MersV1, Main, Exploration, bibRecordById, pubmed:14652182

Fraying and electron autodetachment dynamics of trapped gas phase oligonucleotides.

Identifieur interne : 003218 ( Main/Exploration ); précédent : 003217; suivant : 003219

Fraying and electron autodetachment dynamics of trapped gas phase oligonucleotides.

Auteurs : Allison S. Danell [États-Unis] ; Joel H. Parks

Source :

Journal of the American Society for Mass Spectrometry [ 1044-0305 ] ; 2003.

RBID : pubmed:14652182

Descripteurs français

KwdFr :
- Colorants fluorescents, Composés du bore, Modèles moléculaires, Nanotechnologie, Oligonucléotides (), Spectrométrie de fluorescence, Spectrométrie de masse ESI, Température, Électrons.
MESH :
- Colorants fluorescents, Composés du bore, Modèles moléculaires, Nanotechnologie, Oligonucléotides, Spectrométrie de fluorescence, Spectrométrie de masse ESI, Température, Électrons.

English descriptors

KwdEn :
- Boron Compounds, Electrons, Fluorescent Dyes, Models, Molecular, Nanotechnology, Oligonucleotides (chemistry), Spectrometry, Fluorescence, Spectrometry, Mass, Electrospray Ionization, Temperature.
MESH :
- chemical , chemistry : Oligonucleotides.
- chemical : Boron Compounds, Fluorescent Dyes.
- Electrons, Models, Molecular, Nanotechnology, Spectrometry, Fluorescence, Spectrometry, Mass, Electrospray Ionization, Temperature.

Abstract

Sensitive methods recently developed to measure laser-induced fluorescence from trapped ions have been applied to study the dynamics of double- and single-stranded oligonucleotides. In this paper, the fraying of duplex terminal base pairs has been identified by measuring the donor fluorescence as a function of temperature from an oligonucleotide duplex labeled with a pair of FRET dyes. Comparison of the degree of dissociation of 14-mer duplexes observed in the mass spectra with the fluorescence intensity of the donor enables intermediate conformations of the unzipping duplex at the weaker binding end of the duplex to be identified. The autodetachment of electrons from double- and single-stranded oligonucleotide anions has been observed in a gas phase environment. To characterize this process, measurements were performed on 7-mers prepared without FRET fluorophores attached. The dependence of the decay rates of trapped anions have been measured as a function of charge state and temperature for various base compositions. An exceptionally strong dependence of the decay rate on base composition has been identified. The physical basis for this process will be discussed.

DOI: 10.1016/S1044-0305(03)00578-6
PubMed: 14652182

Affiliations:

États-Unis

Le document en format XML

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<term>Fluorescent Dyes</term>
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<term>Spectrometry, Fluorescence</term>
<term>Spectrometry, Mass, Electrospray Ionization</term>
<term>Temperature</term>
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<term>Composés du bore</term>
<term>Modèles moléculaires</term>
<term>Nanotechnologie</term>
<term>Oligonucléotides</term>
<term>Spectrométrie de fluorescence</term>
<term>Spectrométrie de masse ESI</term>
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<front><div type="abstract" xml:lang="en">Sensitive methods recently developed to measure laser-induced fluorescence from trapped ions have been applied to study the dynamics of double- and single-stranded oligonucleotides. In this paper, the fraying of duplex terminal base pairs has been identified by measuring the donor fluorescence as a function of temperature from an oligonucleotide duplex labeled with a pair of FRET dyes. Comparison of the degree of dissociation of 14-mer duplexes observed in the mass spectra with the fluorescence intensity of the donor enables intermediate conformations of the unzipping duplex at the weaker binding end of the duplex to be identified. The autodetachment of electrons from double- and single-stranded oligonucleotide anions has been observed in a gas phase environment. To characterize this process, measurements were performed on 7-mers prepared without FRET fluorophores attached. The dependence of the decay rates of trapped anions have been measured as a function of charge state and temperature for various base compositions. An exceptionally strong dependence of the decay rate on base composition has been identified. The physical basis for this process will be discussed.</div>
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Serveur d'exploration MERS

Fraying and electron autodetachment dynamics of trapped gas phase oligonucleotides.

Fraying and electron autodetachment dynamics of trapped gas phase oligonucleotides.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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