Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.
Identifieur interne : 002247 ( PubMed/Checkpoint ); précédent : 002246; suivant : 002248Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.
Auteurs : H. Stahlberg [Suisse] ; E. Kutejová ; K. Muchová ; M. Gregorini ; A. Lustig ; S A Müller ; V. Olivieri ; A. Engel ; A J Wilkinson ; I. BarákSource :
- Molecular microbiology [ 0950-382X ] ; 2004.
Descripteurs français
- KwdFr :
- Bacillus subtilis (), Microscopie électronique, Modèles moléculaires, Protéines bactériennes (), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines bactériennes (ultrastructure), Protéines du cycle cellulaire (), Protéines du cycle cellulaire (génétique), Protéines du cycle cellulaire (métabolisme), Protéines du cycle cellulaire (ultrastructure), Structure quaternaire des protéines, Ultracentrifugation.
- MESH :
- génétique : Protéines bactériennes, Protéines du cycle cellulaire.
- métabolisme : Protéines bactériennes, Protéines du cycle cellulaire.
- Bacillus subtilis, Microscopie électronique, Modèles moléculaires, Protéines bactériennes, Protéines du cycle cellulaire, Structure quaternaire des protéines, Ultracentrifugation.
English descriptors
- KwdEn :
- Bacillus subtilis (chemistry), Bacterial Proteins (chemistry), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Bacterial Proteins (ultrastructure), Cell Cycle Proteins (chemistry), Cell Cycle Proteins (genetics), Cell Cycle Proteins (metabolism), Cell Cycle Proteins (ultrastructure), Microscopy, Electron, Models, Molecular, Protein Structure, Quaternary, Ultracentrifugation.
- MESH :
- chemical , chemistry : Bacterial Proteins, Cell Cycle Proteins.
- chemistry : Bacillus subtilis.
- chemical , genetics : Bacterial Proteins, Cell Cycle Proteins.
- chemical , metabolism : Bacterial Proteins, Cell Cycle Proteins.
- chemical , ultrastructure : Bacterial Proteins, Cell Cycle Proteins.
- Microscopy, Electron, Models, Molecular, Protein Structure, Quaternary, Ultracentrifugation.
Abstract
DivIVA from Bacillus subtilis is a bifunctional protein with distinct roles in cell division and sporulation. During vegetative growth, DivIVA regulates the activity of the MinCD complex, thus helping to direct cell division to the correct mid-cell position. DivIVA fulfils a quite different role during sporulation in B. subtilis when it directs the oriC region of the chromosome to the cell pole before asymmetric cell division. DivIVA is a 19.5 kDa protein with a large part of its structure predicted to form a tropomyosin-like alpha-helical coiled-coil. Here, we present a model for the quaternary structure of DivIVA, based on cryonegative stain transmission electron microscopy images. The purified protein appears as an elongated particle with lateral expansions at both ends producing a form that resembles a 'doggy-bone'. The particle mass estimated from these images agrees with the value of 145 kDa measured by analytical ultracentrifugation suggesting 6- to 8-mers. These DivIVA oligomers serve as building blocks in the formation of higher order assemblies giving rise to strings, wires and, finally, two-dimensional lattices in a time-dependent manner.
DOI: 10.1111/j.1365-2958.2004.04074.x
PubMed: 15165232
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:15165232Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.</title>
<author><name sortKey="Stahlberg, H" sort="Stahlberg, H" uniqKey="Stahlberg H" first="H" last="Stahlberg">H. Stahlberg</name>
<affiliation wicri:level="1"><nlm:affiliation>M. E. Müller Institute, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>M. E. Müller Institute, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel</wicri:regionArea>
<wicri:noRegion>CH-4056 Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kutejova, E" sort="Kutejova, E" uniqKey="Kutejova E" first="E" last="Kutejová">E. Kutejová</name>
</author>
<author><name sortKey="Muchova, K" sort="Muchova, K" uniqKey="Muchova K" first="K" last="Muchová">K. Muchová</name>
</author>
<author><name sortKey="Gregorini, M" sort="Gregorini, M" uniqKey="Gregorini M" first="M" last="Gregorini">M. Gregorini</name>
</author>
<author><name sortKey="Lustig, A" sort="Lustig, A" uniqKey="Lustig A" first="A" last="Lustig">A. Lustig</name>
</author>
<author><name sortKey="Muller, S A" sort="Muller, S A" uniqKey="Muller S" first="S A" last="Müller">S A Müller</name>
</author>
<author><name sortKey="Olivieri, V" sort="Olivieri, V" uniqKey="Olivieri V" first="V" last="Olivieri">V. Olivieri</name>
</author>
<author><name sortKey="Engel, A" sort="Engel, A" uniqKey="Engel A" first="A" last="Engel">A. Engel</name>
</author>
<author><name sortKey="Wilkinson, A J" sort="Wilkinson, A J" uniqKey="Wilkinson A" first="A J" last="Wilkinson">A J Wilkinson</name>
</author>
<author><name sortKey="Barak, I" sort="Barak, I" uniqKey="Barak I" first="I" last="Barák">I. Barák</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
<idno type="RBID">pubmed:15165232</idno>
<idno type="pmid">15165232</idno>
<idno type="doi">10.1111/j.1365-2958.2004.04074.x</idno>
<idno type="wicri:Area/PubMed/Corpus">002388</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002388</idno>
<idno type="wicri:Area/PubMed/Curation">002388</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002388</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002247</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002247</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.</title>
<author><name sortKey="Stahlberg, H" sort="Stahlberg, H" uniqKey="Stahlberg H" first="H" last="Stahlberg">H. Stahlberg</name>
<affiliation wicri:level="1"><nlm:affiliation>M. E. Müller Institute, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>M. E. Müller Institute, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel</wicri:regionArea>
<wicri:noRegion>CH-4056 Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kutejova, E" sort="Kutejova, E" uniqKey="Kutejova E" first="E" last="Kutejová">E. Kutejová</name>
</author>
<author><name sortKey="Muchova, K" sort="Muchova, K" uniqKey="Muchova K" first="K" last="Muchová">K. Muchová</name>
</author>
<author><name sortKey="Gregorini, M" sort="Gregorini, M" uniqKey="Gregorini M" first="M" last="Gregorini">M. Gregorini</name>
</author>
<author><name sortKey="Lustig, A" sort="Lustig, A" uniqKey="Lustig A" first="A" last="Lustig">A. Lustig</name>
</author>
<author><name sortKey="Muller, S A" sort="Muller, S A" uniqKey="Muller S" first="S A" last="Müller">S A Müller</name>
</author>
<author><name sortKey="Olivieri, V" sort="Olivieri, V" uniqKey="Olivieri V" first="V" last="Olivieri">V. Olivieri</name>
</author>
<author><name sortKey="Engel, A" sort="Engel, A" uniqKey="Engel A" first="A" last="Engel">A. Engel</name>
</author>
<author><name sortKey="Wilkinson, A J" sort="Wilkinson, A J" uniqKey="Wilkinson A" first="A J" last="Wilkinson">A J Wilkinson</name>
</author>
<author><name sortKey="Barak, I" sort="Barak, I" uniqKey="Barak I" first="I" last="Barák">I. Barák</name>
</author>
</analytic>
<series><title level="j">Molecular microbiology</title>
<idno type="ISSN">0950-382X</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacillus subtilis (chemistry)</term>
<term>Bacterial Proteins (chemistry)</term>
<term>Bacterial Proteins (genetics)</term>
<term>Bacterial Proteins (metabolism)</term>
<term>Bacterial Proteins (ultrastructure)</term>
<term>Cell Cycle Proteins (chemistry)</term>
<term>Cell Cycle Proteins (genetics)</term>
<term>Cell Cycle Proteins (metabolism)</term>
<term>Cell Cycle Proteins (ultrastructure)</term>
<term>Microscopy, Electron</term>
<term>Models, Molecular</term>
<term>Protein Structure, Quaternary</term>
<term>Ultracentrifugation</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Bacillus subtilis ()</term>
<term>Microscopie électronique</term>
<term>Modèles moléculaires</term>
<term>Protéines bactériennes ()</term>
<term>Protéines bactériennes (génétique)</term>
<term>Protéines bactériennes (métabolisme)</term>
<term>Protéines bactériennes (ultrastructure)</term>
<term>Protéines du cycle cellulaire ()</term>
<term>Protéines du cycle cellulaire (génétique)</term>
<term>Protéines du cycle cellulaire (métabolisme)</term>
<term>Protéines du cycle cellulaire (ultrastructure)</term>
<term>Structure quaternaire des protéines</term>
<term>Ultracentrifugation</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Bacterial Proteins</term>
<term>Cell Cycle Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Bacillus subtilis</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term>
<term>Cell Cycle Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines bactériennes</term>
<term>Protéines du cycle cellulaire</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term>
<term>Cell Cycle Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines bactériennes</term>
<term>Protéines du cycle cellulaire</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Bacterial Proteins</term>
<term>Cell Cycle Proteins</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Microscopy, Electron</term>
<term>Models, Molecular</term>
<term>Protein Structure, Quaternary</term>
<term>Ultracentrifugation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Bacillus subtilis</term>
<term>Microscopie électronique</term>
<term>Modèles moléculaires</term>
<term>Protéines bactériennes</term>
<term>Protéines du cycle cellulaire</term>
<term>Structure quaternaire des protéines</term>
<term>Ultracentrifugation</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">DivIVA from Bacillus subtilis is a bifunctional protein with distinct roles in cell division and sporulation. During vegetative growth, DivIVA regulates the activity of the MinCD complex, thus helping to direct cell division to the correct mid-cell position. DivIVA fulfils a quite different role during sporulation in B. subtilis when it directs the oriC region of the chromosome to the cell pole before asymmetric cell division. DivIVA is a 19.5 kDa protein with a large part of its structure predicted to form a tropomyosin-like alpha-helical coiled-coil. Here, we present a model for the quaternary structure of DivIVA, based on cryonegative stain transmission electron microscopy images. The purified protein appears as an elongated particle with lateral expansions at both ends producing a form that resembles a 'doggy-bone'. The particle mass estimated from these images agrees with the value of 145 kDa measured by analytical ultracentrifugation suggesting 6- to 8-mers. These DivIVA oligomers serve as building blocks in the formation of higher order assemblies giving rise to strings, wires and, finally, two-dimensional lattices in a time-dependent manner.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15165232</PMID>
<DateCompleted><Year>2004</Year>
<Month>08</Month>
<Day>06</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0950-382X</ISSN>
<JournalIssue CitedMedium="Print"><Volume>52</Volume>
<Issue>5</Issue>
<PubDate><Year>2004</Year>
<Month>Jun</Month>
</PubDate>
</JournalIssue>
<Title>Molecular microbiology</Title>
<ISOAbbreviation>Mol. Microbiol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.</ArticleTitle>
<Pagination><MedlinePgn>1281-90</MedlinePgn>
</Pagination>
<Abstract><AbstractText>DivIVA from Bacillus subtilis is a bifunctional protein with distinct roles in cell division and sporulation. During vegetative growth, DivIVA regulates the activity of the MinCD complex, thus helping to direct cell division to the correct mid-cell position. DivIVA fulfils a quite different role during sporulation in B. subtilis when it directs the oriC region of the chromosome to the cell pole before asymmetric cell division. DivIVA is a 19.5 kDa protein with a large part of its structure predicted to form a tropomyosin-like alpha-helical coiled-coil. Here, we present a model for the quaternary structure of DivIVA, based on cryonegative stain transmission electron microscopy images. The purified protein appears as an elongated particle with lateral expansions at both ends producing a form that resembles a 'doggy-bone'. The particle mass estimated from these images agrees with the value of 145 kDa measured by analytical ultracentrifugation suggesting 6- to 8-mers. These DivIVA oligomers serve as building blocks in the formation of higher order assemblies giving rise to strings, wires and, finally, two-dimensional lattices in a time-dependent manner.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Stahlberg</LastName>
<ForeName>H</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>M. E. Müller Institute, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kutejová</LastName>
<ForeName>E</ForeName>
<Initials>E</Initials>
</Author>
<Author ValidYN="Y"><LastName>Muchová</LastName>
<ForeName>K</ForeName>
<Initials>K</Initials>
</Author>
<Author ValidYN="Y"><LastName>Gregorini</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lustig</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Müller</LastName>
<ForeName>S A</ForeName>
<Initials>SA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Olivieri</LastName>
<ForeName>V</ForeName>
<Initials>V</Initials>
</Author>
<Author ValidYN="Y"><LastName>Engel</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wilkinson</LastName>
<ForeName>A J</ForeName>
<Initials>AJ</Initials>
</Author>
<Author ValidYN="Y"><LastName>Barák</LastName>
<ForeName>I</ForeName>
<Initials>I</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Mol Microbiol</MedlineTA>
<NlmUniqueID>8712028</NlmUniqueID>
<ISSNLinking>0950-382X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018797">Cell Cycle Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C104636">DivIVA protein, bacteria</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001412" MajorTopicYN="N">Bacillus subtilis</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018797" MajorTopicYN="N">Cell Cycle Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008854" MajorTopicYN="N">Microscopy, Electron</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020836" MajorTopicYN="Y">Protein Structure, Quaternary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014461" MajorTopicYN="N">Ultracentrifugation</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year>
<Month>5</Month>
<Day>29</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2004</Year>
<Month>8</Month>
<Day>7</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2004</Year>
<Month>5</Month>
<Day>29</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">15165232</ArticleId>
<ArticleId IdType="doi">10.1111/j.1365-2958.2004.04074.x</ArticleId>
<ArticleId IdType="pii">MMI4074</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Suisse</li>
</country>
</list>
<tree><noCountry><name sortKey="Barak, I" sort="Barak, I" uniqKey="Barak I" first="I" last="Barák">I. Barák</name>
<name sortKey="Engel, A" sort="Engel, A" uniqKey="Engel A" first="A" last="Engel">A. Engel</name>
<name sortKey="Gregorini, M" sort="Gregorini, M" uniqKey="Gregorini M" first="M" last="Gregorini">M. Gregorini</name>
<name sortKey="Kutejova, E" sort="Kutejova, E" uniqKey="Kutejova E" first="E" last="Kutejová">E. Kutejová</name>
<name sortKey="Lustig, A" sort="Lustig, A" uniqKey="Lustig A" first="A" last="Lustig">A. Lustig</name>
<name sortKey="Muchova, K" sort="Muchova, K" uniqKey="Muchova K" first="K" last="Muchová">K. Muchová</name>
<name sortKey="Muller, S A" sort="Muller, S A" uniqKey="Muller S" first="S A" last="Müller">S A Müller</name>
<name sortKey="Olivieri, V" sort="Olivieri, V" uniqKey="Olivieri V" first="V" last="Olivieri">V. Olivieri</name>
<name sortKey="Wilkinson, A J" sort="Wilkinson, A J" uniqKey="Wilkinson A" first="A J" last="Wilkinson">A J Wilkinson</name>
</noCountry>
<country name="Suisse"><noRegion><name sortKey="Stahlberg, H" sort="Stahlberg, H" uniqKey="Stahlberg H" first="H" last="Stahlberg">H. Stahlberg</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002247 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002247 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:15165232 |texte= Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:15165232" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |