Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.
Identifieur interne : 000807 ( PubMed/Corpus ); précédent : 000806; suivant : 000808Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.
Auteurs : Kunse Lee ; Hae Li Ko ; Eun-Young Lee ; Hyo-Jung Park ; Young Seok Kim ; Yeon-Sook Kim ; Nam-Hyuk Cho ; Man-Seong Park ; Sang-Myeong Lee ; Jihye Kim ; Hun Kim ; Baik Lin Seong ; Jae-Hwan NamSource :
- Microbiology and immunology [ 1348-0421 ] ; 2018.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal, Antibodies, Viral (blood), Antibodies, Viral (immunology), Antibodies, Viral (isolation & purification), Antigens, Viral (immunology), Antigens, Viral (isolation & purification), Baculoviridae (genetics), Coronavirus Infections (diagnosis), Coronavirus Infections (immunology), Coronavirus Infections (virology), Enzyme-Linked Immunosorbent Assay (methods), Escherichia coli (genetics), Gene Expression, Humans, Immunologic Tests (methods), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Protein Binding (genetics), Protein Binding (immunology), Protein Interaction Domains and Motifs, Rats, Rats, Wistar, Receptors, Virus (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Reproducibility of Results, Sensitivity and Specificity, Sf9 Cells, Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (immunology), Viral Matrix Proteins (genetics), Viral Matrix Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Matrix Proteins.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Receptors, Virus, Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Matrix Proteins.
- chemical , isolation & purification : Antibodies, Viral, Antigens, Viral.
- chemical : Antibodies, Monoclonal.
- diagnosis : Coronavirus Infections.
- genetics : Baculoviridae, Escherichia coli, Middle East Respiratory Syndrome Coronavirus, Protein Binding.
- immunology : Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus, Protein Binding.
- methods : Enzyme-Linked Immunosorbent Assay, Immunologic Tests.
- virology : Coronavirus Infections.
- Animals, Gene Expression, Humans, Protein Interaction Domains and Motifs, Rats, Rats, Wistar, Reproducibility of Results, Sensitivity and Specificity, Sf9 Cells.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.
DOI: 10.1111/1348-0421.12643
PubMed: 30117617
Links to Exploration step
pubmed:30117617Le document en format XML
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Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Park, Hyo Jung" sort="Park, Hyo Jung" uniqKey="Park H" first="Hyo-Jung" last="Park">Hyo-Jung Park</name><affiliation><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Young Seok" sort="Kim, Young Seok" uniqKey="Kim Y" first="Young Seok" last="Kim">Young Seok Kim</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Yeon Sook" sort="Kim, Yeon Sook" uniqKey="Kim Y" first="Yeon-Sook" last="Kim">Yeon-Sook Kim</name><affiliation><nlm:affiliation>Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon 35015, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Cho, Nam Hyuk" sort="Cho, Nam Hyuk" uniqKey="Cho N" first="Nam-Hyuk" last="Cho">Nam-Hyuk Cho</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Park, Man Seong" sort="Park, Man Seong" uniqKey="Park M" first="Man-Seong" last="Park">Man-Seong Park</name><affiliation><nlm:affiliation>Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 02841, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Sang Myeong" sort="Lee, Sang Myeong" uniqKey="Lee S" first="Sang-Myeong" last="Lee">Sang-Myeong Lee</name><affiliation><nlm:affiliation>Department of, Biotechnology, Chonbuk National University, Iksan 570-752, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Jihye" sort="Kim, Jihye" uniqKey="Kim J" first="Jihye" last="Kim">Jihye 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last="Seong">Baik Lin Seong</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name><affiliation><nlm:affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Microbiology and immunology</title><idno type="eISSN">1348-0421</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal</term><term>Antibodies, Viral (blood)</term><term>Antibodies, Viral (immunology)</term><term>Antibodies, Viral (isolation & purification)</term><term>Antigens, Viral 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Glycoprotein, Coronavirus (genetics)</term><term>Spike Glycoprotein, Coronavirus (immunology)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Matrix Proteins (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Recombinant Proteins</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Antigens, Viral</term><term>Receptors, Virus</term><term>Recombinant Proteins</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Viral</term><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="chemical" 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Motifs</term><term>Rats</term><term>Rats, Wistar</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Sf9 Cells</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30117617</PMID><DateCompleted><Year>2018</Year><Month>11</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1348-0421</ISSN><JournalIssue CitedMedium="Internet"><Volume>62</Volume><Issue>9</Issue><PubDate><Year>2018</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Microbiology and immunology</Title><ISOAbbreviation>Microbiol. Immunol.</ISOAbbreviation></Journal><ArticleTitle>Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.</ArticleTitle><Pagination><MedlinePgn>574-584</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/1348-0421.12643</ELocationID><Abstract><AbstractText>Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.</AbstractText><CopyrightInformation>© 2018 The Societies and John Wiley & Sons Australia, Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Kunse</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Bio Research and Development, SK Chemicals, Seongnam, Gyeonggi-do 13493, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ko</LastName><ForeName>Hae Li</ForeName><Initials>HL</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Eun-Young</ForeName><Initials>EY</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Hyo-Jung</ForeName><Initials>HJ</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Young Seok</ForeName><Initials>YS</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Yeon-Sook</ForeName><Initials>YS</Initials><AffiliationInfo><Affiliation>Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon 35015, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Nam-Hyuk</ForeName><Initials>NH</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Man-Seong</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul 02841, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sang-Myeong</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Department of, Biotechnology, Chonbuk National University, Iksan 570-752, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Jihye</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hun</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Bio Research and Development, SK Chemicals, Seongnam, Gyeonggi-do 13493, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Seong</LastName><ForeName>Baik Lin</ForeName><Initials>BL</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nam</LastName><ForeName>Jae-Hwan</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>16172MFDS268</GrantID><Agency>Ministry of Food and Drug Safety in 2016-2017</Agency><Country></Country></Grant><Grant><GrantID>HI15C2955</GrantID><Agency>Ministry of Health and Welfare, Korea</Agency><Country></Country></Grant><Grant><GrantID>NRF-2015M3A9B5030157</GrantID><Agency>Basic Science Research Program</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Australia</Country><MedlineTA>Microbiol Immunol</MedlineTA><NlmUniqueID>7703966</NlmUniqueID><ISSNLinking>0385-5600</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, 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MajorTopicYN="N">receptor binding domain</Keyword><Keyword MajorTopicYN="N">spike protein</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>05</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>07</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>08</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>8</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>11</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>8</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30117617</ArticleId><ArticleId IdType="doi">10.1111/1348-0421.12643</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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