Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells
Identifieur interne : 002366 ( Ncbi/Merge ); précédent : 002365; suivant : 002367Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells
Auteurs : Tatsuya Kato [Japon] ; Yoshihiro Takami [Japon] ; Vipin Kumar Deo [Japon] ; Enoch Y. Park [Japon]Source :
- Journal of Biotechnology [ 0168-1656 ] ; 2019.
Descripteurs français
- KwdFr :
- Animaux, Bombyx (génétique), Bombyx (métabolisme), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Dipeptidyl peptidase 4 (métabolisme), Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (isolement et purification), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Hémolymphe (métabolisme), Infections à coronavirus (), Infections à coronavirus (diagnostic), Larve (métabolisme), Lignée cellulaire, Matériaux biomimétiques (), Matériaux biomimétiques (métabolisme), Protéines d'insecte (génétique), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (isolement et purification), Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (génétique), Protéines de la matrice virale (isolement et purification), Protéines de la matrice virale (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), Signaux de triage des protéines (génétique), Tensioactifs (pharmacologie), Vaccins à pseudo-particules virales (), Vaccins à pseudo-particules virales (métabolisme), Vésicules extracellulaires ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Bombyx, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Protéines d'insecte, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines recombinantes, Signaux de triage des protéines.
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Glycoprotéine de spicule des coronavirus, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines recombinantes.
- métabolisme : Bombyx, Dipeptidyl peptidase 4, Glycoprotéine de spicule des coronavirus, Hémolymphe, Larve, Matériaux biomimétiques, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines recombinantes, Vaccins à pseudo-particules virales.
- pharmacologie : Tensioactifs.
- Animaux, Humains, Infections à coronavirus, Lignée cellulaire, Matériaux biomimétiques, Vaccins à pseudo-particules virales, Vésicules extracellulaires.
English descriptors
- KwdEn :
- Animals, Biomimetic Materials (chemistry), Biomimetic Materials (metabolism), Bombyx (genetics), Bombyx (metabolism), Cell Line, Coronavirus Infections (diagnosis), Coronavirus Infections (prevention & control), Dipeptidyl Peptidase 4 (metabolism), Extracellular Vesicles (chemistry), Hemolymph (metabolism), Humans, Insect Proteins (genetics), Larva (metabolism), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Protein Sorting Signals (genetics), Recombinant Proteins (genetics), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (isolation & purification), Spike Glycoprotein, Coronavirus (metabolism), Surface-Active Agents (pharmacology), Vaccines, Virus-Like Particle (chemistry), Vaccines, Virus-Like Particle (drug effects), Vaccines, Virus-Like Particle (metabolism), Viral Envelope Proteins (genetics), Viral Envelope Proteins (isolation & purification), Viral Envelope Proteins (metabolism), Viral Matrix Proteins (genetics), Viral Matrix Proteins (isolation & purification), Viral Matrix Proteins (metabolism).
- MESH :
- chemical , chemistry : Vaccines, Virus-Like Particle.
- chemical , drug effects : Vaccines, Virus-Like Particle.
- chemical , genetics : Insect Proteins, Protein Sorting Signals, Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins, Viral Matrix Proteins.
- chemical , isolation & purification : Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins, Viral Matrix Proteins.
- chemical , metabolism : Dipeptidyl Peptidase 4, Recombinant Proteins, Spike Glycoprotein, Coronavirus, Vaccines, Virus-Like Particle, Viral Envelope Proteins, Viral Matrix Proteins.
- chemistry : Biomimetic Materials, Extracellular Vesicles.
- diagnosis : Coronavirus Infections.
- genetics : Bombyx, Middle East Respiratory Syndrome Coronavirus.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- metabolism : Biomimetic Materials, Bombyx, Hemolymph, Larva.
- chemical , pharmacology : Surface-Active Agents.
- prevention & control : Coronavirus Infections.
- Animals, Cell Line, Humans.
Abstract
Virus-like particle mimetic nanovesicles were prepared using insect cells. Surfactant treatment or mechanical extrusion make it possible to display the S protein on VLPs. Mechanical extrusion is more effective way to display the S protein on VLPs. S protein on the surface of nanovesicles were confirmed by immuno-TEM. The purified S protein (SοTM) has the ability to bind a receptor of MERS-CoV.
