Spectroscopic studies of single-stranded DNA ligands and oxazole yellow dyes.
Identifieur interne : 002695 ( PubMed/Curation ); précédent : 002694; suivant : 002696Spectroscopic studies of single-stranded DNA ligands and oxazole yellow dyes.
Auteurs : K H Abramo [États-Unis] ; J B Pitner ; L B McgownSource :
- Biospectroscopy [ 1075-4261 ] ; 1998.
Descripteurs français
- KwdFr :
- ADN simple brin (), ADN simple brin (métabolisme), Analyse de Fourier, Benzoxazoles (), Benzoxazoles (métabolisme), Cinétique, Colorants fluorescents (), Colorants fluorescents (métabolisme), Conformation d'acide nucléique, Dichroïsme circulaire, Fixation compétitive, Ligands, Polarisation de fluorescence, Quinoléines (), Quinoléines (métabolisme), Relation structure-activité, Spectrométrie de fluorescence, Séquence nucléotidique.
- MESH :
- métabolisme : ADN simple brin, Benzoxazoles, Colorants fluorescents, Quinoléines.
- ADN simple brin, Analyse de Fourier, Benzoxazoles, Cinétique, Colorants fluorescents, Conformation d'acide nucléique, Dichroïsme circulaire, Fixation compétitive, Ligands, Polarisation de fluorescence, Quinoléines, Relation structure-activité, Spectrométrie de fluorescence, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, Benzoxazoles (chemistry), Benzoxazoles (metabolism), Binding, Competitive, Circular Dichroism, DNA, Single-Stranded (chemistry), DNA, Single-Stranded (metabolism), Fluorescence Polarization, Fluorescent Dyes (chemistry), Fluorescent Dyes (metabolism), Fourier Analysis, Kinetics, Ligands, Nucleic Acid Conformation, Quinolines (chemistry), Quinolines (metabolism), Spectrometry, Fluorescence, Structure-Activity Relationship.
- MESH :
- chemical , chemistry : Benzoxazoles, DNA, Single-Stranded, Fluorescent Dyes, Quinolines.
- chemical , metabolism : Benzoxazoles, DNA, Single-Stranded, Fluorescent Dyes, Quinolines.
- Base Sequence, Binding, Competitive, Circular Dichroism, Fluorescence Polarization, Fourier Analysis, Kinetics, Ligands, Nucleic Acid Conformation, Spectrometry, Fluorescence, Structure-Activity Relationship.
Abstract
Interactions between short single-stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G-quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO-pro-1 iodide (YO) and YOYO-1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G-quartet forming ligands, a 20-mer of sequence 5'-GGTTTTGGTTTTGGTTTTGG-3', shows distinctly different interactions from the other three ligands, all of which are 15-mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single-stranded DNA.
DOI: 10.1002/(SICI)1520-6343(1998)4:1%3C27::AID-BSPY3%3E3.0.CO;2-P
PubMed: 9547012
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Analysis</term><term>Kinetics</term><term>Ligands</term><term>Nucleic Acid Conformation</term><term>Quinolines (chemistry)</term><term>Quinolines (metabolism)</term><term>Spectrometry, Fluorescence</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN simple brin ()</term><term>ADN simple brin (métabolisme)</term><term>Analyse de Fourier</term><term>Benzoxazoles ()</term><term>Benzoxazoles (métabolisme)</term><term>Cinétique</term><term>Colorants fluorescents ()</term><term>Colorants fluorescents (métabolisme)</term><term>Conformation d'acide nucléique</term><term>Dichroïsme circulaire</term><term>Fixation compétitive</term><term>Ligands</term><term>Polarisation de fluorescence</term><term>Quinoléines ()</term><term>Quinoléines (métabolisme)</term><term>Relation structure-activité</term><term>Spectrométrie de fluorescence</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Benzoxazoles</term><term>DNA, Single-Stranded</term><term>Fluorescent Dyes</term><term>Quinolines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Benzoxazoles</term><term>DNA, Single-Stranded</term><term>Fluorescent Dyes</term><term>Quinolines</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN simple brin</term><term>Benzoxazoles</term><term>Colorants fluorescents</term><term>Quinoléines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Binding, Competitive</term><term>Circular Dichroism</term><term>Fluorescence Polarization</term><term>Fourier Analysis</term><term>Kinetics</term><term>Ligands</term><term>Nucleic Acid Conformation</term><term>Spectrometry, Fluorescence</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN simple brin</term><term>Analyse de Fourier</term><term>Benzoxazoles</term><term>Cinétique</term><term>Colorants fluorescents</term><term>Conformation d'acide nucléique</term><term>Dichroïsme circulaire</term><term>Fixation compétitive</term><term>Ligands</term><term>Polarisation de fluorescence</term><term>Quinoléines</term><term>Relation structure-activité</term><term>Spectrométrie de fluorescence</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Interactions between short single-stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G-quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO-pro-1 iodide (YO) and YOYO-1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G-quartet forming ligands, a 20-mer of sequence 5'-GGTTTTGGTTTTGGTTTTGG-3', shows distinctly different interactions from the other three ligands, all of which are 15-mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single-stranded DNA.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9547012</PMID><DateCompleted><Year>1998</Year><Month>04</Month><Day>16</Day></DateCompleted><DateRevised><Year>2018</Year><Month>10</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1075-4261</ISSN><JournalIssue CitedMedium="Print"><Volume>4</Volume><Issue>1</Issue><PubDate><Year>1998</Year></PubDate></JournalIssue><Title>Biospectroscopy</Title><ISOAbbreviation>Biospectroscopy</ISOAbbreviation></Journal><ArticleTitle>Spectroscopic studies of single-stranded DNA ligands and oxazole yellow dyes.</ArticleTitle><Pagination><MedlinePgn>27-35</MedlinePgn></Pagination><Abstract><AbstractText>Interactions between short single-stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G-quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO-pro-1 iodide (YO) and YOYO-1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G-quartet forming ligands, a 20-mer of sequence 5'-GGTTTTGGTTTTGGTTTTGG-3', shows distinctly different interactions from the other three ligands, all of which are 15-mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single-stranded DNA.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Abramo</LastName><ForeName>K H</ForeName><Initials>KH</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Duke University, Durham, North Carolina 27708-0346, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pitner</LastName><ForeName>J B</ForeName><Initials>JB</Initials></Author><Author ValidYN="Y"><LastName>McGown</LastName><ForeName>L B</ForeName><Initials>LB</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>T32 ES007031</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>T32ES07031-17</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biospectroscopy</MedlineTA><NlmUniqueID>9605413</NlmUniqueID><ISSNLinking>1075-4261</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001583">Benzoxazoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004277">DNA, Single-Stranded</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005456">Fluorescent Dyes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008024">Ligands</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011804">Quinolines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C097015">oxazole yellow</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001583" MajorTopicYN="N">Benzoxazoles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001667" MajorTopicYN="N">Binding, Competitive</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002942" MajorTopicYN="N">Circular Dichroism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004277" MajorTopicYN="N">DNA, Single-Stranded</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005454" MajorTopicYN="N">Fluorescence Polarization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005456" MajorTopicYN="N">Fluorescent Dyes</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005583" MajorTopicYN="N">Fourier Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008024" MajorTopicYN="N">Ligands</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009690" MajorTopicYN="N">Nucleic Acid Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011804" MajorTopicYN="N">Quinolines</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013050" MajorTopicYN="Y">Spectrometry, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013329" MajorTopicYN="N">Structure-Activity Relationship</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>4</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1998</Year><Month>4</Month><Day>18</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1998</Year><Month>4</Month><Day>18</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9547012</ArticleId><ArticleId IdType="doi">10.1002/(SICI)1520-6343(1998)4:1%3C27::AID-BSPY3%3E3.0.CO;2-P</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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