Heterologous prime–boost vaccination with adenoviral vector and protein nanoparticles induces both Th1 and Th2 responses against Middle East respiratory syndrome coronavirus
Identifieur interne : 001E22 ( Ncbi/Merge ); précédent : 001E21; suivant : 001E23Heterologous prime–boost vaccination with adenoviral vector and protein nanoparticles induces both Th1 and Th2 responses against Middle East respiratory syndrome coronavirus
Auteurs : Seo-Yeon Jung [Corée du Sud] ; Kyung Won Kang [Corée du Sud] ; Eun-Young Lee [Corée du Sud] ; Dong-Won Seo [Corée du Sud] ; Hong-Lim Kim [Corée du Sud] ; Hak Kim [Corée du Sud] ; Taewoo Kwon [Corée du Sud] ; Hye-Lim Park [Corée du Sud] ; Hun Kim [Corée du Sud] ; Sang-Myeong Lee [Corée du Sud] ; Jae-Hwan Nam [Corée du Sud]Source :
- Vaccine [ 0264-410X ] ; 2018.
Descripteurs français
- KwdFr :
- Activation des lymphocytes (), Adjuvants immunologiques (administration et posologie), Adénovirus humains (génétique), Adénovirus humains (immunologie), Alun (administration et posologie), Animaux, Anticorps antiviraux (biosynthèse), Anticorps neutralisants (biosynthèse), Calendrier vaccinal, Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Femelle, Glycoprotéine de spicule des coronavirus (administration et posologie), Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (immunologie), Humains, Immunoglobuline G (biosynthèse), Immunogénicité des vaccins, Infections à coronavirus (), Infections à coronavirus (immunologie), Infections à coronavirus (virologie), Lymphocytes auxiliaires Th1 (), Lymphocytes auxiliaires Th1 (immunologie), Lymphocytes auxiliaires Th1 (virologie), Lymphocytes auxiliaires Th2 (), Lymphocytes auxiliaires Th2 (immunologie), Lymphocytes auxiliaires Th2 (virologie), Nanoparticules (), Nanoparticules (administration et posologie), Rappel de vaccin (), Souris, Souris de lignée BALB C, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie).
- MESH :
- administration et posologie : Adjuvants immunologiques, Alun, Glycoprotéine de spicule des coronavirus, Nanoparticules, Vaccins antiviraux.
- biosynthèse : Anticorps antiviraux, Anticorps neutralisants, Immunoglobuline G.
- génétique : Adénovirus humains, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Vaccins antiviraux.
- immunologie : Adénovirus humains, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Infections à coronavirus, Lymphocytes auxiliaires Th1, Lymphocytes auxiliaires Th2, Vaccins antiviraux.
- virologie : Infections à coronavirus, Lymphocytes auxiliaires Th1, Lymphocytes auxiliaires Th2.
- Activation des lymphocytes, Animaux, Calendrier vaccinal, Coronavirus du syndrome respiratoire du Moyen-Orient, Femelle, Humains, Immunogénicité des vaccins, Infections à coronavirus, Lymphocytes auxiliaires Th1, Lymphocytes auxiliaires Th2, Nanoparticules, Rappel de vaccin, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Adenoviruses, Human (genetics), Adenoviruses, Human (immunology), Adjuvants, Immunologic (administration & dosage), Alum Compounds (administration & dosage), Animals, Antibodies, Neutralizing (biosynthesis), Antibodies, Viral (biosynthesis), Coronavirus Infections (immunology), Coronavirus Infections (prevention & control), Coronavirus Infections (virology), Female, Humans, Immunization Schedule, Immunization, Secondary (methods), Immunogenicity, Vaccine, Immunoglobulin G (biosynthesis), Lymphocyte Activation (drug effects), Mice, Mice, Inbred BALB C, Middle East Respiratory Syndrome Coronavirus (drug effects), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Nanoparticles (administration & dosage), Nanoparticles (chemistry), Spike Glycoprotein, Coronavirus (administration & dosage), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (immunology), Th1 Cells (drug effects), Th1 Cells (immunology), Th1 Cells (virology), Th2 Cells (drug effects), Th2 Cells (immunology), Th2 Cells (virology), Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Adjuvants, Immunologic, Alum Compounds, Spike Glycoprotein, Coronavirus, Viral Vaccines.
- chemical , biosynthesis : Antibodies, Neutralizing, Antibodies, Viral, Immunoglobulin G.
- administration & dosage : Nanoparticles.
- chemistry : Nanoparticles.
- drug effects : Lymphocyte Activation, Middle East Respiratory Syndrome Coronavirus, Th1 Cells, Th2 Cells.
- genetics : Adenoviruses, Human, Middle East Respiratory Syndrome Coronavirus, Spike Glycoprotein, Coronavirus, Viral Vaccines.
- immunology : Adenoviruses, Human, Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus, Spike Glycoprotein, Coronavirus, Th1 Cells, Th2 Cells, Viral Vaccines.
- methods : Immunization, Secondary.
- prevention & control : Coronavirus Infections.
- virology : Coronavirus Infections, Th1 Cells, Th2 Cells.
- Animals, Female, Humans, Immunization Schedule, Immunogenicity, Vaccine, Mice, Mice, Inbred BALB C.
Abstract
Immunization with MERS spike protein nanoparticles induced only Th2-biased response. Heterologous prime-boost immunization induced both Th1 and Th2-biased responses. Our vaccination strategy showed the protective effect against MERS-CoV.
Url:
DOI: 10.1016/j.vaccine.2018.04.082
PubMed: 29739720
PubMed Central: 7115429
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PMC:7115429Le document en format XML
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(virology)</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (genetics)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes ()</term><term>Adjuvants immunologiques (administration et posologie)</term><term>Adénovirus humains (génétique)</term><term>Adénovirus humains (immunologie)</term><term>Alun (administration et posologie)</term><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Anticorps neutralisants (biosynthèse)</term><term>Calendrier vaccinal</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus (administration et posologie)</term><term>Glycoprotéine de spicule des coronavirus 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xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lymphocyte Activation</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Th1 Cells</term><term>Th2 Cells</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviruses, Human</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adénovirus humains</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Adénovirus humains</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Infections à coronavirus</term><term>Lymphocytes auxiliaires Th1</term><term>Lymphocytes auxiliaires Th2</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Adenoviruses, Human</term><term>Coronavirus Infections</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Spike Glycoprotein, Coronavirus</term><term>Th1 Cells</term><term>Th2 Cells</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunization, Secondary</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Lymphocytes auxiliaires Th1</term><term>Lymphocytes auxiliaires Th2</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Th1 Cells</term><term>Th2 Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Humans</term><term>Immunization Schedule</term><term>Immunogenicity, Vaccine</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Calendrier vaccinal</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Femelle</term><term>Humains</term><term>Immunogénicité des vaccins</term><term>Infections à coronavirus</term><term>Lymphocytes auxiliaires Th1</term><term>Lymphocytes auxiliaires Th2</term><term>Nanoparticules</term><term>Rappel de vaccin</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="l0005"><list-item id="o0005"><label>•</label><p id="p0005">Immunization with MERS spike protein nanoparticles induced only Th2-biased response.</p></list-item><list-item id="o0010"><label>•</label><p id="p0010">Heterologous prime-boost immunization induced both Th1 and Th2-biased responses.</p></list-item><list-item id="o0015"><label>•</label><p id="p9708">Our vaccination strategy showed the protective effect against MERS-CoV.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, J F W" uniqKey="Chan J">J.F.W. 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Varga</name></author></analytic></biblStruct></listBibl></div1></back></TEI><double pmid="29739720"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Heterologous prime–boost vaccination with adenoviral vector and protein nanoparticles induces both Th1 and Th2 responses against Middle East respiratory syndrome coronavirus</title><author><name sortKey="Jung, Seo Yeon" sort="Jung, Seo Yeon" uniqKey="Jung S" first="Seo-Yeon" last="Jung">Seo-Yeon Jung</name><affiliation wicri:level="1"><nlm:aff id="af005">Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Kang, Kyung Won" sort="Kang, Kyung Won" uniqKey="Kang K" first="Kyung Won" last="Kang">Kyung Won Kang</name><affiliation wicri:level="1"><nlm:aff id="af010">Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Eun Young" sort="Lee, Eun Young" uniqKey="Lee E" first="Eun-Young" last="Lee">Eun-Young Lee</name><affiliation wicri:level="1"><nlm:aff id="af005">Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Seo, Dong Won" sort="Seo, Dong Won" uniqKey="Seo D" first="Dong-Won" last="Seo">Dong-Won Seo</name><affiliation wicri:level="1"><nlm:aff id="af010">Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hong Lim" sort="Kim, Hong Lim" uniqKey="Kim H" first="Hong-Lim" last="Kim">Hong-Lim Kim</name><affiliation wicri:level="1"><nlm:aff id="af015">Seoul St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Seoul St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Seoul 06591</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Hak" sort="Kim, Hak" uniqKey="Kim H" first="Hak" last="Kim">Hak Kim</name><affiliation wicri:level="1"><nlm:aff id="af020">Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Kwon, Taewoo" sort="Kwon, Taewoo" uniqKey="Kwon T" first="Taewoo" last="Kwon">Taewoo Kwon</name><affiliation wicri:level="1"><nlm:aff id="af020">Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Park, Hye Lim" sort="Park, Hye Lim" uniqKey="Park H" first="Hye-Lim" last="Park">Hye-Lim Park</name><affiliation wicri:level="1"><nlm:aff id="af005">Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hun" sort="Kim, Hun" uniqKey="Kim H" first="Hun" last="Kim">Hun Kim</name><affiliation wicri:level="1"><nlm:aff id="af020">Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sang Myeong" sort="Lee, Sang Myeong" uniqKey="Lee S" first="Sang-Myeong" last="Lee">Sang-Myeong Lee</name><affiliation wicri:level="1"><nlm:aff id="af010">Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name><affiliation wicri:level="1"><nlm:aff id="af005">Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">29739720</idno><idno type="pmc">7115429</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115429</idno><idno type="RBID">PMC:7115429</idno><idno type="doi">10.1016/j.vaccine.2018.04.082</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">001171</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001171</idno><idno type="wicri:Area/Pmc/Curation">001171</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001171</idno><idno type="wicri:Area/Pmc/Checkpoint">000569</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000569</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Heterologous prime–boost vaccination with adenoviral vector and protein nanoparticles induces both Th1 and Th2 responses against Middle East respiratory syndrome coronavirus</title><author><name sortKey="Jung, Seo Yeon" sort="Jung, Seo Yeon" uniqKey="Jung S" first="Seo-Yeon" last="Jung">Seo-Yeon Jung</name><affiliation wicri:level="1"><nlm:aff id="af005">Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Kang, Kyung Won" sort="Kang, Kyung Won" uniqKey="Kang K" first="Kyung Won" last="Kang">Kyung Won Kang</name><affiliation wicri:level="1"><nlm:aff id="af010">Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Eun Young" sort="Lee, Eun Young" uniqKey="Lee E" first="Eun-Young" last="Lee">Eun-Young Lee</name><affiliation wicri:level="1"><nlm:aff id="af005">Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Seo, Dong Won" sort="Seo, Dong Won" uniqKey="Seo D" first="Dong-Won" last="Seo">Dong-Won Seo</name><affiliation wicri:level="1"><nlm:aff id="af010">Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hong Lim" sort="Kim, Hong Lim" uniqKey="Kim H" first="Hong-Lim" last="Kim">Hong-Lim Kim</name><affiliation wicri:level="1"><nlm:aff id="af015">Seoul St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Seoul St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Seoul 06591</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Hak" sort="Kim, Hak" uniqKey="Kim H" first="Hak" last="Kim">Hak Kim</name><affiliation wicri:level="1"><nlm:aff id="af020">Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Kwon, Taewoo" sort="Kwon, Taewoo" uniqKey="Kwon T" first="Taewoo" last="Kwon">Taewoo Kwon</name><affiliation wicri:level="1"><nlm:aff id="af020">Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Park, Hye Lim" sort="Park, Hye Lim" uniqKey="Park H" first="Hye-Lim" last="Park">Hye-Lim Park</name><affiliation wicri:level="1"><nlm:aff id="af005">Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hun" sort="Kim, Hun" uniqKey="Kim H" first="Hun" last="Kim">Hun Kim</name><affiliation wicri:level="1"><nlm:aff id="af020">Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sang Myeong" sort="Lee, Sang Myeong" uniqKey="Lee S" first="Sang-Myeong" last="Lee">Sang-Myeong Lee</name><affiliation wicri:level="1"><nlm:aff id="af010">Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name><affiliation wicri:level="1"><nlm:aff id="af005">Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="l0005"><list-item id="o0005"><label>•</label><p id="p0005">Immunization with MERS spike protein nanoparticles induced only Th2-biased response.</p></list-item><list-item id="o0010"><label>•</label><p id="p0010">Heterologous prime-boost immunization induced both Th1 and Th2-biased responses.</p></list-item><list-item id="o0015"><label>•</label><p id="p9708">Our vaccination strategy showed the protective effect against MERS-CoV.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, J F W" uniqKey="Chan J">J.F.W. 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wicri:level="1"><nlm:affiliation>Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Eun Young" sort="Lee, Eun Young" uniqKey="Lee E" first="Eun-Young" last="Lee">Eun-Young Lee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Seo, Dong Won" sort="Seo, Dong Won" uniqKey="Seo D" first="Dong-Won" last="Seo">Dong-Won Seo</name><affiliation wicri:level="1"><nlm:affiliation>Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hong Lim" sort="Kim, Hong Lim" uniqKey="Kim H" first="Hong-Lim" last="Kim">Hong-Lim Kim</name><affiliation wicri:level="1"><nlm:affiliation>Seoul St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Seoul St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Seoul 06591</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Hak" sort="Kim, Hak" uniqKey="Kim H" first="Hak" last="Kim">Hak Kim</name><affiliation wicri:level="1"><nlm:affiliation>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Kwon, Taewoo" sort="Kwon, Taewoo" uniqKey="Kwon T" first="Taewoo" last="Kwon">Taewoo Kwon</name><affiliation wicri:level="1"><nlm:affiliation>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Park, Hye Lim" sort="Park, Hye Lim" uniqKey="Park H" first="Hye-Lim" last="Park">Hye-Lim Park</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hun" sort="Kim, Hun" uniqKey="Kim H" first="Hun" last="Kim">Hun Kim</name><affiliation wicri:level="1"><nlm:affiliation>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sang Myeong" sort="Lee, Sang Myeong" uniqKey="Lee S" first="Sang-Myeong" last="Lee">Sang-Myeong Lee</name><affiliation wicri:level="1"><nlm:affiliation>Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea. Electronic address: leesangm@jbnu.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea. Electronic address: jhnam@catholic.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29739720</idno><idno type="pmid">29739720</idno><idno type="doi">10.1016/j.vaccine.2018.04.082</idno><idno type="wicri:Area/PubMed/Corpus">000910</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000910</idno><idno type="wicri:Area/PubMed/Curation">000910</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000910</idno><idno type="wicri:Area/PubMed/Checkpoint">000902</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000902</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Heterologous prime-boost vaccination with adenoviral vector and protein nanoparticles induces both Th1 and Th2 responses against Middle East respiratory syndrome coronavirus.</title><author><name sortKey="Jung, Seo Yeon" sort="Jung, Seo Yeon" uniqKey="Jung S" first="Seo-Yeon" last="Jung">Seo-Yeon Jung</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Kang, Kyung Won" sort="Kang, Kyung Won" uniqKey="Kang K" first="Kyung Won" last="Kang">Kyung Won Kang</name><affiliation wicri:level="1"><nlm:affiliation>Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Eun Young" sort="Lee, Eun Young" uniqKey="Lee E" first="Eun-Young" last="Lee">Eun-Young Lee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Seo, Dong Won" sort="Seo, Dong Won" uniqKey="Seo D" first="Dong-Won" last="Seo">Dong-Won Seo</name><affiliation wicri:level="1"><nlm:affiliation>Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hong Lim" sort="Kim, Hong Lim" uniqKey="Kim H" first="Hong-Lim" last="Kim">Hong-Lim Kim</name><affiliation wicri:level="1"><nlm:affiliation>Seoul St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Seoul St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Seoul 06591</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Hak" sort="Kim, Hak" uniqKey="Kim H" first="Hak" last="Kim">Hak Kim</name><affiliation wicri:level="1"><nlm:affiliation>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Kwon, Taewoo" sort="Kwon, Taewoo" uniqKey="Kwon T" first="Taewoo" last="Kwon">Taewoo Kwon</name><affiliation wicri:level="1"><nlm:affiliation>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Park, Hye Lim" sort="Park, Hye Lim" uniqKey="Park H" first="Hye-Lim" last="Park">Hye-Lim Park</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hun" sort="Kim, Hun" uniqKey="Kim H" first="Hun" last="Kim">Hun Kim</name><affiliation wicri:level="1"><nlm:affiliation>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of VAX R&D, Life Science Research Institute, SK Chemical, Seongnam 12771</wicri:regionArea><wicri:noRegion>Seongnam 12771</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sang Myeong" sort="Lee, Sang Myeong" uniqKey="Lee S" first="Sang-Myeong" last="Lee">Sang-Myeong Lee</name><affiliation wicri:level="1"><nlm:affiliation>Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea. Electronic address: leesangm@jbnu.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea><wicri:noRegion>Iksan 570-752</wicri:noRegion></affiliation></author><author><name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea. Electronic address: jhnam@catholic.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, The Catholic University of Korea, Bucheon 14662</wicri:regionArea><wicri:noRegion>Bucheon 14662</wicri:noRegion></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="eISSN">1873-2518</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviruses, Human (genetics)</term><term>Adenoviruses, Human (immunology)</term><term>Adjuvants, Immunologic (administration & dosage)</term><term>Alum Compounds (administration & dosage)</term><term>Animals</term><term>Antibodies, Neutralizing (biosynthesis)</term><term>Antibodies, Viral (biosynthesis)</term><term>Coronavirus Infections (immunology)</term><term>Coronavirus Infections (prevention & control)</term><term>Coronavirus Infections (virology)</term><term>Female</term><term>Humans</term><term>Immunization Schedule</term><term>Immunization, Secondary (methods)</term><term>Immunogenicity, Vaccine</term><term>Immunoglobulin G (biosynthesis)</term><term>Lymphocyte Activation (drug effects)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Middle East Respiratory Syndrome Coronavirus (drug effects)</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Nanoparticles (administration & dosage)</term><term>Nanoparticles (chemistry)</term><term>Spike Glycoprotein, Coronavirus (administration & dosage)</term><term>Spike Glycoprotein, Coronavirus (genetics)</term><term>Spike Glycoprotein, Coronavirus (immunology)</term><term>Th1 Cells (drug effects)</term><term>Th1 Cells (immunology)</term><term>Th1 Cells (virology)</term><term>Th2 Cells (drug effects)</term><term>Th2 Cells (immunology)</term><term>Th2 Cells (virology)</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (genetics)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes ()</term><term>Adjuvants immunologiques (administration et posologie)</term><term>Adénovirus humains (génétique)</term><term>Adénovirus humains (immunologie)</term><term>Alun (administration et posologie)</term><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Anticorps neutralisants (biosynthèse)</term><term>Calendrier vaccinal</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus (administration et posologie)</term><term>Glycoprotéine de spicule des coronavirus (génétique)</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Humains</term><term>Immunoglobuline G (biosynthèse)</term><term>Immunogénicité des vaccins</term><term>Infections à coronavirus ()</term><term>Infections à coronavirus (immunologie)</term><term>Infections à coronavirus (virologie)</term><term>Lymphocytes auxiliaires Th1 ()</term><term>Lymphocytes auxiliaires Th1 (immunologie)</term><term>Lymphocytes auxiliaires Th1 (virologie)</term><term>Lymphocytes auxiliaires Th2 ()</term><term>Lymphocytes auxiliaires Th2 (immunologie)</term><term>Lymphocytes auxiliaires Th2 (virologie)</term><term>Nanoparticules ()</term><term>Nanoparticules (administration et posologie)</term><term>Rappel de vaccin ()</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Vaccins antiviraux (administration et posologie)</term><term>Vaccins antiviraux (génétique)</term><term>Vaccins antiviraux (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Alum Compounds</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="administration & dosage" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Adjuvants immunologiques</term><term>Alun</term><term>Glycoprotéine de spicule des coronavirus</term><term>Nanoparticules</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lymphocyte Activation</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Th1 Cells</term><term>Th2 Cells</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviruses, Human</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adénovirus humains</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Adénovirus humains</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Infections à coronavirus</term><term>Lymphocytes auxiliaires Th1</term><term>Lymphocytes auxiliaires Th2</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Adenoviruses, Human</term><term>Coronavirus Infections</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Spike Glycoprotein, Coronavirus</term><term>Th1 Cells</term><term>Th2 Cells</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunization, Secondary</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Lymphocytes auxiliaires Th1</term><term>Lymphocytes auxiliaires Th2</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Th1 Cells</term><term>Th2 Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Humans</term><term>Immunization Schedule</term><term>Immunogenicity, Vaccine</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Calendrier vaccinal</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Femelle</term><term>Humains</term><term>Immunogénicité des vaccins</term><term>Infections à coronavirus</term><term>Lymphocytes auxiliaires Th1</term><term>Lymphocytes auxiliaires Th2</term><term>Nanoparticules</term><term>Rappel de vaccin</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic and zoonotic virus with a fatality rate in humans of over 35%. Although several vaccine candidates have been developed, there is still no clinically available vaccine for MERS-CoV. In this study, we developed two types of MERS-CoV vaccines: a recombinant adenovirus serotype 5 encoding the MERS-CoV spike gene (Ad5/MERS) and spike protein nanoparticles formulated with aluminum (alum) adjuvant. Next, we tested a heterologous prime-boost vaccine strategy, which compared priming with Ad5/MERS and boosting with spike protein nanoparticles and vice versa, with homologous prime-boost vaccination comprising priming and boosting with either spike protein nanoparticles or Ad5/MERS. Although both types of vaccine could induce specific immunoglobulin G against MERS-CoV, neutralizing antibodies against MERS-CoV were induced only by heterologous prime-boost immunization and homologous immunization with spike protein nanoparticles. Interestingly, Th1 cell activation was induced by immunization schedules including Ad5/MERS, but not by those including only spike protein nanoparticles. Heterologous prime-boost vaccination regimens including Ad5/MERS elicited simultaneous Th1 and Th2 responses, but homologous prime-boost regimens did not. Thus, heterologous prime-boost may induce longer-lasting immune responses against MERS-CoV because of an appropriate balance of Th1/Th2 responses. However, both heterologous prime-boost and homologous spike protein nanoparticles vaccinations could provide protection from MERS-CoV challenge in mice. Our results demonstrate that heterologous immunization by priming with Ad5/MERS and boosting with spike protein nanoparticles could be an efficient prophylactic strategy against MERS-CoV infection.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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