Comparative binding properties of metallobleomycins with DNA 10-mers.
Identifieur interne : 000088 ( Ncbi/Checkpoint ); précédent : 000087; suivant : 000089Comparative binding properties of metallobleomycins with DNA 10-mers.
Auteurs : W. Li [États-Unis] ; C. Zhao ; C. Xia ; W E Antholine ; D H PeteringSource :
- Biochemistry [ 0006-2960 ] ; 2001.
Descripteurs français
- KwdFr :
- Adduits à l'ADN (), Bléomycine (), Bléomycine (analogues et dérivés), Cobalt (), Composés organométalliques (), Cuivre (), Dichroïsme circulaire, Fer (), Métaux (), Oligodésoxyribonucléotides (), Résonance magnétique nucléaire biomoléculaire, Sites de fixation, Spectrométrie de fluorescence, Zinc ().
- MESH :
English descriptors
- KwdEn :
- Binding Sites, Bleomycin (analogs & derivatives), Bleomycin (chemistry), Circular Dichroism, Cobalt (chemistry), Copper (chemistry), DNA Adducts (chemistry), Iron (chemistry), Metals (chemistry), Nuclear Magnetic Resonance, Biomolecular, Oligodeoxyribonucleotides (chemistry), Organometallic Compounds (chemistry), Spectrometry, Fluorescence, Zinc (chemistry).
- MESH :
- chemical , analogs & derivatives : Bleomycin.
- chemical , chemistry : Bleomycin, Cobalt, Copper, DNA Adducts, Iron, Metals, Oligodeoxyribonucleotides, Organometallic Compounds, Zinc.
- Binding Sites, Circular Dichroism, Nuclear Magnetic Resonance, Biomolecular, Spectrometry, Fluorescence.
Abstract
Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2), ZnBlm A(2), Cu(II)Blm A(2), Fe(III)Blm A(2), and HO(2)-Co(III)Blm A(2). Generally, the metallobleomycins were bound with a modestly higher affinity to I. One-dimensional (1)H NMR spectra of the imino proton region of I in the presence of this series of compounds revealed that Blm and Zn- and CuBlm bind in fast exchange on the NMR time scale, while the Fe and Co complexes bind in slow exchange. Blm, ZnBlm, and Cu(II)Blm caused little perturbation of the UV circular dichroism spectrum of I or II. In contrast, Fe(III)Blm and HO(2)-Co(III)Blm induced hypochromic effects in the CD spectrum of I and altered the spectrum of II to a smaller extent. On the basis of these results, the DNA binding structures and properties of Blm A(2), ZnBlm A(2), and CuBlm A(2) differ substantially from those of Fe(III)Blm A(2) and HO(2)-Co(III)Blm A(2).
DOI: 10.1021/bi001915t
PubMed: 11412110
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative binding properties of metallobleomycins with DNA 10-mers.</title><author><name sortKey="Li, W" sort="Li, W" uniqKey="Li W" first="W" last="Li">W. Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, Wisconsin 53201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, Wisconsin 53201</wicri:regionArea><wicri:noRegion>Wisconsin 53201</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, C" sort="Zhao, C" uniqKey="Zhao C" first="C" last="Zhao">C. Zhao</name></author><author><name sortKey="Xia, C" sort="Xia, C" uniqKey="Xia C" first="C" last="Xia">C. Xia</name></author><author><name sortKey="Antholine, W E" sort="Antholine, W E" uniqKey="Antholine W" first="W E" last="Antholine">W E Antholine</name></author><author><name sortKey="Petering, D H" sort="Petering, D H" uniqKey="Petering D" first="D H" last="Petering">D H Petering</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11412110</idno><idno type="pmid">11412110</idno><idno type="doi">10.1021/bi001915t</idno><idno type="wicri:Area/PubMed/Corpus">002557</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002557</idno><idno type="wicri:Area/PubMed/Curation">002557</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002557</idno><idno type="wicri:Area/PubMed/Checkpoint">002421</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002421</idno><idno type="wicri:Area/Ncbi/Merge">000088</idno><idno type="wicri:Area/Ncbi/Curation">000088</idno><idno type="wicri:Area/Ncbi/Checkpoint">000088</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative binding properties of metallobleomycins with DNA 10-mers.</title><author><name sortKey="Li, W" sort="Li, W" uniqKey="Li W" first="W" last="Li">W. Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, Wisconsin 53201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, Wisconsin 53201</wicri:regionArea><wicri:noRegion>Wisconsin 53201</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, C" sort="Zhao, C" uniqKey="Zhao C" first="C" last="Zhao">C. Zhao</name></author><author><name sortKey="Xia, C" sort="Xia, C" uniqKey="Xia C" first="C" last="Xia">C. Xia</name></author><author><name sortKey="Antholine, W E" sort="Antholine, W E" uniqKey="Antholine W" first="W E" last="Antholine">W E Antholine</name></author><author><name sortKey="Petering, D H" sort="Petering, D H" uniqKey="Petering D" first="D H" last="Petering">D H Petering</name></author></analytic><series><title level="j">Biochemistry</title><idno type="ISSN">0006-2960</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding Sites</term><term>Bleomycin (analogs & derivatives)</term><term>Bleomycin (chemistry)</term><term>Circular Dichroism</term><term>Cobalt (chemistry)</term><term>Copper (chemistry)</term><term>DNA Adducts (chemistry)</term><term>Iron (chemistry)</term><term>Metals (chemistry)</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Oligodeoxyribonucleotides (chemistry)</term><term>Organometallic Compounds (chemistry)</term><term>Spectrometry, Fluorescence</term><term>Zinc (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adduits à l'ADN ()</term><term>Bléomycine ()</term><term>Bléomycine (analogues et dérivés)</term><term>Cobalt ()</term><term>Composés organométalliques ()</term><term>Cuivre ()</term><term>Dichroïsme circulaire</term><term>Fer ()</term><term>Métaux ()</term><term>Oligodésoxyribonucléotides ()</term><term>Résonance magnétique nucléaire biomoléculaire</term><term>Sites de fixation</term><term>Spectrométrie de fluorescence</term><term>Zinc ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Bleomycin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Bleomycin</term><term>Cobalt</term><term>Copper</term><term>DNA Adducts</term><term>Iron</term><term>Metals</term><term>Oligodeoxyribonucleotides</term><term>Organometallic Compounds</term><term>Zinc</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Bléomycine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term><term>Circular Dichroism</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Spectrometry, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adduits à l'ADN</term><term>Bléomycine</term><term>Cobalt</term><term>Composés organométalliques</term><term>Cuivre</term><term>Dichroïsme circulaire</term><term>Fer</term><term>Métaux</term><term>Oligodésoxyribonucléotides</term><term>Résonance magnétique nucléaire biomoléculaire</term><term>Sites de fixation</term><term>Spectrométrie de fluorescence</term><term>Zinc</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2), ZnBlm A(2), Cu(II)Blm A(2), Fe(III)Blm A(2), and HO(2)-Co(III)Blm A(2). Generally, the metallobleomycins were bound with a modestly higher affinity to I. One-dimensional (1)H NMR spectra of the imino proton region of I in the presence of this series of compounds revealed that Blm and Zn- and CuBlm bind in fast exchange on the NMR time scale, while the Fe and Co complexes bind in slow exchange. Blm, ZnBlm, and Cu(II)Blm caused little perturbation of the UV circular dichroism spectrum of I or II. In contrast, Fe(III)Blm and HO(2)-Co(III)Blm induced hypochromic effects in the CD spectrum of I and altered the spectrum of II to a smaller extent. On the basis of these results, the DNA binding structures and properties of Blm A(2), ZnBlm A(2), and CuBlm A(2) differ substantially from those of Fe(III)Blm A(2) and HO(2)-Co(III)Blm A(2).</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Antholine, W E" sort="Antholine, W E" uniqKey="Antholine W" first="W E" last="Antholine">W E Antholine</name><name sortKey="Petering, D H" sort="Petering, D H" uniqKey="Petering D" first="D H" last="Petering">D H Petering</name><name sortKey="Xia, C" sort="Xia, C" uniqKey="Xia C" first="C" last="Xia">C. Xia</name><name sortKey="Zhao, C" sort="Zhao, C" uniqKey="Zhao C" first="C" last="Zhao">C. Zhao</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Li, W" sort="Li, W" uniqKey="Li W" first="W" last="Li">W. Li</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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