Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping.
Identifieur interne : 001952 ( Ncbi/Merge ); précédent : 001951; suivant : 001953Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping.
Auteurs : Yu Ishibashi [Japon] ; Shusuke Oura [Japon] ; Kazuo Umemura [Japon]Source :
- European biophysics journal : EBJ [ 1432-1017 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, DNA-Binding Proteins, Nanotubes, Carbon, Polyethylene Glycols, Rec A Recombinases.
- Adsorption, Models, Molecular, Molecular Conformation, Surface Properties.
Abstract
We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH2 groups (CONH2-SWNT) exhibited very strong interactions between the CONH2-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.
DOI: 10.1007/s00249-017-1200-3
PubMed: 28204854
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pubmed:28204854Le document en format XML
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<term>Molecular Conformation</term>
<term>Nanotubes, Carbon (chemistry)</term>
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<term>Rec A Recombinases</term>
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<front><div type="abstract" xml:lang="en">We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH<sub>2</sub>
groups (CONH<sub>2</sub>
-SWNT) exhibited very strong interactions between the CONH<sub>2</sub>
-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.</div>
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groups (CONH<sub>2</sub>
-SWNT) exhibited very strong interactions between the CONH<sub>2</sub>
-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.</AbstractText>
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<ReferenceList><Reference><Citation>Colloids Surf B Biointerfaces. 2013 Jun 1;106:234-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23434717</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Am Chem Soc. 2001 Apr 25;123(16):3838-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11457124</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biosens Bioelectron. 2015 Sep 15;71:1-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25879891</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Environ Sci Technol. 2008 Dec 15;42(24):9005-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19174865</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Am Chem Soc. 2005 Jun 8;127(22):8197-203</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15926849</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nano Lett. 2006 Feb;6(2):159-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16464027</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nanomaterials (Basel). 2016 Oct 08;6(10 ):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28335308</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Mater. 2003 May;2(5):338-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12692536</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Nanosci Nanotechnol. 2002 Oct;2(5):457-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12908278</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Colloids Surf B Biointerfaces. 2015 Feb 1;126:496-501</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25612818</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Colloids Surf B Biointerfaces. 2014 Sep 1;121:325-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24974776</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Drug Discov. 2003 Jan;2(1):29-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12509757</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Am Chem Soc. 2006 Jun 14;128(23):7492-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16756303</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Chem. 2008 Oct 1;80(19):7408-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18771233</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2002 Dec 19-26;420(6917):761</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12490938</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Colloids Surf B Biointerfaces. 2013 Jan 1;101:49-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22796771</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur Biophys J. 2016 Sep;45(6):483-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26846296</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nanoscale. 2011 Mar;3(3):967-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21229161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2011 Mar;39(6):e37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21183465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Commun (Camb). 2011 Mar 7;47(9):2625-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21234489</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nanomaterials (Basel). 2015 Mar 12;5(1):321-350</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28347014</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Soc Rev. 2009 Feb;38(2):329-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19169451</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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</country>
</list>
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