Ionic strength-dependent persistence lengths of single-stranded RNA and DNA.
Identifieur interne : 000913 ( Ncbi/Curation ); précédent : 000912; suivant : 000914Ionic strength-dependent persistence lengths of single-stranded RNA and DNA.
Auteurs : Huimin Chen [États-Unis] ; Steve P. Meisburger ; Suzette A. Pabit ; Julie L. Sutton ; Watt W. Webb ; Lois PollackSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, Single-Stranded, RNA.
- chemical , pharmacology : Magnesium Chloride, Sodium Chloride.
- drug effects : Pliability.
- Fluorescence Resonance Energy Transfer, Ions, Models, Molecular, Osmolar Concentration, Scattering, Small Angle, X-Ray Diffraction.
Abstract
Dynamic RNA molecules carry out essential processes in the cell including translation and splicing. Base-pair interactions stabilize RNA into relatively rigid structures, while flexible non-base-paired regions allow RNA to undergo conformational changes required for function. To advance our understanding of RNA folding and dynamics it is critical to know the flexibility of these un-base-paired regions and how it depends on counterions. Yet, information about nucleic acid polymer properties is mainly derived from studies of ssDNA. Here we measure the persistence lengths (l(p)) of ssRNA. We observe valence and ionic strength-dependent differences in l(p) in a direct comparison between 40-mers of deoxythymidylate (dT(40)) and uridylate (rU(40)) measured using the powerful combination of SAXS and smFRET. We also show that nucleic acid flexibility is influenced by local environment (an adjoining double helix). Our results illustrate the complex interplay between conformation and ion environment that modulates nucleic acid function in vivo.
DOI: 10.1073/pnas.1119057109
PubMed: 22203973
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pubmed:22203973Le document en format XML
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<term>Fluorescence Resonance Energy Transfer</term>
<term>Ions</term>
<term>Magnesium Chloride (pharmacology)</term>
<term>Models, Molecular</term>
<term>Osmolar Concentration</term>
<term>Pliability (drug effects)</term>
<term>RNA (chemistry)</term>
<term>Scattering, Small Angle</term>
<term>Sodium Chloride (pharmacology)</term>
<term>X-Ray Diffraction</term>
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<term>Concentration osmolaire</term>
<term>Diffraction des rayons X</term>
<term>Diffusion aux petits angles</term>
<term>Flexibilité ()</term>
<term>Ions</term>
<term>Modèles moléculaires</term>
<term>Transfert d'énergie par résonance de fluorescence</term>
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<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chlorure de magnésium</term>
<term>Chlorure de sodium</term>
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<term>Ions</term>
<term>Models, Molecular</term>
<term>Osmolar Concentration</term>
<term>Scattering, Small Angle</term>
<term>X-Ray Diffraction</term>
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<term>Concentration osmolaire</term>
<term>Diffraction des rayons X</term>
<term>Diffusion aux petits angles</term>
<term>Flexibilité</term>
<term>Ions</term>
<term>Modèles moléculaires</term>
<term>Transfert d'énergie par résonance de fluorescence</term>
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<front><div type="abstract" xml:lang="en">Dynamic RNA molecules carry out essential processes in the cell including translation and splicing. Base-pair interactions stabilize RNA into relatively rigid structures, while flexible non-base-paired regions allow RNA to undergo conformational changes required for function. To advance our understanding of RNA folding and dynamics it is critical to know the flexibility of these un-base-paired regions and how it depends on counterions. Yet, information about nucleic acid polymer properties is mainly derived from studies of ssDNA. Here we measure the persistence lengths (l(p)) of ssRNA. We observe valence and ionic strength-dependent differences in l(p) in a direct comparison between 40-mers of deoxythymidylate (dT(40)) and uridylate (rU(40)) measured using the powerful combination of SAXS and smFRET. We also show that nucleic acid flexibility is influenced by local environment (an adjoining double helix). Our results illustrate the complex interplay between conformation and ion environment that modulates nucleic acid function in vivo.</div>
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