Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.
Identifieur interne : 000A10 ( Main/Merge ); précédent : 000A09; suivant : 000A11Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.
Auteurs : Kunse Lee [Corée du Sud] ; Hae Li Ko [Corée du Sud] ; Eun-Young Lee [Corée du Sud] ; Hyo-Jung Park [Corée du Sud] ; Young Seok Kim [Corée du Sud] ; Yeon-Sook Kim [Corée du Sud] ; Nam-Hyuk Cho [Corée du Sud] ; Man-Seong Park [Corée du Sud] ; Sang-Myeong Lee [Corée du Sud] ; Jihye Kim [Corée du Sud] ; Hun Kim [Corée du Sud] ; Baik Lin Seong [Corée du Sud] ; Jae-Hwan Nam [Corée du Sud]Source :
- Microbiology and immunology [ 1348-0421 ] ; 2018.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (isolement et purification), Anticorps antiviraux (sang), Anticorps monoclonaux, Antigènes viraux (immunologie), Antigènes viraux (isolement et purification), Baculoviridae (génétique), Cellules Sf9, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Escherichia coli (génétique), Expression des gènes, Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (immunologie), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (immunologie), Infections à coronavirus (virologie), Liaison aux protéines (génétique), Liaison aux protéines (immunologie), Motifs et domaines d'intéraction protéique, Protéines de la matrice virale (génétique), Protéines de la matrice virale (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Rat Wistar, Rats, Reproductibilité des résultats, Récepteurs viraux (immunologie), Sensibilité et spécificité, Test ELISA (), Tests immunologiques ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Baculoviridae, Coronavirus du syndrome respiratoire du Moyen-Orient, Escherichia coli, Glycoprotéine de spicule des coronavirus, Liaison aux protéines, Protéines de la matrice virale, Protéines recombinantes.
- immunologie : Anticorps antiviraux, Antigènes viraux, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Infections à coronavirus, Liaison aux protéines, Protéines de la matrice virale, Protéines recombinantes, Récepteurs viraux.
- isolement et purification : Anticorps antiviraux, Antigènes viraux.
- sang : Anticorps antiviraux.
- virologie : Infections à coronavirus.
- Animaux, Anticorps monoclonaux, Cellules Sf9, Expression des gènes, Humains, Motifs et domaines d'intéraction protéique, Rat Wistar, Rats, Reproductibilité des résultats, Sensibilité et spécificité, Test ELISA, Tests immunologiques.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal, Antibodies, Viral (blood), Antibodies, Viral (immunology), Antibodies, Viral (isolation & purification), Antigens, Viral (immunology), Antigens, Viral (isolation & purification), Baculoviridae (genetics), Coronavirus Infections (diagnosis), Coronavirus Infections (immunology), Coronavirus Infections (virology), Enzyme-Linked Immunosorbent Assay (methods), Escherichia coli (genetics), Gene Expression, Humans, Immunologic Tests (methods), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Protein Binding (genetics), Protein Binding (immunology), Protein Interaction Domains and Motifs, Rats, Rats, Wistar, Receptors, Virus (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Reproducibility of Results, Sensitivity and Specificity, Sf9 Cells, Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (immunology), Viral Matrix Proteins (genetics), Viral Matrix Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Matrix Proteins.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Receptors, Virus, Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Matrix Proteins.
- chemical , isolation & purification : Antibodies, Viral, Antigens, Viral.
- chemical : Antibodies, Monoclonal.
- diagnosis : Coronavirus Infections.
- genetics : Baculoviridae, Escherichia coli, Middle East Respiratory Syndrome Coronavirus, Protein Binding.
- immunology : Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus, Protein Binding.
- methods : Enzyme-Linked Immunosorbent Assay, Immunologic Tests.
- virology : Coronavirus Infections.
- Animals, Gene Expression, Humans, Protein Interaction Domains and Motifs, Rats, Rats, Wistar, Reproducibility of Results, Sensitivity and Specificity, Sf9 Cells.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.
