui Man" uniqKey="Kim H" first="Heui Man" last="Kim">Heui Man Kim</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Han, Myung Guk" sort="Han, Myung Guk" uniqKey="Han M" first="Myung-Guk" last="Han">Myung-Guk Han</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32149036</idno><idno type="pmc">7045880</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045880</idno><idno type="RBID">PMC:7045880</idno><idno type="doi">10.24171/j.phrp.2020.11.1.02</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">000F77</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F77</idno><idno type="wicri:Area/Pmc/Curation">000F77</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000F77</idno><idno type="wicri:Area/Pmc/Checkpoint">000075</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000075</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of Coronavirus Isolated from a Patient in Korea with COVID-19</title><author><name sortKey="Kim, Jeong Min" sort="Kim, Jeong Min" uniqKey="Kim J" first="Jeong-Min" last="Kim">Jeong-Min Kim</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Chung, Yoon Seok" sort="Chung, Yoon Seok" uniqKey="Chung Y" first="Yoon-Seok" last="Chung">Yoon-Seok Chung</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Jo, Hye Jun" sort="Jo, Hye Jun" uniqKey="Jo H" first="Hye Jun" last="Jo">Hye Jun Jo</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Nam Joo" sort="Lee, Nam Joo" uniqKey="Lee N" first="Nam-Joo" last="Lee">Nam-Joo Lee</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Mi Seon" sort="Kim, Mi Seon" uniqKey="Kim M" first="Mi Seon" last="Kim">Mi Seon Kim</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Woo, Sang Hee" sort="Woo, Sang Hee" uniqKey="Woo S" first="Sang Hee" last="Woo">Sang Hee Woo</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Park, Sehee" sort="Park, Sehee" uniqKey="Park S" first="Sehee" last="Park">Sehee Park</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Jee Woong" sort="Kim, Jee Woong" uniqKey="Kim J" first="Jee Woong" last="Kim">Jee Woong Kim</name><affiliation wicri:level="1"><nlm:aff id="af2-ophrp-11-3">Division of Biosafety Evaluation and Control, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biosafety Evaluation and Control, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Heui Man" sort="Kim, Heui Man" uniqKey="Kim H" first="Heui Man" last="Kim">Heui Man Kim</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Han, Myung Guk" sort="Han, Myung Guk" uniqKey="Han M" first="Myung-Guk" last="Han">Myung-Guk Han</name><affiliation wicri:level="1"><nlm:aff id="af1-ophrp-11-3">Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju</wicri:regionArea><wicri:noRegion>Cheongju</wicri:noRegion></affiliation></author></analytic><series><title level="j">Osong Public Health and Research Perspectives</title><idno type="ISSN">2210-9099</idno><idno type="eISSN">2233-6052</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Objectives</title><p>Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.</p></sec><sec><title>Methods</title><p>Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.</p></sec><sec><title>Results</title><p>The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.</p></sec><sec><title>Conclusion</title><p>SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cui, J" uniqKey="Cui J">J Cui</name></author><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monchatre Leroy, E" uniqKey="Monchatre Leroy E">E Monchatre-Leroy</name></author><author><name sortKey="Boue, F" uniqKey="Boue F">F Boue</name></author><author><name sortKey="Boucher, Jm" uniqKey="Boucher J">JM Boucher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cong, Y" uniqKey="Cong Y">Y Cong</name></author><author><name sortKey="Ren, X" uniqKey="Ren X">X Ren</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tortorici, Ma" uniqKey="Tortorici M">MA Tortorici</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D Veesler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Muth, D" uniqKey="Muth D">D Muth</name></author><author><name sortKey="Niemeyer, D" uniqKey="Niemeyer D">D Niemeyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Wit, E" uniqKey="De Wit E">E De Wit</name></author><author><name sortKey="Van Doremalen, N" uniqKey="Van Doremalen N">N Van Doremalen</name></author><author><name sortKey="Falzarano, D" uniqKey="Falzarano D">D Falzarano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paraskevis, D" uniqKey="Paraskevis D">D Paraskevis</name></author><author><name sortKey="Kostaki, Eg" uniqKey="Kostaki E">EG Kostaki</name></author><author><name sortKey="Magiorkinis, G" uniqKey="Magiorkinis G">G Magiorkinis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hui, Ds" uniqKey="Hui D">DS Hui</name></author><author><name sortKey="Azhar, Ie" uniqKey="Azhar I">IE Azhar</name></author><author><name sortKey="Madani, Ta" uniqKey="Madani T">TA Madani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rubin, Ej" uniqKey="Rubin E">EJ Rubin</name></author><author><name sortKey="Baden, Lr" uniqKey="Baden L">LR Baden</name></author><author><name sortKey="Morrissey, S" uniqKey="Morrissey S">S Morrissey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Favre, G" uniqKey="Favre G">G Favre</name></author><author><name sortKey="Pomar, L" uniqKey="Pomar L">L Pomar</name></author><author><name sortKey="Musso, D" uniqKey="Musso D">D Musso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, Y" uniqKey="Pan Y">Y Pan</name></author><author><name sortKey="Guan, H" uniqKey="Guan H">H Guan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O Landt</name></author><author><name sortKey="Kaiser, M" uniqKey="Kaiser M">M Kaiser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Wang, Xg" uniqKey="Wang X">XG Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hua, Y" uniqKey="Hua Y">Y Hua</name></author><author><name sortKey="Laserstein, P" uniqKey="Laserstein P">P Laserstein</name></author><author><name sortKey="Helmstaedter, M" uniqKey="Helmstaedter M">M Helmstaedter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jan, Mo" uniqKey="Jan M">MO Jan</name></author><author><name sortKey="Bridget, B" uniqKey="Bridget B">B Bridget</name></author><author><name sortKey="Susan, C" uniqKey="Susan C">C Susan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, A" uniqKey="Zhu A">A Zhu</name></author><author><name sortKey="Zhang, Dz" uniqKey="Zhang D">DZ Zhang</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W Wang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Osong Public Health Res Perspect</journal-id><journal-id journal-id-type="iso-abbrev">Osong Public Health Res Perspect</journal-id><journal-id journal-id-type="publisher-id">kphrp1</journal-id><journal-title-group><journal-title>Osong Public Health and Research Perspectives</journal-title></journal-title-group><issn pub-type="ppub">2210-9099</issn><issn pub-type="epub">2233-6052</issn><publisher><publisher-name>Korea Centers for Disease Control and Prevention</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32149036</article-id><article-id pub-id-type="pmc">7045880</article-id><article-id pub-id-type="doi">10.24171/j.phrp.2020.11.1.02</article-id><article-id pub-id-type="publisher-id">ophrp-11-3</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Identification of Coronavirus Isolated from a Patient in Korea with COVID-19</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Jeong-Min</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8240-0395</contrib-id><xref ref-type="aff" rid="af1-ophrp-11-3">a</xref></contrib><contrib contrib-type="author"><name><surname>Chung</surname><given-names>Yoon-Seok</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4562-5533</contrib-id><xref ref-type="aff" rid="af1-ophrp-11-3">a</xref></contrib><contrib contrib-type="author"><name><surname>Jo</surname><given-names>Hye Jun</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2416-1119</contrib-id><xref ref-type="aff" rid="af1-ophrp-11-3">a</xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>Nam-Joo</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9488-9845</contrib-id><xref ref-type="aff" rid="af1-ophrp-11-3">a</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Mi Seon</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8503-9112</contrib-id><xref ref-type="aff" rid="af1-ophrp-11-3">a</xref></contrib><contrib contrib-type="author"><name><surname>Woo</surname><given-names>Sang Hee</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9666-3671</contrib-id><xref ref-type="aff" rid="af1-ophrp-11-3">a</xref></contrib><contrib contrib-type="author"><name><surname>Park</surname><given-names>Sehee</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4799-2241</contrib-id><xref ref-type="aff" rid="af1-ophrp-11-3">a</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Jee Woong</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4164-1886</contrib-id><xref ref-type="aff" rid="af2-ophrp-11-3">b</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Heui Man</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2908-6437</contrib-id><xref ref-type="aff" rid="af1-ophrp-11-3">a</xref></contrib><contrib contrib-type="author"><name><surname>Han</surname><given-names>Myung-Guk</given-names></name><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3543-1826</contrib-id><xref ref-type="aff" rid="af1-ophrp-11-3">a</xref><xref rid="c1-ophrp-11-3" ref-type="corresp">*</xref></contrib></contrib-group><aff id="af1-ophrp-11-3"><label>a</label>Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea</aff><aff id="af2-ophrp-11-3"><label>b</label>Division of Biosafety Evaluation and Control, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju, Korea</aff><author-notes><corresp id="c1-ophrp-11-3"><label>*</label>Corresponding author: Myung-Guk Han, Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea, E-mail: <email>mghan@korea.kr</email></corresp></author-notes><pub-date pub-type="ppub"><month>2</month><year>2020</year></pub-date><volume>11</volume><issue>1</issue><fpage>3</fpage><lpage>7</lpage><history><date date-type="received"><day>13</day><month>2</month><year>2020</year></date><date date-type="rev-recd"><day>18</day><month>2</month><year>2020</year></date><date date-type="accepted"><day>19</day><month>2</month><year>2020</year></date></history><permissions><copyright-statement>Copyright ©2020, Korea Centers for Disease Control and Prevention</copyright-statement><copyright-year>2020</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-nd/4.0/"><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>This is an open access article under the CC BY-NC-ND license (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-nd/4.0/">http://creativecommons.org/licenses/by-nc-nd/4.0/</ext-link>).</license-p></license></permissions><abstract><sec><title>Objectives</title><p>Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.</p></sec><sec><title>Methods</title><p>Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.</p></sec><sec><title>Results</title><p>The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.</p></sec><sec><title>Conclusion</title><p>SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.</p></sec></abstract><kwd-group><kwd>COVID-19</kwd><kwd>coronavirus</kwd><kwd>SARS-CoV-2</kwd><kwd>isolation</kwd><kwd>pneumonia</kwd></kwd-group></article-meta></front><floats-group><fig id="f1-ophrp-11-3" orientation="portrait" position="float"><label>Figure 1</label><caption><p>Cytopathic effect of SARS-CoV-2 on Vero cells. (A) Mock inoculated cells (B) SARS-CoV-2 inoculated cells.</p></caption><graphic xlink:href="ophrp-11-3f1"></graphic></fig><fig id="f2-ophrp-11-3" orientation="portrait" position="float"><label>Figure 2</label><caption><p>Thin section electron micrographs of Vero cells infected with SARS-CoV-2. Electron micrographs show representative thin sections of Vero cells infected with SARS-CoV-2; cells were collected at 48 hours after infection for examination by electron microscopy. White arrows point to aggregates of assembled intracellular virions.</p></caption><graphic xlink:href="ophrp-11-3f2"></graphic></fig><fig id="f3-ophrp-11-3" orientation="portrait" position="float"><label>Figure 3</label><caption><p>Phylogenetic tree analysis of SARS-CoV-2 based on full genome nucleotide sequences using the neighbor-joining tree. Values on branches are shown as percentages based on 1,000 bootstrap replicates. (A) Gene analysis of SARS-CoV-2 and other coronaviruses, (B) gene analysis of SARS-CoV-2 and BetaCoV/Korea/KCDC03/2020.</p></caption><graphic xlink:href="ophrp-11-3f3"></graphic></fig></floats-group></pmc><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Kim, Jeong Min" sort="Kim, Jeong Min" uniqKey="Kim J" first="Jeong-Min" last="Kim">Jeong-Min Kim</name></noRegion><name sortKey="Chung, Yoon Seok" sort="Chung, Yoon Seok" uniqKey="Chung Y" first="Yoon-Seok" last="Chung">Yoon-Seok Chung</name><name sortKey="Han, Myung Guk" sort="Han, Myung Guk" uniqKey="Han M" first="Myung-Guk" last="Han">Myung-Guk Han</name><name sortKey="Jo, Hye Jun" sort="Jo, Hye Jun" uniqKey="Jo H" first="Hye Jun" last="Jo">Hye Jun Jo</name><name sortKey="Kim, Heui Man" sort="Kim, Heui Man" uniqKey="Kim H" first="Heui Man" last="Kim">Heui Man Kim</name><name sortKey="Kim, Jee Woong" sort="Kim, Jee Woong" uniqKey="Kim J" first="Jee Woong" last="Kim">Jee Woong Kim</name><name sortKey="Kim, Mi Seon" sort="Kim, Mi Seon" uniqKey="Kim M" first="Mi Seon" last="Kim">Mi Seon Kim</name><name sortKey="Lee, Nam Joo" sort="Lee, Nam Joo" uniqKey="Lee N" first="Nam-Joo" last="Lee">Nam-Joo Lee</name><name sortKey="Park, Sehee" sort="Park, Sehee" uniqKey="Park S" first="Sehee" last="Park">Sehee Park</name><name sortKey="Woo, Sang Hee" sort="Woo, Sang Hee" uniqKey="Woo S" first="Sang Hee" last="Woo">Sang Hee Woo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000075 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000075 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:7045880
|texte= Identification of Coronavirus Isolated from a Patient in Korea with COVID-19
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:32149036" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |