Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA
Identifieur interne : 000928 ( Istex/Corpus ); précédent : 000927; suivant : 000929Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA
Auteurs : Paul A. Feldstein ; Jamal M. Buzayan ; George BrueningSource :
- Gene [ 0378-1119 ] ; 1989.
English descriptors
- Teeft :
- Autolytic, Autolytic processing, Bimolecular reaction, Bruening, Buzayan, Circle transcription, Cleavage, Cleavage reaction, Endoribonucleolytic, Endoribonucleolytic sequence, Gerlach, Haseloff, Junction phosphodiester bond, Multimeric, Multimeric forms, Nucleic, Nucleic acids, Nucleotide sequence, Phosphodiester, Phosphodiester bond, Plasmid, Replication, Ringspot, Rna, Rnase, Satellite tobacco ringspot virus, Schneider, Stobrv, Symons, Tobacco ringspot virus, Transcription, Transcription buffer, Virology.
Abstract
Abstract: Circular and multimeric forms of the satellite RNA of tobacco ringspot virus and their autolytic processing reactions are well known. They suggest replication models in which key elements are rolling circle transcription and the processing of the resulting multimeric RNA to generate the unit, ‘monomeric’ satellite RNA sequence. We prepared plasmids bearing two distinct sequences of the satellite RNA. Each was arranged to allow transcription of an oligoribonucleotide (r-oligo) of the polarity that is complementary to encapsidated satellite RNA. One sequence has the autolytic processing phosphodiester bond, ApG, and the other is located at a distance of about 150 nucleotide (nt) residues. The second r-oligo accomplished cleavage of the first, in a catalytic fashion. Analysis of truncated forms showed that 10 nt of the ApG junction-containing r-oligo and 46 of the endoribonucleolytic r-oligo were sufficient for recognition in the cleavage reaction. These results map the sequences involved in autolytic processing of the complementary polarity satellite RNA to two regions.
Url:
DOI: 10.1016/0378-1119(89)90029-2
Links to Exploration step
ISTEX:693D4DBC004CDC12BE40F168185E2FC4D7429BDELe document en format XML
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(F.A</mods:affiliation></affiliation></author><author><name sortKey="Bruening, George" sort="Bruening, George" uniqKey="Bruening G" first="George" last="Bruening">George Bruening</name><affiliation><mods:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:693D4DBC004CDC12BE40F168185E2FC4D7429BDE</idno><date when="1989" year="1989">1989</date><idno type="doi">10.1016/0378-1119(89)90029-2</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-WLNZ4X8H-V/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000928</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000928</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA</title><author><name sortKey="Feldstein, Paul A" sort="Feldstein, Paul A" uniqKey="Feldstein P" first="Paul A." last="Feldstein">Paul A. Feldstein</name><affiliation><mods:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</mods:affiliation></affiliation></author><author><name sortKey="Buzayan, Jamal M" sort="Buzayan, Jamal M" uniqKey="Buzayan J" first="Jamal M." last="Buzayan">Jamal M. Buzayan</name><affiliation><mods:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</mods:affiliation></affiliation></author><author><name sortKey="Bruening, George" sort="Bruening, George" uniqKey="Bruening G" first="George" last="Bruening">George Bruening</name><affiliation><mods:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="ISSN">0378-1119</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">82</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="53">53</biblScope><biblScope unit="page" to="61">61</biblScope></imprint><idno type="ISSN">0378-1119</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1119</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Autolytic</term><term>Autolytic processing</term><term>Bimolecular reaction</term><term>Bruening</term><term>Buzayan</term><term>Circle transcription</term><term>Cleavage</term><term>Cleavage reaction</term><term>Endoribonucleolytic</term><term>Endoribonucleolytic sequence</term><term>Gerlach</term><term>Haseloff</term><term>Junction phosphodiester bond</term><term>Multimeric</term><term>Multimeric forms</term><term>Nucleic</term><term>Nucleic acids</term><term>Nucleotide sequence</term><term>Phosphodiester</term><term>Phosphodiester bond</term><term>Plasmid</term><term>Replication</term><term>Ringspot</term><term>Rna</term><term>Rnase</term><term>Satellite tobacco ringspot virus</term><term>Schneider</term><term>Stobrv</term><term>Symons</term><term>Tobacco ringspot virus</term><term>Transcription</term><term>Transcription buffer</term><term>Virology</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Circular and multimeric forms of the satellite RNA of tobacco ringspot virus and their autolytic processing reactions are well known. They suggest replication models in which key elements are rolling circle transcription and the processing of the resulting multimeric RNA to generate the unit, ‘monomeric’ satellite RNA sequence. We prepared plasmids bearing two distinct sequences of the satellite RNA. Each was arranged to allow transcription of an oligoribonucleotide (r-oligo) of the polarity that is complementary to encapsidated satellite RNA. One sequence has the autolytic processing phosphodiester bond, ApG, and the other is located at a distance of about 150 nucleotide (nt) residues. The second r-oligo accomplished cleavage of the first, in a catalytic fashion. Analysis of truncated forms showed that 10 nt of the ApG junction-containing r-oligo and 46 of the endoribonucleolytic r-oligo were sufficient for recognition in the cleavage reaction. These results map the sequences involved in autolytic processing of the complementary polarity satellite RNA to two regions.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>stobrv</json:string><json:string>buzayan</json:string><json:string>autolytic</json:string><json:string>gerlach</json:string><json:string>plasmid</json:string><json:string>bruening</json:string><json:string>haseloff</json:string><json:string>ringspot</json:string><json:string>phosphodiester</json:string><json:string>symons</json:string><json:string>autolytic processing</json:string><json:string>endoribonucleolytic</json:string><json:string>rna</json:string><json:string>schneider</json:string><json:string>virology</json:string><json:string>rnase</json:string><json:string>nucleic acids</json:string><json:string>multimeric</json:string><json:string>endoribonucleolytic sequence</json:string><json:string>satellite tobacco ringspot virus</json:string><json:string>cleavage</json:string><json:string>multimeric forms</json:string><json:string>phosphodiester bond</json:string><json:string>bimolecular reaction</json:string><json:string>replication</json:string><json:string>nucleic</json:string><json:string>circle transcription</json:string><json:string>cleavage reaction</json:string><json:string>tobacco ringspot virus</json:string><json:string>nucleotide sequence</json:string><json:string>junction phosphodiester bond</json:string><json:string>transcription buffer</json:string><json:string>transcription</json:string></teeft></keywords><author><json:item><name>Paul A. Feldstein</name><affiliations><json:string>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</json:string></affiliations></json:item><json:item><name>Jamal M. Buzayan</name><affiliations><json:string>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</json:string></affiliations></json:item><json:item><name>George Bruening</name><affiliations><json:string>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Catalytic RNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>endonucleolytic RNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>plant virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>plasmid vector</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>recombinant DNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RNA self-cleavage</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RNA enzyme</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>rolling circle transcription</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>D : promoter distal bordering</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>M : full monomeric</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>P : promoter proximal bordering</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>P-M-D : processing phosphodiester bonds</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>d-oligo : oligodeoxyribonucleotide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DTT : dithiothreitol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>nt : nucleotide(s)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Pollk : Klenow (large) fragment of E. coli DNA polymerase I</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>r-oligo : oligoribonucleotide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>STobRV ( + )RNA : satellite TobRV RNA of the polarity that is most abundantly encapsidated</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>STobRV (-)RNA : RNA complementary to STobRV ( + )RNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>TobRV : tobacco ringspot virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>TM : transcription buffers</value></json:item></subject><arkIstex>ark:/67375/6H6-WLNZ4X8H-V</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Circular and multimeric forms of the satellite RNA of tobacco ringspot virus and their autolytic processing reactions are well known. They suggest replication models in which key elements are rolling circle transcription and the processing of the resulting multimeric RNA to generate the unit, ‘monomeric’ satellite RNA sequence. We prepared plasmids bearing two distinct sequences of the satellite RNA. Each was arranged to allow transcription of an oligoribonucleotide (r-oligo) of the polarity that is complementary to encapsidated satellite RNA. One sequence has the autolytic processing phosphodiester bond, ApG, and the other is located at a distance of about 150 nucleotide (nt) residues. The second r-oligo accomplished cleavage of the first, in a catalytic fashion. Analysis of truncated forms showed that 10 nt of the ApG junction-containing r-oligo and 46 of the endoribonucleolytic r-oligo were sufficient for recognition in the cleavage reaction. These results map the sequences involved in autolytic processing of the complementary polarity satellite RNA to two regions.