Gene sensors based on peptide nucleic acid (PNA) probes: relationship between sensor sensitivity and probe/target duplex stability.
Identifieur interne : 000376 ( Ncbi/Curation ); précédent : 000375; suivant : 000377Gene sensors based on peptide nucleic acid (PNA) probes: relationship between sensor sensitivity and probe/target duplex stability.
Auteurs : Hiroshi Aoki [Japon] ; Hiroaki TaoSource :
- The Analyst [ 0003-2654 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN.
- génétique : Acides nucléiques peptidiques.
- instrumentation : Animaux, Hétéroduplexes d'acides nucléiques, Matrices (génétique), Mésappariement de bases, Séquençage par oligonucléotides en batterie, Électrochimie, Électrodes.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA.
- chemical , genetics : Peptide Nucleic Acids.
- instrumentation : Electrochemistry.
- methods : Electrochemistry.
- Animals, Base Pair Mismatch, Electrodes, Nucleic Acid Heteroduplexes, Oligonucleotide Array Sequence Analysis, Templates, Genetic.
Abstract
Gene sensors based on peptide nucleic acid (PNA) probes were prepared and the relationship between sensor sensitivity and the duplex stability of the probe PNAs and target complementary DNAs was studied using five synthesized PNAs (10-, 15-, 17-, 20-, and 22-mers). It was found that the association constants for the probe PNA/target DNA pairs depend not only on the length but also on the base pair sequence, and that the trend in the sensor responses was the same as that in the association constants for the corresponding pairs. In addition, by using two kinds of probe PNAs with different lengths, it was demonstrated that fabrication of sensors based on probe PNAs with comparable association constants yielded similar response curves and sensor sensitivities.
DOI: 10.1039/b507121f
PubMed: 16222367
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pubmed:16222367Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8569, Japan. aoki-h@aist.go.jp</nlm:affiliation>
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<series><title level="j">The Analyst</title>
<idno type="ISSN">0003-2654</idno>
<imprint><date when="2005" type="published">2005</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Base Pair Mismatch</term>
<term>DNA (analysis)</term>
<term>Electrochemistry (instrumentation)</term>
<term>Electrochemistry (methods)</term>
<term>Electrodes</term>
<term>Nucleic Acid Heteroduplexes</term>
<term>Oligonucleotide Array Sequence Analysis</term>
<term>Peptide Nucleic Acids (genetics)</term>
<term>Templates, Genetic</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term>
<term>Acides nucléiques peptidiques (génétique)</term>
<term>Animaux</term>
<term>Hétéroduplexes d'acides nucléiques</term>
<term>Matrices (génétique)</term>
<term>Mésappariement de bases</term>
<term>Séquençage par oligonucléotides en batterie</term>
<term>Électrochimie ()</term>
<term>Électrochimie (instrumentation)</term>
<term>Électrodes</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptide Nucleic Acids</term>
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<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Acides nucléiques peptidiques</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Electrochemistry</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrochemistry</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Base Pair Mismatch</term>
<term>Electrodes</term>
<term>Nucleic Acid Heteroduplexes</term>
<term>Oligonucleotide Array Sequence Analysis</term>
<term>Templates, Genetic</term>
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<keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Animaux</term>
<term>Hétéroduplexes d'acides nucléiques</term>
<term>Matrices (génétique)</term>
<term>Mésappariement de bases</term>
<term>Séquençage par oligonucléotides en batterie</term>
<term>Électrochimie</term>
<term>Électrodes</term>
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<front><div type="abstract" xml:lang="en">Gene sensors based on peptide nucleic acid (PNA) probes were prepared and the relationship between sensor sensitivity and the duplex stability of the probe PNAs and target complementary DNAs was studied using five synthesized PNAs (10-, 15-, 17-, 20-, and 22-mers). It was found that the association constants for the probe PNA/target DNA pairs depend not only on the length but also on the base pair sequence, and that the trend in the sensor responses was the same as that in the association constants for the corresponding pairs. In addition, by using two kinds of probe PNAs with different lengths, it was demonstrated that fabrication of sensors based on probe PNAs with comparable association constants yielded similar response curves and sensor sensitivities.</div>
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