Comparative binding properties of metallobleomycins with DNA 10-mers.
Identifieur interne : 002557 ( PubMed/Corpus ); précédent : 002556; suivant : 002558Comparative binding properties of metallobleomycins with DNA 10-mers.
Auteurs : W. Li ; C. Zhao ; C. Xia ; W E Antholine ; D H PeteringSource :
- Biochemistry [ 0006-2960 ] ; 2001.
English descriptors
- KwdEn :
- Binding Sites, Bleomycin (analogs & derivatives), Bleomycin (chemistry), Circular Dichroism, Cobalt (chemistry), Copper (chemistry), DNA Adducts (chemistry), Iron (chemistry), Metals (chemistry), Nuclear Magnetic Resonance, Biomolecular, Oligodeoxyribonucleotides (chemistry), Organometallic Compounds (chemistry), Spectrometry, Fluorescence, Zinc (chemistry).
- MESH :
- chemical , analogs & derivatives : Bleomycin.
- chemical , chemistry : Bleomycin, Cobalt, Copper, DNA Adducts, Iron, Metals, Oligodeoxyribonucleotides, Organometallic Compounds, Zinc.
- Binding Sites, Circular Dichroism, Nuclear Magnetic Resonance, Biomolecular, Spectrometry, Fluorescence.
Abstract
Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2), ZnBlm A(2), Cu(II)Blm A(2), Fe(III)Blm A(2), and HO(2)-Co(III)Blm A(2). Generally, the metallobleomycins were bound with a modestly higher affinity to I. One-dimensional (1)H NMR spectra of the imino proton region of I in the presence of this series of compounds revealed that Blm and Zn- and CuBlm bind in fast exchange on the NMR time scale, while the Fe and Co complexes bind in slow exchange. Blm, ZnBlm, and Cu(II)Blm caused little perturbation of the UV circular dichroism spectrum of I or II. In contrast, Fe(III)Blm and HO(2)-Co(III)Blm induced hypochromic effects in the CD spectrum of I and altered the spectrum of II to a smaller extent. On the basis of these results, the DNA binding structures and properties of Blm A(2), ZnBlm A(2), and CuBlm A(2) differ substantially from those of Fe(III)Blm A(2) and HO(2)-Co(III)Blm A(2).
DOI: 10.1021/bi001915t
PubMed: 11412110
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pubmed:11412110Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative binding properties of metallobleomycins with DNA 10-mers.</title><author><name sortKey="Li, W" sort="Li, W" uniqKey="Li W" first="W" last="Li">W. Li</name><affiliation><nlm:affiliation>Department of Chemistry, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, Wisconsin 53201, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zhao, C" sort="Zhao, C" uniqKey="Zhao C" first="C" last="Zhao">C. Zhao</name></author><author><name sortKey="Xia, C" sort="Xia, C" uniqKey="Xia C" first="C" last="Xia">C. Xia</name></author><author><name sortKey="Antholine, W E" sort="Antholine, W E" uniqKey="Antholine W" first="W E" last="Antholine">W E Antholine</name></author><author><name sortKey="Petering, D H" sort="Petering, D H" uniqKey="Petering D" first="D H" last="Petering">D H Petering</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11412110</idno><idno type="pmid">11412110</idno><idno type="doi">10.1021/bi001915t</idno><idno type="wicri:Area/PubMed/Corpus">002557</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002557</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative binding properties of metallobleomycins with DNA 10-mers.</title><author><name sortKey="Li, W" sort="Li, W" uniqKey="Li W" first="W" last="Li">W. Li</name><affiliation><nlm:affiliation>Department of Chemistry, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, Wisconsin 53201, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zhao, C" sort="Zhao, C" uniqKey="Zhao C" first="C" last="Zhao">C. Zhao</name></author><author><name sortKey="Xia, C" sort="Xia, C" uniqKey="Xia C" first="C" last="Xia">C. Xia</name></author><author><name sortKey="Antholine, W E" sort="Antholine, W E" uniqKey="Antholine W" first="W E" last="Antholine">W E Antholine</name></author><author><name sortKey="Petering, D H" sort="Petering, D H" uniqKey="Petering D" first="D H" last="Petering">D H Petering</name></author></analytic><series><title level="j">Biochemistry</title><idno type="ISSN">0006-2960</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding Sites</term><term>Bleomycin (analogs & derivatives)</term><term>Bleomycin (chemistry)</term><term>Circular Dichroism</term><term>Cobalt (chemistry)</term><term>Copper (chemistry)</term><term>DNA Adducts (chemistry)</term><term>Iron (chemistry)</term><term>Metals (chemistry)</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Oligodeoxyribonucleotides (chemistry)</term><term>Organometallic Compounds (chemistry)</term><term>Spectrometry, Fluorescence</term><term>Zinc (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Bleomycin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Bleomycin</term><term>Cobalt</term><term>Copper</term><term>DNA Adducts</term><term>Iron</term><term>Metals</term><term>Oligodeoxyribonucleotides</term><term>Organometallic Compounds</term><term>Zinc</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term><term>Circular Dichroism</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Spectrometry, Fluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2), ZnBlm A(2), Cu(II)Blm A(2), Fe(III)Blm A(2), and HO(2)-Co(III)Blm A(2). Generally, the metallobleomycins were bound with a modestly higher affinity to I. One-dimensional (1)H NMR spectra of the imino proton region of I in the presence of this series of compounds revealed that Blm and Zn- and CuBlm bind in fast exchange on the NMR time scale, while the Fe and Co complexes bind in slow exchange. Blm, ZnBlm, and Cu(II)Blm caused little perturbation of the UV circular dichroism spectrum of I or II. In contrast, Fe(III)Blm and HO(2)-Co(III)Blm induced hypochromic effects in the CD spectrum of I and altered the spectrum of II to a smaller extent. On the basis of these results, the DNA binding structures and properties of Blm A(2), ZnBlm A(2), and CuBlm A(2) differ substantially from those of Fe(III)Blm A(2) and HO(2)-Co(III)Blm A(2).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11412110</PMID><DateCompleted><Year>2001</Year><Month>09</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-2960</ISSN><JournalIssue CitedMedium="Print"><Volume>40</Volume><Issue>25</Issue><PubDate><Year>2001</Year><Month>Jun</Month><Day>26</Day></PubDate></JournalIssue><Title>Biochemistry</Title><ISOAbbreviation>Biochemistry</ISOAbbreviation></Journal><ArticleTitle>Comparative binding properties of metallobleomycins with DNA 10-mers.</ArticleTitle><Pagination><MedlinePgn>7559-68</MedlinePgn></Pagination><Abstract><AbstractText>Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2), ZnBlm A(2), Cu(II)Blm A(2), Fe(III)Blm A(2), and HO(2)-Co(III)Blm A(2). Generally, the metallobleomycins were bound with a modestly higher affinity to I. One-dimensional (1)H NMR spectra of the imino proton region of I in the presence of this series of compounds revealed that Blm and Zn- and CuBlm bind in fast exchange on the NMR time scale, while the Fe and Co complexes bind in slow exchange. Blm, ZnBlm, and Cu(II)Blm caused little perturbation of the UV circular dichroism spectrum of I or II. In contrast, Fe(III)Blm and HO(2)-Co(III)Blm induced hypochromic effects in the CD spectrum of I and altered the spectrum of II to a smaller extent. On the basis of these results, the DNA binding structures and properties of Blm A(2), ZnBlm A(2), and CuBlm A(2) differ substantially from those of Fe(III)Blm A(2) and HO(2)-Co(III)Blm A(2).</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>W</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Chemistry, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, Wisconsin 53201, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Xia</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Antholine</LastName><ForeName>W E</ForeName><Initials>WE</Initials></Author><Author ValidYN="Y"><LastName>Petering</LastName><ForeName>D H</ForeName><Initials>DH</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>CA-22184</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochemistry</MedlineTA><NlmUniqueID>0370623</NlmUniqueID><ISSNLinking>0006-2960</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018736">DNA Adducts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008670">Metals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009942">Organometallic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C023502">copper bleomycin</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C023503">iron bleomycin</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C085742">peroxycobalt bleomycin</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C099533">zinc bleomycin</NameOfSubstance></Chemical><Chemical><RegistryNumber>11056-06-7</RegistryNumber><NameOfSubstance UI="D001761">Bleomycin</NameOfSubstance></Chemical><Chemical><RegistryNumber>3G0H8C9362</RegistryNumber><NameOfSubstance UI="D003035">Cobalt</NameOfSubstance></Chemical><Chemical><RegistryNumber>789U1901C5</RegistryNumber><NameOfSubstance UI="D003300">Copper</NameOfSubstance></Chemical><Chemical><RegistryNumber>E1UOL152H7</RegistryNumber><NameOfSubstance UI="D007501">Iron</NameOfSubstance></Chemical><Chemical><RegistryNumber>J41CSQ7QDS</RegistryNumber><NameOfSubstance UI="D015032">Zinc</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001665" MajorTopicYN="N">Binding Sites</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001761" MajorTopicYN="N">Bleomycin</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002942" MajorTopicYN="N">Circular Dichroism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003035" MajorTopicYN="N">Cobalt</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003300" MajorTopicYN="N">Copper</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018736" MajorTopicYN="N">DNA Adducts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007501" MajorTopicYN="N">Iron</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008670" MajorTopicYN="N">Metals</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019906" MajorTopicYN="N">Nuclear Magnetic Resonance, Biomolecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009942" MajorTopicYN="N">Organometallic Compounds</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013050" MajorTopicYN="N">Spectrometry, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015032" MajorTopicYN="N">Zinc</DescriptorName><QualifierName 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