Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA
Identifieur interne : 004B85 ( Main/Merge ); précédent : 004B84; suivant : 004B86Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA
Auteurs : Paul A. Feldstein [États-Unis] ; Jamal M. Buzayan [États-Unis] ; George Bruening [États-Unis]Source :
- Gene [ 0378-1119 ] ; 1989.
English descriptors
- Teeft :
- Autolytic, Autolytic processing, Bimolecular reaction, Bruening, Buzayan, Circle transcription, Cleavage, Cleavage reaction, Endoribonucleolytic, Endoribonucleolytic sequence, Gerlach, Haseloff, Junction phosphodiester bond, Multimeric, Multimeric forms, Nucleic, Nucleic acids, Nucleotide sequence, Phosphodiester, Phosphodiester bond, Plasmid, Replication, Ringspot, Rna, Rnase, Satellite tobacco ringspot virus, Schneider, Stobrv, Symons, Tobacco ringspot virus, Transcription, Transcription buffer, Virology.
Abstract
Abstract: Circular and multimeric forms of the satellite RNA of tobacco ringspot virus and their autolytic processing reactions are well known. They suggest replication models in which key elements are rolling circle transcription and the processing of the resulting multimeric RNA to generate the unit, ‘monomeric’ satellite RNA sequence. We prepared plasmids bearing two distinct sequences of the satellite RNA. Each was arranged to allow transcription of an oligoribonucleotide (r-oligo) of the polarity that is complementary to encapsidated satellite RNA. One sequence has the autolytic processing phosphodiester bond, ApG, and the other is located at a distance of about 150 nucleotide (nt) residues. The second r-oligo accomplished cleavage of the first, in a catalytic fashion. Analysis of truncated forms showed that 10 nt of the ApG junction-containing r-oligo and 46 of the endoribonucleolytic r-oligo were sufficient for recognition in the cleavage reaction. These results map the sequences involved in autolytic processing of the complementary polarity satellite RNA to two regions.
Url:
DOI: 10.1016/0378-1119(89)90029-2
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000928
- to stream Istex, to step Curation: 000928
- to stream Istex, to step Checkpoint: 002012
Links to Exploration step
ISTEX:693D4DBC004CDC12BE40F168185E2FC4D7429BDELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA</title>
<author><name sortKey="Feldstein, Paul A" sort="Feldstein, Paul A" uniqKey="Feldstein P" first="Paul A." last="Feldstein">Paul A. Feldstein</name>
</author>
<author><name sortKey="Buzayan, Jamal M" sort="Buzayan, Jamal M" uniqKey="Buzayan J" first="Jamal M." last="Buzayan">Jamal M. Buzayan</name>
</author>
<author><name sortKey="Bruening, George" sort="Bruening, George" uniqKey="Bruening G" first="George" last="Bruening">George Bruening</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:693D4DBC004CDC12BE40F168185E2FC4D7429BDE</idno>
<date when="1989" year="1989">1989</date>
<idno type="doi">10.1016/0378-1119(89)90029-2</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-WLNZ4X8H-V/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000928</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000928</idno>
<idno type="wicri:Area/Istex/Curation">000928</idno>
<idno type="wicri:Area/Istex/Checkpoint">002012</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002012</idno>
<idno type="wicri:doubleKey">0378-1119:1989:Feldstein P:two:sequences:participating</idno>
<idno type="wicri:Area/Main/Merge">004B85</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA</title>
<author><name sortKey="Feldstein, Paul A" sort="Feldstein, Paul A" uniqKey="Feldstein P" first="Paul A." last="Feldstein">Paul A. Feldstein</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Buzayan, Jamal M" sort="Buzayan, Jamal M" uniqKey="Buzayan J" first="Jamal M." last="Buzayan">Jamal M. Buzayan</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Bruening, George" sort="Bruening, George" uniqKey="Bruening G" first="George" last="Bruening">George Bruening</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Gene</title>
<title level="j" type="abbrev">GENE</title>
<idno type="ISSN">0378-1119</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1989">1989</date>
<biblScope unit="volume">82</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="53">53</biblScope>
<biblScope unit="page" to="61">61</biblScope>
</imprint>
<idno type="ISSN">0378-1119</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0378-1119</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Autolytic</term>
<term>Autolytic processing</term>
<term>Bimolecular reaction</term>
<term>Bruening</term>
<term>Buzayan</term>
<term>Circle transcription</term>
<term>Cleavage</term>
<term>Cleavage reaction</term>
<term>Endoribonucleolytic</term>
<term>Endoribonucleolytic sequence</term>
<term>Gerlach</term>
<term>Haseloff</term>
<term>Junction phosphodiester bond</term>
<term>Multimeric</term>
<term>Multimeric forms</term>
<term>Nucleic</term>
<term>Nucleic acids</term>
<term>Nucleotide sequence</term>
<term>Phosphodiester</term>
<term>Phosphodiester bond</term>
<term>Plasmid</term>
<term>Replication</term>
<term>Ringspot</term>
<term>Rna</term>
<term>Rnase</term>
<term>Satellite tobacco ringspot virus</term>
<term>Schneider</term>
<term>Stobrv</term>
<term>Symons</term>
<term>Tobacco ringspot virus</term>
<term>Transcription</term>
<term>Transcription buffer</term>
<term>Virology</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Circular and multimeric forms of the satellite RNA of tobacco ringspot virus and their autolytic processing reactions are well known. They suggest replication models in which key elements are rolling circle transcription and the processing of the resulting multimeric RNA to generate the unit, ‘monomeric’ satellite RNA sequence. We prepared plasmids bearing two distinct sequences of the satellite RNA. Each was arranged to allow transcription of an oligoribonucleotide (r-oligo) of the polarity that is complementary to encapsidated satellite RNA. One sequence has the autolytic processing phosphodiester bond, ApG, and the other is located at a distance of about 150 nucleotide (nt) residues. The second r-oligo accomplished cleavage of the first, in a catalytic fashion. Analysis of truncated forms showed that 10 nt of the ApG junction-containing r-oligo and 46 of the endoribonucleolytic r-oligo were sufficient for recognition in the cleavage reaction. These results map the sequences involved in autolytic processing of the complementary polarity satellite RNA to two regions.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004B85 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004B85 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Merge |type= RBID |clé= ISTEX:693D4DBC004CDC12BE40F168185E2FC4D7429BDE |texte= Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA }}
This area was generated with Dilib version V0.6.33. |