Unmodified phosphodiester antisense oligodeoxynucleotides to the BCR-ABL junction do not suppress Philadelphia-positive clonogenic cells.
Identifieur interne : 002918 ( Ncbi/Curation ); précédent : 002917; suivant : 002919Unmodified phosphodiester antisense oligodeoxynucleotides to the BCR-ABL junction do not suppress Philadelphia-positive clonogenic cells.
Auteurs : A. K Bisch [Allemagne] ; L. Pérènyi ; U. Seay ; J. Lohmeyer ; H. PralleSource :
- Acta haematologica [ 0001-5792 ] ; 1994.
Descripteurs français
- KwdFr :
- ARN tumoral (génétique), Amorces ADN (), Cellules cancéreuses en culture, Clones cellulaires, Données de séquences moléculaires, Humains, Hématopoïèse (), Leucémie myéloïde chronique BCR-ABL positive (), Leucémie myéloïde chronique BCR-ABL positive (anatomopathologie), Oligonucléotides antisens (), Protéines de fusion bcr-abl (génétique), Séquence nucléotidique, Techniques in vitro.
- MESH :
- anatomopathologie : Leucémie myéloïde chronique BCR-ABL positive.
- génétique : ARN tumoral, Protéines de fusion bcr-abl.
- Amorces ADN, Cellules cancéreuses en culture, Clones cellulaires, Données de séquences moléculaires, Humains, Hématopoïèse, Leucémie myéloïde chronique BCR-ABL positive, Oligonucléotides antisens, Séquence nucléotidique, Techniques in vitro.
English descriptors
- KwdEn :
- Base Sequence, Clone Cells, DNA Primers (chemistry), Fusion Proteins, bcr-abl (genetics), Hematopoiesis (drug effects), Humans, In Vitro Techniques, Leukemia, Myelogenous, Chronic, BCR-ABL Positive (pathology), Leukemia, Myelogenous, Chronic, BCR-ABL Positive (therapy), Molecular Sequence Data, Oligonucleotides, Antisense (chemistry), RNA, Neoplasm (genetics), Tumor Cells, Cultured.
- MESH :
- chemical , chemistry : DNA Primers, Oligonucleotides, Antisense.
- chemical , genetics : Fusion Proteins, bcr-abl, RNA, Neoplasm.
- drug effects : Hematopoiesis.
- pathology : Leukemia, Myelogenous, Chronic, BCR-ABL Positive.
- therapy : Leukemia, Myelogenous, Chronic, BCR-ABL Positive.
- Base Sequence, Clone Cells, Humans, In Vitro Techniques, Molecular Sequence Data, Tumor Cells, Cultured.
Abstract
The BCR-ABL fusion gene is directly involved in the pathogenesis of chronic myeloid leukemia (CML). Specific inhibition of the BCR-ABL gene expression with antisense oligodeoxynucleotides has been shown to have profound effects on cell growth in vitro. We examined antisense phosphodiester oligonucleotides (16-mers at a concentration of 60 micrograms/ml) spanning the two possible junction sites K28 (b3a2) and L6 (b2a2) in a clonogenic assay. Single colonies from 9 patients with CML and from patients with normal bone marrow were screened for BCR-ABL expression with a new 'single-tube nested PCR' method. There was a marked reduction in colony number in the CML group and a lesser growth inhibition in the control group. The number and percentage of BCR-ABL-positive colonies, however, was not reduced in the CML group. This indicates a nonspecific growth inhibition only.
DOI: 10.1159/000204219
PubMed: 7701917
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pubmed:7701917Le document en format XML
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K Bisch</name><affiliation wicri:level="1"><nlm:affiliation>Department of Hematology and Oncology, Justus Liebig University, Giessen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Hematology and Oncology, Justus Liebig University, Giessen</wicri:regionArea><wicri:noRegion>Giessen</wicri:noRegion><wicri:noRegion>Giessen</wicri:noRegion><wicri:noRegion>Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Perenyi, L" sort="Perenyi, L" uniqKey="Perenyi L" first="L" last="Pérènyi">L. Pérènyi</name></author><author><name sortKey="Seay, U" sort="Seay, U" uniqKey="Seay U" first="U" last="Seay">U. Seay</name></author><author><name sortKey="Lohmeyer, J" sort="Lohmeyer, J" uniqKey="Lohmeyer J" first="J" last="Lohmeyer">J. Lohmeyer</name></author><author><name sortKey="Pralle, H" sort="Pralle, H" uniqKey="Pralle H" first="H" last="Pralle">H. Pralle</name></author></analytic><series><title level="j">Acta haematologica</title><idno type="ISSN">0001-5792</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Clone Cells</term><term>DNA Primers (chemistry)</term><term>Fusion Proteins, bcr-abl (genetics)</term><term>Hematopoiesis (drug effects)</term><term>Humans</term><term>In Vitro Techniques</term><term>Leukemia, Myelogenous, Chronic, BCR-ABL Positive (pathology)</term><term>Leukemia, Myelogenous, Chronic, BCR-ABL Positive (therapy)</term><term>Molecular Sequence Data</term><term>Oligonucleotides, Antisense (chemistry)</term><term>RNA, Neoplasm (genetics)</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN tumoral (génétique)</term><term>Amorces ADN ()</term><term>Cellules cancéreuses en culture</term><term>Clones cellulaires</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Hématopoïèse ()</term><term>Leucémie myéloïde chronique BCR-ABL positive ()</term><term>Leucémie myéloïde chronique BCR-ABL positive (anatomopathologie)</term><term>Oligonucléotides antisens ()</term><term>Protéines de fusion bcr-abl (génétique)</term><term>Séquence nucléotidique</term><term>Techniques in vitro</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA Primers</term><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fusion Proteins, bcr-abl</term><term>RNA, Neoplasm</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Leucémie myéloïde chronique BCR-ABL positive</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Hematopoiesis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN tumoral</term><term>Protéines de fusion bcr-abl</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Leukemia, Myelogenous, Chronic, BCR-ABL Positive</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Leukemia, Myelogenous, Chronic, BCR-ABL Positive</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Clone Cells</term><term>Humans</term><term>In Vitro Techniques</term><term>Molecular Sequence Data</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Cellules cancéreuses en culture</term><term>Clones cellulaires</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Hématopoïèse</term><term>Leucémie myéloïde chronique BCR-ABL positive</term><term>Oligonucléotides antisens</term><term>Séquence nucléotidique</term><term>Techniques in vitro</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The BCR-ABL fusion gene is directly involved in the pathogenesis of chronic myeloid leukemia (CML). Specific inhibition of the BCR-ABL gene expression with antisense oligodeoxynucleotides has been shown to have profound effects on cell growth in vitro. We examined antisense phosphodiester oligonucleotides (16-mers at a concentration of 60 micrograms/ml) spanning the two possible junction sites K28 (b3a2) and L6 (b2a2) in a clonogenic assay. Single colonies from 9 patients with CML and from patients with normal bone marrow were screened for BCR-ABL expression with a new 'single-tube nested PCR' method. There was a marked reduction in colony number in the CML group and a lesser growth inhibition in the control group. The number and percentage of BCR-ABL-positive colonies, however, was not reduced in the CML group. This indicates a nonspecific growth inhibition only.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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