</abstract><qualityIndicators><score>7.89</score><pdfWordCount>3970</pdfWordCount><pdfCharCount>26698</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>540 x 756 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>160</abstractWordCount><abstractCharCount>1094</abstractCharCount><keywordCount>21</keywordCount></qualityIndicators><title>Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA</title><pmid><json:string>2583519</json:string></pmid><pii><json:string>0378-1119(89)90029-2</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. 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Gerlach</json:string><json:string>G. Gough</json:string><json:string>College</json:string><json:string>Results</json:string><json:string>G. Bruening</json:string><json:string>Experiment Station</json:string><json:string>H. Partial</json:string><json:string>James Haseloff</json:string><json:string>J. Haseloff</json:string><json:string>Wayne L. Gerlach</json:string><json:string>U.S. Biochemicals</json:string><json:string>P.T. Gilham</json:string><json:string>Jamal M. Buzayan</json:string></persName><placeName><json:string>Albany</json:string><json:string>Mannheim</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Forster and Symons, 1987a</json:string><json:string>Buzayan et al., 1986</json:string><json:string>Sogo and Schneider, 1982</json:string><json:string>Gerlach et al., 1986</json:string><json:string>Haseloff and Symons, 1981</json:string><json:string>Bruening et al., 1988</json:string><json:string>Buzayan et al., 1986b</json:string><json:string>Richardson, 1981</json:string><json:string>Donis-Keller et al., 1977</json:string><json:string>Kiefer et al., 1982</json:string><json:string>Ponz et al., 1987</json:string><json:string>Birnboim and Doly (1979)</json:string><json:string>Buzayan et al., 1986a</json:string><json:string>DonisKeller, 1979</json:string><json:string>Davanloo et al., 1984</json:string><json:string>Buzayan et al</json:string><json:string>Buzayan et al., 1988</json:string><json:string>Schneider, 1977</json:string><json:string>Haselo~~d Gerlach, 1988</json:string><json:string>Hutchins et al., 1986</json:string><json:string>Maniatis et al., 1982</json:string><json:string>Symons et al., 1985</json:string><json:string>Koizumi et al., 1988</json:string><json:string>Prody et al., 1986</json:string><json:string>England et al., 1980</json:string><json:string>Bock, 1967</json:string><json:string>Haseloff and Gerlach (1989)</json:string><json:string>Forster and Symons, 1987b</json:string><json:string>Buzayan et al., 1987</json:string><json:string>Haseloff and Gerlach, 1989</json:string><json:string>Linthorst and Kaper, 1984</json:string><json:string>Uhienbeck, 1987</json:string><json:string>Buzayan et al., 1986c, 1988</json:string><json:string>Gerlach et al., 1987</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-WLNZ4X8H-V</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - genetics & heredity</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - developmental biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Genetics</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - biologie moleculaire et cellulaire</json:string></inist></categories><publicationDate>1989</publicationDate><copyrightDate>1989</copyrightDate><doi><json:string>10.1016/0378-1119(89)90029-2</json:string></doi><id>693D4DBC004CDC12BE40F168185E2FC4D7429BDE</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-WLNZ4X8H-V/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-WLNZ4X8H-V/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-WLNZ4X8H-V/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1989</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>Presented at the Albany Conference on ‘RNA: Catalysis, Splicing, Evolution’, Rensselaerville, NY (U.S.A.) 22–25 September 1988.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA</title><author xml:id="author-0000"><persName><forename type="first">Paul A.</forename><surname>Feldstein</surname></persName><affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Jamal M.</forename><surname>Buzayan</surname></persName><affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</affiliation></author><author xml:id="author-0002"><persName><forename type="first">George</forename><surname>Bruening</surname></persName><note type="correspondence"><p>Correspondence to: Dr. G. Bruening, Plant Pathology, University of California, Davis, CA 95616, Tel.(916)752-3474; Fax (916) 752-5674.</p></note><affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</affiliation></author><idno type="istex">693D4DBC004CDC12BE40F168185E2FC4D7429BDE</idno><idno type="ark">ark:/67375/6H6-WLNZ4X8H-V</idno><idno type="DOI">10.1016/0378-1119(89)90029-2</idno><idno type="PII">0378-1119(89)90029-2</idno></analytic><monogr><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="pISSN">0378-1119</idno><idno type="PII">S0378-1119(00)X0421-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989"></date><biblScope unit="volume">82</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="53">53</biblScope><biblScope unit="page" to="61">61</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1989</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Circular and multimeric forms of the satellite RNA of tobacco ringspot virus and their autolytic processing reactions are well known. They suggest replication models in which key elements are