DOI: 10.1111/1348-0421.12643
PubMed: 30117617
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000807
- to stream PubMed, to step Curation: 000807
- to stream PubMed, to step Checkpoint: 000952
- to stream Ncbi, to step Merge: 001F35
- to stream Ncbi, to step Curation: 001F35
- to stream Ncbi, to step Checkpoint: 001F35
Links to Exploration step
pubmed:30117617Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.</title>
<author><name sortKey="Lee, Kunse" sort="Lee, Kunse" uniqKey="Lee K" first="Kunse" last="Lee">Kunse Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ko, Hae Li" sort="Ko, Hae Li" uniqKey="Ko H" first="Hae Li" last="Ko">Hae Li Ko</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Eun Young" sort="Lee, Eun Young" uniqKey="Lee E" first="Eun-Young" last="Lee">Eun-Young Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Park, Hyo Jung" sort="Park, Hyo Jung" uniqKey="Park H" first="Hyo-Jung" last="Park">Hyo-Jung Park</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Young Seok" sort="Kim, Young Seok" uniqKey="Kim Y" first="Young Seok" last="Kim">Young Seok Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Yeon Sook" sort="Kim, Yeon Sook" uniqKey="Kim Y" first="Yeon-Sook" last="Kim">Yeon-Sook Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon 35015, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon 35015</wicri:regionArea>
<wicri:noRegion>Daejeon 35015</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cho, Nam Hyuk" sort="Cho, Nam Hyuk" uniqKey="Cho N" first="Nam-Hyuk" last="Cho">Nam-Hyuk Cho</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Man Seong" sort="Park, Man Seong" uniqKey="Park M" first="Man-Seong" last="Park">Man-Seong Park</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 02841, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 02841</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lee, Sang Myeong" sort="Lee, Sang Myeong" uniqKey="Lee S" first="Sang-Myeong" last="Lee">Sang-Myeong Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of, Biotechnology, Chonbuk National University, Iksan 570-752, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of, Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea>
<wicri:noRegion>Iksan 570-752</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Jihye" sort="Kim, Jihye" uniqKey="Kim J" first="Jihye" last="Kim">Jihye Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701</wicri:regionArea>
<wicri:noRegion>Yongin 446-701</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Hun" sort="Kim, Hun" uniqKey="Kim H" first="Hun" last="Kim">Hun Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Bio Research and Development, SK Chemicals, Seongnam, Gyeonggi-do 13493, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Bio Research and Development, SK Chemicals, Seongnam, Gyeonggi-do 13493</wicri:regionArea>
<wicri:noRegion>Gyeonggi-do 13493</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Seong, Baik Lin" sort="Seong, Baik Lin" uniqKey="Seong B" first="Baik Lin" last="Seong">Baik Lin Seong</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2018">2018</date>
<idno type="RBID">pubmed:30117617</idno>
<idno type="pmid">30117617</idno>
<idno type="doi">10.1111/1348-0421.12643</idno>
<idno type="wicri:Area/PubMed/Corpus">000807</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000807</idno>
<idno type="wicri:Area/PubMed/Curation">000807</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000807</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000952</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000952</idno>
<idno type="wicri:Area/Ncbi/Merge">001F35</idno>
<idno type="wicri:Area/Ncbi/Curation">001F35</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001F35</idno>
<idno type="wicri:Area/Main/Merge">000A10</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.</title>
<author><name sortKey="Lee, Kunse" sort="Lee, Kunse" uniqKey="Lee K" first="Kunse" last="Lee">Kunse Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ko, Hae Li" sort="Ko, Hae Li" uniqKey="Ko H" first="Hae Li" last="Ko">Hae Li Ko</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Eun Young" sort="Lee, Eun Young" uniqKey="Lee E" first="Eun-Young" last="Lee">Eun-Young Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Park, Hyo Jung" sort="Park, Hyo Jung" uniqKey="Park H" first="Hyo-Jung" last="Park">Hyo-Jung Park</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Young Seok" sort="Kim, Young Seok" uniqKey="Kim Y" first="Young Seok" last="Kim">Young Seok Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Yeon Sook" sort="Kim, Yeon Sook" uniqKey="Kim Y" first="Yeon-Sook" last="Kim">Yeon-Sook Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon 35015, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon 35015</wicri:regionArea>
<wicri:noRegion>Daejeon 35015</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cho, Nam Hyuk" sort="Cho, Nam Hyuk" uniqKey="Cho N" first="Nam-Hyuk" last="Cho">Nam-Hyuk Cho</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Man Seong" sort="Park, Man Seong" uniqKey="Park M" first="Man-Seong" last="Park">Man-Seong Park</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 02841, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 02841</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lee, Sang Myeong" sort="Lee, Sang Myeong" uniqKey="Lee S" first="Sang-Myeong" last="Lee">Sang-Myeong Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of, Biotechnology, Chonbuk National University, Iksan 570-752, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of, Biotechnology, Chonbuk National University, Iksan 570-752</wicri:regionArea>
<wicri:noRegion>Iksan 570-752</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Jihye" sort="Kim, Jihye" uniqKey="Kim J" first="Jihye" last="Kim">Jihye Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701</wicri:regionArea>
<wicri:noRegion>Yongin 446-701</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Hun" sort="Kim, Hun" uniqKey="Kim H" first="Hun" last="Kim">Hun Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Bio Research and Development, SK Chemicals, Seongnam, Gyeonggi-do 13493, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Bio Research and Development, SK Chemicals, Seongnam, Gyeonggi-do 13493</wicri:regionArea>
<wicri:noRegion>Gyeonggi-do 13493</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Seong, Baik Lin" sort="Seong, Baik Lin" uniqKey="Seong B" first="Baik Lin" last="Seong">Baik Lin Seong</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, Catholic University of Korea, Bucheon 14662</wicri:regionArea>
<wicri:noRegion>Bucheon 14662</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Microbiology and immunology</title>
<idno type="eISSN">1348-0421</idno>
<imprint><date when="2018" type="published">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Monoclonal</term>
<term>Antibodies, Viral (blood)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Antibodies, Viral (isolation & purification)</term>
<term>Antigens, Viral (immunology)</term>
<term>Antigens, Viral (isolation & purification)</term>
<term>Baculoviridae (genetics)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (immunology)</term>
<term>Coronavirus Infections (virology)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Escherichia coli (genetics)</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Immunologic Tests (methods)</term>
<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
<term>Middle East Respiratory Syndrome Coronavirus (immunology)</term>
<term>Protein Binding (genetics)</term>
<term>Protein Binding (immunology)</term>
<term>Protein Interaction Domains and Motifs</term>
<term>Rats</term>
<term>Rats, Wistar</term>
<term>Receptors, Virus (immunology)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
<term>Sf9 Cells</term>
<term>Spike Glycoprotein, Coronavirus (genetics)</term>
<term>Spike Glycoprotein, Coronavirus (immunology)</term>
<term>Viral Matrix Proteins (genetics)</term>
<term>Viral Matrix Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps antiviraux (isolement et purification)</term>
<term>Anticorps antiviraux (sang)</term>
<term>Anticorps monoclonaux</term>
<term>Antigènes viraux (immunologie)</term>
<term>Antigènes viraux (isolement et purification)</term>
<term>Baculoviridae (génétique)</term>
<term>Cellules Sf9</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term>
<term>Escherichia coli (génétique)</term>
<term>Expression des gènes</term>
<term>Glycoprotéine de spicule des coronavirus (génétique)</term>
<term>Glycoprotéine de spicule des coronavirus (immunologie)</term>
<term>Humains</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (immunologie)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Liaison aux protéines (génétique)</term>
<term>Liaison aux protéines (immunologie)</term>
<term>Motifs et domaines d'intéraction protéique</term>
<term>Protéines de la matrice virale (génétique)</term>
<term>Protéines de la matrice virale (immunologie)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Rat Wistar</term>
<term>Rats</term>
<term>Reproductibilité des résultats</term>
<term>Récepteurs viraux (immunologie)</term>
<term>Sensibilité et spécificité</term>
<term>Test ELISA ()</term>
<term>Tests immunologiques ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Recombinant Proteins</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term>
<term>Antigens, Viral</term>
<term>Receptors, Virus</term>
<term>Recombinant Proteins</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Viral</term>
<term>Antigens, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Baculoviridae</term>
<term>Escherichia coli</term>
<term>Middle East Respiratory Syndrome Coronavirus</term>
<term>Protein Binding</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Baculoviridae</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Escherichia coli</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Liaison aux protéines</term>
<term>Protéines de la matrice virale</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Antigènes viraux</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Infections à coronavirus</term>
<term>Liaison aux protéines</term>
<term>Protéines de la matrice virale</term>
<term>Protéines recombinantes</term>
<term>Récepteurs viraux</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Middle East Respiratory Syndrome Coronavirus</term>
<term>Protein Binding</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Antigènes viraux</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
<term>Immunologic Tests</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Protein Interaction Domains and Motifs</term>
<term>Rats</term>
<term>Rats, Wistar</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
<term>Sf9 Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Anticorps monoclonaux</term>
<term>Cellules Sf9</term>
<term>Expression des gènes</term>
<term>Humains</term>
<term>Motifs et domaines d'intéraction protéique</term>
<term>Rat Wistar</term>
<term>Rats</term>
<term>Reproductibilité des résultats</term>
<term>Sensibilité et spécificité</term>
<term>Test ELISA</term>
<term>Tests immunologiques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A10 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000A10 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Merge |type= RBID |clé= pubmed:30117617 |texte= Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:30117617" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |