Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.
Identifieur interne : 000807 ( PubMed/Corpus ); précédent : 000806; suivant : 000808Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.
Auteurs : Kunse Lee ; Hae Li Ko ; Eun-Young Lee ; Hyo-Jung Park ; Young Seok Kim ; Yeon-Sook Kim ; Nam-Hyuk Cho ; Man-Seong Park ; Sang-Myeong Lee ; Jihye Kim ; Hun Kim ; Baik Lin Seong ; Jae-Hwan NamSource :
- Microbiology and immunology [ 1348-0421 ] ; 2018.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal, Antibodies, Viral (blood), Antibodies, Viral (immunology), Antibodies, Viral (isolation & purification), Antigens, Viral (immunology), Antigens, Viral (isolation & purification), Baculoviridae (genetics), Coronavirus Infections (diagnosis), Coronavirus Infections (immunology), Coronavirus Infections (virology), Enzyme-Linked Immunosorbent Assay (methods), Escherichia coli (genetics), Gene Expression, Humans, Immunologic Tests (methods), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Protein Binding (genetics), Protein Binding (immunology), Protein Interaction Domains and Motifs, Rats, Rats, Wistar, Receptors, Virus (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Reproducibility of Results, Sensitivity and Specificity, Sf9 Cells, Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (immunology), Viral Matrix Proteins (genetics), Viral Matrix Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Matrix Proteins.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Receptors, Virus, Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Matrix Proteins.
- chemical , isolation & purification : Antibodies, Viral, Antigens, Viral.
- chemical : Antibodies, Monoclonal.
- diagnosis : Coronavirus Infections.
- genetics : Baculoviridae, Escherichia coli, Middle East Respiratory Syndrome Coronavirus, Protein Binding.
- immunology : Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus, Protein Binding.
- methods : Enzyme-Linked Immunosorbent Assay, Immunologic Tests.
- virology : Coronavirus Infections.
- Animals, Gene Expression, Humans, Protein Interaction Domains and Motifs, Rats, Rats, Wistar, Reproducibility of Results, Sensitivity and Specificity, Sf9 Cells.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.
DOI: 10.1111/1348-0421.12643
PubMed: 30117617
Links to Exploration step
pubmed:30117617Le document en format XML
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<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.</div>
</front>
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<DateCompleted><Year>2018</Year>
<Month>11</Month>
<Day>19</Day>
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<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>19</Day>
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<JournalIssue CitedMedium="Internet"><Volume>62</Volume>
<Issue>9</Issue>
<PubDate><Year>2018</Year>
<Month>Sep</Month>
</PubDate>
</JournalIssue>
<Title>Microbiology and immunology</Title>
<ISOAbbreviation>Microbiol. Immunol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.</ArticleTitle>
<Pagination><MedlinePgn>574-584</MedlinePgn>
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<Abstract><AbstractText>Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.</AbstractText>
<CopyrightInformation>© 2018 The Societies and John Wiley & Sons Australia, Ltd.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>Kunse</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Bio Research and Development, SK Chemicals, Seongnam, Gyeonggi-do 13493, Korea.</Affiliation>
</AffiliationInfo>
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<ForeName>Hae Li</ForeName>
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</AffiliationInfo>
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</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Seong</LastName>
<ForeName>Baik Lin</ForeName>
<Initials>BL</Initials>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Nam</LastName>
<ForeName>Jae-Hwan</ForeName>
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<AffiliationInfo><Affiliation>Department of Biotechnology, Catholic University of Korea, Bucheon 14662, Korea.</Affiliation>
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<Country></Country>
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<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
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<MeshHeading><DescriptorName UI="D007159" MajorTopicYN="N">Immunologic Tests</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
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<MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D054730" MajorTopicYN="N">Protein Interaction Domains and Motifs</DescriptorName>
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<MeshHeading><DescriptorName UI="D017208" MajorTopicYN="N">Rats, Wistar</DescriptorName>
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<MeshHeading><DescriptorName UI="D011991" MajorTopicYN="N">Receptors, Virus</DescriptorName>
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<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
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<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName>
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<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
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<MeshHeading><DescriptorName UI="D061987" MajorTopicYN="N">Sf9 Cells</DescriptorName>
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<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D014763" MajorTopicYN="N">Viral Matrix Proteins</DescriptorName>
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</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ELISA</Keyword>
<Keyword MajorTopicYN="N">Middle East respiratory syndrome coronavirus</Keyword>
<Keyword MajorTopicYN="N">receptor binding domain</Keyword>
<Keyword MajorTopicYN="N">spike protein</Keyword>
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<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>05</Month>
<Day>01</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2018</Year>
<Month>07</Month>
<Day>22</Day>
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<PubMedPubDate PubStatus="accepted"><Year>2018</Year>
<Month>08</Month>
<Day>08</Day>
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<PubMedPubDate PubStatus="pubmed"><Year>2018</Year>
<Month>8</Month>
<Day>18</Day>
<Hour>6</Hour>
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<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>11</Month>
<Day>20</Day>
<Hour>6</Hour>
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<PubMedPubDate PubStatus="entrez"><Year>2018</Year>
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<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">30117617</ArticleId>
<ArticleId IdType="doi">10.1111/1348-0421.12643</ArticleId>
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