MersV1, Ncbi, Merge, bibRecord, 000281

Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.

Identifieur interne : 000281 ( Ncbi/Merge ); précédent : 000280; suivant : 000282

Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.

Auteurs : H. Stahlberg [Suisse] ; E. Kutejová ; K. Muchová ; M. Gregorini ; A. Lustig ; S A Müller ; V. Olivieri ; A. Engel ; A J Wilkinson ; I. Barák

Source :

Molecular microbiology [ 0950-382X ] ; 2004.

RBID : pubmed:15165232

Descripteurs français

English descriptors

KwdEn :
- Bacillus subtilis (chemistry), Bacterial Proteins (chemistry), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Bacterial Proteins (ultrastructure), Cell Cycle Proteins (chemistry), Cell Cycle Proteins (genetics), Cell Cycle Proteins (metabolism), Cell Cycle Proteins (ultrastructure), Microscopy, Electron, Models, Molecular, Protein Structure, Quaternary, Ultracentrifugation.
MESH :
- chemical , chemistry : Bacterial Proteins, Cell Cycle Proteins.
- chemistry : Bacillus subtilis.
- chemical , genetics : Bacterial Proteins, Cell Cycle Proteins.
- chemical , metabolism : Bacterial Proteins, Cell Cycle Proteins.
- chemical , ultrastructure : Bacterial Proteins, Cell Cycle Proteins.
- Microscopy, Electron, Models, Molecular, Protein Structure, Quaternary, Ultracentrifugation.

Abstract

DivIVA from Bacillus subtilis is a bifunctional protein with distinct roles in cell division and sporulation. During vegetative growth, DivIVA regulates the activity of the MinCD complex, thus helping to direct cell division to the correct mid-cell position. DivIVA fulfils a quite different role during sporulation in B. subtilis when it directs the oriC region of the chromosome to the cell pole before asymmetric cell division. DivIVA is a 19.5 kDa protein with a large part of its structure predicted to form a tropomyosin-like alpha-helical coiled-coil. Here, we present a model for the quaternary structure of DivIVA, based on cryonegative stain transmission electron microscopy images. The purified protein appears as an elongated particle with lateral expansions at both ends producing a form that resembles a 'doggy-bone'. The particle mass estimated from these images agrees with the value of 145 kDa measured by analytical ultracentrifugation suggesting 6- to 8-mers. These DivIVA oligomers serve as building blocks in the formation of higher order assemblies giving rise to strings, wires and, finally, two-dimensional lattices in a time-dependent manner.

DOI: 10.1111/j.1365-2958.2004.04074.x
PubMed: 15165232

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Le document en format XML

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<term>Bacterial Proteins (ultrastructure)</term>
<term>Cell Cycle Proteins (chemistry)</term>
<term>Cell Cycle Proteins (genetics)</term>
<term>Cell Cycle Proteins (metabolism)</term>
<term>Cell Cycle Proteins (ultrastructure)</term>
<term>Microscopy, Electron</term>
<term>Models, Molecular</term>
<term>Protein Structure, Quaternary</term>
<term>Ultracentrifugation</term>
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<term>Protéines bactériennes (génétique)</term>
<term>Protéines bactériennes (métabolisme)</term>
<term>Protéines bactériennes (ultrastructure)</term>
<term>Protéines du cycle cellulaire ()</term>
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<term>Protéines du cycle cellulaire (métabolisme)</term>
<term>Protéines du cycle cellulaire (ultrastructure)</term>
<term>Structure quaternaire des protéines</term>
<term>Ultracentrifugation</term>
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<front><div type="abstract" xml:lang="en">DivIVA from Bacillus subtilis is a bifunctional protein with distinct roles in cell division and sporulation. During vegetative growth, DivIVA regulates the activity of the MinCD complex, thus helping to direct cell division to the correct mid-cell position. DivIVA fulfils a quite different role during sporulation in B. subtilis when it directs the oriC region of the chromosome to the cell pole before asymmetric cell division. DivIVA is a 19.5 kDa protein with a large part of its structure predicted to form a tropomyosin-like alpha-helical coiled-coil. Here, we present a model for the quaternary structure of DivIVA, based on cryonegative stain transmission electron microscopy images. The purified protein appears as an elongated particle with lateral expansions at both ends producing a form that resembles a 'doggy-bone'. The particle mass estimated from these images agrees with the value of 145 kDa measured by analytical ultracentrifugation suggesting 6- to 8-mers. These DivIVA oligomers serve as building blocks in the formation of higher order assemblies giving rise to strings, wires and, finally, two-dimensional lattices in a time-dependent manner.</div>
</front>
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<Abstract><AbstractText>DivIVA from Bacillus subtilis is a bifunctional protein with distinct roles in cell division and sporulation. During vegetative growth, DivIVA regulates the activity of the MinCD complex, thus helping to direct cell division to the correct mid-cell position. DivIVA fulfils a quite different role during sporulation in B. subtilis when it directs the oriC region of the chromosome to the cell pole before asymmetric cell division. DivIVA is a 19.5 kDa protein with a large part of its structure predicted to form a tropomyosin-like alpha-helical coiled-coil. Here, we present a model for the quaternary structure of DivIVA, based on cryonegative stain transmission electron microscopy images. The purified protein appears as an elongated particle with lateral expansions at both ends producing a form that resembles a 'doggy-bone'. The particle mass estimated from these images agrees with the value of 145 kDa measured by analytical ultracentrifugation suggesting 6- to 8-mers. These DivIVA oligomers serve as building blocks in the formation of higher order assemblies giving rise to strings, wires and, finally, two-dimensional lattices in a time-dependent manner.</AbstractText>
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Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.

Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.

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Descripteurs français

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Abstract

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Links to Exploration step

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