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Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus.

Identifieur interne : 000631 ( PubMed/Checkpoint ); précédent : 000630; suivant : 000632

Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus.

Auteurs : Ruth Harvey ; Giada Mattiuzzo ; Mark Hassall ; Andrea Sieberg ; Marcel A. Müller ; Christian Drosten ; Peter Rigsby ; Christopher J. Oxenford

Source :

Emerging infectious diseases [ 1080-6059 ] ; 2019.

RBID : pubmed:31423969

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) was detected in humans in 2012. Since then, sporadic outbreaks with primary transmission through dromedary camels to humans and outbreaks in healthcare settings have shown that MERS-CoV continues to pose a threat to human health. Several serologic assays for MERS-CoV have been developed globally. We describe a collaborative study to investigate the comparability of serologic assays for MERS-CoV and assess any benefit associated with the introduction of a standard reference reagent for MERS-CoV serology. Our study findings indicate that, when possible, laboratories should use a testing algorithm including >2 tests to ensure correct diagnosis of MERS-CoV. We also demonstrate that the use of a reference reagent greatly improves the agreement between assays, enabling more consistent and therefore more meaningful comparisons between results.

DOI: 10.3201/eid2510.190497
PubMed: 31423969

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<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) was detected in humans in 2012. Since then, sporadic outbreaks with primary transmission through dromedary camels to humans and outbreaks in healthcare settings have shown that MERS-CoV continues to pose a threat to human health. Several serologic assays for MERS-CoV have been developed globally. We describe a collaborative study to investigate the comparability of serologic assays for MERS-CoV and assess any benefit associated with the introduction of a standard reference reagent for MERS-CoV serology. Our study findings indicate that, when possible, laboratories should use a testing algorithm including >2 tests to ensure correct diagnosis of MERS-CoV. We also demonstrate that the use of a reference reagent greatly improves the agreement between assays, enabling more consistent and therefore more meaningful comparisons between results.</div>
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Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus.

Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus.

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