Xrep, a plasmid-stimulating X chromosomal sequence bearing similarities to the BK virus replication origin and viral enhancers.
Identifieur interne : 001F77 ( Ncbi/Merge ); précédent : 001F76; suivant : 001F78Xrep, a plasmid-stimulating X chromosomal sequence bearing similarities to the BK virus replication origin and viral enhancers.
Auteurs : D E Riley ; R. Reeves ; S M GartlerSource :
- Nucleic acids research [ 0305-1048 ] ; 1986.
Descripteurs français
- KwdFr :
- ADN viral (analyse), Acetyltransferases (génétique), Cartographie chromosomique, Chloramphenicol O-acetyltransferase, Chromosome X, Clonage moléculaire, Délétion de segment de chromosome, Escherichia coli (génétique), Gènes régulateurs, Humains, Plasmides, Polyomavirus (génétique), Réplication virale, Saccharomyces cerevisiae (génétique), Similitude de séquences d'acides nucléiques, Séquence nucléotidique, Virus BK (génétique), Éléments activateurs (génétique).
- MESH :
- analyse : ADN viral.
- génétique : Acetyltransferases, Escherichia coli, Polyomavirus, Saccharomyces cerevisiae, Virus BK.
- Cartographie chromosomique, Chloramphenicol O-acetyltransferase, Chromosome X, Clonage moléculaire, Délétion de segment de chromosome, Gènes régulateurs, Humains, Plasmides, Réplication virale, Similitude de séquences d'acides nucléiques, Séquence nucléotidique, Éléments activateurs (génétique).
English descriptors
- KwdEn :
- Acetyltransferases (genetics), BK Virus (genetics), Base Sequence, Chloramphenicol O-Acetyltransferase, Chromosome Deletion, Chromosome Mapping, Cloning, Molecular, DNA, Viral (analysis), Enhancer Elements, Genetic, Escherichia coli (genetics), Genes, Regulator, Humans, Plasmids, Polyomavirus (genetics), Saccharomyces cerevisiae (genetics), Sequence Homology, Nucleic Acid, Virus Replication, X Chromosome.
- MESH :
- chemical , analysis : DNA, Viral.
- chemical , genetics : Acetyltransferases.
- genetics : BK Virus, Escherichia coli, Polyomavirus, Saccharomyces cerevisiae.
- Base Sequence, Chloramphenicol O-Acetyltransferase, Chromosome Deletion, Chromosome Mapping, Cloning, Molecular, Enhancer Elements, Genetic, Genes, Regulator, Humans, Plasmids, Sequence Homology, Nucleic Acid, Virus Replication, X Chromosome.
Abstract
The human X chromosome-linked fragment, "Xrep," was sequenced because it exerts a positive effect on plasmid growth in both E. coli and Saccharomyces cerevisiae. The sequence revealed three features similar to the human BK virus replication origin: Xrep has a true palindrome, CCTCC(T)3CCTCC, which is similar to "true" palindrome-like sequences found at the replication origins of polyoma [CCTC(T/C)10CTCC], BK [CCTC(A/G)8CCTCC] and SV40 [CCTCC(A)6GCCTCC] viruses. Twenty nucleotides away from the true palindrome, Xrep has the sequence GAATCCTATTCACTTTT while BK virus, the human analogue of SV40, has GAAATCCCTATTCTTTT in exactly the same position relative to the true palindrome. These two 17-mers differ only in the positions of two nucleotides comparing Xrep and BK virus. Also similar to the replication origins of DNA viruses, Xrep appears to have a cluster of enhancers adjacent to the origin-like sequences. Potent enhancer-like activity was detected in pSV1 X CAT/Xrep constructs. Xrep may originate from an endogenous virus, or from an X chromosomal replication origin.
DOI: 10.1093/nar/14.23.9407
PubMed: 3025813
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002A79
- to stream PubMed, to step Curation: 002A79
- to stream PubMed, to step Checkpoint: 002921
Links to Exploration step
pubmed:3025813Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetyltransferases (genetics)</term>
<term>BK Virus (genetics)</term>
<term>Base Sequence</term>
<term>Chloramphenicol O-Acetyltransferase</term>
<term>Chromosome Deletion</term>
<term>Chromosome Mapping</term>
<term>Cloning, Molecular</term>
<term>DNA, Viral (analysis)</term>
<term>Enhancer Elements, Genetic</term>
<term>Escherichia coli (genetics)</term>
<term>Genes, Regulator</term>
<term>Humans</term>
<term>Plasmids</term>
<term>Polyomavirus (genetics)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Sequence Homology, Nucleic Acid</term>
<term>Virus Replication</term>
<term>X Chromosome</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (analyse)</term>
<term>Acetyltransferases (génétique)</term>
<term>Cartographie chromosomique</term>
<term>Chloramphenicol O-acetyltransferase</term>
<term>Chromosome X</term>
<term>Clonage moléculaire</term>
<term>Délétion de segment de chromosome</term>
<term>Escherichia coli (génétique)</term>
<term>Gènes régulateurs</term>
<term>Humains</term>
<term>Plasmides</term>
<term>Polyomavirus (génétique)</term>
<term>Réplication virale</term>
<term>Saccharomyces cerevisiae (génétique)</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>Séquence nucléotidique</term>
<term>Virus BK (génétique)</term>
<term>Éléments activateurs (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Acetyltransferases</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>BK Virus</term>
<term>Escherichia coli</term>
<term>Polyomavirus</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Acetyltransferases</term>
<term>Escherichia coli</term>
<term>Polyomavirus</term>
<term>Saccharomyces cerevisiae</term>
<term>Virus BK</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Chloramphenicol O-Acetyltransferase</term>
<term>Chromosome Deletion</term>
<term>Chromosome Mapping</term>
<term>Cloning, Molecular</term>
<term>Enhancer Elements, Genetic</term>
<term>Genes, Regulator</term>
<term>Humans</term>
<term>Plasmids</term>
<term>Sequence Homology, Nucleic Acid</term>
<term>Virus Replication</term>
<term>X Chromosome</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Cartographie chromosomique</term>
<term>Chloramphenicol O-acetyltransferase</term>
<term>Chromosome X</term>
<term>Clonage moléculaire</term>
<term>Délétion de segment de chromosome</term>
<term>Gènes régulateurs</term>
<term>Humains</term>
<term>Plasmides</term>
<term>Réplication virale</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>Séquence nucléotidique</term>
<term>Éléments activateurs (génétique)</term>
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<front><div type="abstract" xml:lang="en">The human X chromosome-linked fragment, "Xrep," was sequenced because it exerts a positive effect on plasmid growth in both E. coli and Saccharomyces cerevisiae. The sequence revealed three features similar to the human BK virus replication origin: Xrep has a true palindrome, CCTCC(T)3CCTCC, which is similar to "true" palindrome-like sequences found at the replication origins of polyoma [CCTC(T/C)10CTCC], BK [CCTC(A/G)8CCTCC] and SV40 [CCTCC(A)6GCCTCC] viruses. Twenty nucleotides away from the true palindrome, Xrep has the sequence GAATCCTATTCACTTTT while BK virus, the human analogue of SV40, has GAAATCCCTATTCTTTT in exactly the same position relative to the true palindrome. These two 17-mers differ only in the positions of two nucleotides comparing Xrep and BK virus. Also similar to the replication origins of DNA viruses, Xrep appears to have a cluster of enhancers adjacent to the origin-like sequences. Potent enhancer-like activity was detected in pSV1 X CAT/Xrep constructs. Xrep may originate from an endogenous virus, or from an X chromosomal replication origin.</div>
</front>
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<Title>Nucleic acids research</Title>
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<ArticleTitle>Xrep, a plasmid-stimulating X chromosomal sequence bearing similarities to the BK virus replication origin and viral enhancers.</ArticleTitle>
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<Abstract><AbstractText>The human X chromosome-linked fragment, "Xrep," was sequenced because it exerts a positive effect on plasmid growth in both E. coli and Saccharomyces cerevisiae. The sequence revealed three features similar to the human BK virus replication origin: Xrep has a true palindrome, CCTCC(T)3CCTCC, which is similar to "true" palindrome-like sequences found at the replication origins of polyoma [CCTC(T/C)10CTCC], BK [CCTC(A/G)8CCTCC] and SV40 [CCTCC(A)6GCCTCC] viruses. Twenty nucleotides away from the true palindrome, Xrep has the sequence GAATCCTATTCACTTTT while BK virus, the human analogue of SV40, has GAAATCCCTATTCTTTT in exactly the same position relative to the true palindrome. These two 17-mers differ only in the positions of two nucleotides comparing Xrep and BK virus. Also similar to the replication origins of DNA viruses, Xrep appears to have a cluster of enhancers adjacent to the origin-like sequences. Potent enhancer-like activity was detected in pSV1 X CAT/Xrep constructs. Xrep may originate from an endogenous virus, or from an X chromosomal replication origin.</AbstractText>
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<name sortKey="Reeves, R" sort="Reeves, R" uniqKey="Reeves R" first="R" last="Reeves">R. Reeves</name>
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{{Explor lien |wiki= Sante |area= MersV1 |flux= Ncbi |étape= Merge |type= RBID |clé= pubmed:3025813 |texte= Xrep, a plasmid-stimulating X chromosomal sequence bearing similarities to the BK virus replication origin and viral enhancers. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:3025813" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
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