Remote Laboratory Management: Respiratory Virus Diagnostics.
Identifieur interne : 002197 ( Ncbi/Merge ); précédent : 002196; suivant : 002198Remote Laboratory Management: Respiratory Virus Diagnostics.
Auteurs : Elena V. Petrova [États-Unis] ; Vasanthi Avadhanula [États-Unis] ; Sarah Michel [États-Unis] ; Karen E. Gincoo [États-Unis] ; Pedro A. Piedra [États-Unis] ; Sharmila Anandasabapathy [États-Unis]Source :
- Journal of visualized experiments : JoVE [ 1940-087X ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Grippe humaine.
- génétique : Orthomyxoviridae.
- Humains, Laboratoires, Réaction de polymérisation en chaine en temps réel.
English descriptors
- KwdEn :
- MESH :
- diagnosis : Influenza, Human.
- genetics : Orthomyxoviridae.
- Humans, Laboratories, Real-Time Polymerase Chain Reaction.
Abstract
An uptick in recent pandemics (Ebola, Zika, MERS, influenza, etc.) underlines the need for a more 'nimble,' coordinated response that addresses a multitude of issues ranging from transportation, access, facilities, equipment, and communication to provider training. To address this need, we have developed an innovative, scalable, logistics-enhanced, mobile, laboratory facility for emergencies and epidemics in resource-constrained global settings. Utilizing a background in clinical operations as an academic medical center, we designed a rapidly-deployable, modular BSL-2 and BSL-3 facility with user-friendly software for tracking and management of drugs and supplies in remote regions during epidemics and outbreaks. Here, we present our intermodal, mobile, expandable shipping-container laboratory units. The design of the laboratory facilitates off-grid usage by minimizing power consumption and allowing alternate water sources. The unit's information communication technology (ICT) platform provides (i) user-friendly tablet-based documentation, (ii) enhanced tracking of patients and supplies, and (iii) integrated communication onsite with built-in telehealth capabilities. To ensure quality in remote environments, we have developed a checklist for a basic laboratory workflow and a protocol for respiratory viral diagnosis using reverse-transcription polymerase chain reaction (RT-PCR). As described, this innovative and comprehensive approach allows for the provision of laboratory capability in resource-limited global environments.
DOI: 10.3791/59188
PubMed: 31009009
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pubmed:31009009Le document en format XML
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Petrova</name><affiliation wicri:level="4"><nlm:affiliation>Baylor Global Health, Baylor College of Medicine; Department of Medicine, Baylor College of Medicine.</nlm:affiliation><country>États-Unis</country><placeName><settlement type="city">Houston</settlement><region type="state">Texas</region></placeName><orgName type="university">Baylor College of Medicine</orgName></affiliation></author><author><name sortKey="Avadhanula, Vasanthi" sort="Avadhanula, Vasanthi" uniqKey="Avadhanula V" first="Vasanthi" last="Avadhanula">Vasanthi Avadhanula</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular Virology and Microbiology, Baylor College of Medicine.</nlm:affiliation><country>États-Unis</country><placeName><settlement type="city">Houston</settlement><region type="state">Texas</region></placeName><orgName type="university">Baylor College of Medicine</orgName></affiliation></author><author><name sortKey="Michel, Sarah" sort="Michel, Sarah" uniqKey="Michel S" first="Sarah" last="Michel">Sarah Michel</name><affiliation wicri:level="4"><nlm:affiliation>Baylor Global Health, Baylor College of Medicine.</nlm:affiliation><country>États-Unis</country><placeName><settlement type="city">Houston</settlement><region type="state">Texas</region></placeName><orgName type="university">Baylor College of Medicine</orgName></affiliation></author><author><name sortKey="Gincoo, Karen E" sort="Gincoo, Karen E" uniqKey="Gincoo K" first="Karen E" last="Gincoo">Karen E. Gincoo</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular Virology and Microbiology, Baylor College of Medicine.</nlm:affiliation><country>États-Unis</country><placeName><settlement type="city">Houston</settlement><region type="state">Texas</region></placeName><orgName type="university">Baylor College of Medicine</orgName></affiliation></author><author><name sortKey="Piedra, Pedro A" sort="Piedra, Pedro A" uniqKey="Piedra P" first="Pedro A" last="Piedra">Pedro A. Piedra</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular Virology and Microbiology, Baylor College of Medicine.</nlm:affiliation><country>États-Unis</country><placeName><settlement type="city">Houston</settlement><region type="state">Texas</region></placeName><orgName type="university">Baylor College of Medicine</orgName></affiliation></author><author><name sortKey="Anandasabapathy, Sharmila" sort="Anandasabapathy, Sharmila" uniqKey="Anandasabapathy S" first="Sharmila" last="Anandasabapathy">Sharmila Anandasabapathy</name><affiliation wicri:level="1"><nlm:affiliation>Baylor Global Health, Baylor College of Medicine; Department of Medicine, Baylor College of Medicine; sharmila.anandasabapathy@bcm.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Baylor Global Health, Baylor College of Medicine; Department of Medicine</wicri:regionArea><wicri:noRegion>Baylor College of Medicine; Department of Medicine</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of visualized experiments : JoVE</title><idno type="eISSN">1940-087X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Influenza, Human (diagnosis)</term><term>Laboratories</term><term>Orthomyxoviridae (genetics)</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Grippe humaine (diagnostic)</term><term>Humains</term><term>Laboratoires</term><term>Orthomyxoviridae (génétique)</term><term>Réaction de polymérisation en chaine en temps réel</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Laboratories</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Laboratoires</term><term>Réaction de polymérisation en chaine en temps réel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An uptick in recent pandemics (Ebola, Zika, MERS, influenza, etc.) underlines the need for a more 'nimble,' coordinated response that addresses a multitude of issues ranging from transportation, access, facilities, equipment, and communication to provider training. To address this need, we have developed an innovative, scalable, logistics-enhanced, mobile, laboratory facility for emergencies and