Ionic strength-dependent persistence lengths of single-stranded RNA and DNA.
Identifieur interne : 000913 ( Ncbi/Merge ); précédent : 000912; suivant : 000914Ionic strength-dependent persistence lengths of single-stranded RNA and DNA.
Auteurs : Huimin Chen [États-Unis] ; Steve P. Meisburger ; Suzette A. Pabit ; Julie L. Sutton ; Watt W. Webb ; Lois PollackSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, Single-Stranded, RNA.
- chemical , pharmacology : Magnesium Chloride, Sodium Chloride.
- drug effects : Pliability.
- Fluorescence Resonance Energy Transfer, Ions, Models, Molecular, Osmolar Concentration, Scattering, Small Angle, X-Ray Diffraction.
Abstract
Dynamic RNA molecules carry out essential processes in the cell including translation and splicing. Base-pair interactions stabilize RNA into relatively rigid structures, while flexible non-base-paired regions allow RNA to undergo conformational changes required for function. To advance our understanding of RNA folding and dynamics it is critical to know the flexibility of these un-base-paired regions and how it depends on counterions. Yet, information about nucleic acid polymer properties is mainly derived from studies of ssDNA. Here we measure the persistence lengths (l(p)) of ssRNA. We observe valence and ionic strength-dependent differences in l(p) in a direct comparison between 40-mers of deoxythymidylate (dT(40)) and uridylate (rU(40)) measured using the powerful combination of SAXS and smFRET. We also show that nucleic acid flexibility is influenced by local environment (an adjoining double helix). Our results illustrate the complex interplay between conformation and ion environment that modulates nucleic acid function in vivo.
DOI: 10.1073/pnas.1119057109
PubMed: 22203973
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Meisburger</name></author><author><name sortKey="Pabit, Suzette A" sort="Pabit, Suzette A" uniqKey="Pabit S" first="Suzette A" last="Pabit">Suzette A. Pabit</name></author><author><name sortKey="Sutton, Julie L" sort="Sutton, Julie L" uniqKey="Sutton J" first="Julie L" last="Sutton">Julie L. Sutton</name></author><author><name sortKey="Webb, Watt W" sort="Webb, Watt W" uniqKey="Webb W" first="Watt W" last="Webb">Watt W. Webb</name></author><author><name sortKey="Pollack, Lois" sort="Pollack, Lois" uniqKey="Pollack L" first="Lois" last="Pollack">Lois Pollack</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Single-Stranded (chemistry)</term><term>Fluorescence Resonance Energy Transfer</term><term>Ions</term><term>Magnesium Chloride (pharmacology)</term><term>Models, Molecular</term><term>Osmolar Concentration</term><term>Pliability (drug effects)</term><term>RNA (chemistry)</term><term>Scattering, Small Angle</term><term>Sodium Chloride (pharmacology)</term><term>X-Ray Diffraction</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN simple brin ()</term><term>ARN ()</term><term>Chlorure de magnésium (pharmacologie)</term><term>Chlorure de sodium (pharmacologie)</term><term>Concentration osmolaire</term><term>Diffraction des rayons X</term><term>Diffusion aux petits angles</term><term>Flexibilité ()</term><term>Ions</term><term>Modèles moléculaires</term><term>Transfert d'énergie par résonance de fluorescence</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Single-Stranded</term><term>RNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Magnesium Chloride</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Pliability</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chlorure de magnésium</term><term>Chlorure de sodium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fluorescence Resonance Energy Transfer</term><term>Ions</term><term>Models, Molecular</term><term>Osmolar Concentration</term><term>Scattering, Small Angle</term><term>X-Ray Diffraction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN simple brin</term><term>ARN</term><term>Concentration osmolaire</term><term>Diffraction des rayons X</term><term>Diffusion aux petits angles</term><term>Flexibilité</term><term>Ions</term><term>Modèles moléculaires</term><term>Transfert d'énergie par résonance de fluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dynamic RNA molecules carry out essential processes in the cell including translation and splicing. Base-pair interactions stabilize RNA into relatively rigid structures, while flexible non-base-paired regions allow RNA to undergo conformational changes required for function. To advance our understanding of RNA folding and dynamics it is critical to know the flexibility of these un-base-paired regions and how it depends on counterions. Yet, information about nucleic acid polymer properties is mainly derived from studies of ssDNA. Here we measure the persistence lengths (l(p)) of ssRNA. We observe valence and ionic strength-dependent differences in l(p) in a direct comparison between 40-mers of deoxythymidylate (dT(40)) and uridylate (rU(40)) measured using the powerful combination of SAXS and smFRET. We also show that nucleic acid flexibility is influenced by local environment (an adjoining double helix). Our results illustrate the complex interplay between conformation and ion environment that modulates nucleic acid function in vivo.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22203973</PMID><DateCompleted><Year>2012</Year><Month>03</Month><Day>21</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>109</Volume><Issue>3</Issue><PubDate><Year>2012</Year><Month>Jan</Month><Day>17</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation></Journal><ArticleTitle>Ionic strength-dependent persistence lengths of single-stranded RNA and DNA.</ArticleTitle><Pagination><MedlinePgn>799-804</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.1119057109</ELocationID><Abstract><AbstractText>Dynamic RNA molecules carry out essential processes in the cell including translation and splicing. Base-pair interactions stabilize RNA into relatively rigid structures, while flexible non-base-paired regions allow RNA to undergo conformational changes required for function. To advance our understanding of RNA folding and dynamics it is critical to know the flexibility of these un-base-paired regions and how it depends on counterions. Yet, information about nucleic acid polymer properties is mainly derived from studies of ssDNA. Here we measure the persistence lengths (l(p)) of ssRNA. We observe valence and ionic strength-dependent differences in l(p) in a direct comparison between 40-mers of deoxythymidylate (dT(40)) and uridylate (rU(40)) measured using the powerful combination of SAXS and smFRET. We also show that nucleic acid flexibility is influenced by local environment (an adjoining double helix). 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ValidYN="Y"><LastName>Pollack</LastName><ForeName>Lois</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01-GM085062</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 GM085062</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>T32-GM008267</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>T32 GM008267</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>DMR0225180</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType 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Meisburger</name><name sortKey="Pabit, Suzette A" sort="Pabit, Suzette A" uniqKey="Pabit S" first="Suzette A" last="Pabit">Suzette A. Pabit</name><name sortKey="Pollack, Lois" sort="Pollack, Lois" uniqKey="Pollack L" first="Lois" last="Pollack">Lois Pollack</name><name sortKey="Sutton, Julie L" sort="Sutton, Julie L" uniqKey="Sutton J" first="Julie L" last="Sutton">Julie L. Sutton</name><name sortKey="Webb, Watt W" sort="Webb, Watt W" uniqKey="Webb W" first="Watt W" last="Webb">Watt W. Webb</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Chen, Huimin" sort="Chen, Huimin" uniqKey="Chen H" first="Huimin" last="Chen">Huimin Chen</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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