DNA-methylase from regenerating rat liver: purification and characterisation
Identifieur interne : 005099 ( Main/Merge ); précédent : 005098; suivant : 005100DNA-methylase from regenerating rat liver: purification and characterisation
Auteurs : D. Simon ; F. Grunert ; U. V. Acken ; H. P. Dörining ; H. KrögerSource :
- Nucleic Acids Research [ 0305-1048 ] ; 1978.
Abstract
DNA methylase has been purified 660-fold from nuclei from regenerating rat liver. The enzyme is able to methylate single stranded (ss) and double stranded (ds) DNA, the only reaction product being 5-methylcytosine. Previously unmethylated double stranded DNA from prokaryotes (M.luteus) as well as from euka- ryotes (Ascaris suis) can serve as substrates. The synthetic copolymers (dG-dC)n . (dC-dG)n and (dG,dC)n are also methylated. While SV40 DNA is almost not methylated, PM2 DNA is a good sub strate even in the supercoiled form. The enzyme methylates 1 in 17 bases in heterologous M.luteus DNA, but only 1 in 590 in homologous rat liver DNA. The high methylation level of M.hrteus DNA, an analysis of the methylated pyrimidine isostichs and a preliminary dinucleotide analysis suggest that all the CpGs in a DNA can be methylated.
Url:
DOI: 10.1093/nar/5.6.2153
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