Url:
DOI: 10.1016/j.jbiotec.2019.10.007
PubMed: 31614169
PubMed Central: 7114102
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PMC:7114102Le document en format XML
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Park</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of 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(genetics)</term><term>Recombinant Proteins (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>Spike Glycoprotein, Coronavirus (genetics)</term><term>Spike Glycoprotein, Coronavirus (isolation & purification)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Surface-Active Agents (pharmacology)</term><term>Vaccines, Virus-Like Particle (chemistry)</term><term>Vaccines, Virus-Like Particle (drug effects)</term><term>Vaccines, Virus-Like Particle (metabolism)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (isolation & purification)</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Matrix Proteins (isolation & purification)</term><term>Viral Matrix Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bombyx (génétique)</term><term>Bombyx (métabolisme)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Dipeptidyl peptidase 4 (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus (génétique)</term><term>Glycoprotéine de spicule des coronavirus (isolement et purification)</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Hémolymphe (métabolisme)</term><term>Infections à coronavirus ()</term><term>Infections à coronavirus (diagnostic)</term><term>Larve (métabolisme)</term><term>Lignée cellulaire</term><term>Matériaux biomimétiques ()</term><term>Matériaux biomimétiques (métabolisme)</term><term>Protéines d'insecte (génétique)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (isolement et purification)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines de la matrice virale (génétique)</term><term>Protéines de la matrice virale (isolement et purification)</term><term>Protéines de la matrice virale (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines recombinantes (métabolisme)</term><term>Signaux de triage des protéines (génétique)</term><term>Tensioactifs (pharmacologie)</term><term>Vaccins à pseudo-particules virales ()</term><term>Vaccins à pseudo-particules virales (métabolisme)</term><term>Vésicules extracellulaires ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Vaccines, Virus-Like Particle</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Vaccines, Virus-Like Particle</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Insect Proteins</term><term>Protein Sorting Signals</term><term>Recombinant Proteins</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term><term>Recombinant Proteins</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccines, Virus-Like Particle</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Biomimetic Materials</term><term>Extracellular Vesicles</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bombyx</term><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bombyx</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Protéines d'insecte</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term><term>Signaux de triage des protéines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Biomimetic Materials</term><term>Bombyx</term><term>Hemolymph</term><term>Larva</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bombyx</term><term>Dipeptidyl peptidase 4</term><term>Glycoprotéine de spicule des coronavirus</term><term>Hémolymphe</term><term>Larve</term><term>Matériaux biomimétiques</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term><term>Vaccins à pseudo-particules virales</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Tensioactifs</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Surface-Active Agents</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Infections à coronavirus</term><term>Lignée cellulaire</term><term>Matériaux biomimétiques</term><term>Vaccins à pseudo-particules virales</term><term>Vésicules extracellulaires</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Virus-like particle mimetic nanovesicles were prepared using insect cells.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Surfactant treatment or mechanical extrusion make it possible to display the S protein on VLPs.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">Mechanical extrusion is more effective way to display the S protein on VLPs.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">S protein on the surface of nanovesicles were confirmed by immuno-TEM.</p></list-item><list-item id="lsti0025"><label>•</label><p id="par0025">The purified S protein (SοTM) has the ability to bind a receptor of MERS-CoV.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, J F" uniqKey="Chan J">J.F. Chan</name></author><author><name sortKey="Li, K S" uniqKey="Li K">K.S. Li</name></author><author><name sortKey="To, K K" uniqKey="To K">K.K. To</name></author><author><name sortKey="Cheng, V C" uniqKey="Cheng V">V.C. Cheng</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Charlton Hume, H K" uniqKey="Charlton Hume H">H.K. Charlton Hume</name></author><author><name sortKey="Vidigal, J" uniqKey="Vidigal J">J. Vidigal</name></author><author><name sortKey="Carrondo, M J T" uniqKey="Carrondo M">M.J.T. Carrondo</name></author><author><name sortKey="Middelberg, A P J" uniqKey="Middelberg A">A.P.J. Middelberg</name></author><author><name sortKey="Roldao, A" uniqKey="Roldao A">A. Roldão</name></author><author><name sortKey="Lua, L H L" uniqKey="Lua L">L.H.L. 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Chen</name></author></analytic></biblStruct></listBibl></div1></back></TEI><double pmid="31614169"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells</title><author><name sortKey="Kato, Tatsuya" sort="Kato, Tatsuya" uniqKey="Kato T" first="Tatsuya" last="Kato">Tatsuya Kato</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Takami, Yoshihiro" sort="Takami, Yoshihiro" uniqKey="Takami Y" first="Yoshihiro" last="Takami">Yoshihiro Takami</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Kumar Deo, Vipin" sort="Kumar Deo, Vipin" uniqKey="Kumar Deo V" first="Vipin" last="Kumar Deo">Vipin Kumar Deo</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Park, Enoch Y" sort="Park, Enoch Y" uniqKey="Park E" first="Enoch Y." last="Park">Enoch Y. Park</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31614169</idno><idno type="pmc">7114102</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114102</idno><idno type="RBID">PMC:7114102</idno><idno type="doi">10.1016/j.jbiotec.2019.10.007</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000D87</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D87</idno><idno type="wicri:Area/Pmc/Curation">000D87</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D87</idno><idno type="wicri:Area/Pmc/Checkpoint">000210</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000210</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells</title><author><name sortKey="Kato, Tatsuya" sort="Kato, Tatsuya" uniqKey="Kato T" first="Tatsuya" last="Kato">Tatsuya Kato</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Takami, Yoshihiro" sort="Takami, Yoshihiro" uniqKey="Takami Y" first="Yoshihiro" last="Takami">Yoshihiro Takami</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Kumar Deo, Vipin" sort="Kumar Deo, Vipin" uniqKey="Kumar Deo V" first="Vipin" last="Kumar Deo">Vipin Kumar Deo</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Park, Enoch Y" sort="Park, Enoch Y" uniqKey="Park E" first="Enoch Y." last="Park">Enoch Y. Park</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Biotechnology</title><idno type="ISSN">0168-1656</idno><idno type="eISSN">1873-4863</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Virus-like particle mimetic nanovesicles were prepared using insect cells.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Surfactant treatment or mechanical extrusion make it possible to display the S protein on VLPs.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">Mechanical extrusion is more effective way to display the S protein on VLPs.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">S protein on the surface of nanovesicles were confirmed by immuno-TEM.</p></list-item><list-item id="lsti0025"><label>•</label><p id="par0025">The purified S protein (SοTM) has the ability to bind a receptor of MERS-CoV.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, J F" uniqKey="Chan J">J.F. 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Chen</name></author></analytic></biblStruct></listBibl></div1></back></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells.</title><author><name sortKey="Kato, Tatsuya" sort="Kato, Tatsuya" uniqKey="Kato T" first="Tatsuya" last="Kato">Tatsuya Kato</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan; Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan; Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Takami, Yoshihiro" sort="Takami, Yoshihiro" uniqKey="Takami Y" first="Yoshihiro" last="Takami">Yoshihiro Takami</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Kumar Deo, Vipin" sort="Kumar Deo, Vipin" uniqKey="Kumar Deo V" first="Vipin" last="Kumar Deo">Vipin Kumar Deo</name><affiliation wicri:level="1"><nlm:affiliation>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Park, Enoch Y" sort="Park, Enoch Y" uniqKey="Park E" first="Enoch Y" last="Park">Enoch Y. Park</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan; Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan. Electronic address: park.enoch@shizuoka.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan; Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31614169</idno><idno type="pmid">31614169</idno><idno type="doi">10.1016/j.jbiotec.2019.10.007</idno><idno type="wicri:Area/PubMed/Corpus">000396</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000396</idno><idno type="wicri:Area/PubMed/Curation">000396</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000396</idno><idno type="wicri:Area/PubMed/Checkpoint">000421</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000421</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome coronavirus using insect cells.</title><author><name sortKey="Kato, Tatsuya" sort="Kato, Tatsuya" uniqKey="Kato T" first="Tatsuya" last="Kato">Tatsuya Kato</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan; Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan; Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Takami, Yoshihiro" sort="Takami, Yoshihiro" uniqKey="Takami Y" first="Yoshihiro" last="Takami">Yoshihiro Takami</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Kumar Deo, Vipin" sort="Kumar Deo, Vipin" uniqKey="Kumar Deo V" first="Vipin" last="Kumar Deo">Vipin Kumar Deo</name><affiliation wicri:level="1"><nlm:affiliation>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Office for International Collaborations, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Park, Enoch Y" sort="Park, Enoch Y" uniqKey="Park E" first="Enoch Y" last="Park">Enoch Y. Park</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan; Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan. Electronic address: park.enoch@shizuoka.