Incomplete replication of human parainfluenza virus type 4 in LLC-MK2 cells and in L929 cells
Identifieur interne : 003775 ( Main/Merge ); précédent : 003774; suivant : 003776Incomplete replication of human parainfluenza virus type 4 in LLC-MK2 cells and in L929 cells
Auteurs : Hiroshi Komada [Japon] ; Hisae Inoue [Japon] ; Chihiro Yamabayashi [Japon] ; Morihiro Ito [Japon] ; Mitsuo Kawano [Japon] ; Machiko Nishio [Japon] ; Masato Tsurudome [Japon] ; Yuji Kozuka [Japon] ; Naoya Noda [Japon] ; Kazuyoshi Namba [Japon] ; Myles O'Brien [Japon] ; Yasuhiko Ito [Japon]Source :
- Medical Microbiology and Immunology [ 0300-8584 ] ; 2000-06-01.
English descriptors
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Abstract
Abstract: Human parainfluenza virus type 4A (hPIV-4A) and type 4B (hPIV-4B) were tested for their ability to replicate in the monkey kidney LLC-MK2 cell line (MK2 cells) and the murine L929 cell line (L929 cells). These cells are normally non-permissive for replication of hPIV-4; however, treatment with acetylated trypsin led to virus replication in MK2 cells, but was less effective for L929 cells. Endogenously produced interferon (IFN) played no role in virus replication in L929 cells. Synthesis of virus-specific polypeptides was suppressed in L929 cells. WhereasNP-mRNA and HN-mRNA were detected in MK2 cells, no HN-mRNA was detected in L929 cells. These results indicate that hPIV-4 can infect both MK2 cells and L929 cells. In MK2 cells, when protease exists in the extracellular medium, hPIV-4 exhibits multistep growth. In L929 cells, however, the cause of incomplete replication might be lack of other unknown factors.
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DOI: 10.1007/s004300000037
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ISTEX:ED266053F87B394B8DBB70DB63B7CB6860247878Le document en format XML
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<front><div type="abstract" xml:lang="en">Abstract: Human parainfluenza virus type 4A (hPIV-4A) and type 4B (hPIV-4B) were tested for their ability to replicate in the monkey kidney LLC-MK2 cell line (MK2 cells) and the murine L929 cell line (L929 cells). These cells are normally non-permissive for replication of hPIV-4; however, treatment with acetylated trypsin led to virus replication in MK2 cells, but was less effective for L929 cells. Endogenously produced interferon (IFN) played no role in virus replication in L929 cells. Synthesis of virus-specific polypeptides was suppressed in L929 cells. WhereasNP-mRNA and HN-mRNA were detected in MK2 cells, no HN-mRNA was detected in L929 cells. These results indicate that hPIV-4 can infect both MK2 cells and L929 cells. In MK2 cells, when protease exists in the extracellular medium, hPIV-4 exhibits multistep growth. In L929 cells, however, the cause of incomplete replication might be lack of other unknown factors.</div>
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