Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum
Identifieur interne : 003091 ( Main/Merge ); précédent : 003090; suivant : 003092Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum
Auteurs : Michael Alifrangis [Danemark] ; Inge T. Christensen [Danemark] ; Flemming S. J Rgensen [Danemark] ; Anita M. R Nn [Danemark] ; Jimmy E. Weng [Danemark] ; Ming Chen [Danemark] ; Ib C. Bygbjerg [Danemark] ; Worachart Sirawaraporn [Thaïlande] ; Yaseelan Palarasah [Danemark] ; Claus Koch [Danemark]Source :
- Malaria Journal [ 1475-2875 ] ; 2004.
Descripteurs français
- KwdFr :
- Acides aminés (), Animaux, Anticorps antiprotozoaires (immunologie), Anticorps antiprotozoaires (sang), Anticorps monoclonaux (immunologie), Antigènes de protozoaire (), Antigènes de protozoaire (immunologie), Cartographie épitopique, Dihydrofolate reductase (), Dihydrofolate reductase (immunologie), Humains, Lapins, Modèles moléculaires, Plasmodium falciparum (enzymologie), Plasmodium falciparum (immunologie), Souris, Sérums immuns (immunologie), Technique de Western, Test ELISA, Électrophorèse sur gel de polyacrylamide, Épitopes (), Épitopes (analyse), Épitopes (immunologie).
- MESH :
- analyse : Épitopes.
- enzymologie : Plasmodium falciparum.
- immunologie : Anticorps antiprotozoaires, Anticorps monoclonaux, Antigènes de protozoaire, Dihydrofolate reductase, Plasmodium falciparum, Sérums immuns, Épitopes.
- sang : Anticorps antiprotozoaires.
- Acides aminés, Animaux, Antigènes de protozoaire, Cartographie épitopique, Dihydrofolate reductase, Humains, Lapins, Modèles moléculaires, Souris, Technique de Western, Test ELISA, Électrophorèse sur gel de polyacrylamide, Épitopes.
English descriptors
- KwdEn :
- Amino Acids (chemistry), Animals, Antibodies, Monoclonal (immunology), Antibodies, Protozoan (blood), Antibodies, Protozoan (immunology), Antigens, Protozoan (chemistry), Antigens, Protozoan (immunology), Blotting, Western, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Epitope Mapping, Epitopes (analysis), Epitopes (chemistry), Epitopes (immunology), Humans, Immune Sera (immunology), Mice, Models, Molecular, Plasmodium falciparum (enzymology), Plasmodium falciparum (immunology), Rabbits, Tetrahydrofolate Dehydrogenase (chemistry), Tetrahydrofolate Dehydrogenase (immunology).
- MESH :
- chemical , analysis : Epitopes.
- chemical , blood : Antibodies, Protozoan.
- chemical , chemistry : Amino Acids, Antigens, Protozoan, Epitopes, Tetrahydrofolate Dehydrogenase.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Protozoan, Antigens, Protozoan, Epitopes, Immune Sera, Tetrahydrofolate Dehydrogenase.
- enzymology : Plasmodium falciparum.
- immunology : Plasmodium falciparum.
- Animals, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Epitope Mapping, Humans, Mice, Models, Molecular, Rabbits.
Abstract
The aim of this study was to develop site-specific antibodies as a tool to capture
A homology model of Pf-DHFR domain was employed to define an epitope for the development of site-specific antibodies against Pf-DHFR. The homology model suggested an exposed loop encompassing amino acid residues 64–100. A synthetic peptide of 37-mers whose sequence corresponded to the sequence of amino acid residues 64–100 of Pf-DHFR was synthesized and used to immunize mice for antibodies. Additionally, polyclonal antibodies recognizing a recombinant DHFR enzyme were produced in rabbits.
Serum from mice immunized with the 37-mer showed strong reactivity against both the immunizing peptide, recombinant DHFR and a preparation of crude antigen from
Url:
DOI: 10.1186/1475-2875-3-16
PubMed: 15193156
PubMed Central: 449723
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000564
- to stream Pmc, to step Curation: 000564
- to stream Pmc, to step Checkpoint: 001429
- to stream PubMed, to step Corpus: 002386
- to stream PubMed, to step Curation: 002386
- to stream PubMed, to step Checkpoint: 002260
- to stream Ncbi, to step Merge: 000285
- to stream Ncbi, to step Curation: 000285
- to stream Ncbi, to step Checkpoint: 000285
Links to Exploration step
PMC:449723Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from <italic>Plasmodium falciparum</italic>
</title>
<author><name sortKey="Alifrangis, Michael" sort="Alifrangis, Michael" uniqKey="Alifrangis M" first="Michael" last="Alifrangis">Michael Alifrangis</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Christensen, Inge T" sort="Christensen, Inge T" uniqKey="Christensen I" first="Inge T" last="Christensen">Inge T. Christensen</name>
<affiliation wicri:level="1"><nlm:aff id="I3">Department of Medicinal Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Medicinal Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen</wicri:regionArea>
<wicri:noRegion>DK-2100 Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I6">Present address: Novo Nordisk A/S, Novo Nordisk Park, Maaloev, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Present address: Novo Nordisk A/S, Novo Nordisk Park, Maaloev</wicri:regionArea>
<wicri:noRegion>Maaloev</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="J Rgensen, Flemming S" sort="J Rgensen, Flemming S" uniqKey="J Rgensen F" first="Flemming S" last="J Rgensen">Flemming S. J Rgensen</name>
<affiliation wicri:level="1"><nlm:aff id="I3">Department of Medicinal Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Medicinal Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen</wicri:regionArea>
<wicri:noRegion>DK-2100 Copenhagen</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="R Nn, Anita M" sort="R Nn, Anita M" uniqKey="R Nn A" first="Anita M" last="R Nn">Anita M. R Nn</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Weng, Jimmy E" sort="Weng, Jimmy E" uniqKey="Weng J" first="Jimmy E" last="Weng">Jimmy E. Weng</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Ming" sort="Chen, Ming" uniqKey="Chen M" first="Ming" last="Chen">Ming Chen</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bygbjerg, Ib C" sort="Bygbjerg, Ib C" uniqKey="Bygbjerg I" first="Ib C" last="Bygbjerg">Ib C. Bygbjerg</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sirawaraporn, Worachart" sort="Sirawaraporn, Worachart" uniqKey="Sirawaraporn W" first="Worachart" last="Sirawaraporn">Worachart Sirawaraporn</name>
<affiliation wicri:level="1"><nlm:aff id="I5">Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand</nlm:aff>
<country xml:lang="fr">Thaïlande</country>
<wicri:regionArea>Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok</wicri:regionArea>
<wicri:noRegion>Bangkok</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Palarasah, Yaseelan" sort="Palarasah, Yaseelan" uniqKey="Palarasah Y" first="Yaseelan" last="Palarasah">Yaseelan Palarasah</name>
<affiliation wicri:level="3"><nlm:aff id="I4">Immunological Research Laboratory, State Serum Institute, Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Immunological Research Laboratory, State Serum Institute, Copenhagen</wicri:regionArea>
<placeName><settlement type="city">Copenhague</settlement>
<region type="région" nuts="2">Hovedstaden</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Koch, Claus" sort="Koch, Claus" uniqKey="Koch C" first="Claus" last="Koch">Claus Koch</name>
<affiliation wicri:level="3"><nlm:aff id="I4">Immunological Research Laboratory, State Serum Institute, Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Immunological Research Laboratory, State Serum Institute, Copenhagen</wicri:regionArea>
<placeName><settlement type="city">Copenhague</settlement>
<region type="région" nuts="2">Hovedstaden</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">15193156</idno>
<idno type="pmc">449723</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC449723</idno>
<idno type="RBID">PMC:449723</idno>
<idno type="doi">10.1186/1475-2875-3-16</idno>
<date when="2004">2004</date>
<idno type="wicri:Area/Pmc/Corpus">000564</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000564</idno>
<idno type="wicri:Area/Pmc/Curation">000564</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000564</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001429</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001429</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:15193156</idno>
<idno type="wicri:Area/PubMed/Corpus">002386</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002386</idno>
<idno type="wicri:Area/PubMed/Curation">002386</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002386</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002260</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002260</idno>
<idno type="wicri:Area/Ncbi/Merge">000285</idno>
<idno type="wicri:Area/Ncbi/Curation">000285</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000285</idno>
<idno type="wicri:Area/Main/Merge">003091</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from <italic>Plasmodium falciparum</italic>
</title>
<author><name sortKey="Alifrangis, Michael" sort="Alifrangis, Michael" uniqKey="Alifrangis M" first="Michael" last="Alifrangis">Michael Alifrangis</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Christensen, Inge T" sort="Christensen, Inge T" uniqKey="Christensen I" first="Inge T" last="Christensen">Inge T. Christensen</name>
<affiliation wicri:level="1"><nlm:aff id="I3">Department of Medicinal Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Medicinal Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen</wicri:regionArea>
<wicri:noRegion>DK-2100 Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I6">Present address: Novo Nordisk A/S, Novo Nordisk Park, Maaloev, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Present address: Novo Nordisk A/S, Novo Nordisk Park, Maaloev</wicri:regionArea>
<wicri:noRegion>Maaloev</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="J Rgensen, Flemming S" sort="J Rgensen, Flemming S" uniqKey="J Rgensen F" first="Flemming S" last="J Rgensen">Flemming S. J Rgensen</name>
<affiliation wicri:level="1"><nlm:aff id="I3">Department of Medicinal Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Medicinal Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen</wicri:regionArea>
<wicri:noRegion>DK-2100 Copenhagen</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="R Nn, Anita M" sort="R Nn, Anita M" uniqKey="R Nn A" first="Anita M" last="R Nn">Anita M. R Nn</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Weng, Jimmy E" sort="Weng, Jimmy E" uniqKey="Weng J" first="Jimmy E" last="Weng">Jimmy E. Weng</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Ming" sort="Chen, Ming" uniqKey="Chen M" first="Ming" last="Chen">Ming Chen</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bygbjerg, Ib C" sort="Bygbjerg, Ib C" uniqKey="Bygbjerg I" first="Ib C" last="Bygbjerg">Ib C. Bygbjerg</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen</wicri:regionArea>
<wicri:noRegion>University of Copenhagen</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="I2">Department of Infectious Diseases, Copenhagen University Hospital, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Infectious Diseases, Copenhagen University Hospital</wicri:regionArea>
<wicri:noRegion>Copenhagen University Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sirawaraporn, Worachart" sort="Sirawaraporn, Worachart" uniqKey="Sirawaraporn W" first="Worachart" last="Sirawaraporn">Worachart Sirawaraporn</name>
<affiliation wicri:level="1"><nlm:aff id="I5">Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand</nlm:aff>
<country xml:lang="fr">Thaïlande</country>
<wicri:regionArea>Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok</wicri:regionArea>
<wicri:noRegion>Bangkok</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Palarasah, Yaseelan" sort="Palarasah, Yaseelan" uniqKey="Palarasah Y" first="Yaseelan" last="Palarasah">Yaseelan Palarasah</name>
<affiliation wicri:level="3"><nlm:aff id="I4">Immunological Research Laboratory, State Serum Institute, Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Immunological Research Laboratory, State Serum Institute, Copenhagen</wicri:regionArea>
<placeName><settlement type="city">Copenhague</settlement>
<region type="région" nuts="2">Hovedstaden</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Koch, Claus" sort="Koch, Claus" uniqKey="Koch C" first="Claus" last="Koch">Claus Koch</name>
<affiliation wicri:level="3"><nlm:aff id="I4">Immunological Research Laboratory, State Serum Institute, Copenhagen, Denmark</nlm:aff>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Immunological Research Laboratory, State Serum Institute, Copenhagen</wicri:regionArea>
<placeName><settlement type="city">Copenhague</settlement>
<region type="région" nuts="2">Hovedstaden</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Malaria Journal</title>
<idno type="eISSN">1475-2875</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (chemistry)</term>
<term>Animals</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Antibodies, Protozoan (blood)</term>
<term>Antibodies, Protozoan (immunology)</term>
<term>Antigens, Protozoan (chemistry)</term>
<term>Antigens, Protozoan (immunology)</term>
<term>Blotting, Western</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Epitope Mapping</term>
<term>Epitopes (analysis)</term>
<term>Epitopes (chemistry)</term>
<term>Epitopes (immunology)</term>
<term>Humans</term>
<term>Immune Sera (immunology)</term>
<term>Mice</term>
<term>Models, Molecular</term>
<term>Plasmodium falciparum (enzymology)</term>
<term>Plasmodium falciparum (immunology)</term>
<term>Rabbits</term>
<term>Tetrahydrofolate Dehydrogenase (chemistry)</term>
<term>Tetrahydrofolate Dehydrogenase (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés ()</term>
<term>Animaux</term>
<term>Anticorps antiprotozoaires (immunologie)</term>
<term>Anticorps antiprotozoaires (sang)</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Antigènes de protozoaire ()</term>
<term>Antigènes de protozoaire (immunologie)</term>
<term>Cartographie épitopique</term>
<term>Dihydrofolate reductase ()</term>
<term>Dihydrofolate reductase (immunologie)</term>
<term>Humains</term>
<term>Lapins</term>
<term>Modèles moléculaires</term>
<term>Plasmodium falciparum (enzymologie)</term>
<term>Plasmodium falciparum (immunologie)</term>
<term>Souris</term>
<term>Sérums immuns (immunologie)</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
<term>Épitopes ()</term>
<term>Épitopes (analyse)</term>
<term>Épitopes (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Epitopes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Protozoan</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Amino Acids</term>
<term>Antigens, Protozoan</term>
<term>Epitopes</term>
<term>Tetrahydrofolate Dehydrogenase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Antibodies, Protozoan</term>
<term>Antigens, Protozoan</term>
<term>Epitopes</term>
<term>Immune Sera</term>
<term>Tetrahydrofolate Dehydrogenase</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Plasmodium falciparum</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plasmodium falciparum</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiprotozoaires</term>
<term>Anticorps monoclonaux</term>
<term>Antigènes de protozoaire</term>
<term>Dihydrofolate reductase</term>
<term>Plasmodium falciparum</term>
<term>Sérums immuns</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plasmodium falciparum</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiprotozoaires</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Blotting, Western</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Epitope Mapping</term>
<term>Humans</term>
<term>Mice</term>
<term>Models, Molecular</term>
<term>Rabbits</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Acides aminés</term>
<term>Animaux</term>
<term>Antigènes de protozoaire</term>
<term>Cartographie épitopique</term>
<term>Dihydrofolate reductase</term>
<term>Humains</term>
<term>Lapins</term>
<term>Modèles moléculaires</term>
<term>Souris</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
<term>Épitopes</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The aim of this study was to develop site-specific antibodies as a tool to capture <italic>Plasmodium falciparum</italic>
-dihydrofolate reductase (Pf-DHFR) from blood samples from <italic>P. falciparum </italic>
infected individuals in order to detect, in a sandwich ELISA, structural alterations due to point mutations in the gene coding for Pf-DHFR. Furthermore, we wanted to study the potential use of homology models in general and of Pf-DHFR in particular in predicting antigenic malarial surface epitopes.</p>
</sec>
<sec sec-type="methods"><title>Methods</title>
<p>A homology model of Pf-DHFR domain was employed to define an epitope for the development of site-specific antibodies against Pf-DHFR. The homology model suggested an exposed loop encompassing amino acid residues 64–100. A synthetic peptide of 37-mers whose sequence corresponded to the sequence of amino acid residues 64–100 of Pf-DHFR was synthesized and used to immunize mice for antibodies. Additionally, polyclonal antibodies recognizing a recombinant DHFR enzyme were produced in rabbits.</p>
</sec>
<sec><title>Results and conclusions</title>
<p>Serum from mice immunized with the 37-mer showed strong reactivity against both the immunizing peptide, recombinant DHFR and a preparation of crude antigen from <italic>P. falciparum </italic>
infected red blood cells. Five monoclonal antibodies were obtained, one of which showed reactivity towards crude antigen prepared from <italic>P. falciparum </italic>
infected red cells. Western blot analysis revealed that both the polyclonal and monoclonal antibodies recognized Pf-DHFR. Our study provides insight into the potential use of homology models in general and of Pf-DHFR in particular in predicting antigenic malarial surface epitopes.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003091 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003091 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Merge |type= RBID |clé= PMC:449723 |texte= Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:15193156" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |