Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum.
Identifieur interne : 002386 ( PubMed/Corpus ); précédent : 002385; suivant : 002387Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum.
Auteurs : Michael Alifrangis ; Inge T. Christensen ; Flemming S. J Rgensen ; Anita M. R Nn ; Jimmy E. Weng ; Ming Chen ; Ib C. Bygbjerg ; Worachart Sirawaraporn ; Yaseelan Palarasah ; Claus KochSource :
- Malaria journal [ 1475-2875 ] ; 2004.
English descriptors
- KwdEn :
- Amino Acids (chemistry), Animals, Antibodies, Monoclonal (immunology), Antibodies, Protozoan (blood), Antibodies, Protozoan (immunology), Antigens, Protozoan (chemistry), Antigens, Protozoan (immunology), Blotting, Western, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Epitope Mapping, Epitopes (analysis), Epitopes (chemistry), Epitopes (immunology), Humans, Immune Sera (immunology), Mice, Models, Molecular, Plasmodium falciparum (enzymology), Plasmodium falciparum (immunology), Rabbits, Tetrahydrofolate Dehydrogenase (chemistry), Tetrahydrofolate Dehydrogenase (immunology).
- MESH :
- chemical , analysis : Epitopes.
- chemical , blood : Antibodies, Protozoan.
- chemical , chemistry : Amino Acids, Antigens, Protozoan, Epitopes, Tetrahydrofolate Dehydrogenase.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Protozoan, Antigens, Protozoan, Epitopes, Immune Sera, Tetrahydrofolate Dehydrogenase.
- enzymology : Plasmodium falciparum.
- immunology : Plasmodium falciparum.
- Animals, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Epitope Mapping, Humans, Mice, Models, Molecular, Rabbits.
Abstract
The aim of this study was to develop site-specific antibodies as a tool to capture Plasmodium falciparum-dihydrofolate reductase (Pf-DHFR) from blood samples from P. falciparum infected individuals in order to detect, in a sandwich ELISA, structural alterations due to point mutations in the gene coding for Pf-DHFR. Furthermore, we wanted to study the potential use of homology models in general and of Pf-DHFR in particular in predicting antigenic malarial surface epitopes.
DOI: 10.1186/1475-2875-3-16
PubMed: 15193156
Links to Exploration step
pubmed:15193156Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum.</title><author><name sortKey="Alifrangis, Michael" sort="Alifrangis, Michael" uniqKey="Alifrangis M" first="Michael" last="Alifrangis">Michael Alifrangis</name><affiliation><nlm:affiliation>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark. malif@biobase.dk</nlm:affiliation></affiliation></author><author><name sortKey="Christensen, Inge T" sort="Christensen, Inge T" uniqKey="Christensen I" first="Inge T" last="Christensen">Inge T. Christensen</name></author><author><name sortKey="J Rgensen, Flemming S" sort="J Rgensen, Flemming S" uniqKey="J Rgensen F" first="Flemming S" last="J Rgensen">Flemming S. J Rgensen</name></author><author><name sortKey="R Nn, Anita M" sort="R Nn, Anita M" uniqKey="R Nn A" first="Anita M" last="R Nn">Anita M. R Nn</name></author><author><name sortKey="Weng, Jimmy E" sort="Weng, Jimmy E" uniqKey="Weng J" first="Jimmy E" last="Weng">Jimmy E. Weng</name></author><author><name sortKey="Chen, Ming" sort="Chen, Ming" uniqKey="Chen M" first="Ming" last="Chen">Ming Chen</name></author><author><name sortKey="Bygbjerg, Ib C" sort="Bygbjerg, Ib C" uniqKey="Bygbjerg I" first="Ib C" last="Bygbjerg">Ib C. Bygbjerg</name></author><author><name sortKey="Sirawaraporn, Worachart" sort="Sirawaraporn, Worachart" uniqKey="Sirawaraporn W" first="Worachart" last="Sirawaraporn">Worachart Sirawaraporn</name></author><author><name sortKey="Palarasah, Yaseelan" sort="Palarasah, Yaseelan" uniqKey="Palarasah Y" first="Yaseelan" last="Palarasah">Yaseelan Palarasah</name></author><author><name sortKey="Koch, Claus" sort="Koch, Claus" uniqKey="Koch C" first="Claus" last="Koch">Claus Koch</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15193156</idno><idno type="pmid">15193156</idno><idno type="doi">10.1186/1475-2875-3-16</idno><idno type="wicri:Area/PubMed/Corpus">002386</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002386</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum.</title><author><name sortKey="Alifrangis, Michael" sort="Alifrangis, Michael" uniqKey="Alifrangis M" first="Michael" last="Alifrangis">Michael Alifrangis</name><affiliation><nlm:affiliation>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark. malif@biobase.dk</nlm:affiliation></affiliation></author><author><name sortKey="Christensen, Inge T" sort="Christensen, Inge T" uniqKey="Christensen I" first="Inge T" last="Christensen">Inge T. Christensen</name></author><author><name sortKey="J Rgensen, Flemming S" sort="J Rgensen, Flemming S" uniqKey="J Rgensen F" first="Flemming S" last="J Rgensen">Flemming S. J Rgensen</name></author><author><name sortKey="R Nn, Anita M" sort="R Nn, Anita M" uniqKey="R Nn A" first="Anita M" last="R Nn">Anita M. R Nn</name></author><author><name sortKey="Weng, Jimmy E" sort="Weng, Jimmy E" uniqKey="Weng J" first="Jimmy E" last="Weng">Jimmy E. Weng</name></author><author><name sortKey="Chen, Ming" sort="Chen, Ming" uniqKey="Chen