Spectroscopic studies of single-stranded DNA ligands and oxazole yellow dyes.
Identifieur interne : 002B13 ( Ncbi/Checkpoint ); précédent : 002B12; suivant : 002B14Spectroscopic studies of single-stranded DNA ligands and oxazole yellow dyes.
Auteurs : K H Abramo [États-Unis] ; J B Pitner ; L B McgownSource :
- Biospectroscopy [ 1075-4261 ] ; 1998.
Descripteurs français
- KwdFr :
- ADN simple brin (), ADN simple brin (métabolisme), Analyse de Fourier, Benzoxazoles (), Benzoxazoles (métabolisme), Cinétique, Colorants fluorescents (), Colorants fluorescents (métabolisme), Conformation d'acide nucléique, Dichroïsme circulaire, Fixation compétitive, Ligands, Polarisation de fluorescence, Quinoléines (), Quinoléines (métabolisme), Relation structure-activité, Spectrométrie de fluorescence, Séquence nucléotidique.
- MESH :
- métabolisme : ADN simple brin, Benzoxazoles, Colorants fluorescents, Quinoléines.
- ADN simple brin, Analyse de Fourier, Benzoxazoles, Cinétique, Colorants fluorescents, Conformation d'acide nucléique, Dichroïsme circulaire, Fixation compétitive, Ligands, Polarisation de fluorescence, Quinoléines, Relation structure-activité, Spectrométrie de fluorescence, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, Benzoxazoles (chemistry), Benzoxazoles (metabolism), Binding, Competitive, Circular Dichroism, DNA, Single-Stranded (chemistry), DNA, Single-Stranded (metabolism), Fluorescence Polarization, Fluorescent Dyes (chemistry), Fluorescent Dyes (metabolism), Fourier Analysis, Kinetics, Ligands, Nucleic Acid Conformation, Quinolines (chemistry), Quinolines (metabolism), Spectrometry, Fluorescence, Structure-Activity Relationship.
- MESH :
- chemical , chemistry : Benzoxazoles, DNA, Single-Stranded, Fluorescent Dyes, Quinolines.
- chemical , metabolism : Benzoxazoles, DNA, Single-Stranded, Fluorescent Dyes, Quinolines.
- Base Sequence, Binding, Competitive, Circular Dichroism, Fluorescence Polarization, Fourier Analysis, Kinetics, Ligands, Nucleic Acid Conformation, Spectrometry, Fluorescence, Structure-Activity Relationship.
Abstract
Interactions between short single-stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G-quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO-pro-1 iodide (YO) and YOYO-1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G-quartet forming ligands, a 20-mer of sequence 5'-GGTTTTGGTTTTGGTTTTGG-3', shows distinctly different interactions from the other three ligands, all of which are 15-mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single-stranded DNA.
DOI: 10.1002/(SICI)1520-6343(1998)4:1%3C27::AID-BSPY3%3E3.0.CO;2-P
PubMed: 9547012
Affiliations:
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pubmed:9547012Le document en format XML
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when="1998">1998</date><idno type="RBID">pubmed:9547012</idno><idno type="pmid">9547012</idno><idno type="doi">10.1002/(SICI)1520-6343(1998)4:1%3C27::AID-BSPY3%3E3.0.CO;2-P</idno><idno type="wicri:Area/PubMed/Corpus">002695</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002695</idno><idno type="wicri:Area/PubMed/Curation">002695</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002695</idno><idno type="wicri:Area/PubMed/Checkpoint">002509</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002509</idno><idno type="wicri:Area/Ncbi/Merge">002B13</idno><idno type="wicri:Area/Ncbi/Curation">002B13</idno><idno type="wicri:Area/Ncbi/Checkpoint">002B13</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Spectroscopic studies of single-stranded DNA ligands and oxazole yellow dyes.</title><author><name sortKey="Abramo, K H" sort="Abramo, K H" uniqKey="Abramo K" first="K H" last="Abramo">K H Abramo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Duke University, Durham, North Carolina 27708-0346, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, Duke University, Durham, North Carolina 27708-0346</wicri:regionArea><wicri:noRegion>North Carolina 27708-0346</wicri:noRegion></affiliation></author><author><name sortKey="Pitner, J B" sort="Pitner, J B" uniqKey="Pitner J" first="J B" last="Pitner">J B Pitner</name></author><author><name sortKey="Mcgown, L B" sort="Mcgown, L B" uniqKey="Mcgown L" first="L B" last="Mcgown">L B Mcgown</name></author></analytic><series><title level="j">Biospectroscopy</title><idno type="ISSN">1075-4261</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Benzoxazoles (chemistry)</term><term>Benzoxazoles (metabolism)</term><term>Binding, Competitive</term><term>Circular Dichroism</term><term>DNA, Single-Stranded (chemistry)</term><term>DNA, Single-Stranded (metabolism)</term><term>Fluorescence Polarization</term><term>Fluorescent Dyes (chemistry)</term><term>Fluorescent Dyes (metabolism)</term><term>Fourier Analysis</term><term>Kinetics</term><term>Ligands</term><term>Nucleic Acid Conformation</term><term>Quinolines (chemistry)</term><term>Quinolines (metabolism)</term><term>Spectrometry, Fluorescence</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN simple brin ()</term><term>ADN simple brin (métabolisme)</term><term>Analyse de Fourier</term><term>Benzoxazoles ()</term><term>Benzoxazoles (métabolisme)</term><term>Cinétique</term><term>Colorants fluorescents ()</term><term>Colorants fluorescents (métabolisme)</term><term>Conformation d'acide nucléique</term><term>Dichroïsme circulaire</term><term>Fixation compétitive</term><term>Ligands</term><term>Polarisation de fluorescence</term><term>Quinoléines ()</term><term>Quinoléines (métabolisme)</term><term>Relation structure-activité</term><term>Spectrométrie de fluorescence</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Benzoxazoles</term><term>DNA, Single-Stranded</term><term>Fluorescent Dyes</term><term>Quinolines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Benzoxazoles</term><term>DNA, Single-Stranded</term><term>Fluorescent Dyes</term><term>Quinolines</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN simple brin</term><term>Benzoxazoles</term><term>Colorants fluorescents</term><term>Quinoléines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Binding, Competitive</term><term>Circular Dichroism</term><term>Fluorescence Polarization</term><term>Fourier Analysis</term><term>Kinetics</term><term>Ligands</term><term>Nucleic Acid Conformation</term><term>Spectrometry, Fluorescence</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN simple brin</term><term>Analyse de Fourier</term><term>Benzoxazoles</term><term>Cinétique</term><term>Colorants fluorescents</term><term>Conformation d'acide nucléique</term><term>Dichroïsme circulaire</term><term>Fixation compétitive</term><term>Ligands</term><term>Polarisation de fluorescence</term><term>Quinoléines</term><term>Relation structure-activité</term><term>Spectrométrie de fluorescence</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Interactions between short single-stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G-quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO-pro-1 iodide (YO) and YOYO-1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G-quartet forming ligands, a 20-mer of sequence 5'-GGTTTTGGTTTTGGTTTTGG-3', shows distinctly different interactions from the other three ligands, all of which are 15-mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single-stranded DNA.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Mcgown, L B" sort="Mcgown, L B" uniqKey="Mcgown L" first="L B" last="Mcgown">L B Mcgown</name><name sortKey="Pitner, J B" sort="Pitner, J B" uniqKey="Pitner J" first="J B" last="Pitner">J B Pitner</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Abramo, K H" sort="Abramo, K H" uniqKey="Abramo K" first="K H" last="Abramo">K H Abramo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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