MersV1, PubMed, Checkpoint, bibRecord, 002701

Unmodified phosphodiester antisense oligodeoxynucleotides to the BCR-ABL junction do not suppress Philadelphia-positive clonogenic cells.

Identifieur interne : 002701 ( PubMed/Checkpoint ); précédent : 002700; suivant : 002702

Unmodified phosphodiester antisense oligodeoxynucleotides to the BCR-ABL junction do not suppress Philadelphia-positive clonogenic cells.

Auteurs : A. K Bisch [Allemagne] ; L. Pérènyi ; U. Seay ; J. Lohmeyer ; H. Pralle

Source :

Acta haematologica [ 0001-5792 ] ; 1994.

RBID : pubmed:7701917

Descripteurs français

English descriptors

KwdEn :
- Base Sequence, Clone Cells, DNA Primers (chemistry), Fusion Proteins, bcr-abl (genetics), Hematopoiesis (drug effects), Humans, In Vitro Techniques, Leukemia, Myelogenous, Chronic, BCR-ABL Positive (pathology), Leukemia, Myelogenous, Chronic, BCR-ABL Positive (therapy), Molecular Sequence Data, Oligonucleotides, Antisense (chemistry), RNA, Neoplasm (genetics), Tumor Cells, Cultured.
MESH :
- chemical , chemistry : DNA Primers, Oligonucleotides, Antisense.
- chemical , genetics : Fusion Proteins, bcr-abl, RNA, Neoplasm.
- drug effects : Hematopoiesis.
- pathology : Leukemia, Myelogenous, Chronic, BCR-ABL Positive.
- therapy : Leukemia, Myelogenous, Chronic, BCR-ABL Positive.
- Base Sequence, Clone Cells, Humans, In Vitro Techniques, Molecular Sequence Data, Tumor Cells, Cultured.

Abstract

The BCR-ABL fusion gene is directly involved in the pathogenesis of chronic myeloid leukemia (CML). Specific inhibition of the BCR-ABL gene expression with antisense oligodeoxynucleotides has been shown to have profound effects on cell growth in vitro. We examined antisense phosphodiester oligonucleotides (16-mers at a concentration of 60 micrograms/ml) spanning the two possible junction sites K28 (b3a2) and L6 (b2a2) in a clonogenic assay. Single colonies from 9 patients with CML and from patients with normal bone marrow were screened for BCR-ABL expression with a new 'single-tube nested PCR' method. There was a marked reduction in colony number in the CML group and a lesser growth inhibition in the control group. The number and percentage of BCR-ABL-positive colonies, however, was not reduced in the CML group. This indicates a nonspecific growth inhibition only.

DOI: 10.1159/000204219
PubMed: 7701917

Affiliations:

Allemagne

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pubmed:7701917

Le document en format XML

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<affiliation wicri:level="1"><nlm:affiliation>Department of Hematology and Oncology, Justus Liebig University, Giessen, Germany.</nlm:affiliation>
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<term>Hematopoiesis (drug effects)</term>
<term>Humans</term>
<term>In Vitro Techniques</term>
<term>Leukemia, Myelogenous, Chronic, BCR-ABL Positive (pathology)</term>
<term>Leukemia, Myelogenous, Chronic, BCR-ABL Positive (therapy)</term>
<term>Molecular Sequence Data</term>
<term>Oligonucleotides, Antisense (chemistry)</term>
<term>RNA, Neoplasm (genetics)</term>
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<term>Clones cellulaires</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Hématopoïèse ()</term>
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<term>Leucémie myéloïde chronique BCR-ABL positive (anatomopathologie)</term>
<term>Oligonucléotides antisens ()</term>
<term>Protéines de fusion bcr-abl (génétique)</term>
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<term>Techniques in vitro</term>
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<term>Molecular Sequence Data</term>
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<term>Données de séquences moléculaires</term>
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<term>Hématopoïèse</term>
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<front><div type="abstract" xml:lang="en">The BCR-ABL fusion gene is directly involved in the pathogenesis of chronic myeloid leukemia (CML). Specific inhibition of the BCR-ABL gene expression with antisense oligodeoxynucleotides has been shown to have profound effects on cell growth in vitro. We examined antisense phosphodiester oligonucleotides (16-mers at a concentration of 60 micrograms/ml) spanning the two possible junction sites K28 (b3a2) and L6 (b2a2) in a clonogenic assay. Single colonies from 9 patients with CML and from patients with normal bone marrow were screened for BCR-ABL expression with a new 'single-tube nested PCR' method. There was a marked reduction in colony number in the CML group and a lesser growth inhibition in the control group. The number and percentage of BCR-ABL-positive colonies, however, was not reduced in the CML group. This indicates a nonspecific growth inhibition only.</div>
</front>
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</Pagination>
<Abstract><AbstractText>The BCR-ABL fusion gene is directly involved in the pathogenesis of chronic myeloid leukemia (CML). Specific inhibition of the BCR-ABL gene expression with antisense oligodeoxynucleotides has been shown to have profound effects on cell growth in vitro. We examined antisense phosphodiester oligonucleotides (16-mers at a concentration of 60 micrograms/ml) spanning the two possible junction sites K28 (b3a2) and L6 (b2a2) in a clonogenic assay. Single colonies from 9 patients with CML and from patients with normal bone marrow were screened for BCR-ABL expression with a new 'single-tube nested PCR' method. There was a marked reduction in colony number in the CML group and a lesser growth inhibition in the control group. The number and percentage of BCR-ABL-positive colonies, however, was not reduced in the CML group. This indicates a nonspecific growth inhibition only.</AbstractText>
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Unmodified phosphodiester antisense oligodeoxynucleotides to the BCR-ABL junction do not suppress Philadelphia-positive clonogenic cells.

Unmodified phosphodiester antisense oligodeoxynucleotides to the BCR-ABL junction do not suppress Philadelphia-positive clonogenic cells.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki