Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus
Identifieur interne : 000654 ( Main/Exploration ); précédent : 000653; suivant : 000655Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus
Auteurs : Ruth Harvey ; Giada Mattiuzzo ; Mark Hassall ; Andrea Sieberg ; Marcel A. Müller ; Christian Drosten ; Peter Rigsby ; Christopher J. OxenfordSource :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2019.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) was detected in humans in 2012. Since then, sporadic outbreaks with primary transmission through dromedary camels to humans and outbreaks in healthcare settings have shown that MERS-CoV continues to pose a threat to human health. Several serologic assays for MERS-CoV have been developed globally. We describe a collaborative study to investigate the comparability of serologic assays for MERS-CoV and assess any benefit associated with the introduction of a standard reference reagent for MERS-CoV serology. Our study findings indicate that, when possible, laboratories should use a testing algorithm including
Url:
DOI: 10.3201/eid2510.190497
PubMed: 31423969
PubMed Central: 6759245
Affiliations:
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<front><div type="abstract" xml:lang="en"><p>Middle East respiratory syndrome coronavirus (MERS-CoV) was detected in humans in 2012. Since then, sporadic outbreaks with primary transmission through dromedary camels to humans and outbreaks in healthcare settings have shown that MERS-CoV continues to pose a threat to human health. Several serologic assays for MERS-CoV have been developed globally. We describe a collaborative study to investigate the comparability of serologic assays for MERS-CoV and assess any benefit associated with the introduction of a standard reference reagent for MERS-CoV serology. Our study findings indicate that, when possible, laboratories should use a testing algorithm including <underline>></underline>
2 tests to ensure correct diagnosis of MERS-CoV. We also demonstrate that the use of a reference reagent greatly improves the agreement between assays, enabling more consistent and therefore more meaningful comparisons between results.</p>
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