rolling circle transcription and the processing of the resulting multimeric RNA to generate the unit, ‘monomeric’ satellite RNA sequence. We prepared plasmids bearing two distinct sequences of the satellite RNA. Each was arranged to allow transcription of an oligoribonucleotide (r-oligo) of the polarity that is complementary to encapsidated satellite RNA. One sequence has the autolytic processing phosphodiester bond, ApG, and the other is located at a distance of about 150 nucleotide (nt) residues. The second r-oligo accomplished cleavage of the first, in a catalytic fashion. Analysis of truncated forms showed that 10 nt of the ApG junction-containing r-oligo and 46 of the endoribonucleolytic r-oligo were sufficient for recognition in the cleavage reaction. These results map the sequences involved in autolytic processing of the complementary polarity satellite RNA to two regions.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Catalytic RNA</term></item><item><term>endonucleolytic RNA</term></item><item><term>plant virus</term></item><item><term>plasmid vector</term></item><item><term>recombinant DNA</term></item><item><term>RNA self-cleavage</term></item><item><term>RNA enzyme</term></item><item><term>rolling circle transcription</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>D</term><term>promoter distal bordering</term></item><item><term>M</term><term>full monomeric</term></item><item><term>P</term><term>promoter proximal bordering</term></item><item><term>P-M-D</term><term>processing phosphodiester bonds</term></item><item><term>d-oligo</term><term>oligodeoxyribonucleotide</term></item><item><term>DTT</term><term>dithiothreitol</term></item><item><term>nt</term><term>nucleotide(s)</term></item><item><term>Pollk</term><term>Klenow (large) fragment of E. coli DNA polymerase I</term></item><item><term>r-oligo</term><term>oligoribonucleotide</term></item><item><term>STobRV ( + )RNA</term><term>satellite TobRV RNA of the polarity that is most abundantly encapsidated</term></item><item><term>STobRV (-)RNA</term><term>RNA complementary to STobRV ( + )RNA</term></item><item><term>TobRV</term><term>tobacco ringspot virus</term></item><item><term>TM</term><term>transcription buffers</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1989">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-WLNZ4X8H-V/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>GENE</jid><aid>89900292</aid><ce:pii>0378-1119(89)90029-2</ce:pii><ce:doi>10.1016/0378-1119(89)90029-2</ce:doi><ce:copyright type="unknown" year="1989"></ce:copyright></item-info><head><ce:article-footnote><ce:label>☆</ce:label><ce:note-para>Presented at the Albany Conference on ‘RNA: Catalysis, Splicing, Evolution’, Rensselaerville, NY (U.S.A.) 22–25 September 1988.</ce:note-para></ce:article-footnote><ce:title>Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA</ce:title><ce:author-group><ce:author><ce:given-name>Paul A.</ce:given-name><ce:surname>Feldstein</ce:surname></ce:author><ce:author><ce:given-name>Jamal M.</ce:given-name><ce:surname>Buzayan</ce:surname></ce:author><ce:author><ce:given-name>George</ce:given-name><ce:surname>Bruening</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: Dr. G. Bruening, Plant Pathology, University of California, Davis, CA 95616, Tel.(916)752-3474; Fax (916) 752-5674.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="6" month="2" year="1989"></ce:date-received><ce:date-accepted day="20" month="3" year="1989"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Circular and multimeric forms of the satellite RNA of tobacco ringspot virus and their autolytic processing reactions are well known. They suggest replication models in which key elements are rolling circle transcription and the processing of the resulting multimeric RNA to generate the unit, ‘monomeric’ satellite RNA sequence. We prepared plasmids bearing two distinct sequences of the satellite RNA. Each was arranged to allow transcription of an oligoribonucleotide (r-oligo) of the polarity that is complementary to encapsidated satellite RNA. One sequence has the autolytic processing phosphodiester bond, ApG, and the other is located at a distance of about 150 nucleotide (nt) residues. The second r-oligo accomplished cleavage of the first, in a catalytic fashion. Analysis of truncated forms showed that 10 nt of the ApG junction-containing r-oligo and 46 of the endoribonucleolytic r-oligo were sufficient for recognition in the cleavage reaction. These results map the sequences involved in autolytic processing of the complementary polarity satellite RNA to two regions.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Catalytic RNA</ce:text></ce:keyword><ce:keyword><ce:text>endonucleolytic RNA</ce:text></ce:keyword><ce:keyword><ce:text>plant virus</ce:text></ce:keyword><ce:keyword><ce:text>plasmid vector</ce:text></ce:keyword><ce:keyword><ce:text>recombinant DNA</ce:text></ce:keyword><ce:keyword><ce:text>RNA self-cleavage</ce:text></ce:keyword><ce:keyword><ce:text>RNA enzyme</ce:text></ce:keyword><ce:keyword><ce:text>rolling circle transcription</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>D</ce:text><ce:keyword><ce:text>promoter distal bordering</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>M</ce:text><ce:keyword><ce:text>full monomeric</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>P</ce:text><ce:keyword><ce:text>promoter proximal bordering</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>P-M-D</ce:text><ce:keyword><ce:text>processing phosphodiester bonds</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>d-oligo</ce:text><ce:keyword><ce:text>oligodeoxyribonucleotide</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>DTT</ce:text><ce:keyword><ce:text>dithiothreitol</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>nt</ce:text><ce:keyword><ce:text>nucleotide(s)</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>Pollk</ce:text><ce:keyword><ce:text>Klenow (large) fragment of <ce:italic>E. coli</ce:italic> DNA polymerase I</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>r-oligo</ce:text><ce:keyword><ce:text>oligoribonucleotide</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>STobRV ( + )RNA</ce:text><ce:keyword><ce:text>satellite TobRV RNA of the polarity that is most abundantly encapsidated</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>STobRV (-)RNA</ce:text><ce:keyword><ce:text>RNA complementary to STobRV ( + )RNA</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>TobRV</ce:text><ce:keyword><ce:text>tobacco ringspot virus</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>TM</ce:text><ce:keyword><ce:text>transcription buffers</ce:text></ce:keyword></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Two sequences participating in the autolytic processing of satellite tobacco ringspot virus comple- mentary RNA</title></titleInfo><name type="personal"><namePart type="given">Paul A.</namePart><namePart type="family">Feldstein</namePart><affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jamal M.</namePart><namePart type="family">Buzayan</namePart><affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">George</namePart><namePart type="family">Bruening</namePart><affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616 U.S.A. Tel. (F.A</affiliation><description>Correspondence to: Dr. G. Bruening, Plant Pathology, University of California, Davis, CA 95616, Tel.(916)752-3474; Fax (916) 752-5674.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Circular and multimeric forms of the satellite RNA of tobacco ringspot virus and their autolytic processing reactions are well known. They suggest replication models in which key elements are rolling circle transcription and the processing of the resulting multimeric RNA to generate the unit, ‘monomeric’ satellite RNA sequence. We prepared plasmids bearing two distinct sequences of the satellite RNA. Each was arranged to allow transcription of an oligoribonucleotide (r-oligo) of the polarity that is complementary to encapsidated satellite RNA. One sequence has the autolytic processing phosphodiester bond, ApG, and the other is located at a distance of about 150 nucleotide (nt) residues. The second r-oligo accomplished cleavage of the first, in a catalytic fashion. Analysis of truncated forms showed that 10 nt of the ApG junction-containing r-oligo and 46 of the endoribonucleolytic r-oligo were sufficient for recognition in the cleavage reaction. These results map the sequences involved in autolytic processing of the complementary polarity satellite RNA to two regions.</abstract><note>Presented at the Albany Conference on ‘RNA: Catalysis, Splicing, Evolution’, Rensselaerville, NY (U.S.A.) 22–25 September 1988.</note><subject><genre>Keywords</genre><topic>Catalytic RNA</topic><topic>endonucleolytic RNA</topic><topic>plant virus</topic><topic>plasmid vector</topic><topic>recombinant DNA</topic><topic>RNA self-cleavage</topic><topic>RNA enzyme</topic><topic>rolling circle transcription</topic></subject><subject><genre>Abbreviations</genre><topic>D : promoter distal bordering</topic><topic>M : full monomeric</topic><topic>P : promoter proximal bordering</topic><topic>P-M-D : processing phosphodiester bonds</topic><topic>d-oligo : oligodeoxyribonucleotide</topic><topic>DTT : dithiothreitol</topic><topic>nt : nucleotide(s)</topic><topic>Pollk : Klenow (large) fragment of E. coli DNA polymerase I</topic><topic>r-oligo : oligoribonucleotide</topic><topic>STobRV ( + )RNA : satellite TobRV RNA of the polarity that is most abundantly encapsidated</topic><topic>STobRV (-)RNA : RNA complementary to STobRV ( + )RNA</topic><topic>TobRV : tobacco ringspot virus</topic><topic>TM : transcription buffers</topic></subject><relatedItem type="host"><titleInfo><title>Gene</title></titleInfo><titleInfo type="abbreviated"><title>GENE</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued></originInfo><identifier type="ISSN">0378-1119</identifier><identifier type="PII">S0378-1119(00)X0421-0</identifier><part><date>1989</date><detail type="volume"><number>82</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>189</end></extent><extent unit="pages"><start>53</start><end>61</end></extent></part></relatedItem><identifier type="istex">693D4DBC004CDC12BE40F168185E2FC4D7429BDE</identifier><identifier type="ark">ark:/67375/6H6-WLNZ4X8H-V</identifier><identifier type="DOI">10.1016/0378-1119(89)90029-2</identifier><identifier type="PII">0378-1119(89)90029-2</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-WLNZ4X8H-V/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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