Linear mitochondrial genome organization in vivo in the genus Pythium.
Identifieur interne : 002A04 ( Ncbi/Checkpoint ); précédent : 002A03; suivant : 002A05Linear mitochondrial genome organization in vivo in the genus Pythium.
Auteurs : F N Martin [États-Unis]Source :
- Current genetics [ 0172-8083 ] ; 1995.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), ADN mitochondrial (génétique), Adenosine triphosphatases (génétique), Alignement de séquences, Cartographie chromosomique, Données de séquences moléculaires, Génome fongique, Humains, Protéines fongiques (génétique), Protéines végétales (génétique), Pythium (génétique), Spécificité d'espèce, Séquence d'acides aminés, Séquence nucléotidique, Électrophorèse en champ pulsé.
- MESH :
- génétique : ADN fongique, ADN mitochondrial, Adenosine triphosphatases, Protéines fongiques, Protéines végétales, Pythium.
- Alignement de séquences, Cartographie chromosomique, Données de séquences moléculaires, Génome fongique, Humains, Spécificité d'espèce, Séquence d'acides aminés, Séquence nucléotidique, Électrophorèse en champ pulsé.
English descriptors
- KwdEn :
- Adenosine Triphosphatases (genetics), Amino Acid Sequence, Base Sequence, Chromosome Mapping, DNA, Fungal (genetics), DNA, Mitochondrial (genetics), Electrophoresis, Gel, Pulsed-Field, Fungal Proteins (genetics), Genome, Fungal, Humans, Molecular Sequence Data, Plant Proteins (genetics), Pythium (genetics), Sequence Alignment, Species Specificity.
- MESH :
- chemical , genetics : Adenosine Triphosphatases, DNA, Fungal, DNA, Mitochondrial, Fungal Proteins, Plant Proteins.
- genetics : Pythium.
- Amino Acid Sequence, Base Sequence, Chromosome Mapping, Electrophoresis, Gel, Pulsed-Field, Genome, Fungal, Humans, Molecular Sequence Data, Sequence Alignment, Species Specificity.
Abstract
Pulsed-field gel electrophoresis (PFGE) of isolates of Pythium oligandrum with linear mitochondrial genomes revealed a distinct band in ethidium bromide-stained gels similar in size to values estimated by restriction mapping of mitochondrial DNA (mtDNA). Southern analysis confirmed that these bands were mtDNA and indicated that linear genomes were present in unit-length size as well as multimers. Isolates of this species with circular mtDNA restriction maps also had low levels of linear mono- and multimers visualized by Southern analysis of PFGE gels. Examination of 17 additional species revealed similar results; three species had distinct linear mtDNA bands in ethidium bromide-stained gels while the remainder had linear mono- and multi-mers in lower amounts detected only by Southern analysis. Sequence analysis of an isolate of P. oligandrum with a primarily circular mitochondrial genomic map and a low amount of linear molecules revealed that the small unique region of the circular map (which corresponded to the terminal region of linear genomes) was flanked by palindromic intrastrand complementary sequences separated by a unique 194-bp sequence. Sequences with similarity to ATPase9 coding regions from other organisms were located adjacent to this region. Sequences with similarity to mitochondrial origins of replication and autonomously replicating sequences were also located in this region: their potential involvement in the generation of linear molecules is discussed.
DOI: 10.1007/bf00309781
PubMed: 8529268
Affiliations:
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32611</wicri:regionArea><wicri:noRegion>Gainesville 32611</wicri:noRegion></affiliation></author></analytic><series><title level="j">Current genetics</title><idno type="ISSN">0172-8083</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphatases (genetics)</term><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Chromosome Mapping</term><term>DNA, Fungal (genetics)</term><term>DNA, Mitochondrial (genetics)</term><term>Electrophoresis, Gel, Pulsed-Field</term><term>Fungal Proteins (genetics)</term><term>Genome, Fungal</term><term>Humans</term><term>Molecular Sequence Data</term><term>Plant Proteins (genetics)</term><term>Pythium (genetics)</term><term>Sequence Alignment</term><term>Species Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term><term>ADN mitochondrial (génétique)</term><term>Adenosine triphosphatases (génétique)</term><term>Alignement de séquences</term><term>Cartographie chromosomique</term><term>Données de séquences moléculaires</term><term>Génome fongique</term><term>Humains</term><term>Protéines fongiques (génétique)</term><term>Protéines végétales (génétique)</term><term>Pythium (génétique)</term><term>Spécificité d'espèce</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Électrophorèse en champ pulsé</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Adenosine Triphosphatases</term><term>DNA, Fungal</term><term>DNA, Mitochondrial</term><term>Fungal Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pythium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>ADN mitochondrial</term><term>Adenosine triphosphatases</term><term>Protéines fongiques</term><term>Protéines végétales</term><term>Pythium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Chromosome Mapping</term><term>Electrophoresis, Gel, Pulsed-Field</term><term>Genome, Fungal</term><term>Humans</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Cartographie chromosomique</term><term>Données de séquences moléculaires</term><term>Génome fongique</term><term>Humains</term><term>Spécificité d'espèce</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Électrophorèse en champ pulsé</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pulsed-field gel electrophoresis (PFGE) of isolates of Pythium oligandrum with linear mitochondrial genomes revealed a distinct band in ethidium bromide-stained gels similar in size to values estimated by restriction mapping of mitochondrial DNA (mtDNA). Southern analysis confirmed that these bands were mtDNA and indicated that linear genomes were present in unit-length size as well as multimers. Isolates of this species with circular mtDNA restriction maps also had low levels of linear mono- and multimers visualized by Southern analysis of PFGE gels. Examination of 17 additional species revealed similar results; three species had distinct linear mtDNA bands in ethidium bromide-stained gels while the remainder had linear mono- and multi-mers in lower amounts detected only by Southern analysis. Sequence analysis of an isolate of P. oligandrum with a primarily circular mitochondrial genomic map and a low amount of linear molecules revealed that the small unique region of the circular map (which corresponded to the terminal region of linear genomes) was flanked by palindromic intrastrand complementary sequences separated by a unique 194-bp sequence. Sequences with similarity to ATPase9 coding regions from other organisms were located adjacent to this region. Sequences with similarity to mitochondrial origins of replication and autonomously replicating sequences were also located in this region: their potential involvement in the generation of linear molecules is discussed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Martin, F N" sort="Martin, F N" uniqKey="Martin F" first="F N" last="Martin">F N Martin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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