RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA.
Identifieur interne : 004788 ( Main/Merge ); précédent : 004787; suivant : 004789RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA.
Auteurs : S. Mazurier [Pays-Bas] ; A. Van De Giessen ; K. Heuvelman ; K. WernarsSource :
- Letters in applied microbiology [ 0266-8254 ] ; 1992.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN bactérien.
- génétique : Campylobacter jejuni.
- isolement et purification : Campylobacter jejuni.
- ADN simple brin, Données de séquences moléculaires, Profilage d'ADN, Séquence nucléotidique, Techniques d'amplification d'acides nucléiques.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Bacterial.
- genetics : Campylobacter jejuni.
- isolation & purification : Campylobacter jejuni.
- methods : DNA Fingerprinting.
- Base Sequence, DNA, Single-Stranded, Molecular Sequence Data, Nucleic Acid Amplification Techniques.
Abstract
A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.
DOI: 10.1111/j.1472-765x.1992.tb00700.x
PubMed: 1368370
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pubmed:1368370Le document en format XML
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<term>Campylobacter jejuni (isolation & purification)</term>
<term>DNA Fingerprinting (methods)</term>
<term>DNA, Bacterial (analysis)</term>
<term>DNA, Single-Stranded</term>
<term>Molecular Sequence Data</term>
<term>Nucleic Acid Amplification Techniques</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (analyse)</term>
<term>ADN simple brin</term>
<term>Campylobacter jejuni (génétique)</term>
<term>Campylobacter jejuni (isolement et purification)</term>
<term>Données de séquences moléculaires</term>
<term>Profilage d'ADN ()</term>
<term>Séquence nucléotidique</term>
<term>Techniques d'amplification d'acides nucléiques</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term>
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<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN bactérien</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Campylobacter jejuni</term>
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<term>Molecular Sequence Data</term>
<term>Nucleic Acid Amplification Techniques</term>
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<keywords scheme="MESH" xml:lang="fr"><term>ADN simple brin</term>
<term>Données de séquences moléculaires</term>
<term>Profilage d'ADN</term>
<term>Séquence nucléotidique</term>
<term>Techniques d'amplification d'acides nucléiques</term>
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<front><div type="abstract" xml:lang="en">A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.</div>
</front>
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