Fraying and electron autodetachment dynamics of trapped gas phase oligonucleotides.
Identifieur interne : 003218 ( Main/Exploration ); précédent : 003217; suivant : 003219Fraying and electron autodetachment dynamics of trapped gas phase oligonucleotides.
Auteurs : Allison S. Danell [États-Unis] ; Joel H. ParksSource :
- Journal of the American Society for Mass Spectrometry [ 1044-0305 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Oligonucleotides.
- chemical : Boron Compounds, Fluorescent Dyes.
- Electrons, Models, Molecular, Nanotechnology, Spectrometry, Fluorescence, Spectrometry, Mass, Electrospray Ionization, Temperature.
Abstract
Sensitive methods recently developed to measure laser-induced fluorescence from trapped ions have been applied to study the dynamics of double- and single-stranded oligonucleotides. In this paper, the fraying of duplex terminal base pairs has been identified by measuring the donor fluorescence as a function of temperature from an oligonucleotide duplex labeled with a pair of FRET dyes. Comparison of the degree of dissociation of 14-mer duplexes observed in the mass spectra with the fluorescence intensity of the donor enables intermediate conformations of the unzipping duplex at the weaker binding end of the duplex to be identified. The autodetachment of electrons from double- and single-stranded oligonucleotide anions has been observed in a gas phase environment. To characterize this process, measurements were performed on 7-mers prepared without FRET fluorophores attached. The dependence of the decay rates of trapped anions have been measured as a function of charge state and temperature for various base compositions. An exceptionally strong dependence of the decay rate on base composition has been identified. The physical basis for this process will be discussed.
DOI: 10.1016/S1044-0305(03)00578-6
PubMed: 14652182
Affiliations:
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Le document en format XML
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<term>Nanotechnology</term>
<term>Oligonucleotides (chemistry)</term>
<term>Spectrometry, Fluorescence</term>
<term>Spectrometry, Mass, Electrospray Ionization</term>
<term>Temperature</term>
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<term>Composés du bore</term>
<term>Modèles moléculaires</term>
<term>Nanotechnologie</term>
<term>Oligonucléotides ()</term>
<term>Spectrométrie de fluorescence</term>
<term>Spectrométrie de masse ESI</term>
<term>Température</term>
<term>Électrons</term>
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<term>Fluorescent Dyes</term>
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<term>Nanotechnology</term>
<term>Spectrometry, Fluorescence</term>
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<term>Temperature</term>
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<term>Composés du bore</term>
<term>Modèles moléculaires</term>
<term>Nanotechnologie</term>
<term>Oligonucléotides</term>
<term>Spectrométrie de fluorescence</term>
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<front><div type="abstract" xml:lang="en">Sensitive methods recently developed to measure laser-induced fluorescence from trapped ions have been applied to study the dynamics of double- and single-stranded oligonucleotides. In this paper, the fraying of duplex terminal base pairs has been identified by measuring the donor fluorescence as a function of temperature from an oligonucleotide duplex labeled with a pair of FRET dyes. Comparison of the degree of dissociation of 14-mer duplexes observed in the mass spectra with the fluorescence intensity of the donor enables intermediate conformations of the unzipping duplex at the weaker binding end of the duplex to be identified. The autodetachment of electrons from double- and single-stranded oligonucleotide anions has been observed in a gas phase environment. To characterize this process, measurements were performed on 7-mers prepared without FRET fluorophores attached. The dependence of the decay rates of trapped anions have been measured as a function of charge state and temperature for various base compositions. An exceptionally strong dependence of the decay rate on base composition has been identified. The physical basis for this process will be discussed.</div>
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