epidemics in resource-constrained global settings. Utilizing a background in clinical operations as an academic medical center, we designed a rapidly-deployable, modular BSL-2 and BSL-3 facility with user-friendly software for tracking and management of drugs and supplies in remote regions during epidemics and outbreaks. Here, we present our intermodal, mobile, expandable shipping-container laboratory units. The design of the laboratory facilitates off-grid usage by minimizing power consumption and allowing alternate water sources. The unit's information communication technology (ICT) platform provides (i) user-friendly tablet-based documentation, (ii) enhanced tracking of patients and supplies, and (iii) integrated communication onsite with built-in telehealth capabilities. To ensure quality in remote environments, we have developed a checklist for a basic laboratory workflow and a protocol for respiratory viral diagnosis using reverse-transcription polymerase chain reaction (RT-PCR). As described, this innovative and comprehensive approach allows for the provision of laboratory capability in resource-limited global environments.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31009009</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>20</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>20</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1940-087X</ISSN><JournalIssue CitedMedium="Internet"><Issue>146</Issue><PubDate><Year>2019</Year><Month>04</Month><Day>06</Day></PubDate></JournalIssue><Title>Journal of visualized experiments : JoVE</Title><ISOAbbreviation>J Vis Exp</ISOAbbreviation></Journal><ArticleTitle>Remote Laboratory Management: Respiratory Virus Diagnostics.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.3791/59188</ELocationID><Abstract><AbstractText>An uptick in recent pandemics (Ebola, Zika, MERS, influenza, etc.) underlines the need for a more 'nimble,' coordinated response that addresses a multitude of issues ranging from transportation, access, facilities, equipment, and communication to provider training. To address this need, we have developed an innovative, scalable, logistics-enhanced, mobile, laboratory facility for emergencies and epidemics in resource-constrained global settings. Utilizing a background in clinical operations as an academic medical center, we designed a rapidly-deployable, modular BSL-2 and BSL-3 facility with user-friendly software for tracking and management of drugs and supplies in remote regions during epidemics and outbreaks. Here, we present our intermodal, mobile, expandable shipping-container laboratory units. The design of the laboratory facilitates off-grid usage by minimizing power consumption and allowing alternate water sources. The unit's information communication technology (ICT) platform provides (i) user-friendly tablet-based documentation, (ii) enhanced tracking of patients and supplies, and (iii) integrated communication onsite with built-in telehealth capabilities. To ensure quality in remote environments, we have developed a checklist for a basic laboratory workflow and a protocol for respiratory viral diagnosis using reverse-transcription polymerase chain reaction (RT-PCR). As described, this innovative and comprehensive approach allows for the provision of laboratory capability in resource-limited global environments.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Petrova</LastName><ForeName>Elena V</ForeName><Initials>EV</Initials><AffiliationInfo><Affiliation>Baylor Global Health, Baylor College of Medicine; Department of Medicine, Baylor College of Medicine.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Avadhanula</LastName><ForeName>Vasanthi</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Department of Molecular Virology and Microbiology, Baylor College of Medicine.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Michel</LastName><ForeName>Sarah</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Baylor Global Health, Baylor College of Medicine.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gincoo</LastName><ForeName>Karen E</ForeName><Initials>KE</Initials><AffiliationInfo><Affiliation>Department of Molecular Virology and Microbiology, Baylor College of Medicine.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Piedra</LastName><ForeName>Pedro A</ForeName><Initials>PA</Initials><AffiliationInfo><Affiliation>Department of Molecular Virology and Microbiology, Baylor College of Medicine.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Anandasabapathy</LastName><ForeName>Sharmila</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Baylor Global Health, Baylor College of Medicine; Department of Medicine, Baylor College of Medicine; sharmila.anandasabapathy@bcm.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D059040">Video-Audio Media</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>04</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Vis Exp</MedlineTA><NlmUniqueID>101313252</NlmUniqueID><ISSNLinking>1940-087X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007753" MajorTopicYN="Y">Laboratories</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009975" MajorTopicYN="N">Orthomyxoviridae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>4</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>4</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31009009</ArticleId><ArticleId IdType="doi">10.3791/59188</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region><settlement><li>Houston</li></settlement><orgName><li>Baylor College of Medicine</li></orgName></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Petrova, Elena V" sort="Petrova, Elena V" uniqKey="Petrova E" first="Elena V" last="Petrova">Elena V. Petrova</name></region><name sortKey="Anandasabapathy, Sharmila" sort="Anandasabapathy, Sharmila" uniqKey="Anandasabapathy S" first="Sharmila" last="Anandasabapathy">Sharmila Anandasabapathy</name><name sortKey="Avadhanula, Vasanthi" sort="Avadhanula, Vasanthi" uniqKey="Avadhanula V" first="Vasanthi" last="Avadhanula">Vasanthi Avadhanula</name><name sortKey="Gincoo, Karen E" sort="Gincoo, Karen E" uniqKey="Gincoo K" first="Karen E" last="Gincoo">Karen E. Gincoo</name><name sortKey="Michel, Sarah" sort="Michel, Sarah" uniqKey="Michel S" first="Sarah" last="Michel">Sarah Michel</name><name sortKey="Piedra, Pedro A" sort="Piedra, Pedro A" uniqKey="Piedra P" first="Pedro A" last="Piedra">Pedro A. Piedra</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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