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan; Green Chemistry Research Division, Research Institute of Green Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of biotechnology</title><idno type="eISSN">1873-4863</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biomimetic Materials (chemistry)</term><term>Biomimetic Materials (metabolism)</term><term>Bombyx (genetics)</term><term>Bombyx (metabolism)</term><term>Cell Line</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (prevention & control)</term><term>Dipeptidyl Peptidase 4 (metabolism)</term><term>Extracellular Vesicles (chemistry)</term><term>Hemolymph (metabolism)</term><term>Humans</term><term>Insect Proteins (genetics)</term><term>Larva (metabolism)</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Protein Sorting Signals (genetics)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>Spike Glycoprotein, Coronavirus (genetics)</term><term>Spike Glycoprotein, Coronavirus (isolation & purification)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Surface-Active Agents (pharmacology)</term><term>Vaccines, Virus-Like Particle (chemistry)</term><term>Vaccines, Virus-Like Particle (drug effects)</term><term>Vaccines, Virus-Like Particle (metabolism)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (isolation & purification)</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Matrix Proteins (isolation & purification)</term><term>Viral Matrix Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bombyx (génétique)</term><term>Bombyx (métabolisme)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Dipeptidyl peptidase 4 (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus (génétique)</term><term>Glycoprotéine de spicule des coronavirus (isolement et purification)</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Hémolymphe (métabolisme)</term><term>Infections à coronavirus ()</term><term>Infections à coronavirus (diagnostic)</term><term>Larve (métabolisme)</term><term>Lignée cellulaire</term><term>Matériaux biomimétiques ()</term><term>Matériaux biomimétiques (métabolisme)</term><term>Protéines d'insecte (génétique)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (isolement et purification)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines de la matrice virale (génétique)</term><term>Protéines de la matrice virale (isolement et purification)</term><term>Protéines de la matrice virale (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines recombinantes (métabolisme)</term><term>Signaux de triage des protéines (génétique)</term><term>Tensioactifs (pharmacologie)</term><term>Vaccins à pseudo-particules virales ()</term><term>Vaccins à pseudo-particules virales (métabolisme)</term><term>Vésicules extracellulaires ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Vaccines, Virus-Like Particle</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Vaccines, Virus-Like Particle</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Insect Proteins</term><term>Protein Sorting Signals</term><term>Recombinant Proteins</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term><term>Recombinant Proteins</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccines, Virus-Like Particle</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Biomimetic Materials</term><term>Extracellular Vesicles</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bombyx</term><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bombyx</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Protéines d'insecte</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term><term>Signaux de triage des protéines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Biomimetic Materials</term><term>Bombyx</term><term>Hemolymph</term><term>Larva</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bombyx</term><term>Dipeptidyl peptidase 4</term><term>Glycoprotéine de spicule des coronavirus</term><term>Hémolymphe</term><term>Larve</term><term>Matériaux biomimétiques</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term><term>Vaccins à pseudo-particules virales</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Tensioactifs</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Surface-Active Agents</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Infections à coronavirus</term><term>Lignée cellulaire</term><term>Matériaux biomimétiques</term><term>Vaccins à pseudo-particules virales</term><term>Vésicules extracellulaires</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) first emerged in 2012, and over 2000 infections and 800 deaths have been confirmed in 27 countries. However, to date, no commercial vaccine is available. In this study, structural proteins of MERS-CoV were expressed in silkworm larvae and Bm5 cells for the development of vaccine candidates against MERS-CoV and diagnostic methods. The spike (S) protein of MERS-CoV lacking its transmembrane and cytoplasmic domains (SΔTM) was secreted into the hemolymph of silkworm larvae using a bombyxin signal peptide and purified using affinity chromatography. The purified SΔTM forms small nanoparticles as well as the full-length S protein and has the ability to bind human dipeptidyl peptidase 4 (DPP4), which is a receptor of MERS-CoV. These results indicate that bioactive SΔTM was expressed in silkworm larvae. To produce MERS-CoV-like particles (MERS-CoV-LPs), the coexpression of spike proteins was performed in Bm5 cells and envelope (E) and membrane (M) proteins secreted E and M proteins extracellularly, suggesting that MERS-CoV-LPs may be formed. However, this S protein was not displayed on virus-like particles (VLPs) even though E and M proteins were secreted into the culture supernatant. By surfactant treatment and mechanical extrusion using S protein- or three structural protein-expressing Bm5 cells, S protein-displaying nanovesicles with diameters of approximately 100-200 nm were prepared and confirmed by immuno-TEM. The mechanical extrusion method is favorable for obtaining uniform recombinant protein-displaying nanovesicles from cultured cells. The purified SΔTM from silkworm larvae and S protein-displaying nanovesicles from Bm5 cells may lead to the development of nanoparticle-based vaccines against MERS-CoV and the diagnostic detection of MERS-CoV.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:31614169" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |