Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping.
Identifieur interne : 001952 ( Ncbi/Merge ); précédent : 001951; suivant : 001953Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping.
Auteurs : Yu Ishibashi [Japon] ; Shusuke Oura [Japon] ; Kazuo Umemura [Japon]Source :
- European biophysics journal : EBJ [ 1432-1017 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, DNA-Binding Proteins, Nanotubes, Carbon, Polyethylene Glycols, Rec A Recombinases.
- Adsorption, Models, Molecular, Molecular Conformation, Surface Properties.
Abstract
We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH2 groups (CONH2-SWNT) exhibited very strong interactions between the CONH2-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.
DOI: 10.1007/s00249-017-1200-3
PubMed: 28204854
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sortKey="Ishibashi, Yu" sort="Ishibashi, Yu" uniqKey="Ishibashi Y" first="Yu" last="Ishibashi">Yu Ishibashi</name><affiliation wicri:level="1"><nlm:affiliation>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601</wicri:regionArea><wicri:noRegion>1628601</wicri:noRegion></affiliation></author><author><name sortKey="Oura, Shusuke" sort="Oura, Shusuke" uniqKey="Oura S" first="Shusuke" last="Oura">Shusuke Oura</name><affiliation wicri:level="1"><nlm:affiliation>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601</wicri:regionArea><wicri:noRegion>1628601</wicri:noRegion></affiliation></author><author><name sortKey="Umemura, Kazuo" sort="Umemura, Kazuo" uniqKey="Umemura K" first="Kazuo" last="Umemura">Kazuo Umemura</name><affiliation wicri:level="1"><nlm:affiliation>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan. meicun2006@163.com.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601</wicri:regionArea><wicri:noRegion>1628601</wicri:noRegion></affiliation></author></analytic><series><title level="j">European biophysics journal : EBJ</title><idno type="eISSN">1432-1017</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adsorption</term><term>DNA (chemistry)</term><term>DNA-Binding Proteins (chemistry)</term><term>Models, Molecular</term><term>Molecular Conformation</term><term>Nanotubes, Carbon (chemistry)</term><term>Polyethylene Glycols (chemistry)</term><term>Rec A Recombinases (chemistry)</term><term>Surface Properties</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term><term>Adsorption</term><term>Conformation moléculaire</term><term>Modèles moléculaires</term><term>Nanotubes de carbone ()</term><term>Polyéthylène glycols ()</term><term>Propriétés de surface</term><term>Protéines de liaison à l'ADN ()</term><term>Rec A Recombinases ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA</term><term>DNA-Binding Proteins</term><term>Nanotubes, Carbon</term><term>Polyethylene Glycols</term><term>Rec A Recombinases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adsorption</term><term>Models, Molecular</term><term>Molecular Conformation</term><term>Surface Properties</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>Adsorption</term><term>Conformation moléculaire</term><term>Modèles moléculaires</term><term>Nanotubes de carbone</term><term>Polyéthylène glycols</term><term>Propriétés de surface</term><term>Protéines de liaison à l'ADN</term><term>Rec A Recombinases</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH<sub>2</sub> groups (CONH<sub>2</sub>-SWNT) exhibited very strong interactions between the CONH<sub>2</sub>-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28204854</PMID><DateCompleted><Year>2017</Year><Month>09</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1017</ISSN><JournalIssue CitedMedium="Internet"><Volume>46</Volume><Issue>6</Issue><PubDate><Year>2017</Year><Month>Sep</Month></PubDate></JournalIssue><Title>European biophysics journal : EBJ</Title><ISOAbbreviation>Eur. Biophys. J.</ISOAbbreviation></Journal><ArticleTitle>Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping.</ArticleTitle><Pagination><MedlinePgn>541-547</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00249-017-1200-3</ELocationID><Abstract><AbstractText>We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH<sub>2</sub> groups (CONH<sub>2</sub>-SWNT) exhibited very strong interactions between the CONH<sub>2</sub>-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ishibashi</LastName><ForeName>Yu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oura</LastName><ForeName>Shusuke</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Umemura</LastName><ForeName>Kazuo</ForeName><Initials>K</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-8073-3903</Identifier><AffiliationInfo><Affiliation>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan. meicun2006@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>02</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Eur Biophys J</MedlineTA><NlmUniqueID>8409413</NlmUniqueID><ISSNLinking>0175-7571</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037742">Nanotubes, Carbon</NameOfSubstance></Chemical><Chemical><RegistryNumber>3WJQ0SDW1A</RegistryNumber><NameOfSubstance UI="D011092">Polyethylene Glycols</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.-</RegistryNumber><NameOfSubstance UI="D011938">Rec A Recombinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000327" MajorTopicYN="N">Adsorption</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004268" MajorTopicYN="N">DNA-Binding Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, 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name="Japon"><noRegion><name sortKey="Ishibashi, Yu" sort="Ishibashi, Yu" uniqKey="Ishibashi Y" first="Yu" last="Ishibashi">Yu Ishibashi</name></noRegion><name sortKey="Oura, Shusuke" sort="Oura, Shusuke" uniqKey="Oura S" first="Shusuke" last="Oura">Shusuke Oura</name><name sortKey="Umemura, Kazuo" sort="Umemura, Kazuo" uniqKey="Umemura K" first="Kazuo" last="Umemura">Kazuo Umemura</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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