MersV1, Main, Merge, bibRecord, 001C81

Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes

Identifieur interne : 001C81 ( Main/Merge ); précédent : 001C80; suivant : 001C82

Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes

Auteurs :

Source :

Coronaviruses ; 2014.

RBID : PMC:4726977

Descripteurs français

KwdFr :
- ADN complémentaire (génétique), Animaux, Chromosomes artificiels de bactérie (génétique), Coronavirus (génétique), Cricetinae, Escherichia coli, Génie génétique, Génétique inverse, Humains, Lignée cellulaire, Plasmides (génétique), Plasmides (isolement et purification), Transformation génétique.
MESH :
- génétique : ADN complémentaire, Chromosomes artificiels de bactérie, Coronavirus, Plasmides.
- isolement et purification : Plasmides.
- Animaux, Cricetinae, Escherichia coli, Génie génétique, Génétique inverse, Humains, Lignée cellulaire, Transformation génétique.

English descriptors

KwdEn :
- Animals, Cell Line, Chromosomes, Artificial, Bacterial (genetics), Coronavirus (genetics), Cricetinae, DNA, Complementary (genetics), Escherichia coli, Genetic Engineering, Humans, Plasmids (genetics), Plasmids (isolation & purification), Reverse Genetics, Transformation, Genetic.
MESH :
- chemical , genetics : DNA, Complementary.
- genetics : Chromosomes, Artificial, Bacterial, Coronavirus, Plasmids.
- isolation & purification : Plasmids.
- Animals, Cell Line, Cricetinae, Escherichia coli, Genetic Engineering, Humans, Reverse Genetics, Transformation, Genetic.

Abstract

The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last 13 years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726977

DOI: 10.1007/978-1-4939-2438-7_13
PubMed: 25720478
PubMed Central: 4726977

Links to Exploration step

PMC:4726977

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p id="Par1">The large size of the coronavirus (CoV) genome (around 30 kb) and the instability in bacteria of plasmids carrying CoV replicase sequences represent serious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last 13 years. Here we describe the engineering of CoV full-length cDNA clones as bacterial artificial chromosomes (BACs), using the Middle East respiratory syndrome CoV (MERS-CoV) as a model.</p>
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Serveur d'exploration MERS

Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes

Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.