M" first="Ming" last="Chen">Ming Chen</name></author><author><name sortKey="Bygbjerg, Ib C" sort="Bygbjerg, Ib C" uniqKey="Bygbjerg I" first="Ib C" last="Bygbjerg">Ib C. Bygbjerg</name></author><author><name sortKey="Sirawaraporn, Worachart" sort="Sirawaraporn, Worachart" uniqKey="Sirawaraporn W" first="Worachart" last="Sirawaraporn">Worachart Sirawaraporn</name></author><author><name sortKey="Palarasah, Yaseelan" sort="Palarasah, Yaseelan" uniqKey="Palarasah Y" first="Yaseelan" last="Palarasah">Yaseelan Palarasah</name></author><author><name sortKey="Koch, Claus" sort="Koch, Claus" uniqKey="Koch C" first="Claus" last="Koch">Claus Koch</name></author></analytic><series><title level="j">Malaria journal</title><idno type="eISSN">1475-2875</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (chemistry)</term><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Protozoan (blood)</term><term>Antibodies, Protozoan (immunology)</term><term>Antigens, Protozoan (chemistry)</term><term>Antigens, Protozoan (immunology)</term><term>Blotting, Western</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitope Mapping</term><term>Epitopes (analysis)</term><term>Epitopes (chemistry)</term><term>Epitopes (immunology)</term><term>Humans</term><term>Immune Sera (immunology)</term><term>Mice</term><term>Models, Molecular</term><term>Plasmodium falciparum (enzymology)</term><term>Plasmodium falciparum (immunology)</term><term>Rabbits</term><term>Tetrahydrofolate Dehydrogenase (chemistry)</term><term>Tetrahydrofolate Dehydrogenase (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Epitopes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Protozoan</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Amino Acids</term><term>Antigens, Protozoan</term><term>Epitopes</term><term>Tetrahydrofolate Dehydrogenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Protozoan</term><term>Antigens, Protozoan</term><term>Epitopes</term><term>Immune Sera</term><term>Tetrahydrofolate Dehydrogenase</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plasmodium falciparum</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plasmodium falciparum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitope Mapping</term><term>Humans</term><term>Mice</term><term>Models, Molecular</term><term>Rabbits</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to develop site-specific antibodies as a tool to capture Plasmodium falciparum-dihydrofolate reductase (Pf-DHFR) from blood samples from P. falciparum infected individuals in order to detect, in a sandwich ELISA, structural alterations due to point mutations in the gene coding for Pf-DHFR. Furthermore, we wanted to study the potential use of homology models in general and of Pf-DHFR in particular in predicting antigenic malarial surface epitopes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15193156</PMID><DateCompleted><Year>2004</Year><Month>08</Month><Day>30</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1475-2875</ISSN><JournalIssue CitedMedium="Internet"><Volume>3</Volume><PubDate><Year>2004</Year><Month>Jun</Month><Day>12</Day></PubDate></JournalIssue><Title>Malaria journal</Title><ISOAbbreviation>Malar. J.</ISOAbbreviation></Journal><ArticleTitle>Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum.</ArticleTitle><Pagination><MedlinePgn>16</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The aim of this study was to develop site-specific antibodies as a tool to capture Plasmodium falciparum-dihydrofolate reductase (Pf-DHFR) from blood samples from P. falciparum infected individuals in order to detect, in a sandwich ELISA, structural alterations due to point mutations in the gene coding for Pf-DHFR. Furthermore, we wanted to study the potential use of homology models in general and of Pf-DHFR in particular in predicting antigenic malarial surface epitopes.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">A homology model of Pf-DHFR domain was employed to define an epitope for the development of site-specific antibodies against Pf-DHFR. The homology model suggested an exposed loop encompassing amino acid residues 64-100. A synthetic peptide of 37-mers whose sequence corresponded to the sequence of amino acid residues 64-100 of Pf-DHFR was synthesized and used to immunize mice for antibodies. Additionally, polyclonal antibodies recognizing a recombinant DHFR enzyme were produced in rabbits.</AbstractText><AbstractText Label="RESULTS AND CONCLUSIONS" NlmCategory="CONCLUSIONS">Serum from mice immunized with the 37-mer showed strong reactivity against both the immunizing peptide, recombinant DHFR and a preparation of crude antigen from P. falciparum infected red blood cells. Five monoclonal antibodies were obtained, one of which showed reactivity towards crude antigen prepared from P. falciparum infected red cells. Western blot analysis revealed that both the polyclonal and monoclonal antibodies recognized Pf-DHFR. Our study provides insight into the potential use of homology models in general and of Pf-DHFR in particular in predicting antigenic malarial surface epitopes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Alifrangis</LastName><ForeName>Michael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark. malif@biobase.dk</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Christensen</LastName><ForeName>Inge T</ForeName><Initials>IT</Initials></Author><Author ValidYN="Y"><LastName>Jørgensen</LastName><ForeName>Flemming S</ForeName><Initials>FS</Initials></Author><Author ValidYN="Y"><LastName>Rønn</LastName><ForeName>Anita M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Weng</LastName><ForeName>Jimmy E</ForeName><Initials>JE</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Ming</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Bygbjerg</LastName><ForeName>Ib C</ForeName><Initials>IC</Initials></Author><Author ValidYN="Y"><LastName>Sirawaraporn</LastName><ForeName>Worachart</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Palarasah</LastName><ForeName>Yaseelan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Koch</LastName><ForeName>Claus</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2004</Year><Month>06</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Malar J</MedlineTA><NlmUniqueID>101139802</NlmUniqueID><ISSNLinking>1475-2875</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000596">Amino Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000913">Antibodies, Protozoan</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000953">Antigens, Protozoan</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000939">Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007106">Immune Sera</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.5.1.3</RegistryNumber><NameOfSubstance UI="D013762">Tetrahydrofolate Dehydrogenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000596" MajorTopicYN="N">Amino Acids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000913" MajorTopicYN="N">Antibodies, Protozoan</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000953" MajorTopicYN="N">Antigens, Protozoan</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018604" MajorTopicYN="N">Epitope Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007106" MajorTopicYN="N">Immune Sera</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010963" MajorTopicYN="N">Plasmodium falciparum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011817" MajorTopicYN="N">Rabbits</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013762" MajorTopicYN="N">Tetrahydrofolate Dehydrogenase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>01</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>06</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>6</Month><Day>15</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>8</Month><Day>31</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>6</Month><Day>15</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15193156</ArticleId><ArticleId IdType="doi">10.1186/1475-2875-3-16</ArticleId><ArticleId IdType="pii">1475-2875-3-16</ArticleId><ArticleId IdType="pmc">PMC449723</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nat Struct Biol. 2003 May;10(5):357-65</Citation><ArticleIdList><ArticleId IdType="pubmed">12704428</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Pathol. 2000 Jun;53(3):111-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10897328</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2000 Jan 1;28(1):235-42</Citation><ArticleIdList><ArticleId IdType="pubmed">10592235</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Trop Med Hyg. 1988 Oct;39(4):328-36</Citation><ArticleIdList><ArticleId IdType="pubmed">3142285</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioorg Med Chem. 1999 Jun;7(6):1003-11</Citation><ArticleIdList><ArticleId IdType="pubmed">10428368</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1124-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9037017</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Biol. 1994 Mar;1(3):186-94</Citation><ArticleIdList><ArticleId IdType="pubmed">7656037</ArticleId></ArticleIdList></Reference><Reference><Citation>Pept Res. 1994 Jul-Aug;7(4):224-8</Citation><ArticleIdList><ArticleId IdType="pubmed">7535133</ArticleId></ArticleIdList></Reference><Reference><Citation>FASEB J. 1995 Jan;9(1):37-42</Citation><ArticleIdList><ArticleId IdType="pubmed">7821757</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1990 Apr;87(8):3018-22</Citation><ArticleIdList><ArticleId IdType="pubmed">2183222</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1990 Apr;87(8):3014-7</Citation><ArticleIdList><ArticleId IdType="pubmed">2183221</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1988 Dec;85(23):9114-8</Citation><ArticleIdList><ArticleId IdType="pubmed">2904149</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002386 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002386 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:15193156
|texte= Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:15193156" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |