Murine B-cell and T-cell epitopes of the allergen Hev b 5 from natural rubber latex.
Identifieur interne : 000021 ( Ncbi/Checkpoint ); précédent : 000020; suivant : 000022Murine B-cell and T-cell epitopes of the allergen Hev b 5 from natural rubber latex.
Auteurs : J E Slater [États-Unis] ; E J Paupore ; R E O'HehirSource :
- Molecular immunology [ 0161-5890 ] ; 1999.
Descripteurs français
- KwdFr :
- Allergènes (génétique), Allergènes (immunologie), Analyse de séquence, Animaux, Antigènes végétaux, Coopération des lymphocytes, Données de séquences moléculaires, Fragments peptidiques (génétique), Fragments peptidiques (immunologie), Hypersensibilité au latex (immunologie), Lymphocytes B (immunologie), Lymphocytes T (immunologie), Protéines végétales, Présentation d'antigène, Souris, Séquence d'acides aminés, Épitopes (génétique), Épitopes (immunologie).
- MESH :
- génétique : Allergènes, Fragments peptidiques, Épitopes.
- immunologie : Allergènes, Fragments peptidiques, Hypersensibilité au latex, Lymphocytes B, Lymphocytes T, Épitopes.
- Analyse de séquence, Animaux, Antigènes végétaux, Coopération des lymphocytes, Données de séquences moléculaires, Protéines végétales, Présentation d'antigène, Souris, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Allergens (genetics), Allergens (immunology), Amino Acid Sequence, Animals, Antigen Presentation, Antigens, Plant, B-Lymphocytes (immunology), Epitopes (genetics), Epitopes (immunology), Latex Hypersensitivity (immunology), Lymphocyte Cooperation, Mice, Molecular Sequence Data, Peptide Fragments (genetics), Peptide Fragments (immunology), Plant Proteins, Sequence Analysis, T-Lymphocytes (immunology).
- MESH :
- chemical , genetics : Allergens, Epitopes, Peptide Fragments.
- chemical , immunology : Allergens, Epitopes, Peptide Fragments.
- immunology : B-Lymphocytes, Latex Hypersensitivity, T-Lymphocytes.
- Amino Acid Sequence, Animals, Antigen Presentation, Antigens, Plant, Lymphocyte Cooperation, Mice, Molecular Sequence Data, Plant Proteins, Sequence Analysis.
Abstract
Hev b 5, a proline-rich acidic protein with a predominantly random secondary structure, is a major allergen in natural rubber latex and a candidate for specific immunotherapy of latex allergy. As a first step in the identification of candidate peptides for immunotherapy, we have begun to identify the B-cell and T-cell epitopes of Hev b 5 in BALB/c mice. The mice were immunized with a Hev b 5 fusion protein. The B-cell epitopes were determined by the SPOTS method using overlapping octamers or by ELISA inhibition using a series of overlapping 20-mers. The T-cell epitopes were determined by the proliferation and cytokine release of splenocytes cultured in the presence of the 20-mers. Potential antibody binding regions included residues in regions 1-38, 55-74, 109-128 and 132-151. Examination of the binding sequences for common motifs suggested enhanced antibody binding to the KXEE or KEXE sequences, where X is empty, threonine or alanine. Splenocyte stimulation and cytokine release suggest T-cell epitopes with the regions 1-20, 37-56, 73-101 and 109-146. Since they may contain major T-cell epitopes but do not exhibit significant antibody binding, peptide regions 38-48 and 75-101 are candidates for specific immunotherapy to Hev b 5 in the BALB/c mouse model.
DOI: 10.1016/s0161-5890(99)00021-8
PubMed: 10378685
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002628
- to stream PubMed, to step Curation: 002628
- to stream PubMed, to step Checkpoint: 002483
- to stream Ncbi, to step Merge: 000021
- to stream Ncbi, to step Curation: 000021
Links to Exploration step
pubmed:10378685Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Murine B-cell and T-cell epitopes of the allergen Hev b 5 from natural rubber latex.</title><author><name sortKey="Slater, J E" sort="Slater, J E" uniqKey="Slater J" first="J E" last="Slater">J E Slater</name><affiliation wicri:level="2"><nlm:affiliation>Center for Molecular Mechanisms of Disease Research, Children's Research Institute, Children's National Medical Center, Washington, DC 20010, USA. slaterj@cber.fda.gov</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Molecular Mechanisms of Disease Research, Children's Research Institute, Children's National Medical Center, Washington, DC 20010</wicri:regionArea><placeName><region type="state">District de Columbia</region></placeName></affiliation></author><author><name sortKey="Paupore, E J" sort="Paupore, E J" uniqKey="Paupore E" first="E J" last="Paupore">E J Paupore</name></author><author><name sortKey="O Hehir, R E" sort="O Hehir, R E" uniqKey="O Hehir R" first="R E" last="O'Hehir">R E O'Hehir</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:10378685</idno><idno type="pmid">10378685</idno><idno type="doi">10.1016/s0161-5890(99)00021-8</idno><idno type="wicri:Area/PubMed/Corpus">002628</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002628</idno><idno type="wicri:Area/PubMed/Curation">002628</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002628</idno><idno type="wicri:Area/PubMed/Checkpoint">002483</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002483</idno><idno type="wicri:Area/Ncbi/Merge">000021</idno><idno type="wicri:Area/Ncbi/Curation">000021</idno><idno type="wicri:Area/Ncbi/Checkpoint">000021</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Murine B-cell and T-cell epitopes of the allergen Hev b 5 from natural rubber latex.</title><author><name sortKey="Slater, J E" sort="Slater, J E" uniqKey="Slater J" first="J E" last="Slater">J E Slater</name><affiliation wicri:level="2"><nlm:affiliation>Center for Molecular Mechanisms of Disease Research, Children's Research Institute, Children's National Medical Center, Washington, DC 20010, USA. slaterj@cber.fda.gov</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Molecular Mechanisms of Disease Research, Children's Research Institute, Children's National Medical Center, Washington, DC 20010</wicri:regionArea><placeName><region type="state">District de Columbia</region></placeName></affiliation></author><author><name sortKey="Paupore, E J" sort="Paupore, E J" uniqKey="Paupore E" first="E J" last="Paupore">E J Paupore</name></author><author><name sortKey="O Hehir, R E" sort="O Hehir, R E" uniqKey="O Hehir R" first="R E" last="O'Hehir">R E O'Hehir</name></author></analytic><series><title level="j">Molecular immunology</title><idno type="ISSN">0161-5890</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Allergens (genetics)</term><term>Allergens (immunology)</term><term>Amino Acid Sequence</term><term>Animals</term><term>Antigen Presentation</term><term>Antigens, Plant</term><term>B-Lymphocytes (immunology)</term><term>Epitopes (genetics)</term><term>Epitopes (immunology)</term><term>Latex Hypersensitivity (immunology)</term><term>Lymphocyte Cooperation</term><term>Mice</term><term>Molecular Sequence Data</term><term>Peptide Fragments (genetics)</term><term>Peptide Fragments (immunology)</term><term>Plant Proteins</term><term>Sequence Analysis</term><term>T-Lymphocytes (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Allergènes (génétique)</term><term>Allergènes (immunologie)</term><term>Analyse de séquence</term><term>Animaux</term><term>Antigènes végétaux</term><term>Coopération des lymphocytes</term><term>Données de séquences moléculaires</term><term>Fragments peptidiques (génétique)</term><term>Fragments peptidiques (immunologie)</term><term>Hypersensibilité au latex (immunologie)</term><term>Lymphocytes B (immunologie)</term><term>Lymphocytes T (immunologie)</term><term>Protéines végétales</term><term>Présentation d'antigène</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Épitopes (génétique)</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Allergens</term><term>Epitopes</term><term>Peptide Fragments</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Allergens</term><term>Epitopes</term><term>Peptide Fragments</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Allergènes</term><term>Fragments peptidiques</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Allergènes</term><term>Fragments peptidiques</term><term>Hypersensibilité au latex</term><term>Lymphocytes B</term><term>Lymphocytes T</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>Latex Hypersensitivity</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antigen Presentation</term><term>Antigens, Plant</term><term>Lymphocyte Cooperation</term><term>Mice</term><term>Molecular Sequence Data</term><term>Plant Proteins</term><term>Sequence Analysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence</term><term>Animaux</term><term>Antigènes végétaux</term><term>Coopération des lymphocytes</term><term>Données de séquences moléculaires</term><term>Protéines végétales</term><term>Présentation d'antigène</term><term>Souris</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hev b 5, a proline-rich acidic protein with a predominantly random secondary structure, is a major allergen in natural rubber latex and a candidate for specific immunotherapy of latex allergy. As a first step in the identification of candidate peptides for immunotherapy, we have begun to identify the B-cell and T-cell epitopes of Hev b 5 in BALB/c mice. The mice were immunized with a Hev b 5 fusion protein. The B-cell epitopes were determined by the SPOTS method using overlapping octamers or by ELISA inhibition using a series of overlapping 20-mers. The T-cell epitopes were determined by the proliferation and cytokine release of splenocytes cultured in the presence of the 20-mers. Potential antibody binding regions included residues in regions 1-38, 55-74, 109-128 and 132-151. Examination of the binding sequences for common motifs suggested enhanced antibody binding to the KXEE or KEXE sequences, where X is empty, threonine or alanine. Splenocyte stimulation and cytokine release suggest T-cell epitopes with the regions 1-20, 37-56, 73-101 and 109-146. Since they may contain major T-cell epitopes but do not exhibit significant antibody binding, peptide regions 38-48 and 75-101 are candidates for specific immunotherapy to Hev b 5 in the BALB/c mouse model.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>District de Columbia</li></region></list><tree><noCountry><name sortKey="O Hehir, R E" sort="O Hehir, R E" uniqKey="O Hehir R" first="R E" last="O'Hehir">R E O'Hehir</name><name sortKey="Paupore, E J" sort="Paupore, E J" uniqKey="Paupore E" first="E J" last="Paupore">E J Paupore</name></noCountry><country name="États-Unis"><region name="District de Columbia"><name sortKey="Slater, J E" sort="Slater, J E" uniqKey="Slater J" first="J E" last="Slater">J E Slater</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000021 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000021 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:10378685
|texte= Murine B-cell and T-cell epitopes of the allergen Hev b 5 from natural rubber latex.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:10378685" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |