RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA.
Identifieur interne : 000219 ( Ncbi/Merge ); précédent : 000218; suivant : 000220RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA.
Auteurs : S. Mazurier [Pays-Bas] ; A. Van De Giessen ; K. Heuvelman ; K. WernarsSource :
- Letters in applied microbiology [ 0266-8254 ] ; 1992.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN bactérien.
- génétique : Campylobacter jejuni.
- isolement et purification : Campylobacter jejuni.
- ADN simple brin, Données de séquences moléculaires, Profilage d'ADN, Séquence nucléotidique, Techniques d'amplification d'acides nucléiques.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Bacterial.
- genetics : Campylobacter jejuni.
- isolation & purification : Campylobacter jejuni.
- methods : DNA Fingerprinting.
- Base Sequence, DNA, Single-Stranded, Molecular Sequence Data, Nucleic Acid Amplification Techniques.
Abstract
A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.
DOI: 10.1111/j.1472-765x.1992.tb00700.x
PubMed: 1368370
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pubmed:1368370Le document en format XML
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Mazurier</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Van De Giessen, A" sort="Van De Giessen, A" uniqKey="Van De Giessen A" first="A" last="Van De Giessen">A. Van De Giessen</name></author><author><name sortKey="Heuvelman, K" sort="Heuvelman, K" uniqKey="Heuvelman K" first="K" last="Heuvelman">K. Heuvelman</name></author><author><name sortKey="Wernars, K" sort="Wernars, K" uniqKey="Wernars K" first="K" last="Wernars">K. Wernars</name></author></analytic><series><title level="j">Letters in applied microbiology</title><idno type="ISSN">0266-8254</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Campylobacter jejuni (genetics)</term><term>Campylobacter jejuni (isolation & purification)</term><term>DNA Fingerprinting (methods)</term><term>DNA, Bacterial (analysis)</term><term>DNA, Single-Stranded</term><term>Molecular Sequence Data</term><term>Nucleic Acid Amplification Techniques</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (analyse)</term><term>ADN simple brin</term><term>Campylobacter jejuni (génétique)</term><term>Campylobacter jejuni (isolement et purification)</term><term>Données de séquences moléculaires</term><term>Profilage d'ADN ()</term><term>Séquence nucléotidique</term><term>Techniques d'amplification d'acides nucléiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN bactérien</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Campylobacter jejuni</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Campylobacter jejuni</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Campylobacter jejuni</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Campylobacter jejuni</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>DNA Fingerprinting</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>DNA, Single-Stranded</term><term>Molecular Sequence Data</term><term>Nucleic Acid Amplification Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN simple brin</term><term>Données de séquences moléculaires</term><term>Profilage d'ADN</term><term>Séquence nucléotidique</term><term>Techniques d'amplification d'acides nucléiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1368370</PMID><DateCompleted><Year>1992</Year><Month>08</Month><Day>31</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>11</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0266-8254</ISSN><JournalIssue CitedMedium="Print"><Volume>14</Volume><Issue>6</Issue><PubDate><Year>1992</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Letters in applied microbiology</Title><ISOAbbreviation>Lett. Appl. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA.</ArticleTitle><Pagination><MedlinePgn>260-2</MedlinePgn></Pagination><Abstract><AbstractText>A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mazurier</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van de Giessen</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Heuvelman</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Wernars</LastName><ForeName>K</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Lett Appl Microbiol</MedlineTA><NlmUniqueID>8510094</NlmUniqueID><ISSNLinking>0266-8254</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004277">DNA, Single-Stranded</NameOfSubstance></Chemical></ChemicalList><CitationSubset>B</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016123" MajorTopicYN="N">Campylobacter jejuni</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016172" MajorTopicYN="N">DNA Fingerprinting</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004277" MajorTopicYN="N">DNA, Single-Stranded</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021141" MajorTopicYN="Y">Nucleic Acid Amplification Techniques</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>6</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1368370</ArticleId><ArticleId IdType="doi">10.1111/j.1472-765x.1992.tb00700.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country></list><tree><noCountry><name sortKey="Heuvelman, K" sort="Heuvelman, K" uniqKey="Heuvelman K" first="K" last="Heuvelman">K. Heuvelman</name><name sortKey="Van De Giessen, A" sort="Van De Giessen, A" uniqKey="Van De Giessen A" first="A" last="Van De Giessen">A. Van De Giessen</name><name sortKey="Wernars, K" sort="Wernars, K" uniqKey="Wernars K" first="K" last="Wernars">K. Wernars</name></noCountry><country name="Pays-Bas"><noRegion><name sortKey="Mazurier, S" sort="Mazurier, S" uniqKey="Mazurier S" first="S" last="Mazurier">S. Mazurier</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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