Unmodified phosphodiester antisense oligodeoxynucleotides to the BCR-ABL junction do not suppress Philadelphia-positive clonogenic cells.
Identifieur interne : 002918 ( Ncbi/Curation ); précédent : 002917; suivant : 002919Unmodified phosphodiester antisense oligodeoxynucleotides to the BCR-ABL junction do not suppress Philadelphia-positive clonogenic cells.
Auteurs : A. K Bisch [Allemagne] ; L. Pérènyi ; U. Seay ; J. Lohmeyer ; H. PralleSource :
- Acta haematologica [ 0001-5792 ] ; 1994.
Descripteurs français
- KwdFr :
- ARN tumoral (génétique), Amorces ADN (), Cellules cancéreuses en culture, Clones cellulaires, Données de séquences moléculaires, Humains, Hématopoïèse (), Leucémie myéloïde chronique BCR-ABL positive (), Leucémie myéloïde chronique BCR-ABL positive (anatomopathologie), Oligonucléotides antisens (), Protéines de fusion bcr-abl (génétique), Séquence nucléotidique, Techniques in vitro.
- MESH :
- anatomopathologie : Leucémie myéloïde chronique BCR-ABL positive.
- génétique : ARN tumoral, Protéines de fusion bcr-abl.
- Amorces ADN, Cellules cancéreuses en culture, Clones cellulaires, Données de séquences moléculaires, Humains, Hématopoïèse, Leucémie myéloïde chronique BCR-ABL positive, Oligonucléotides antisens, Séquence nucléotidique, Techniques in vitro.
English descriptors
- KwdEn :
- Base Sequence, Clone Cells, DNA Primers (chemistry), Fusion Proteins, bcr-abl (genetics), Hematopoiesis (drug effects), Humans, In Vitro Techniques, Leukemia, Myelogenous, Chronic, BCR-ABL Positive (pathology), Leukemia, Myelogenous, Chronic, BCR-ABL Positive (therapy), Molecular Sequence Data, Oligonucleotides, Antisense (chemistry), RNA, Neoplasm (genetics), Tumor Cells, Cultured.
- MESH :
- chemical , chemistry : DNA Primers, Oligonucleotides, Antisense.
- chemical , genetics : Fusion Proteins, bcr-abl, RNA, Neoplasm.
- drug effects : Hematopoiesis.
- pathology : Leukemia, Myelogenous, Chronic, BCR-ABL Positive.
- therapy : Leukemia, Myelogenous, Chronic, BCR-ABL Positive.
- Base Sequence, Clone Cells, Humans, In Vitro Techniques, Molecular Sequence Data, Tumor Cells, Cultured.
Abstract
The BCR-ABL fusion gene is directly involved in the pathogenesis of chronic myeloid leukemia (CML). Specific inhibition of the BCR-ABL gene expression with antisense oligodeoxynucleotides has been shown to have profound effects on cell growth in vitro. We examined antisense phosphodiester oligonucleotides (16-mers at a concentration of 60 micrograms/ml) spanning the two possible junction sites K28 (b3a2) and L6 (b2a2) in a clonogenic assay. Single colonies from 9 patients with CML and from patients with normal bone marrow were screened for BCR-ABL expression with a new 'single-tube nested PCR' method. There was a marked reduction in colony number in the CML group and a lesser growth inhibition in the control group. The number and percentage of BCR-ABL-positive colonies, however, was not reduced in the CML group. This indicates a nonspecific growth inhibition only.
DOI: 10.1159/000204219
PubMed: 7701917
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pubmed:7701917Le document en format XML
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<term>DNA Primers (chemistry)</term>
<term>Fusion Proteins, bcr-abl (genetics)</term>
<term>Hematopoiesis (drug effects)</term>
<term>Humans</term>
<term>In Vitro Techniques</term>
<term>Leukemia, Myelogenous, Chronic, BCR-ABL Positive (pathology)</term>
<term>Leukemia, Myelogenous, Chronic, BCR-ABL Positive (therapy)</term>
<term>Molecular Sequence Data</term>
<term>Oligonucleotides, Antisense (chemistry)</term>
<term>RNA, Neoplasm (genetics)</term>
<term>Tumor Cells, Cultured</term>
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<term>Amorces ADN ()</term>
<term>Cellules cancéreuses en culture</term>
<term>Clones cellulaires</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Hématopoïèse ()</term>
<term>Leucémie myéloïde chronique BCR-ABL positive ()</term>
<term>Leucémie myéloïde chronique BCR-ABL positive (anatomopathologie)</term>
<term>Oligonucléotides antisens ()</term>
<term>Protéines de fusion bcr-abl (génétique)</term>
<term>Séquence nucléotidique</term>
<term>Techniques in vitro</term>
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<term>Oligonucleotides, Antisense</term>
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<term>RNA, Neoplasm</term>
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<term>Protéines de fusion bcr-abl</term>
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<term>Humans</term>
<term>In Vitro Techniques</term>
<term>Molecular Sequence Data</term>
<term>Tumor Cells, Cultured</term>
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<term>Cellules cancéreuses en culture</term>
<term>Clones cellulaires</term>
<term>Données de séquences moléculaires</term>
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<term>Hématopoïèse</term>
<term>Leucémie myéloïde chronique BCR-ABL positive</term>
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<front><div type="abstract" xml:lang="en">The BCR-ABL fusion gene is directly involved in the pathogenesis of chronic myeloid leukemia (CML). Specific inhibition of the BCR-ABL gene expression with antisense oligodeoxynucleotides has been shown to have profound effects on cell growth in vitro. We examined antisense phosphodiester oligonucleotides (16-mers at a concentration of 60 micrograms/ml) spanning the two possible junction sites K28 (b3a2) and L6 (b2a2) in a clonogenic assay. Single colonies from 9 patients with CML and from patients with normal bone marrow were screened for BCR-ABL expression with a new 'single-tube nested PCR' method. There was a marked reduction in colony number in the CML group and a lesser growth inhibition in the control group. The number and percentage of BCR-ABL-positive colonies, however, was not reduced in the CML group. This indicates a nonspecific growth inhibition only.</div>
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